Additional file 1: Figure S1. Differential expression of genes encoding proteins that regulate MT organization in normal vs. OA and NOA human testes based on analyses of scRNA-Seq datasets. (A) scRNA-Seq datasets from normal vs. OA and NOA human testes were analyzed used Seurat package in R toolkit using Rstudio to obtain Dot plot to identify differentially expressed genes encoding genes pertinent to MT organization. It is noted that some of these proteins overlap with...

Additional file 1: Figure S1. Differential expression of genes encoding proteins that regulate MT organization in normal vs. OA and NOA human testes based on analyses of scRNA-Seq datasets. (A) scRNA-Seq datasets from normal vs. OA and NOA human testes were analyzed used Seurat package in R toolkit using Rstudio to obtain Dot plot to identify differentially expressed genes encoding genes pertinent to MT organization. It is noted that some of these proteins overlap with...

Abstract The importance of actin and microtubule (MT) cytoskeletons in testis function in rodents is known to some extent, but its role in the etiology of azoospermia in humans remains unexplored. Here, we examined if MT cytoskeleton was defective in NOA (non-obstructive azoospermia) testes versus normal human testes based on histopathological, immunofluorescence (IF), and scRNA-Seq transcriptome profiling. Testis biopsy samples from n = 6 normal men versus n = 3 Sertoli cell only (SCO) and...

Abstract The importance of actin and microtubule (MT) cytoskeletons in testis function in rodents is known to some extent, but its role in the etiology of azoospermia in humans remains unexplored. Here, we examined if MT cytoskeleton was defective in NOA (non-obstructive azoospermia) testes versus normal human testes based on histopathological, immunofluorescence (IF), and scRNA-Seq transcriptome profiling. Testis biopsy samples from n = 6 normal men versus n = 3 Sertoli cell only (SCO) and...