Tick-borne encephalitis virus (TBEV) accounts for approximately 10,000 annual cases of severe encephalitis in Europe and Asia and causes encephalitis in humans. In this study, we demonstrate TBEV appears to activate the interferon (IFN)-β dependent on RIG-I/MDA5. Both the IFN-β accumulation and the IFN stimulated genes (ISGs) transcription greatly delay. Further studies reveal that TBEV NS4A could block the phosphorylation and dimerization of STAT1/STAT2 to affect type I and II IFN-mediated STAT signalling. Additional data...

The interferon-inducible myxovirus resistance B (MxB) protein has been reported to inhibit HIV-1 and herpesviruses by blocking the nuclear import of viral DNA. Here, we report a new antiviral mechanism in which MxB restricts the nuclear import of HIV-1 regulatory protein Rev, and as a result, diminishes Rev-dependent expression of HIV-1 Gag protein. Specifically, MxB disrupts the interaction of Rev with the nuclear transport receptor, transportin 1 (TNPO1). Supporting this, the TNPO1-independent Rev variants become...

Candida albicans is a common fungal pathogen in humans that colonizes the skin and mucosal surfaces of the majority healthy individuals. How C. albicans disseminates into the bloodstream and causes life-threatening systemic infections in immunocompromised patients remains unclear. Plasminogen system activation can degrade a variety of structural proteins in vivo and is involved in several homeostatic processes. Here, for the first time, we characterized that C. albicans could capture and “subvert” host plasminogen to invade...

Heat stress (HS) leads to substantial economic loss of dairy industry each year. The negative effect of HS in dairy cows is becoming one of the more urgent issue due to accelerating side-effects of global warming. Various genes are involved in HS response but the information about the role of noncoding RNAs, especially circular RNAs (circRNAs) is largely unknown. In our study, we aimed to investigate the different expression profile of circRNAs between HS and...

Hallucinogenic mushroom is a kind of toxic strain containing psychoactive tryptamine substances such as psilocybin, psilocin and ibotenic acid, etc. The mushrooms containing hallucinogenic components are various, widely distributed and lack of standard to define, which made a great challenge to identification. Traditional identification methods, such as morphology and toxicology analysis, showed shortcomings in old or processed samples, while the DNA-based identification of hallucinogenic mushrooms would allow to identify these samples due to the stability...

Gallbladder cancer (GBC) is the common malignancy of the bile tract system with extremely poor clinical outcomes, owing to its metastatic property and intrinsic resistance to the first-line drugs. Although it is well-established that cholesterol abnormity contributes to gallstone formation, a leading risk factor for GBC, the link of cholesterol homeostasis with GBC has not been investigated. The present study systematically examined the genes implicated in cholesterol homeostasis, and revealed altered gene expressions of de...

Raw palygorskite (RPal) and its structural evolution products (OPal) were modified with silane coupling agent KH-570 to form silanized KRPal and KOPal nanorods, which were used as eco-friendly nanofillers to prepare polypropylene/KRPal (PP/KRPal) and polypropylene/KOPal (PP/KOPal) organic–inorganic composites. Structure characterization results revealed that moderate oxalic acid (OA) leaching process can break Si-O-M bonds (M represents Mg, Al, etc.) of RPal nanorods to produce more active silanol groups, thereby improving the dispersibility, surface reactivity and surface...

Raw palygorskite (RPal) and its structural evolution products (OPal) were modified with silane coupling agent KH-570 to form silanized KRPal and KOPal nanorods, which were used as eco-friendly nanofillers to prepare polypropylene/KRPal (PP/KRPal) and polypropylene/KOPal (PP/KOPal) organic–inorganic composites. Structure characterization results revealed that moderate oxalic acid (OA) leaching process can break Si-O-M bonds (M represents Mg, Al, etc.) of RPal nanorods to produce more active silanol groups, thereby improving the dispersibility, surface reactivity and surface...

The Cenozoic Beibuwan Basin is located along the eastern margin of the Ailao Shan Red River Shear Zone (ASRRSZ). The rifting and inversion processes of this basin provide important clues to the mechanism by which extrusion tectonics drive continental rifting and inversion development. However, the rifting and inversion of this basin remain ambiguous. Here, we employ seismic data, information about the time and displacement of faulting, syn-rift fault and isopach maps to analyse the Cenozoic...

The anterior cingulate cortex (ACC) is a core brain region processing pain emotion. In this study, we performed RNA sequencing analysis to reveal transcriptomic profiles of the ACC in a rat chronic constriction injury (CCI) model. A total of 1628 differentially expressed genes (DEGs) were identified by comparing sham-operated rats with rats of 12 hours, 1, 3, 7, and 14 days after surgery, respectively. Although these inflammatory-related DEGs were generally increased after CCI, different kinetics...

