Supplemental material, sj-pdf-1-prf-10.1177_02676591211031468 for Establishment of a venovenous extracorporeal membrane oxygenation in a rat model of acute respiratory distress syndrome by Yongnan Li, Jian Huang, Rongzhi Zhang, Shixiong Wang, Xingdong Cheng, Pengbin Zhang, Kerong Zhai, Wei Wang, Debin Liu and Bingren Gao in Perfusion

Supplemental material, sj-pdf-1-prf-10.1177_02676591211031468 for Establishment of a venovenous extracorporeal membrane oxygenation in a rat model of acute respiratory distress syndrome by Yongnan Li, Jian Huang, Rongzhi Zhang, Shixiong Wang, Xingdong Cheng, Pengbin Zhang, Kerong Zhai, Wei Wang, Debin Liu and Bingren Gao in Perfusion

Although members of the Mycobacterium tuberculosis complex (MTBC) exhibit high similarity, they are characterized by differences with respect to virulence, immune response, and transmissibility. To understand the virulence of these bacteria and identify potential novel therapeutic targets, we systemically investigated the total cell protein contents of virulent H37Rv, attenuated H37Ra, and avirulent M. bovis BCG vaccine strains at the log and stationary phases, based on tandem mass tag (TMT) quantitative proteomics. Data analysis revealed that...

One novel naphthalene derivative, 2-octa-2′,4′,6′-atriynenaphthalene (1), together with eighteen known compounds (2–19) were isolated from the aerial parts of Dracocephalum moldavica L. Compounds 2, 8, 10, 13, 15–17 and 19 were obtained from the family Lamiaceae for the first time, and compounds 11 and 18 were firstly identified from the genus of Dracocephalum. All the isolates were evaluated for anti-complementary activities through the classical and alternative pathways, and the targets of the most active compounds...

Heat stress is an environmental challenge that reduces plant productivity and growth. Plants have developed corresponding mechanisms to survive this adverse environmental stress. To demonstrate the mechanisms of how plants adapt to the environmental challenge, the heat response experiments involving Arabidopsis thaliana were retrieved from the GEO database. After quantile normalization of the GEO raw data, the differentially expressed genes (DEGs) in response to heat stress were identified by robust rank aggregation (RRA) algorithm, including...

To date, several different types of synthetic genetic switches, including riboregulators, riboswitches, and toehold switches, have been developed to construct AND, OR, NOT, NAND, NOR, and NOT IMPLICATION (NIMP) gates. The logic gate can integrate multiple input signals following a set of algorithms and generate a response only if strictly defined conditions are met. However, there are still some logic gates that have not been implemented but are necessary to build complex genetic circuits. Here,...

Hepatitis B virus (HBV) middle surface antigen (MHBs) mutation or deletion occurs in patients with chronic HBV infection. However, the functional role of MHBs in HBV infection is still an enigma. Here, we reported that 7.33% (11/150) isolates of CHB patients had MHBs start codon mutations compared with 0.00% (0/146) in acute hepatitis B (AHB) patients. Interestingly, MHBs loss accounted for 11.88% (126/1061) isolates from NCBI GenBank, compared with 0.09% (1/1061) and 0.00% (0/1061) for...

Long non-coding RNAs (lncRNAs) are involved in developing hepatocellular carcinoma (HCC). The present study explored the role of lncRNA LINC01194, which is upregulated in HCC tissues and might be a vital regulator in HCC progression. Levels of LINC01194, microRNA (miR)-655-3p, and SMAD family member 5 (SMAD5) were assessed using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The bioactivity of Huh-7 cells was assessed using cell counting kit-8 and transwell assays and flow cytometry. Western...

Dengue virus infection mainly causes dengue hemorrhagic fever (DHF) and/or dengue shock syndrome (DSS). However, ADE (antibody-dependent enhancement) is one of the main pathogenic factors, and its pathogenic mechanism has not been fully elucidated. Recently, with the development of high-throughput sequencing, an increased number of RNAs have been confirmed to play a vital regulatory role in the process of virus infection. However, there is a lack of research on dengue virus infection and ADE. In...

The research objects of this paper are Vigna angularis, Vigna radiata and Phaseolus vulgaris, which are important grain legume resources in China. However, the genetic structure between the three species is not clear. Therefore, this study developed a set of universal gSSR (simple sequence repeat) primers based on the whole-genome sequences of adzuki beans, mung beans and common beans and analysed the genetic diversity of the test resources based on universal gSSR loci. A total...

The research objects of this paper are Vigna angularis, Vigna radiata and Phaseolus vulgaris, which are important grain legume resources in China. However, the genetic structure between the three species is not clear. Therefore, this study developed a set of universal gSSR (simple sequence repeat) primers based on the whole-genome sequences of adzuki beans, mung beans and common beans and analysed the genetic diversity of the test resources based on universal gSSR loci. A total...

To obtain additional insight into the genetic factors of attention deficit hyperactivity disorder (ADHD). First, we performed a transcriptome-wide association study (TWAS) integrating human cerebellum-specific variant-expression/splicing correlations to identify ADHD susceptibility genes. Then, the associations between expression/splicing quantitative trait loci (eQTLs/sQTLs) of the transcriptome-wide significant genes and ADHD were observed in a case-control study of Han Chinese children. Furthermore, dual luciferase reporter gene assays were performed to validate the regulatory function of ADHD risk variants....

