5,552 Works

Functional redundancy changes along a drought stress gradient for the shift of selection effect to complementarity effect in experimental plant communities

Song Gao, Xiaoyan Wang, Tong Chen, Qiaodi Yan, Mengchun Shen, Jiang Wang & Feihai Yu
Species interactions alter with environmental change, which influences the relationship between biodiversity and ecosystem functioning (BEF). How BEF relationships change with environments requires knowledge of the changes of functional attributes in individual species in response to environmental change. Here, we assembled experimental plant communities with different species richness (1, 2, 4, 8, and 16) and exposed them to a drought stress gradient (control, moderate and intensive drought stress). Functional redundancy decreased with increasing drought stress...

KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity

Guokai Yan, Xiuzhi Li, Zilong Zheng, Weihua Gao, Changqing Chen, Xinkai Wang, Zhongyi Cheng, Jie Yu, Geng Zou, Muhammad Zahid Farooq, Xiaoyan Zhu, Weiyun Zhu, Qing Zhong & Xianghua Yan
Amino acids play crucial roles in the MTOR (mechanistic target of rapamycin kinase) complex 1 (MTORC1) pathway. However, the underlying mechanisms are not fully understood. Here, we establish a cell-free system to mimic the activation of MTORC1, by which we identify CANX (calnexin) as an essential regulator for leucine-stimulated MTORC1 pathway. CANX translocates to lysosomes after leucine deprivation, and its loss of function renders either the MTORC1 activity or the lysosomal translocation of MTOR insensitive...

ATG7-mediated autophagy facilitates embryonic stem cell exit from naive pluripotency and marks commitment to differentiation

Jilong Zhou, Hainan He, Jing-Jing Zhang, Xin Liu, Wang Yao, Chengyu Li, Tian Xu, Shu-Yuan Yin, Dan-Ya Wu, Cheng-Li Dou, Qiao Li, Jiani Xiang, Wen-Jing Xiong, Li-Yan Wang, Jun-ming Tang, Zhouyiyuan Xue, Xia Zhang & Yi-Liang Miao
Macroautophagy/autophagy is a conserved cellular mechanism to degrade unneeded cytoplasmic proteins and organelles to recycle their components, and it is critical for embryonic stem cell (ESC) self-renewal and somatic cell reprogramming. Whereas autophagy is essential for early development of embryos, no information exists regarding its functions during the transition from naive-to-primed pluripotency. Here, by using an in vitro transition model of ESCs to epiblast-like cells (EpiLCs), we find that dynamic changes in ATG7-dependent autophagy are...

Reproducibility of the Infinium methylationEPIC BeadChip assay using low DNA amounts

Steffan Noe Christiansen, Jeppe Dyrberg Andersen, Marie-Louise Kampmann, Jing Liu, Mikkel Meyer Andersen, Jacob Tfelt-Hansen & Niels Morling
The Infinium MethylationEPIC BeadChip (EPIC) is a reliable method for measuring the DNA methylation of more than 850,000 CpG positions. In clinical and forensic settings, it is critical to be able to work with low DNA amounts without risking reduced reproducibility. We evaluated the EPIC for a range of DNA amounts using two-fold serial dilutions investigated on two different days. While the β-value distributions were generally unaffected by decreasing DNA amounts, the median squared Pearson’s...

Study and numerical analysis of Von Mises stress of a new tumor-type distal femoral prosthesis comprising a peek composite reinforced with carbon fibers: finite element analysis

Yu Guo & Wei Guo
Research on carbon-fiber-reinforced polyetheretherketone (CF-PEEK/CFR-PEEK) as a bone tumor joint prosthesis remains limited. Herein we numerically determine the feasibility of CF-PEEK material containing 30% Wt carbon fiber (CF30-PEEK) as a material for the dual-action tumor-type distal femoral prosthesis. Use CT scan method to build a complete finite element model of the knee joint. Simulate the resection of the distal femoral tumor, and then reconstruct it with the dual-action tumor-type distal femoral prosthesis. The femoral condyle...

