24 Works

Macrophage targeting of nitazoxanide-loaded transethosomal gel in cutaneous leishmaniasis

Husna Khalid, Sibgha Batool, Fakhar Ud-Din, Salman Khan & Gul Khan
Topical delivery is preferable over systemic delivery for cutaneous leishmaniasis (CL), because of its easy administration, reduced systemic adverse effects, and low cost. Nitazoxanide (NTZ) has broad-spectrum activity against various parasites and has the potential to avoid drug resistance developed by enzymatic mutations. NTZ oral formulation is being associated with severe dyspepsia and stomach pain. Herein, NTZ-transethosomes (NTZ-TES) were prepared and loaded into chitosan gel (NTZ-TEG) for topical delivery. NTZ-TES were prepared by thin-film-hydration method...

Glaciochemistry and Pigment Producing Ability of Bacteria from the Roof of the World, the Glaciers of Karakoram, Pakistan

Noor Hassan, Muhammad Rafiq, Abdul Haleem, Naveed Iqbal, Mohsin Khan, Aamir Ali Shah & Fariha Hasan
The Karakoram Mountain Range (KMR) is one of the largest mountain ranges in the world, with ∼37% of its area glaciated. Glaciers harbor various microbes, including bacteria exposed to various extreme conditions. To thrive under these conditions, bacteria adopt various strategies, one of the most important being the production of pigments. Here, we present the geochemistry of ice, sediment and meltwaters sampled from Ghulmet, Ghulkin and Hopar glaciers of the Karakoram Range, Pakistan. In addition,...

New lead compounds identification against KRas mediated cancers through pharmacophore-based virtual screening and in vitro assays

Mehreen Ghufran, Ashfaq Ur Rehman, Muhammad Ayaz, Zaheer ul-Haq, Reaz Uddin, Syed Sikander Azam & Abdul Wadood
Cancer remains the leading cause of mortality and morbidity in the world, with 19.3 million new diagnoses and 10.1 million deaths in 2020. Cancer is caused due to mutations in proto-oncogenes and tumor-suppressor genes. Genetic analyses found that Ras (Rat sarcoma) is one of the most deregulated oncogenes in human cancers. The Ras oncogene family members including NRas (Neuroblastoma ras viral oncogene homolog), HRas (Harvey rat sarcoma) and KRas are involved in different types of...

Comparison of the safety and immunogenicity of the BNT-162b2 vaccine and the ChAdOx1 vaccine for solid organ transplant recipients: a prospective study

Aziza A. Ajlan, Tariq Ali, Hassan Aleid, Khalid Almeshari, Edward DeVol, Morad Ahmed Alkaff, Layal Fajji, Ali Alali, Dani Halabi, Sahar Althuwaidi, Saad Alghamdi, Asad Ullah, Abdulrahman Alrajhi, Khalid Bzeizi, Reem Almaghrabi, Kris Ann Hervera Marquez, Bilal Elmikkaoui, Eid Albogumi, Haifa Aldakhil, Moheeb Al-Awwami & Dieter C. Broering
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and its resulting disease, coronavirus disease 2019 (COVID-19), has spread to millions of people worldwide. Preliminary data from organ transplant recipients have shown reduced seroconversion rates after the administration of different SARS-CoV-2 vaccination platforms. However, it is unknown whether different vaccination platforms provide different levels of protection against SARS-CoV-2. To answer this question, we prospectively studied 431 kidney and liver transplant recipients (kidney: n = 230;...