SOCS3 is low-expressed in breast cancer and may be a potential target. Ultrasound targeted microbubble destruction (UTMD) improved the efficiency of gene transfection. We explored the effects of UTMD-mediated transfection of SOCS3 on the biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells (BCSCs). The expression of SOCS3 in breast cancer (BC) and its association with prognosis were evaluated by GEPIA and The Cancer Genome Atlas (TCGA) websites. BCSCs were sorted by flow...

Bupleurum chinense DC. is a perennial herb plant belonging to the Umbelliferae family, genus Bupleurum, that is used as a traditional Chinese medicine. Coumarins are characteristic secondary metabolites of the Apiaceous family, which possess anti-inflammatory, anti-cancer and antiviral activities, among others. Few studies on the coumarin biosynthesis pathways in B. Chinese have been reported. In this study, we identified 89 differentially expressed genes belonging to 7 gene families involved in the synthesis of coumarins in...

The blue light hazard (BLH) and luminous efficacy of a source (ηV) are associated with the photobiological safety and energy saving of light-emitting diodes (LEDs), respectively. In this study, we used genetic algorithm (GA) to optimize the BLH, ηV, and color rendering parameters of five-chip hybrid white LEDs. Based on the optimal results, we used five-chip LEDs to obtain the hybrid white light. Their peak wavelengths were 461.5 (blue), 523.9 (green), 588.2 (orange), 643.3 (red),...

The blue light hazard (BLH) and luminous efficacy of a source (ηV) are associated with the photobiological safety and energy saving of light-emitting diodes (LEDs), respectively. In this study, we used genetic algorithm (GA) to optimize the BLH, ηV, and color rendering parameters of five-chip hybrid white LEDs. Based on the optimal results, we used five-chip LEDs to obtain the hybrid white light. Their peak wavelengths were 461.5 (blue), 523.9 (green), 588.2 (orange), 643.3 (red),...

As one of the three yield components, kernel number per spike (KNPS) plays a key role in determining wheat yield. In this study, in combination with the previously constructed genetic map for a tetraploid wheat recombinant inbred line (RIL) population developed from the cross between a durum wheat and a wild emmer wheat and phenotype data from four environments, two major and stably expressed quantitative trait loci (QTL) for KNPS were identified. The phenotypic variation...

The late Paleozoic tectonic evolution of the Lhasa terrane and subduction of Sumdo Paleo-Tethys ocean remains controversial due to limited data and variable Permian magmatism in central and southern Lhasa. We report new zircon U–Pb ages and Hf-isotope ratios as well as whole-rock major element, trace element, and Sr–Nd isotope data from middle Permian (266 ~ 257 Ma) arc-related magmatism, including high-Mg quartz diorite and trondhjemite in the Tangjia-Sumdo area. The quartz diorite has high...

Directed acyclic graph (DAG) models are widely used to represent casual relationships among random variables in many application domains. This article studies a special class of non-Gaussian DAG models, where the conditional variance of each node given its parents is a quadratic function of its conditional mean. Such a class of non-Gaussian DAG models are fairly flexible and admit many popular distributions as special cases, including Poisson, Binomial, Geometric, Exponential, and Gamma. To facilitate learning,...

Atg11 is an adaptor protein required for the induction of selective autophagy via receptor binding. However, our understanding of the molecular mechanisms by which it regulates selective autophagy remains incomplete. Here, we show that Atg11 is phosphorylated by Atg1. Rapamycin treatment or starvation conditions induced slower electrophoretic mobility of Atg11 in an Atg1 kinase activity-dependent manner. Through in vitro kinase assays combined with mutagenesis, we determined that Atg1 phosphorylates S949, S1057, and S1064 residues in...

Atg11 is an adaptor protein required for the induction of selective autophagy via receptor binding. However, our understanding of the molecular mechanisms by which it regulates selective autophagy remains incomplete. Here, we show that Atg11 is phosphorylated by Atg1. Rapamycin treatment or starvation conditions induced slower electrophoretic mobility of Atg11 in an Atg1 kinase activity-dependent manner. Through in vitro kinase assays combined with mutagenesis, we determined that Atg1 phosphorylates S949, S1057, and S1064 residues in...

Stress is a common factor that reduces production performance. However, the physiology of stress is not fully understood. In this study, 40-day-old rabbits were injected 1.0 mg/kg BW Dexamethasone to induce stress. A metabolomic study on serum of stress-induced rabbits was performed to research the metabolism mechanism of stress by using ultra-performance liquid chromatography coupled to mass spectrometry (UPLC–MS). Clear separations between stress rabbits and control rabbits were observed by principal component analysis (PCA) based...

Stress is a common factor that reduces production performance. However, the physiology of stress is not fully understood. In this study, 40-day-old rabbits were injected 1.0 mg/kg BW Dexamethasone to induce stress. A metabolomic study on serum of stress-induced rabbits was performed to research the metabolism mechanism of stress by using ultra-performance liquid chromatography coupled to mass spectrometry (UPLC–MS). Clear separations between stress rabbits and control rabbits were observed by principal component analysis (PCA) based...

Sustained cardiac hypertrophy (CH) contributes to many heart diseases. Long noncoding RNAs (lncRNAs) collectively play critical roles in cardiovascular diseases (CVDs). However, the roles of lncRNA H19 in CH are still unclear. A CH model was constructed utilizing isoproterenol (ISO). We demonstrated H19 could participate in regulating ISO-induced CH development both in vivo and in vitro. The online databases DIANA and TargetScan were used to predict the targets of H19 and MicroRNA-145-3p (miR-145-3p), respectively. Luciferase...

Additional file 1. Supplementary Material for a Successful Hybrid Deep Learning Model aiming at Promoter Identification.