Abstract Background Exosomes are deemed to be an important tool of intercellular communicators in cancer cells. Our study investigated the role of PRR34 long non-coding RNA antisense RNA 1 (PRR34-AS1) in regulating exosome secretion in hepatocellular carcinoma (HCC) cells. Methods Quantitative real-time polymerase chain reaction (RT-qPCR) analyzed the expression of PRR34-AS1. We assessed the function of PRR34-AS1 on the biological changes of THLE-3 cells and HCC cells. The downstream interaction between RNAS was assessed by...

Additional file 1: Supplementary Figure 1. MMP-13 levels in the medium of IL-1β-induced C28/I2 cells detected by ELISA.

Supplementary Material 1: Table S9 The HLR within 118 worldurban areas. Notes: The 118 world urban areas are selected onthe basis of area larger than 100 km2, being capitals, with populationsgreater than 1 million or have carbon monitoring sites.

Abstract Background The CO2 released by humans and livestock through digestion and decomposition is an important part of the urban carbon cycle, but is rarely considered in studies of city carbon budgets since its annual magnitude is usually much lower than that of fossil fuel emissions within the boundaries of cities. However, human and livestock respiration may be substantial compared to fossil fuel emissions in areas with high population density such as Manhattan or Beijing....

Additional file 10: Table S7. Mutation site information screened by whole genome sequencing.

Additional file 2: Fig. S2. The Sanger sequencing results of the mutation sites of the 15 mutant individual plants, in which the results of SNP-5 and SNP-6 are different from the targeted sequencing results, these two mutant individual plants are excluded, and the rest are the same as the targeted sequencing results, which are true variation.

Additional file 5: Table S2. Primers for amplifying fragments of the target site.

Additional file 9: Table S6. Mutation site information obtained by targeted sequencing.

Additional file 12: Fig. S5. Enrichment analysis of DEGs for B. gargarizans metamorphosis. The top 30 GO terms and pathways of significant enrichment for the (A and B) upregulated DEGs and (C and D) downregulated DEGs. The y-axis corresponds to the GO term or KEGG pathway, the x-axis corresponds to the EnrichmentScore, and the size of the point corresponds to the number of differential genes in the term or pathway.

Additional file 8: Supplementary Fig. S5. Plasma IL-8 has no relationship with TNM stage and lymph node metastasis in ESCC patients. The expression levels of IL-8 in the plasma of ESCC patients were detected by ELISA assay and analyzed with TNM stage (A) and lymph node metastasis (B). ns, not significant.

Abstract Background Esophageal carcinoma is the highly lethal cancer in the world, predominantly in some areas of East Asia. We previously reported that overexpression of cytoskeleton regulator Wiskott-Aldrich syndrome protein and SCAR Homolog (WASH) associates with poor prognosis of patients with esophageal squamous cell carcinoma (ESCC). However, the molecular mechanism and clinical significance involved in WASH overexpression have not been fully elucidated. Methods Bioinformatics analysis and luciferase reporter assay were used to predict and validate...

Additional file 7: Fig. S7. Only a few proteins and transcripts have potential impacts on TMA production. A Ranked abundance plot of proteins and transcripts in duck livers. The top 10 most abundant proteins accounted for 12.7% of the total proteins, and the top 10 most abundant transcripts covered 33.8% of all transcripts in this tissue. B Changes in protein and transcript abundances. The upper-right panel shows the proportion of significantly altered proteins and transcripts.

Additional file 9: Table S2. Differently expressed protein applying TMT10plex.

Abstract Background Most duck eggs possess a fishy odor, indicating that ducks generally exhibit impaired trimethylamine (TMA) metabolism. TMA accumulation is responsible for this unpleasant odor, and TMA metabolism plays an essential role in trimethylaminuria (TMAU), also known as fish odor syndrome. In this study, we focused on the unusual TMA metabolism mechanism in ducks, and further explored the unclear reasons leading to the debilitating TMA metabolism. Methods To achieve this, transcriptome, proteome, and metagenome...

Additional file 1: Table S1. Inclusionand exclusion criteria for subjects enrollment. Table S2. Characteristics of the validationcohort. Table S3. Relative abundance of 23 altered genera identified in SPgroup. Table S4. Relative abundance of 50 altered genera identified in AEgroup. Table S5. Significantly variant metabolicpathways identified in each group using LEfSe analyses. TableS6. LDA score values of 23 differential genera. Table S7. Eighthundred and thirty-five metabolites identified both from HC and SP group. Fig. S1. Correlationsbetween...

Additional file 1: Table S1. Differentially expressed proteins identified in plasma samples.

Additional file 2. Table S2: Analysis of the physical properties of CNX genes in G. hirsutum.

Additional file 5. Table S5: CNX family members cis-acting elements and their locations.

Additional file 6. Table S6: Relative expression levels of CNX family genes after four different abiotic stress treatments.

Additional file 6. Table S6: Relative expression levels of CNX family genes after four different abiotic stress treatments.

Additional file 6: Figure S6. Single-cell sequencing analysis of adult mice intestine. a Dotplot for highest specificity gene markers of cell clusters. Dot size represents the percentage of cells expressing the denoted gene, and the color represents average normalized expression level within the denoted cluster. b The expression of GFAP in different cell clusters by analysis of the Uniform Manifold Approximation and Projection (UMAP). c Different cell subpopulation of the GSE156905 dataset derived from the...

Abstract Background Clear aligner (CA) treatment has been gaining popularity, but the biomechanical effects of CAs in bimaxillary dentition have not been thoroughly investigated. Direct and indirect strong anchorages are two common anchorage control methods, but the underlying biomechanical mechanism has not yet been elucidated. This study aimed to investigate the different biomechanical effects of CAs in closing the bimaxillary space under different anchorage controls, further instructing the compensation strategies design and strong anchorage choice...

Additional file 1: Appendix Outline.

Additional file 1: Appendix Outline.

Additional file 3: Appendix 2. Searching Strategy.