Abstract Background Cancer-associated fibroblasts (CAFs) are considered to play a fundamental role in pancreatic ductal adenocarcinoma (PDAC) progression and chemoresistance. Patient-derived organoids have demonstrated great potential as tumor avatars for drug response prediction in PDAC, yet they disregard the influence of stromal components on chemosensitivity. Methods We established direct three-dimensional (3D) co-cultures of primary PDAC organoids and patient-matched CAFs to investigate the effect of the fibroblastic compartment on sensitivity to gemcitabine, 5-fluorouracil and paclitaxel treatments...

Cytopenia is one of the most common adverse events following the CAR-T cell infusion, affecting the quality of life and potentially leading to life-threatening bleeding and infection. This study aimed to systematically review the cytopenias following anti-CD19 CAR-T therapy and further analyse the contributing factors. Databases including PubMed, MEDLINE, Embase and Cochrane were systematically searched on 8 May 2022. A random-effect meta-analysis was used to estimate the incidence of cytopenia, and subgroup analyses were applied...

Cytopenia is one of the most common adverse events following the CAR-T cell infusion, affecting the quality of life and potentially leading to life-threatening bleeding and infection. This study aimed to systematically review the cytopenias following anti-CD19 CAR-T therapy and further analyse the contributing factors. Databases including PubMed, MEDLINE, Embase and Cochrane were systematically searched on 8 May 2022. A random-effect meta-analysis was used to estimate the incidence of cytopenia, and subgroup analyses were applied...

Additional file 9. Table S9A–D. Over-represented KEGG pathway among gene clusters in MaMe21, MaMel63a, HEM, and HDF.

Additional file 1. Table S1. Normalized gene expression in MaMel cell lines.

Additional file 1. Table S1. Normalized gene expression in MaMel cell lines.

We consider the setting of an aggregate data meta-analysis of a continuous outcome of interest. When the distribution of the outcome is skewed, it is often the case that some primary studies report the sample mean and standard deviation of the outcome and other studies report the sample median along with the first and third quartiles and/or minimum and maximum values. To perform meta-analysis in this context, a number of approaches have recently been developed...

Additional file 1: Supplementary Figure 1. Flow diagram of patients included in the analysis set from GeparTrio study.

Additional file 2: Supplementary Figure 2. Distribution of EVI1 continuous expression in breast cancer: A: Histogram of EVI1 continuous expression in the entire cohort (N=993); B: Boxplots of EVI1 continuous expression within breast cancer subtypes. Note, 882/993 patients with available BC subtype information were included in this analysis. Horizontal bold lines present EVI1 median values of 116.65 in HR+/HER2-, 98.67 in HR+/HER2+, 114.77in HR-/HER2+, and 115.64 in TNBC; boxes present the interquartile range between first...

Abstract Background Many researchers have noticed that there is an increasing trend of Bielschowsky acquired comitant esotropia (ACE) in recent years related to excessive near work, but the exact pathogenesis and treatment methods have not been reported yet. Therefore, we aimed to characterize the clinical features of this ACE in adults and to evaluate the efficacy of botulinum toxin (BTX) injections in these patients. Methods This was a prospective consecutive case series of 47 patients...

Additional file 12: Figure S7. This figure shows the results of the analysis of biomarker expression in normal and tumor kidney by immunohistochemistry based on HPA data.

Additional file 1: Figure S1. Boxplot showing MFIs of all Isotype antibody controls.

Additional file 2: Figure S2. Boxplot showing MFIs of all mouse Isotype antibody controls.

Additional file 2: Figure S2. Boxplot showing MFIs of all mouse Isotype antibody controls.

Abstract Background and purpose Tumor-infiltrating lymphocytes (TILs) are associated with locoregional control (LRC) in head-and-neck squamous cell carcinoma (HNSCC) patients undergoing (chemo)radiotherapy. As immunosenescence results in reduced immune activity, the role of TILs in elderly HNSCC patients may differ compared to younger patients, providing a rationale to study the prognostic role of TILs and immune checkpoints (ICs) in this population. Material and methods Sixty-three HNSCC patients aged ≥ 65 years undergoing definitive (chemo)radiotherapy between 2010...

Additional file 8. Table S8A–D. Over-represented biological processes (GO-bp) among gene clusters in MaMe21, MaMel63a, HEM, and HDF.

Abstract Background Current therapeutic management of advanced melanoma patients largely depends on their BRAF mutation status. However, the vast heterogeneity of the tumors hampers the success of therapies targeting the MAPK/ERK pathway alone. Dissecting this heterogeneity will contribute to identifying key players in the oncogenic progression to tailor more effective therapies. Methods We performed a comprehensive molecular and phenotypic characterization of a panel of patient-derived BRAFV600E-positive melanoma cell lines. Transcriptional profiling was used to identify...

Additional file 7. Table S7A–D. Clustering of most variable genes in MaMe21, MaMel63a, HEM, and HDF.

Additional file 2: Table S2. Table showing the complete sLGGs cohort emphasizing the original histology before molecular pathology reevaluation, anatomical location, and molecular-biology data.

Additional file 3: Fig. S1. A total number of pediatric intracranial LGG patients with known genetic alteration is dividedby anatomical location. Importantly, KIAA1549:BRAF ex9:ex10 variant fusion was detected solely in the upperspine.

Additional file 1. Supplementary methods also including the following tables and figures Table S2. List of BCAC studies (including ABCS source) with the corresponding country and geographic area. Table S4: Clinical utility of the 5-year contralateral breast cancer risk prediction models (PredictCBC-1A with PredictCBC-2.0A and PredictCBC-1B with PredictCBC-2.0B). Figure S1. Visual assessment of calibration through calibration plots in the internal–external cross-validation at 5 years for the PredictCBC-2.0A model. Figure S2. Visual assessment of calibration through...

Additional file 2: Table S1. Description of the studies included in the analyses.

Additional file 1: Figure S1. 13C and DEPT NMR spectrum tobindole A (1). Figure S2. 1H NMR spectrum of tobindole A (1). Figure S3. HSQC NMR spectrum of tobindole A (1). Figure S4. HMBC NMR spectrum of tobindole A (1). Figure S5. HRMS spectrum of tobindole A (1). Figure S6. UV spectrum of tobindole A (1). Figure S7. IR spectrum of tobindole A (1). Figure S8. 13C and DEPT NMR spectrum tobindole B (2). Figure...

Additional file 1: Figure S1. 13C and DEPT NMR spectrum tobindole A (1). Figure S2. 1H NMR spectrum of tobindole A (1). Figure S3. HSQC NMR spectrum of tobindole A (1). Figure S4. HMBC NMR spectrum of tobindole A (1). Figure S5. HRMS spectrum of tobindole A (1). Figure S6. UV spectrum of tobindole A (1). Figure S7. IR spectrum of tobindole A (1). Figure S8. 13C and DEPT NMR spectrum tobindole B (2). Figure...

Additional file 13: Table S8. The annotation and homologs in Arabidopsis of these genes showing strong correlation with the lncRNAs were summarized in sunflower.