Recent research unveiled that LINC00857 plays a regulatory role in multiple human cancers, such as lung adenocarcinoma and gastric cancer. Nevertheless, the function of LINC00857 in pancreatic adenocarcinoma (PAAD) remains unclear. This study concentrates on LINC00857 to discuss the relevant molecular mechanism of this gene in PAAD. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) and western blot were implemented for measuring the expressions of RNAs and proteins. Wound healing and Transwell assays were used to...

Grey mould, caused by Botrytis cinerea, is an increasingly destructive disease of ginseng (Panax ginseng) in China. However, the extent of variation in the morphological characteristics of fungal colonies, genetic background and pathogenicity of different strains of this pathogen remains unknown. In this study, we isolated and identified 102 isolates of B. cinerea from ginseng. Among these, the sclerotial phenotype was dominant, followed by the mycelial and conidial phenotypes, with the proportion of each phenotype...

The present study aimed to assess the role of the long non-coding RNA-urothelial cancer associated 1 (lncRNA-UCA1)/microRNA (miR)-383/vascular endothelial growth factor A (VEGFA) axis in regulating lung adenocarcinoma physiology through in vivo and in vitro experiments. The expression profile of lncRNA-UCA1 was analyzed by genome-wide analysis from GSE146459. The cell counting Kit-8, colony formation, wound healing and transwell assays were performed to evaluate the effects of lncRNA-UCA1 in vitro. In addition, luciferase reporter assays were...

The reduction of CO2 into valuable chemicals through electrocatalytic techniques provides a potential strategy to alleviate energy crises and environmental pollution. In this study, 2D M3(THT)2 (M = Fe, Co, Ni, Ru, Rh, Pd, THT = 2,3,6,7,10,11-triphenylenehexathiolate) framework as CO2 reduction reaction (CO2RR) electrocatalysts was investigated by using the density functional method. The results showed that the studied catalysts are stable both thermodynamically and electrochemically. Rh3(THT)2 exhibits the best catalytic performance to produce CH4 with...

Additional file 1: Figure S1. Panx 2 and Panx 3 mRNA expression after CCI. A-B. A qPCR assay was used to detect Panx 2 (A) and Panx 3 (B) mRNA levels at 4 and 14 days post-CCI. The data are mean ± SEM. n = 3 mice/group. **p < 0.01, ***p < 0.001, vs. sham injury. The data were analyzed by one-way ANOVA followed by Dunnett’s test.

A coupled data-driven and 3-dimensional (3D) process-based fluorescent dissolved organic matter (fDOM) prediction model was developed for a shallow, subtropical Australian reservoir. The extent to which reservoir water volume, inflow, and wind conditions affect the fDOM transport dynamics during cyclonic weather events was assessed through scenario analysis and a data-driven Bayesian network (BN) approach. The analysis shows that (a) inflow plumes are the main sources of fDOM during heavy rainfall; (b) the concentration of fDOM...

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9 mediated gene editing systems have been used in a variety of organisms, including plants. This system can efficiently edit target genes to generate mutants, especially multiplex mutants, which are invaluable genetic materials for gene functional studies. Although the use of traditional hybridization technology to construct multiplex mutants has been widely used in plant research, the complex screening and identification, long construction cycle and other problems have always troubled...

Additional file 1. Characterization of DF formation by size, TEM and gel electrophoresis; colloidal stability of the nanoparticles; kinetics of drug release; catalytic multiple turn-over of the nanozymes; PDT effect; body weight change during treatments; H&E staining of the major organs.

Abstract Photodynamic therapy (PDT) has emerged as a promising tumor treatment method via light-triggered generation of reactive oxygen species (ROS) to kill tumor cells. However, the efficacy of PDT is usually restricted by several biological limitations, including hypoxia, excess glutathione (GSH) neutralization, as well as tumor resistance. To tackle these issues, herein we developed a new kind of DNA nanozyme to realize enhanced PDT and synergistic tumor ferroptosis. The DNA nanozyme was constructed via rolling...

Additional file 2: Fig. S2 Leaf- related QTLs on 32 linkage groups from a genetic linkage map of maternal parent (P2).

Mid-infrared (MIR) single-photon detection is emerging as an important technology for various applications. Superconducting nanowire single photon detectors (SNSPDs) fabricated with superconducting films with energy gaps of a few meV are natural broadband single-photon detectors. Recently, extending SNSPDs’ operation wavelengths into the MIR region is highly attractive. γ-Nb₄N₃ has a reduced N content and lower energy gap than the commonly used δ-NbN, making SNSPDs based on γ-Nb₄N₃ film more sensitive to low energy photons. We...

more details about the experiment

Additional file 3: Supplemental Table 3. Probes for fluorescence in situ hybridization and RNA pulldown, siRNAs, primers for qRT-PCR and vector construction.

Additional file 5: Supplemental Table 5. The potential circPVT1-interacting proteins in CNE2 cells identified by LC-MS/MS spectrometry after pull-down with the biontin-labeled circPVT1 probe.

Additional file 7: Supplemental Table 7. Proteomic analysis of circPVT1-regulated proteins in CNE2 cells after transfected with the circPVT1overexpression or empty vectors, followed by LC-MS/MS spectrometry.