Cenozoic rifting and inversion of Beibuwan Basin and its linkage with the strike-slip movement along the Ailao Shan-Red River Shear Zone

Yanjun Cheng, Zhiping Wu, Jie Zhang, Yuqing Liu, Zhengkai Wang & Yining Dai
The Cenozoic Beibuwan Basin is located along the eastern margin of the Ailao Shan Red River Shear Zone (ASRRSZ). The rifting and inversion processes of this basin provide important clues to the mechanism by which extrusion tectonics drive continental rifting and inversion development. However, the rifting and inversion of this basin remain ambiguous. Here, we employ seismic data, information about the time and displacement of faulting, syn-rift fault and isopach maps to analyse the Cenozoic...

One novel naphthalene derivative and other constituents with anti-complementary activities from the aerial parts of Dracocephalum moldavica

Jia-Ming Wang, Jin-Feng Sun, Long Jin, Meng-Jie Wang, Yan-Yan Huang, Mei Jin, Wei Zhou & Gao Li
One novel naphthalene derivative, 2-octa-2′,4′,6′-atriynenaphthalene (1), together with eighteen known compounds (2–19) were isolated from the aerial parts of Dracocephalum moldavica L. Compounds 2, 8, 10, 13, 15–17 and 19 were obtained from the family Lamiaceae for the first time, and compounds 11 and 18 were firstly identified from the genus of Dracocephalum. All the isolates were evaluated for anti-complementary activities through the classical and alternative pathways, and the targets of the most active compounds...

A transcriptomic analysis of neuropathic pain in the anterior cingulate cortex after nerve injury

Yu Zhang, Shiwei Jiang, Fei Liao, Zhifeng Huang, Xin Yang, Yu Zou, Xin He, Qulian Guo & Changsheng Huang
The anterior cingulate cortex (ACC) is a core brain region processing pain emotion. In this study, we performed RNA sequencing analysis to reveal transcriptomic profiles of the ACC in a rat chronic constriction injury (CCI) model. A total of 1628 differentially expressed genes (DEGs) were identified by comparing sham-operated rats with rats of 12 hours, 1, 3, 7, and 14 days after surgery, respectively. Although these inflammatory-related DEGs were generally increased after CCI, different kinetics...

Melatonin affects hypoxia-inducible factor 1α and ameliorates delayed brain injury following subarachnoid hemorrhage via H19/miR-675/HIF1A/TLR4

Zhijian Xu, Fengduo Zhang, Hu Xu, Fan Yang, Gezhi Zhou, Minfeng Tong, Yaqiang Li & Song Yang
This study aimed to investigate the molecular mechanism of how melatonin (MT) interferes with hypoxia-inducible factor 1α (HIF1A) and toll-like receptor 4 (TLR4) expression, which is implicated in the management of delayed brain injury (DBI) after subarachnoid hemorrhage (SAH). Luciferase assay, real-time PCR, Western-blot analysis and immunohistochemistry (IHC) assays were utilized to explore the interaction among H19, miR-675, HIF1A and TLR4, and to evaluate the effect of MT on the expression of above transcripts in...

Physicochemical properties, and volatile compounds of blackened jujube vinegar as prepared by optimized fermentation process

Fangzhou Wang, Yaru Song, Sriram K. Vidyarthi & Rentang Zhang
Response surface methodology was used to optimize the fermentation factors of blackened jujube vinegar (BJV). Physicochemical properties, antioxidant activity, sensory evaluation, and volatile compounds were studied with the methods of high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). The results indicated that the optimal fermentation conditions was the acetic acid inoculation amount of 15%, the fermentation temperature of 30.44°C, and the initial alcohol content of 6.45% vol. During fermentation processing, there was a delay...

Effects of glycerol and glucose on docosahexaenoic acid synthesis in Aurantiochyrium limacinum SFD-1502 by transcriptome analysis

Huaqiu Zhang, Xiangying Zhao, Chen Zhao, Jiaxiang Zhang, Yang Liu, Mingjing Yao & Jianjun Liu
Docosahexaenoic acid (DHA) has numerous functions in adjusting the organic health and pragmatic value in medicine and food field. In this study, we compared glycerol and glucose as the only carbon source for DHA production by Aurantiochytrium. When the glycerol concentration was 120 g/L, the maximum DHA yield was 11.08 g/L, and the DHA yield increased significantly, reaching 47.67% of the total lipid content. When the cells grew in glucose, the DHA proportion was 37.39%....