Comparative analysis of mRNA and inactivated COVID-19 vaccines: A study from Faisalabad district of Pakistan

Syed Ata Ul Munamm, Iftikhar Nadeem, Noor Mahdi, Muhammad Saqlain, Zaid Khalid Rana, Usman Feroze Khatana, Umer Mustansir Bhatty, Visakan Navayogaarajah, Fatimah Mahsal Khan & Masood Ur Rasool
Background & aims:Pakistan has vaccinated over 122 million people. The two vaccines in widespread use are inactivated (BBIBP-CorV & Sinovac) and mRNA forms (BNT162b2 & mRNA-1273). The primary aim of this study was to compare these two forms of vaccines against unvaccinated individuals collectively and then to see which one is more efficacious.Methods:Case–control study design was used to compare the efficacy of inactivated and mRNA vaccines against symptomatic infection, hospitalisations and mortality due to Severe...

sj-doc-1-rcp-10.1177_14782715221131409 – Supplemental material for Comparative analysis of mRNA and inactivated COVID-19 vaccines: A study from Faisalabad district of Pakistan

Syed Ata Ul Munamm, Iftikhar Nadeem, Noor Mahdi, Muhammad Saqlain, Zaid Khalid Rana, Usman Feroze Khatana, Umer Mustansir Bhatty, Visakan Navayogaarajah, Fatimah Mahsal Khan & Masood Ur Rasool
Supplemental material, sj-doc-1-rcp-10.1177_14782715221131409 for Comparative analysis of mRNA and inactivated COVID-19 vaccines: A study from Faisalabad district of Pakistan by Syed Ata Ul Munamm, Iftikhar Nadeem, Noor Mahdi, Muhammad Saqlain, Zaid Khalid Rana, Usman Feroze Khatana, Umer Mustansir Bhatty, Visakan Navayogaarajah, Fatimah Mahsal Khan and Masood Ur Rasool in Journal of the Royal College of Physicians of Edinburgh

Comparison of the safety and immunogenicity of the BNT-162b2 vaccine and the ChAdOx1 vaccine for solid organ transplant recipients: a prospective study

Aziza A. Ajlan, Tariq Ali, Hassan Aleid, Khalid Almeshari, Edward DeVol, Morad Ahmed Alkaff, Layal Fajji, Ali Alali, Dani Halabi, Sahar Althuwaidi, Saad Alghamdi, Asad Ullah, Abdulrahman Alrajhi, Khalid Bzeizi, Reem Almaghrabi, Kris Ann Hervera Marquez, Bilal Elmikkaoui, Eid Albogumi, Haifa Aldakhil, Moheeb Al-Awwami & Dieter C. Broering
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and its resulting disease, coronavirus disease 2019 (COVID-19), has spread to millions of people worldwide. Preliminary data from organ transplant recipients have shown reduced seroconversion rates after the administration of different SARS-CoV-2 vaccination platforms. However, it is unknown whether different vaccination platforms provide different levels of protection against SARS-CoV-2. To answer this question, we prospectively studied 431 kidney and liver transplant recipients (kidney: n = 230;...

Pharmacological evaluation of newly synthesized benzimidazole derivative for anti-Alzheimer potential

Aleeza Ahmed, Arif-ullah Khan, Humaira Nadeem, Muhammad Imran & Nadeem Irshad
Backgound: Alzheimer disease (AD) is a disastrous disease characterized by accretion of amyloid-beta plaques, neurofibrillary tangles inducing oxidative stress, loss of neuronal functions and continuous progression of cognitive impairment leading to severe dementia. Material and Methods: The newly synthesized benzimidazole derivative 4-chloro-3-(2-phenyl-1H-benzimidazole-1-sulfonyl) benzoic acid (CB) was evaluated for its anti-Alzheimer activity using in silico, in vivo, in vitro and molecular techniques (ELISA, WB & IHC). Results:In-silico studies revealed that CB has atomic contact energy values...

Pharmacological evaluation of newly synthesized benzimidazole derivative for anti-Alzheimer potential

Aleeza Ahmed, Arif-ullah Khan, Humaira Nadeem, Muhammad Imran & Nadeem Irshad
Backgound: Alzheimer disease (AD) is a disastrous disease characterized by accretion of amyloid-beta plaques, neurofibrillary tangles inducing oxidative stress, loss of neuronal functions and continuous progression of cognitive impairment leading to severe dementia. Material and Methods: The newly synthesized benzimidazole derivative 4-chloro-3-(2-phenyl-1H-benzimidazole-1-sulfonyl) benzoic acid (CB) was evaluated for its anti-Alzheimer activity using in silico, in vivo, in vitro and molecular techniques (ELISA, WB & IHC). Results:In-silico studies revealed that CB has atomic contact energy values...

