Additional file 1. An Excel® spreadsheet file containing all three data tables that were collected and analysed in this study, anonymized by removing all variables containing information that could be used to identify individuals, households or their houses.

Additional file 1. Additional file

Two new halogenated metabolites, laurenhalogens A (1) and B (2), along with four known ones (3–6), were isolated from the red alga Laurencia sp. The structures of 1 and 2 were determined by the means of UV, IR, MS, NMR and X-ray diffraction analysis. In addition, the antibacterial activities of 1–6 were also evaluated.

Additional file 1.

Abstract Background Antiretroviral therapy (ART) is the primary mode of treatment for Human Immunodeficiency Virus (HIV). It slows disease progression and reduces the spread of infection. HIV treatment is also known to require a high level of adherence of over 90% to achieve good treatment outcomes and viral load suppression. In Sierra Leone, about 70% of People Living with HIV (PLHIV) are non-adherent in their first year of treatment. Understanding the reasons behind this high...

Additional file 2: Data extraction form. S2.1. Data extraction form.

Additional file 1: Figure S1. Restricted cubic splines analysis of the relationship between abdominal obesity indices and the risk of cardiovascular events among people with type 2 diabetes. Figure S2. Subdistribution hazard ratios for the association between abdominal obesity indices and cardiovascular events in people with type 2 diabetes. Table S1. Association between baseline abdominal obesity indices and incident cardiovascular events in people with type 2 diabetes. Table S2. Predictive performance of abdominal obesity indices...

Understanding selectivity-dependent molecular mechanism of inhibitors towards CDK2 over CDK6 is prominent for improving drug design towards the CDK family. Multiple short molecular dynamics (MD) simulations combined with MM-GBSA approach are adopted to investigate molecular mechanism on binding selectivity of inhibitors X64, X3A, and 4 AU to CDK2 and CDK6. The RMSF analysis and calculations of molecular surface areas indicate that local structural and global flexibility of CDK6 are stronger than that of CDK2. Based...

Additional file 1. STROBE Statement—Checklist of items that should be included in reports of cross-sectional studies Title of the study: Investigating health service availability and readiness for antenatal testing and treatment for HIV and syphilis in Papua New Guinea.

Additional file 3: Figure S2. The birth weight z-scores comparing STOPPAM vs. Intergrowth (IG21) references. The dash line indicates the mean birthweight z-scores.

Cascaded operations play an important role in traditional electronic computing systems for the realization of advanced strategies. Here, we introduce the idea of cascaded operations into all-optical spatial analog computing. The single function of the first-order operation is difficult to meet the requirements of practical applications in image recognition. The all-optical second-order spatial differentiators are implemented by cascading two first-order differential operation units, and the image edge detection of amplitude and phase objects are demonstrated....

Additional file 1: Appendix 1. Search strategy.

Additional file 1. Additional methods. Fig. S1. mTOR senses the availability of methionine. Fig. S2. mTORC1 signaling pathway mediated Myc expression to promote HCC tumorigenesis. Fig. S3. Negative correlation of MYC and SIRT4 expression. Fig. S4. Myc regulates SIRT4 stability through proteasome degradation. Fig. S5. Characterization of the interaction between SIRT4 and TRIM32. Fig. S6. SIRT4 suppresses Myc promoted HCC tumorigenesis. Fig. S7. The effect of SIRT4 on histone methylation through regulating methionine metabolism. Fig....

Additional file 1. Additional methods. Fig. S1. mTOR senses the availability of methionine. Fig. S2. mTORC1 signaling pathway mediated Myc expression to promote HCC tumorigenesis. Fig. S3. Negative correlation of MYC and SIRT4 expression. Fig. S4. Myc regulates SIRT4 stability through proteasome degradation. Fig. S5. Characterization of the interaction between SIRT4 and TRIM32. Fig. S6. SIRT4 suppresses Myc promoted HCC tumorigenesis. Fig. S7. The effect of SIRT4 on histone methylation through regulating methionine metabolism. Fig....