Water-soluble chitosan and phytoremediation efficiency of two Brassica napus L. cultivars in cadmium-contaminated farmland soils

Jian-Lin Bian, Wei Cao, Jun-Mei Guo, Jun-Xing Yang, Xue-Dong Wang, Jie Wang, Jun Huang, Tian-Xiang Xia & Cun-Yan Xia
Pot and field trials were conducted to investigate Cd uptake and phytoremediation efficiency of two Brassica napus cultivars (QY-1 and SYH) with applied water-soluble chitosan (WSC, Pot: 0, 2% and 4%; Field: 0 and 10 g·m−2) grown in Cd-contaminated soils. The results from the pot and field trials generally showed that WSC treatments significantly increased Cd concentrations in shoot and root tissues by 33.77–159.71% (except for SYH/JY) and 7.42–168.71% of two B. napus cultivars compared...

ATG7-mediated autophagy facilitates embryonic stem cell exit from naive pluripotency and marks commitment to differentiation

Jilong Zhou, Hainan He, Jing-Jing Zhang, Xin Liu, Wang Yao, Chengyu Li, Tian Xu, Shu-Yuan Yin, Dan-Ya Wu, Cheng-Li Dou, Qiao Li, Jiani Xiang, Wen-Jing Xiong, Li-Yan Wang, Jun-Ming Tang, Zhouyiyuan Xue, Xia Zhang & Yi-Liang Miao
Macroautophagy/autophagy is a conserved cellular mechanism to degrade unneeded cytoplasmic proteins and organelles to recycle their components, and it is critical for embryonic stem cell (ESC) self-renewal and somatic cell reprogramming. Whereas autophagy is essential for early development of embryos, no information exists regarding its functions during the transition from naive-to-primed pluripotency. Here, by using an in vitro transition model of ESCs to epiblast-like cells (EpiLCs), we find that dynamic changes in ATG7-dependent autophagy are...

Selective autophagic degradation of ACLY (ATP citrate lyase) maintains citrate homeostasis and promotes oocyte maturation

Hainan He, Junling Wang, Xingmei Mou, Xin Liu, Qiao Li, Mingyue Zhong, Bingbing Luo, Zhisheng Yu, Jingjing Zhang, Tian Xu, Chengli Dou, Danya Wu, Wei Qing, Linhui Wu, Kai Zhou, Zhengang Fan, Tingting Wang, Taotao Hu, Xia Zhang, Jilong Zhou & Yi-Liang Miao
Macroautophagy/autophagy is a cellular and energy homeostatic mechanism that contributes to maintain the number of primordial follicles, germ cell survival, and anti-ovarian aging. However, it remains unknown whether autophagy in granulosa cells affects oocyte maturation. Here, we show a clear tendency of reduced autophagy level in human granulosa cells from women of advanced maternal age, implying a potential negative correlation between autophagy levels and oocyte quality. We therefore established a co-culture system and show that...

ATF3 -activated accelerating effect of LINC00941/lncIAPF on fibroblast-to-myofibroblast differentiation by blocking autophagy depending on ELAVL1/HuR in pulmonary fibrosis

Jinjin Zhang, Haixia Wang, Hongbin Chen, Hongbo Li, Pan Xu, Bo Liu, Qian Zhang, Changjun Lv & Xiaodong Song
Idiopathic pulmonary fibrosis (IPF) is characterized by lung scarring and has no effective treatment. Fibroblast-to-myofibroblast differentiation and myofibroblast proliferation and migration are major clinical manifestations of this disease; hence, blocking these processes is a practical treatment strategy. Here, highly upregulated LINC00941/lncIAPF was found to accelerate pulmonary fibrosis by promoting fibroblast-to-myofibroblast differentiation and myofibroblast proliferation and migration. Assay for transposase-accessible chromatin using sequencing and chromatin immunoprecipitation experiments elucidated that histone 3 lysine 27 acetylation (H3K27ac) activated...