KIF1A novel frameshift variant p.(Ser887Profs*64) exhibits clinical heterogeneity in a Pakistani family with hereditary sensory and autonomic neuropathy type IIC

Saima Ghafoor, Muhammad Arshad Rafiq, Syed Tahir Abbas Shah, Muhammad Ansar, Tara Paton, Muhmmad Ajmal, Zehra Agha, Raheel Qamar & Maleeha Azam
Background: Hereditary sensory and autonomic neuropathies (HSANs) are rare heterogeneous group of neurological disorders caused by peripheral nerve deterioration. The HSANs sub-clinical classes have clinical and genetic overlap which often lead to misdiagnosis. In the present study a Pakistani family with five affected members suffering from severe neuropathy were genetically analyzed to identify the disease causative element in the family. Methods: Genome wide high-density single nucleotide polymorphism (SNP) microarray analysis was carried out followed by...

A new nonsense mutation in HMX1 in two siblings with oculoauricular syndrome

Muhammad Ansar, Samra Javed, Hafiz Muhammad Azhar Baig, Mathieu Quinodoz, Mukhtar Ullah, Ji Hoon Han, Muhammad Usama Rahim, Humera Kausar, Giacomo Calzetti & Carlo Rivolta
A new nonsense mutation in HMX1 in two siblings with oculoauricular syndrome

Data from: Genomic analysis of codon usage shows influence of mutation pressure, natural selection, and host features on Marburg virus evolution

Izza Nasrullah, Azeem M Butt, Shifa Tahir, Muhammad Idrees & Yigang Tong
Background. The Marburg virus (MARV) has a negative-sense single-stranded RNA genome, belongs to the family Filoviridae, and is responsible for several outbreaks of highly fatal hemorrhagic fever. Codon usage patterns of viruses reflect a series of evolutionary changes that enable viruses to shape their survival rates and fitness toward the external environment and, most importantly, their hosts. To understand the evolution of MARV at the codon level, we report a comprehensive analysis of synonymous codon...

Diet of the brown bear in Himalaya: combining classical and molecular genetic techniques

Muhammad Ali Nawaz, Alice Valentini, Noor Kamal Khan, Christian Miquel, Pierre Taberlet & Jon E. Swenson
The ecological requirements of brown bears are poorly known in the Himalaya region, which complicates conservation efforts. We documented the diet of the Himalayan brown bear ( Ursus arctos isabellinus ) by combining classical scat analysis and a newly developed molecular genetic technique (the trn L approach), in Deosai National Park, Pakistan. Brown bears consumed over 50 plant species, invertebrates, ungulates, and several rodents. Eight plant families; Poaceae, Polygonaceae, Cyperaceae, Apiaceae, Asteraceae, Caryophyllaceae, Lamiaceae, and...

RNA-seq based expression of DEGs in roots and leaves of bread wheat

Saman Maqbool & Awais Rasheed
RNA from roots and leaves of twenty eight bread wheat genotypes was extracted, purified, quantified and sent to Beijing Genomics Institute (BGI) for sequencing. Briefly, mRNAs were isolated and fragmented from total RNA using the oligo (dT) method for cDNA synthesis. The 75-bp single-end sequencing libraries were constructed, and sequencing was performed on HiSeq X Ten (Illumina, San Diego, USA) using standard protocols. Adapters with unknown bases (N’s > 5%) and low quality were removed...

