19 Works

Data from: Multi-species genetic connectivity in a terrestrial habitat network

Robby R. Marrotte, Jeff Bowman, Michael G. C. Brown, Chad Cordes, Kimberley Y. Morris, Melanie B. Prentice & Paul J. Wilson
Background: Habitat fragmentation reduces genetic connectivity for multiple species, yet conservation efforts tend to rely heavily on single-species connectivity estimates to inform land-use planning. Such conservation activities may benefit from multi-species connectivity estimates, which provide a simple and practical means to mitigate the effects of habitat fragmentation for a larger number of species. To test the validity of a multi-species connectivity model, we used neutral microsatellite genetic datasets of Canada lynx (Lynx canadensis), American marten...

Data from: Cyclic bouts of extreme bradycardia counteract the highmetabolism of frugivorous bats

M. Teague O'Mara, Martin Wikelski, Christian C. Voigt, Andries Ter Maat, Henry S. Pollock, Gary Burness, Lanna M. Desantis, Dina K. N. Dechmann & Dina KN Dechmann
Active flight requires the ability to efficiently fuel bursts of costly locomotion while maximizing energy conservation during non-flying times. We took a multi-faceted approach to estimate how fruit-eating bats (Uroderma bilobatum) manage a high-energy lifestyle fueled primarily by fig juice. Miniaturized heart rate telemetry shows that they use a novel, cyclic, bradycardic state that reduces daily energetic expenditure by 10% and counteracts heart rates as high as 900 bpm during flight. Uroderma bilobatum support flight...

Data from: Evolutionary reconstruction supports the presence of a Pleistocene Arctic refugium for a large mammal species

Cornelya F. C. Klütsch, Micheline Manseau, Morgan Anderson, Peter Sinkins & Paul J. Wilson
Aim: The presence of refugia in the Canadian High Arctic has been subject to debate for decades. We investigated the potential existence of Arctic refugia during the Pleistocene for a large mammal species in the Canadian Archipelago because if these refugia were present, reconsideration of the evolutionary histories of North American fauna and flora beyond the major refugia of Beringia and south of the Laurentide and Cordilleran Ice Sheets would be required. Peary caribou (Rangifer...

Data from: Development of a genotype-by-sequencing immunogenetic assay as exemplified by screening for variation in red fox with and without endemic rabies exposure

Michael E. Donaldson, Yessica Rico, Karsten Hueffer, Halie M. Rando, Anna V. Kukekova & Christopher J. Kyle
Pathogens are recognized as major drivers of local adaptation in wildlife systems. By determining which gene variants are favored in local interactions among populations with and without disease, spatially explicit adaptive responses to pathogens can be elucidated. Much of our current understanding of host responses to disease comes from a small number of genes associated with an immune response. High-throughput sequencing (HTS) technologies, such as genotype-by-sequencing (GBS), facilitate expanded explorations of genomic variation among populations....

Data from: Genetic mating system and mate selection in smallmouth bass

Ryan P. Franckowiak, Mark S. Ridgway & Chris C. Wilson
Mating systems are an important factor influencing the variance in reproductive success among individuals within natural populations and thus have important ecological and evolutionary implications. We used molecular pedigree reconstruction techniques with microsatellite DNA data to characterize the genetic mating system and mate selection in adult smallmouth bass spawning in Lake Opeongo. The genetic mating system of smallmouth bass in this system can be characterized as predominantly monogamous with a low rate of polygynandry particularly...

Data from: Comparison of 454 pyrosequencing methods for characterizing the major histocompatibility complex of nonmodel species and the advantages of ultra deep coverage

Rebekah A. Oomen, Roxanne M. Gillett & Christopher J. Kyle
Characterization and population genetic analysis of multilocus genes, such as those found in the major histocompatibility complex (MHC) is challenging in nonmodel vertebrates. The traditional method of extensive cloning and Sanger sequencing is costly and time-intensive and indirect methods of assessment often underestimate total variation. Here, we explored the suitability of 454 pyrosequencing for characterizing multilocus genes for use in population genetic studies. We compared two sample tagging protocols and two bioinformatic procedures for 454...

Data from: Profiling the immunome of little brown myotis provides a yardstick for measuring the genetic response to white-nose syndrome

Michael E. Donaldson, Christina M. Davy, Craig K.R. Willis, Scott McBurney, Allysia Park, Christopher J. Kyle & Craig K. R. Willis
White-nose syndrome (WNS) has devastated populations of hibernating bats in eastern North America, leading to emergency conservation listings for several species including the previously ubiquitous little brown myotis (Myotis lucifugus). However, some bat populations near the epicenter of the white-nose syndrome panzootic appear to be stabilizing after initial precipitous declines, which could reflect a selective immunogenetic sweep. To investigate the hypothesis that WNS exerts significant selection on the immunome of affected bat populations, we developed...