Tubular cells produce FGF2 via autophagy after acute kidney injury leading to fibroblast activation and renal fibrosis

Man J. Livingston, Shaoqun Shu, Ying Fan, Ze Li, Qiong Jiao, Xiao-Ming Yin, Manjeri A. Venkatachalam & Zheng Dong
Following acute kidney injury (AKI), renal tubular cells may stimulate fibroblasts in a paracrine fashion leading to interstitial fibrosis, but the paracrine factors and their regulation under this condition remain elusive. Here we identify a macroautophagy/autophagy-dependent FGF2 (fibroblast growth factor 2) production in tubular cells. Upon induction, FGF2 acts as a key paracrine factor to activate fibroblasts for renal fibrosis. After ischemic AKI in mice, autophagy activation persisted for weeks in renal tubular cells. In...

The Effect of the Acidic Gas Pollutant on Wool Keratin Fiber Structure

Zhe Jiang, Rubing Bai, Jinfang Zhang, Ende Li, Yifan Cui, Ning Zhang, Nan Zhang & Qiang Wang
Increase in air pollution caused by acidic gases, such as nitrogen dioxide, call for the need to study potential effects on the structure of textile materials. Wool is a natural protein fiber which is easily damaged due to rich reaction positions and cross-linked bonds in the fiber. This study reports, for the first time, effect of nitrogen dioxide, as a model acid gas pollutant, on the structure of wool. On the macro level, wool fabric...

Protein disulfide isomerases (PDIs) negatively regulate ebolavirus structural glycoprotein expression in the endoplasmic reticulum (ER) via the autophagy-lysosomal pathway

Bin Wang, Jing Zhang, Xin Liu, Qingqing Chai, Xiaoran Lu, Xiaoyu Yao, Zhichang Yang, Liangliang Sun, Silas F. Johnson, Richard C Schwartz & Yong-Hui Zheng
Zaire ebolavirus (EBOV) causes a severe hemorrhagic fever in humans and non-human primates with high morbidity and mortality. EBOV infection is dependent on its structural glycoprotein (GP), but high levels of GP expression also trigger cell rounding, detachment, and downregulation of many surface molecules that is thought to contribute to its high pathogenicity. Thus, EBOV has evolved an RNA editing mechanism to reduce its GP expression and increase its fitness. We now report that the...

LINC00467 facilitates the proliferation, migration and invasion of glioma via promoting the expression of inositol hexakisphosphate kinase 2 by binding to miR-339-3p

Yin Zhang, Yaxuan Zhang, Sen Wang, Boqiang Cao, Daling Hu, Junli Jia, Yuhang Wang, Luyao Chen, Jiaming Li, Hongyi Liu & Huamin Tang
Our previous studies indicate that long noncoding RNA (lncRNA) LINC00467 can act as an oncogene to participate in the malignant progression of glioma, but the underlying molecular mechanism remains to be studied further. This study aimed to explore the biological role of the LINC00467/miR-339-3p/ inositol hexakisphosphate kinase 2 (IP6K2) regulatory axis in glioma. The Cancer Genome Atlas (TCGA), Oncomine databases and reverse transcription‑quantitative PCR (RT‑qPCR) were used to analyze IP6K2 expression in glioma. RT-PCR, EdU...

Acetylshikonin suppresses diffuse large B-Cell Lymphoma cell growth by targeting the T-lymphokine-activated killer cell-originated protein kinase signalling pathway

Jieke Cui, Rong Guo, Yingjun Wang, Yue Song, Xuewen Song, Hongwen Li, Xiaoqin Song & Jiwei Li
Diffuse large B-cell lymphoma (DLBCL) is one of the most common causes of cancer death worldwide, and responds poorly to the existing treatments. Thus, identifying novel therapeutic targets of DLBCL is urgently needed. In this study, we found that T-lymphokine-activated killer cell-originated protein kinase (TOPK) was highly expressed in DLBCL cells and tissues. Data from the GEPIA database also indicated that TOPK was highly expressed in DLBCL tissues. The high expression levels of proteins were...

