A high-throughput genetic screening platform in a single-celled photosynthetic eukaryote would be a transformative addition to the plant biology toolbox. Here, we present ChlaMmeSeq (Chlamydomonas MmeI-based insertion site Sequencing), a tool for simultaneous mapping of tens of thousands of mutagenic insertion sites in the eukaryotic unicellular green alga Chlamydomonas reinhardtii. We first validated ChlaMmeSeq by in-depth characterization of individual insertion sites. We then applied ChlaMmeSeq to a mutant pool and mapped 11,478 insertions, covering 39%...

Fire is a key determinant of woody vegetation structure in savanna ecosystems, acting both independently and synergistically through interactions with herbivores. Fire influences biodiversity and ecological functioning, but quantifying its effects on woody structure is challenging at both species and community scales. Deeper insight into fire effects, and fire-herbivore interactions, can be gained through the examination of species-specific demographic and dynamic changes occurring across areas with different fire regimes in the presence of large herbivores....