Data from: Conservation genetics of redside dace (Clinostomus elongatus): phylogeography and contemporary spatial structure

Natasha R. Serrao, Scott M. Reid & Chris C. Wilson
Redside dace Clinostomus elongatus (Teleostei: Cyprinidae) is a species of conservation concern that is declining throughout its range as a result of habitat fragmentation, degradation and loss. We characterized the genetic structure and diversity of redside dace populations across the species range using mitochondrial and microsatellite data to inform conservation efforts and assess how historical and recent events have shaped genetic structure and diversity within and among populations. Phylogeographic structure among 28 redside dace populations...

Data from: Continental divide: predicting climate-mediated fragmentation and biodiversity loss in the boreal forest

Dennis L. Murray, Michael J.L. Peers, Yasmine N. Majchrzak, Morgan Wehtje, Catarina Ferreira, Rob S.A. Pickles, Jeffrey R. Row, Daniel H. Thornton, Michael J. L. Peers & Rob S. A. Pickles
Climate change threatens natural landscapes through shifting distribution and abundance of species and attendant change in the structure and function of ecosystems. However, it remains unclear how climate-mediated variation in species' environmental niche space may lead to large-scale fragmentation of species distributions, altered meta-population dynamics and gene flow, and disrupted ecosystem integrity. Such change may be especially relevant when species distributions are restricted either spatially or to a narrow environmental niche, or when environments are...

Data from: Signatures of selection in mammalian clock genes with coding trinucleotide repeats: implications for studying the genomics of high-pace adaptation

Melanie B. Prentice, Jeff Bowman, Jillian L. Lalor, Michelle M. McKay, Lindsay A. Thomson, Cristen M. Watt, Andrew G. McAdam, Dennis L. Murray & Paul J. Wilson
Climate change is predicted to affect the reproductive ecology of wildlife; however, we have yet to understand if and how species can adapt to the rapid pace of change. Clock genes are functional genes likely critical for adaptation to shifting seasonal conditions through shifts in timing cues. Many of these genes contain coding trinucleotide repeats, which offer the potential for higher rates of change than single nucleotide polymorphisms (SNPs) at coding sites, and, thus, may...

Data from: Body condition explains migratory performance of a long-distance migrant

Sjoerd Duijns, Lawrence J. Niles, Amanda Dey, Yves Aubry, Christian Friis, Stephanie Koch, Alexandra M. Anderson & Paul A. Smith
Body condition (i.e. relative mass after correcting for structural size) affects the behaviour of migrating birds, but how body condition affects migratory performance, timing and fitness is still largely unknown. Here, we studied the effects of relative body condition on individual departure decisions, wind selectivity, flight speed and timing of migration for a long-distance migratory shorebird, the red knot Calidris canutus rufa. By using automated VHF telemetry on a continental scale, we studied knots' migratory...

Data from: Prelude to a panzootic: gene flow and immunogenetic variation in northern little brown myotis vulnerable to bat white-nose syndrome

Christina M. Davy, Michael E. Donaldson, Yessica Rico, Cori L. Lausen, Kathleen Dogantzis, Kyle Ritchie, Craig K. R. Willis, Douglas W. Burles, Thomas S. Jung, Scott McBurney, Allysia Park, Donald J. McAlpine, Karen F. Vanderwolf, Christopher J. Kyle & Craig K.R. Willis
The fungus that causes bat white-nose syndrome (WNS) recently leaped from eastern North America to the Pacific Coast. The pathogen’s spread is associated with the genetic population structure of a host (Myotis lucifugus). To understand the fine-scale neutral and immunogenetic variation among northern populations of M. lucifugus, we sampled 1142 individuals across the species’ northern range. We used genotypes at 11 microsatellite loci to reveal the genetic structure of, and directional gene flow among, populations...

Data from: RAD sequencing and genomic simulations resolve hybrid origins within North American Canis

Linda Y. Rutledge, S. Devillard, J. Q. Boone, P. A. Hohenlohe & B. N. White
Top predators are disappearing worldwide, significantly changing ecosystems that depend on top-down regulation. Conflict with humans remains the primary roadblock for large carnivore conservation, but for the eastern wolf (Canis lycaon), disagreement over its evolutionary origins presents a significant barrier to conservation in Canada and has impeded protection for grey wolves (Canis lupus) in the USA. Here, we use 127 235 single-nucleotide polymorphisms (SNPs) identified from restriction-site associated DNA sequencing (RAD-seq) of wolves and coyotes,...

