8 Works

Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

Aura Maria Moreno-Echeverri, Eva Susnik, Dimitri Vanhecke, Patricia Taladriz-Blanco, Sandor Balog, Alke Petri-Fink & Barbara Rothen-Rutishauser
Abstract Background In the field of nanoscience there is an increasing interest to follow dynamics of nanoparticles (NP) in cells with an emphasis on endo-lysosomal pathways and long-term NP fate. During our research on this topic, we encountered several pitfalls, which can bias the experimental outcome. We address some of these pitfalls and suggest possible solutions. The accuracy of fluorescence microscopy methods has an important role in obtaining insights into NP interactions with lysosomes at...

Ultrasensitive dopamine detection with graphene aptasensor multitransistor arrays

Mafalda Abrantes, Diana Rodrigues, Telma Domingues, Siva S. Nemala, Patricia Monteiro, Jérôme Borme, Pedro Alpuim & Luis Jacinto
Abstract Detecting physiological levels of neurotransmitters in biological samples can advance our understanding of brain disorders and lead to improved diagnostics and therapeutics. However, neurotransmitter sensors for real-world applications must reliably detect low concentrations of target analytes from small volume working samples. Herein, a platform for robust and ultrasensitive detection of dopamine, an essential neurotransmitter that underlies several brain disorders, based on graphene multitransistor arrays (gMTAs) functionalized with a selective DNA aptamer is presented. High-yield...

Additional file 1 of Ultrasensitive dopamine detection with graphene aptasensor multitransistor arrays

Mafalda Abrantes, Diana Rodrigues, Telma Domingues, Siva S. Nemala, Patricia Monteiro, Jérôme Borme, Pedro Alpuim & Luis Jacinto
Additional file 1: Figure S1. Raman spectrum of CVD grown single-layer graphene. Figure S2. Averaged response from measurement replicates of a single gMTA chip. Figure S3. XPS peak fitting for PBASE and PBASE + aptamer samples. Figure S4. Blank samples measurements in PBS. Figure S5. Stability of gMTA measurements. Figure S6. Dopamine attomolar detection with short incubation time.

Additional file 1 of Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

Aura Maria Moreno-Echeverri, Eva Susnik, Dimitri Vanhecke, Patricia Taladriz-Blanco, Sandor Balog, Alke Petri-Fink & Barbara Rothen-Rutishauser
Additional file 1: Figure S1A. Comparison of the absorbance and emission spectra of the different LysoTracker probes. Figure S1B. Comparison of the emission spectra of the different (nano)particles. Figure S1C. Comparison of the absorbance spectra of the different (nano)particles. Figure S2. Confocal microscopy images of different LysoTracker probes. Figure S3. Transmission electron micrographs (TEM) of the different silica (nano)particles. Figure S4A. Size distributions of 59 nm SiO2-BDP FL NP and 59 nm SiO2-RhoB NP measured...

Additional file 1 of Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

Aura Maria Moreno-Echeverri, Eva Susnik, Dimitri Vanhecke, Patricia Taladriz-Blanco, Sandor Balog, Alke Petri-Fink & Barbara Rothen-Rutishauser
Additional file 1: Figure S1A. Comparison of the absorbance and emission spectra of the different LysoTracker probes. Figure S1B. Comparison of the emission spectra of the different (nano)particles. Figure S1C. Comparison of the absorbance spectra of the different (nano)particles. Figure S2. Confocal microscopy images of different LysoTracker probes. Figure S3. Transmission electron micrographs (TEM) of the different silica (nano)particles. Figure S4A. Size distributions of 59 nm SiO2-BDP FL NP and 59 nm SiO2-RhoB NP measured...

Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

Aura Maria Moreno-Echeverri, Eva Susnik, Dimitri Vanhecke, Patricia Taladriz-Blanco, Sandor Balog, Alke Petri-Fink & Barbara Rothen-Rutishauser
Abstract Background In the field of nanoscience there is an increasing interest to follow dynamics of nanoparticles (NP) in cells with an emphasis on endo-lysosomal pathways and long-term NP fate. During our research on this topic, we encountered several pitfalls, which can bias the experimental outcome. We address some of these pitfalls and suggest possible solutions. The accuracy of fluorescence microscopy methods has an important role in obtaining insights into NP interactions with lysosomes at...

Additional file 1 of Ultrasensitive dopamine detection with graphene aptasensor multitransistor arrays

Mafalda Abrantes, Diana Rodrigues, Telma Domingues, Siva S. Nemala, Patricia Monteiro, Jérôme Borme, Pedro Alpuim & Luis Jacinto
Additional file 1: Figure S1. Raman spectrum of CVD grown single-layer graphene. Figure S2. Averaged response from measurement replicates of a single gMTA chip. Figure S3. XPS peak fitting for PBASE and PBASE + aptamer samples. Figure S4. Blank samples measurements in PBS. Figure S5. Stability of gMTA measurements. Figure S6. Dopamine attomolar detection with short incubation time.

Ultrasensitive dopamine detection with graphene aptasensor multitransistor arrays

Mafalda Abrantes, Diana Rodrigues, Telma Domingues, Siva S. Nemala, Patricia Monteiro, Jérôme Borme, Pedro Alpuim & Luis Jacinto
Abstract Detecting physiological levels of neurotransmitters in biological samples can advance our understanding of brain disorders and lead to improved diagnostics and therapeutics. However, neurotransmitter sensors for real-world applications must reliably detect low concentrations of target analytes from small volume working samples. Herein, a platform for robust and ultrasensitive detection of dopamine, an essential neurotransmitter that underlies several brain disorders, based on graphene multitransistor arrays (gMTAs) functionalized with a selective DNA aptamer is presented. High-yield...

Registration Year

  • 2022
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  • Collection
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  • Text
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Affiliations

  • International Iberian Nanotechnology Laboratory
    8
  • Adolphe Merkle Institute
    4
  • University of Fribourg
    4
  • University of Minho
    4
  • University of Porto
    4