Additional file 2: Table S1. Detected gene levels upon methionine restriction in livers of HCC mouce models.

Abstract Background Exploiting cancer metabolism during nutrient availability holds immense potential for the clinical and therapeutic benefits of hepatocellular carcinoma (HCC) patients. Dietary methionine is a metabolic dependence of cancer development, but how the signal transduction integrates methionine status to achieve the physiological demand of cancer cells remains unknown. Methods Low or high levels of dietary methionine was fed to mouse models with patient-derived xenograft or diethyl-nitrosamine induced liver cancer. RNA sequence and metabolomics were...

Longitudinal measurement invariance (LMI) is a critical prerequisite to assessing change over time with intensive longitudinal data (ILD). For LMI testing with ILD, we propose cross-classified factor analysis (CCFA) to detect non-invariant item parameters and alignment optimization (AO) to detect non-invariant time points as a supplement to CCFA. In addition, we use a covariate in CCFA to identify a source of non-invariance. To evaluate the proposed models under unique features of ILD, such as autoregression...

Abstract Exosomes of human cardiosphere-derived cells (CDCs) are very promising for treating cardiovascular disorders. However, the current challenge is inconvenient delivery methods of exosomes for clinical application. The present study aims to explore the potential to enhance the therapeutic effect of exosome (EXO) from human CDCs to myocardial hypertrophy. A heart homing peptide (HHP) was displayed on the surface of exosomes derived from CDCs that were forced to express the HHP fused on the N-terminus...

Additional file 1: Figure S1. Schematic illustration of displaying a homing peptide/FLAG on the surface of exosomes. The coding sequence of HHP/FLAG (red bar) was fused in-frame to the LAMP2b cDNA between the signal peptide (SP) and the N-terminus, which was then cloned into pLVX-IRES-ZsGreen1 expression plasmid. Forced expression of the plasmid in CDCs would display the HHP/FLAG (red oval) on the surface of exosomes. Figure S2. Schematic illustration of mice treatment schedule. The TAC...

Additional file 1: Figure S1. Epidemic trajectory, vaccine uptake, and clinical severity of Hong Kong SAR’s Omicron wave. Figure S2. Vaccinations uptake by age in Hong Kong SAR, China, mainland China and New Zealand. Figure S3. Population immunity levels in the baseline immunization scenario and the enhanced immunization scenarios. Figure S4. The observed epidemic growth rate and effective reproduction numbers with baseline interventions in the early stage of the two recent outbreaks in mainland China....

Additional file 5. Fig. S32: The host assignment of plant-associated viruses by using BLASTx searching the contigs assembled from the NGS data of each plant species (those containing possible non-plant-infecting virus) against the total mitochondrial proteome database that were downloaded from GenBank.

Additional file 2. Fig. S1: Pictures of plant strains or leaves collected in the present study.

ObjectiveThis study sought to evaluate a modified palatoplasty technique (MPT) concerning the postoperative outcomes and associated influencing factors.DesignA retrospective cohort study.Participants and settingOne hundred forty-three consecutive patients with non-syndromic cleft palate, who received MPT before one year of age within an oral and maxillofacial surgery department of a university-affiliated tertiary hospital between 2011-2017, were reviewed.Main measuresThe postoperative wound healing and velopharyngeal function (VPF) were the primary outcome measures. The sex, age at surgery, cleft type,...

Additional file 1. Online Appendix.

We mistakenly diagnosed a patient with pulmonary embolism as ST-elevation acute myocardial infarction. The case complained of chest pain with ST-elevation in leads AVR, III, AVF, V1, V3R, V4R and V5R. In addition, we also found ST- elevation AVR, III>ST- elevation AVF, QIII>QAVF and QV1. Multi-site pulmonary embolism can lead to type 2 myocardial infarction or special type of myocardial infarction with non-obstructive coronary arteries through multiple pathological mechanisms.