A new nonsense mutation in HMX1 in two siblings with oculoauricular syndrome

Muhammad Ansar, Samra Javed, Hafiz Muhammad Azhar Baig, Mathieu Quinodoz, Mukhtar Ullah, Ji Hoon Han, Muhammad Usama Rahim, Humera Kausar, Giacomo Calzetti & Carlo Rivolta
A new nonsense mutation in HMX1 in two siblings with oculoauricular syndrome

KIF1A novel frameshift variant p.(Ser887Profs*64) exhibits clinical heterogeneity in a Pakistani family with hereditary sensory and autonomic neuropathy type IIC

Saima Ghafoor, Muhammad Arshad Rafiq, Syed Tahir Abbas Shah, Muhammad Ansar, Tara Paton, Muhmmad Ajmal, Zehra Agha, Raheel Qamar & Maleeha Azam
Background: Hereditary sensory and autonomic neuropathies (HSANs) are rare heterogeneous group of neurological disorders caused by peripheral nerve deterioration. The HSANs sub-clinical classes have clinical and genetic overlap which often lead to misdiagnosis. In the present study a Pakistani family with five affected members suffering from severe neuropathy were genetically analyzed to identify the disease causative element in the family. Methods: Genome wide high-density single nucleotide polymorphism (SNP) microarray analysis was carried out followed by...

Data from: Synthesis and characterization of azo-guanidine based alcoholic media naked eye DNA sensor

Ataf Ali Altaf, Uzma Hashmat, Muhammad Yousaf, Bhajan Lal, Shafiq Ullah, Alvin A. Holder & Amin Badshah
DNA sensing always has an open meadow of curiosity for biotechnologists and other researchers. Recently, in this field, we have introduced an emerging class of molecules containing azo and guanidine functionalities. In this study, we have synthesized three new compounds (UA1, UA6 and UA7) for potential application in DNA sensing in alcoholic medium. The synthesized materials were characterized by elemental analysis, FTIR, UV-visible, 1H NMR and 13C NMR spectroscopies. Their DNA sensing potential were investigated...

Comparative analysis of mRNA and inactivated COVID-19 vaccines: A study from Faisalabad district of Pakistan

Syed Ata Ul Munamm, Iftikhar Nadeem, Noor Mahdi, Muhammad Saqlain, Zaid Khalid Rana, Usman Feroze Khatana, Umer Mustansir Bhatty, Visakan Navayogaarajah, Fatimah Mahsal Khan & Masood Ur Rasool
Background & aims:Pakistan has vaccinated over 122 million people. The two vaccines in widespread use are inactivated (BBIBP-CorV & Sinovac) and mRNA forms (BNT162b2 & mRNA-1273). The primary aim of this study was to compare these two forms of vaccines against unvaccinated individuals collectively and then to see which one is more efficacious.Methods:Case–control study design was used to compare the efficacy of inactivated and mRNA vaccines against symptomatic infection, hospitalisations and mortality due to Severe...

sj-doc-1-rcp-10.1177_14782715221131409 – Supplemental material for Comparative analysis of mRNA and inactivated COVID-19 vaccines: A study from Faisalabad district of Pakistan

Syed Ata Ul Munamm, Iftikhar Nadeem, Noor Mahdi, Muhammad Saqlain, Zaid Khalid Rana, Usman Feroze Khatana, Umer Mustansir Bhatty, Visakan Navayogaarajah, Fatimah Mahsal Khan & Masood Ur Rasool
Supplemental material, sj-doc-1-rcp-10.1177_14782715221131409 for Comparative analysis of mRNA and inactivated COVID-19 vaccines: A study from Faisalabad district of Pakistan by Syed Ata Ul Munamm, Iftikhar Nadeem, Noor Mahdi, Muhammad Saqlain, Zaid Khalid Rana, Usman Feroze Khatana, Umer Mustansir Bhatty, Visakan Navayogaarajah, Fatimah Mahsal Khan and Masood Ur Rasool in Journal of the Royal College of Physicians of Edinburgh