Data from: Sex-specific graphs: Relating group-specific topology to demographic and landscape data

Philip Bertrand, Jeff Bowman, Rodney Dyer, Micheline Manseau, Paul J. Wilson & Rodney J. Dyer
Sex-specific genetic structure is a commonly observed pattern among vertebrate species. Facing differential selective pressures, individuals may adopt sex-specific life history traits that ultimately shape genetic variation among populations. Although differential dispersal dynamics are commonly detected in the literature, few studies have used genetic structure to investigate sex-specific functional connectivity. The recent use of graph theoretic approaches in landscape genetics has demonstrated network capacities to describe complex system behaviours where network topology represents genetic interaction...

Data from: Ungulate predation and ecological roles of wolves and coyotes in eastern North America

John F. Benson, Karen M. Loveless, Linda Y. Rutledge & Brent R. Patterson
Understanding the ecological roles of species that influence ecosystem processes is a central goal of ecology and conservation biology. Eastern coyotes (Canis latrans) have ascended to the role of apex predator across much of eastern North America since the extirpation of wolves (Canis spp.) and there has been considerable confusion regarding their ability to prey on ungulates and their ecological niche relative to wolves. Eastern wolves (C. lycaon) are thought to have been the historical...

Data from: Tissue-specific transcriptome characterization for developing tadpoles of the northern leopard frog (Lithobates pipiens)

Jeffrey R. Row, Michael E. Donaldson, Jessica N. Longhi, Barry J. Saville & Dennis L. Murray
A potential cause of amphibian population declines are the impacts of environmental degradation on tadpole development. We conducted RNA sequencing on developing northern leopard frog tadpoles and through de novo transcriptome assembly we annotated a large number of open reading frames comparable in number and extent to genes identified in Xenopus. Using our transcriptome, we found transcript level changes between early (Gosner 26–31) and late (Gosner 36–41) stage tadpoles were the greatest in the tail,...

Data from: Selection and drift influence genetic differentiation of insular Canada lynx (Lynx canadensis) on Newfoundland and Cape Breton Island

Melanie B. Prentice, Jeff Bowman, Kamal Khidas, Erin L. Koen, Jeffrey R. Row, Dennis L. Murray & Paul J. Wilson
Island populations have long been important for understanding the dynamics and mechanisms of evolution in natural systems. While genetic drift is often strong on islands due to founder events and population bottlenecks, the strength of selection can also be strong enough to counteract the effects of drift. Here, we used several analyses to identify the roles of genetic drift and selection on genetic differentiation and diversity of Canada lynx (Lynx canadensis) across eastern Canada, including...

Data from: Compensatory selection for roads over natural linear features by wolves in northern Ontario: implications for caribou conservation

Erica J. Newton, Brent R. Patterson, Morgan L. Anderson, Arthur R. Rodgers, Lucas M. Vander Vennen & John M. Fryxell
Woodland caribou (Rangifer tarandus caribou) in Ontario are a threatened species that have experienced a substantial retraction of their historic range. Part of their decline has been attributed to increasing densities of anthropogenic linear features such as trails, roads, railways, and hydro lines. These features have been shown to increase the search efficiency and kill rate of wolves. However, it is unclear whether selection for anthropogenic linear features is additive or compensatory to selection for...

Data from: Establishing detection thresholds for environmental DNA using receiver operator characteristic (ROC) curves

Natasha R. Serrao, Scott M. Reid & Chris C. Wilson
Environmental DNA (eDNA) detection is increasingly being used to assess the potential presence of aquatic species. Setting defensible thresholds for qPCR assays are necessary to differentiate “detection” from “non-detection”, but threshold rationales are rarely reported in eDNA studies. Detection thresholds for data inclusion and exclusion have implications for downstream data analysis; uncritical acceptance could result in false positive results (Type I error), whereas overly conservative thresholds could result in high false negative interpretation (Type II...

Registration Year

  • 2017

Resource Types

  • Dataset


  • Trent University
  • Ontario Ministry of Natural Resources
  • Princeton University
  • University of Waterloo
  • University of Guelph
  • University of Winnipeg
  • University of Manitoba
  • University of Prince Edward Island
  • Instituto de Ecología
  • University of Illinois at Urbana Champaign