Mechanism of bluish pigment formation in lotus rhizome starch with ferrous sulfate and its application in rapid detection of adulteration

Jie Wang, Yanzhao Liu, Jie Li, Ying Diao, Zhongli Hu & Shoulei Yan
Lotus rhizome starch (LRS) is an important traditional processed product of lotus rhizome in China due to its good taste and high nutritional value. However, adulterated lotus rhizome starches are prevalent on the market. In this study, a novel method for the rapid detection of adulteration in LRS based on color reaction between ferrous sulfate (FS) and (-)-gallocatechin (GC) in LRS was studied. The polyphenols in LRS were analyzed by liquid chromatography-mass spectrometry (LC-MS/MS) as...

Genetic and pathogenic variation of Botrytis cinerea, the causal agent of grey mould on Panax ginseng in China

Rong Wang, Kun Liu, Bingwei Chen, Wanlong Ding & Yong Li
Grey mould, caused by Botrytis cinerea, is an increasingly destructive disease of ginseng (Panax ginseng) in China. However, the extent of variation in the morphological characteristics of fungal colonies, genetic background and pathogenicity of different strains of this pathogen remains unknown. In this study, we isolated and identified 102 isolates of B. cinerea from ginseng. Among these, the sclerotial phenotype was dominant, followed by the mycelial and conidial phenotypes, with the proportion of each phenotype...

Anatexis of late Neoarchean-Early Paleoproterozoic and late Paleoproterozoic garnet-bearing leucogranite from the Khondalite Belt in the North China Craton

Qiang Shi, Zhong-Yuan Xu, Guo-Chun Zhao, Ding Ding, Guang-Wei Liu, Zhong-Hai Zhao, Jing-Yu Yu, Chen Li, Li-Qiang Bai, Yao Wang, Bing Guo, Kai Zhang & Chun-Feng Liu
Two episodes of late Neoarchean-early Paleoproterozoic and late Paleoproterozoic garnet-bearing leucogranite, named the Baotou and Jining–Liangcheng garnet-bearing leucogranite are recognized from the Khondalite Belt in the North China Craton. Geochemical studies show that garnet-bearing leucogranite has high Al2O3 content and FeOT/MgO ratio, with a large variation in the CaO content and K2O/Na2O ratio. Additionally, the garnet-bearing leucogranite is enriched in light rare earth elements and large ion lithophile elements and depleted in Nb, Ta, P,...

Diversity of nirS and nirK denitrifying bacteria in rhizosphere and non-rhizosphere soils of halophytes in Ebinur Lake Wetland

Yanhui Niu, Wenge Hu, Tingting Zhou, Bo He, Xuemei Chen & Yang Li
There are few studies on denitrification related to the nitrogen cycle in Ebinur Lake wetland. This study aimed to explore the response of the diversity and composition of denitrifying bacteria to the environmental factors in wetland, so as to obtain more information about the community structure of denitrifying bacteria driven by environmental factors. Using the genes encoding nitrite reductase (nirS and nirK) as molecular markers, we analysed the seasonal changes in the diversity of denitrifying...

Additional file 2 of A CT-based nomogram for predicting the risk of adenocarcinomas in patients with subsolid nodule according to the 2021 WHO classification

Qilong Song, Biao Song, Xiaohu Li, Bin Wang, Yuan Li, Wu Chen, Zhaohua Wang, Xu Wang, Yongqiang Yu, Xuhong Min & Dongchun Ma
Additional file 2: Supplemental Table 2. The kappa coefficients of categorical variables between three radiologists.

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  • Chinese PLA General Hospital
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Shanghai Jiao Tong University
  • Fudan University
  • Zhejiang University
  • Capital Medical University
  • Sichuan University
  • Sun Yat-sen University
  • Huazhong University of Science and Technology
  • Chinese Academy of Sciences