2 Works

Data from: A comparison between transcriptome sequencing and 16S metagenomics for detection of bacterial pathogens in wildlife

Maria Razzauti, Maxime Galan, Maria Bernard, Sarah Maman, Christophe Klopp, Nathalie Charbonnel, Muriel Vayssier-Taussat, Marc Eloit & Jean-François Cosson
Background: Rodents are major reservoirs of pathogens responsible for numerous zoonotic diseases in humans and livestock. Assessing their microbial diversity at both the individual and population level is crucial for monitoring endemic infections and revealing microbial association patterns within reservoirs. Recently, NGS approaches have been employed to characterize microbial communities of different ecosystems. Yet, their relative efficacy has not been assessed. Here, we compared two NGS approaches, RNA-Sequencing (RNA-Seq) and 16S-metagenomics, assessing their ability to...

Data from: Assessment of a 16S rRNA amplicon Illumina sequencing procedure for studying the microbiome of a symbiont-rich aphid genus

Emmanuelle Jousselin, Anne-Laure Clamens, Maxime Galan, Maria Bernard, Sarah Maman, Bernhard Gschloessl, Marie-Gabrielle Duport, Andrea S. Meseguer, Federica Calevro, Armelle Coeur D'acier, G. Duport & A.-L. Clamens
The bacterial communities inhabiting arthropods are generally dominated by a few endosymbionts that play an important role in the ecology of their hosts. Rather than comparing bacterial species richness across samples, ecological studies on arthropod endosymbionts often seek to identify the main bacterial strains associated with each specimen studied. The filtering out of contaminants from the results and the accurate taxonomic assignment of sequences are therefore crucial in arthropod microbiome studies. We aimed here to...

Registration Year

  • 2015
    2

Resource Types

  • Dataset
    2

Affiliations

  • Genetique Animale et Biologie Integrative
    2
  • Montpellier SupAgro
    2
  • Génétique Physiologie et Systèmes d'Elevage
    2
  • University of Lyon System
    1
  • French National Institute for Agricultural Research
    1
  • French Agency for Food, Environmental and Occupational Health & Safety
    1
  • Institut Pasteur
    1
  • Institut National des Sciences Appliquées de Lyon
    1