Additional file 3.

Additional file 5.

Additional file 9.

Additional file 13.

Additional file 6: Fig. S5. Mitochondrial gene expression in the hypothalamus of Ahi1 KO mice was examined.

Additional file 8: Fig. S7. Dexamethasone induced depression-like behavior in mice.

Abstract Background Previous studies have shown that depression is often accompanied by an increase in mtDNA copy number and a decrease in ATP levels; however, the exact regulatory mechanisms remain unclear. Methods In the present study, Western blot, cell knockdown, immunofluorescence, immunoprecipitation and ChIP-qPCR assays were used to detect changes in the Ahi1/GR-TFAM-mtDNA pathway in the brains of neuronal Abelson helper integration site-1 (Ahi1) KO mice and dexamethasone (Dex)-induced mice to elucidate the pathogenesis of...

Abstract Background Previous studies have shown that depression is often accompanied by an increase in mtDNA copy number and a decrease in ATP levels; however, the exact regulatory mechanisms remain unclear. Methods In the present study, Western blot, cell knockdown, immunofluorescence, immunoprecipitation and ChIP-qPCR assays were used to detect changes in the Ahi1/GR-TFAM-mtDNA pathway in the brains of neuronal Abelson helper integration site-1 (Ahi1) KO mice and dexamethasone (Dex)-induced mice to elucidate the pathogenesis of...

ABSTRACT This paper discusses the design of a safflower picking robot control system and focuses on a navigation control subsystem based on multisensor fusion. A navigation subsystem, an identification and positioning subsystem, a picking subsystem, and a levelling subsystem are designed. The hardware and software of the navigation subsystem are designed in detail, and a multisensor fusion positioning method based on extended Kalman fusion technology is proposed. The accuracy and stability levels of different combined...

Supplementary Material 2

Stress annealing is utilized for enhancing the magnetic softness of Fe83–xCoxB10Si3C3P1 (x = 0–16) amorphous alloys with saturation polarization up to 1.75 T. All of the stress-annealed alloys exhibit improved soft-magnetic properties, including low coercivity of 1.8–2.2 A/m, low core loss of 0.09–0.11 W/kg at 1.0 T and 50 Hz, and high permeability of 27,000–33,200 at 5 A/m and 1 kHz. Stress annealing induces longitudinal magnetic anisotropy and facilitates the annihilation of free volume, leading...

Additional file 3: Supplementary Table S3. List of 30 the most dysregulated mRNAs in K-iPSC lines compared to AF-iPSC lines.

Additional file 4: Supplementary Table S4. List of 30 the most dysregulated mRNAs in K-NSC lines compared to AF-NSC lines.

Additional file 5: The karyotype of the K-iPSCs and AF-iPSCs were inspected by Giemsa-banding, and the results showed normal diploid 46, XY karyotype, without any detectable abnormalities at 10 passages.

Additional file 5: The karyotype of the K-iPSCs and AF-iPSCs were inspected by Giemsa-banding, and the results showed normal diploid 46, XY karyotype, without any detectable abnormalities at 10 passages.

Additional file 1: Supplementary Table S1. Antibodies used for immunocytochemistry.

Abstract Background Globoid cell leukodystrophy (GLD) is a devastating neurodegenerative disease characterized by widespread demyelination caused by galactocerebrosidase defects. Changes in GLD pathogenesis occurring at the molecular level have been poorly studied in human-derived neural cells. Patient-derived induced pluripotent stem cells (iPSCs) are a novel disease model for studying disease mechanisms and allow the generation of patient-derived neuronal cells in a dish. Results In this study, we identified gene-expression changes in iPSCs and iPSC-derived neural...

Additional file 2: Table S1. Independent genome-wide significant lead SNPs associated with pulmonary embolism (PE) in the discovery stage and replication stage. Table S2. The allele frequency of identified loci in 1000Genomes. Table S3. Replication of associations for the known loci in our cohort. Table S4. Association results for genes that were significant in FUMA gene-based analysis. Table S5. Polygenic risk score variants. Table S6. Polygenic risk score (PRS) quantile and odds ratio (OR).

Surface plasmon excitations in metallic hole array have been extensively studied in the context of light-matter interaction since the generated Bloch surface plasmon polariton (Bloch-SPP) exhibits unique properties of nanoscale light confinement, near-field enhancements, and long-range metal surface propagation. In this work, we experimentally demonstrate a plasmonic device that exhibits highly directional emission in visible light, using Ag film with thickness of 100 nm is deposited on the subwavelength porous alumina array as a plasmonic...

Clean revised manuscript

Additional file 1: Supplementary Figure 1. m6A modification levels in the Hippo pathway and metabolism in breast cancer. (a) Venn diagram (left panel) of common (the intersection area) and unique peaks (non-intersection region) of another pair of breast cancer tissue compared with adjacent normal tissue, |log2(fold change)|>1 and q value<0.05. The gene region (right panel) annotated by difference Peaks of breast cancer tissue compared with adjacent normal tissue, |log2(fold change)|>1 and q value<0.05. (b) Differential...

Additional file 2: Supplementary Table 1. Data of the included patients. Clinicopathological information on age, sex, clinical stage, neoadjuvant therapy, adjuvant therapy, ER, PR, HER2, Ki67, histological subtype and histological grade of each included patient. Supplementary Table 2. Differential m6A sites in two groups. Specific sites of 1850 up regulated and 1807 down regulated genes of breast cancer cells, p-values <0.05 were indicated. Supplementary Table 3. The relationship between METTL3 and patient clinicopathological features. The...

Abstract Background Non-small cell cancer (NSCLC) patients with concomitant epidermal growth factor receptor (EGFR) and TP53 mutations have a poor prognosis with the treatment of tyrosine kinase inhibitors (TKIs), and may benefit from a combination regimen preferentially. The present study aims to compare the benefits of EGFR-TKIs and its combination with antiangiogenic drugs or chemotherapy in patients with NSCLC harboring EGFR and TP53 co-mutation in a real-life setting. Methods This retrospective analysis included 124 patients...

Supplemental material, sj-docx-1-eso-10.1177_23969873231164790 for Improving neurological outcome for acute basilar artery occlusion with sufficient recanalization after thrombectomy by intraarterial tenecteplase (INSIST-IT): Rationale and design by Liang Liu, Wei Li, Jing Qiu, Thanh N Nguyen, Ming Wei, Feng Wang, Di Li, Huai-Zhang Shi, Shou-Chun Wang and Hui-Sheng Chen in European Stroke Journal

Background:The potential benefit of intraarterial tenecteplase in acute basilar artery occlusion (BAO) patients with successful reperfusion following endovascular treatment (EVT) has not been studied.Aims:To explore the efficacy and safety of intraarterial tenecteplase in acute BAO patients with successful reperfusion after EVT.Sample size estimates:A maximum of 228 patients are required to test the superiority hypothesis with 80% power according to a two-side 0.05 level of significance, stratified by center.Design:We will conduct a prospective, randomized, adaptive-enrichment, open-label,...