Additional file 1 of Comparison of the safety and immunogenicity of the BNT-162b2 vaccine and the ChAdOx1 vaccine for solid organ transplant recipients: a prospective study

Aziza A. Ajlan, Tariq Ali, Hassan Aleid, Khalid Almeshari, Edward DeVol, Morad Ahmed Alkaff, Layal Fajji, Ali Alali, Dani Halabi, Sahar Althuwaidi, Saad Alghamdi, Asad Ullah, Abdulrahman Alrajhi, Khalid Bzeizi, Reem Almaghrabi, Kris Ann Hervera Marquez, Bilal Elmikkaoui, Eid Albogumi, Haifa Aldakhil, Moheeb Al-Awwami & Dieter C. Broering
Additional file 1: Supplementary Appendix A: Schedule of Assessment.

Additional file 1 of Comparison of the safety and immunogenicity of the BNT-162b2 vaccine and the ChAdOx1 vaccine for solid organ transplant recipients: a prospective study

Aziza A. Ajlan, Tariq Ali, Hassan Aleid, Khalid Almeshari, Edward DeVol, Morad Ahmed Alkaff, Layal Fajji, Ali Alali, Dani Halabi, Sahar Althuwaidi, Saad Alghamdi, Asad Ullah, Abdulrahman Alrajhi, Khalid Bzeizi, Reem Almaghrabi, Kris Ann Hervera Marquez, Bilal Elmikkaoui, Eid Albogumi, Haifa Aldakhil, Moheeb Al-Awwami & Dieter C. Broering
Additional file 1: Supplementary Appendix A: Schedule of Assessment.

New lead compounds identification against KRas mediated cancers through pharmacophore-based virtual screening and in vitro assays

Mehreen Ghufran, Ashfaq Ur Rehman, Muhammad Ayaz, Zaheer ul-Haq, Reaz Uddin, Syed Sikander Azam & Abdul Wadood
Cancer remains the leading cause of mortality and morbidity in the world, with 19.3 million new diagnoses and 10.1 million deaths in 2020. Cancer is caused due to mutations in proto-oncogenes and tumor-suppressor genes. Genetic analyses found that Ras (Rat sarcoma) is one of the most deregulated oncogenes in human cancers. The Ras oncogene family members including NRas (Neuroblastoma ras viral oncogene homolog), HRas (Harvey rat sarcoma) and KRas are involved in different types of...

Glaciochemistry and Pigment Producing Ability of Bacteria from the Roof of the World, the Glaciers of Karakoram, Pakistan

Noor Hassan, Muhammad Rafiq, Abdul Haleem, Naveed Iqbal, Mohsin Khan, Aamir Ali Shah & Fariha Hasan
The Karakoram Mountain Range (KMR) is one of the largest mountain ranges in the world, with ∼37% of its area glaciated. Glaciers harbor various microbes, including bacteria exposed to various extreme conditions. To thrive under these conditions, bacteria adopt various strategies, one of the most important being the production of pigments. Here, we present the geochemistry of ice, sediment and meltwaters sampled from Ghulmet, Ghulkin and Hopar glaciers of the Karakoram Range, Pakistan. In addition,...

Glaciochemistry and Pigment Producing Ability of Bacteria from the Roof of the World, the Glaciers of Karakoram, Pakistan

Noor Hassan, Muhammad Rafiq, Abdul Haleem, Naveed Iqbal, Mohsin Khan, Aamir Ali Shah & Fariha Hasan
The Karakoram Mountain Range (KMR) is one of the largest mountain ranges in the world, with ∼37% of its area glaciated. Glaciers harbor various microbes, including bacteria exposed to various extreme conditions. To thrive under these conditions, bacteria adopt various strategies, one of the most important being the production of pigments. Here, we present the geochemistry of ice, sediment and meltwaters sampled from Ghulmet, Ghulkin and Hopar glaciers of the Karakoram Range, Pakistan. In addition,...

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