Additional file 4: Fig. S4. Phylogeny and community structure of culturable denitrifying bacteria in response to AMF hyphae in the in vitro experiment. A, Phylogenetic tree of culturable denitrifying bacteria from patches of faba bean residue. This was constructed by the neighbor-joining method based on 16S rRNA gene sequences. Names of strains obtained from this study are shown in bold. B, Relative abundances of major culturable denitrifying bacterial communities in the absence or presence of...

During the last decades, one hard coal mine in Germany after the other stopped production and entered the post-mining phase. The last pits that terminated active mining were Ibbenbüren and Prosper-Haniel in 2018. For reasons of cost efficiency, the pumping of mine water is reorganized in this phase and levels are allowed to rise up to a certain depth below the surface. This process goes along with pressure changes in the rock that may e.g....

Spectroscopic methods with their capability to gain information on atoms and molecules are becoming more and more relevant in earth sciences in order to open up new fields of research and to solve problems in the various scientific disciplines. Therefore, it is important to have an exchange, which methods are especially suitable for different requirements. The session Spectroscopic methods in modern geosciences is addressed to all geoscientific researchers who use spectroscopic technologies such as Raman,...

The production of geothermal energy requires the circulation of large volumes of thermal brines reaching up to several 100 L/s. These brines are the product of long-time water-rock interactions at a high temperature and depth leading to various water compositions. The contained ions have been used for the chemical characterization of a reservoir and are further a challenge for the power plant operator due to the possibility of uncontrolled mineral formation. The chemical analysis of...

Future discovery of mineral resources requires a better understanding of the mineralized system at large scale. Metal fluxes in the oceanic crust have direct and indirect impact on the formation and composition of hydrothermal mineralizations in active black smoker systems, but also in their ancient analogues including volcanogenic massive sulfides and possibly in some epithermal-porphyry systems. These fluxes occur at different stages during the evolution of the oceanic crust and in very diverse tectonic environments,...

Abstract Background The failure to scale-up and implement physical activity (PA) interventions in real world contexts, which were previously successful under controlled conditions, may be attributed to the different criteria of stakeholders and scientists in the selection process of available interventions. Therefore, the aim of our study was to investigate and compare the criteria applied by local stakeholders and scientists for selecting amongst suitable school-based PA interventions for implementation. Methods We conducted a three-round repeated...

Karst aquifers constitute important freshwater resources, but are challenging to manage and to protect, because of their unique hydraulic structure and behaviour, representing continuous challenges for research and development. Karst aquifers are widespread and contribute to freshwater supply of most Mediterranean countries and many cities are supplied by karst water, e.g., Rome, Vienna, Montpellier and Beirut. These land surfaces correspond to the main recharge zones of karst aquifers, which are often hydraulically connected over large...

Supplemental material, sj-docx-1-tej-10.1177_20417314231169375 for Single-cell RNA-sequence analysis of human bone marrow reveals new targets for isolation of skeletal stem cells using spherical nucleic acids by Elloise Z Matthews, Stuart Lanham, Kate White, Maria-Eleni Kyriazi, Konstantina Alexaki, Afaf H El-Sagheer, Tom Brown, Antonios G Kanaras, Jonathan J West, Ben D MacArthur, Patrick S Stumpf and Richard OC Oreffo in Journal of Tissue Engineering

Supplementary Material 1

We report on a course project in which students submit weekly probabilistic forecasts of two weather variables and one financial variable. This real-time format allows students to engage in practical forecasting, which requires a diverse set of skills in data science and applied statistics. We describe the context and aims of the course, and discuss design parameters like the selection of target variables, the forecast submission process, the evaluation of forecast performance, and the feedback...

Während der Ausstieg aus der Nutzung der Kernenergie in Deutschland für April 2023 vorgesehen ist, bleibt die Entsorgung des hochradioaktiven Materials eine langfristige Aufgabe, die auch zukünftige Generationen betreffen wird. Mit dem Standortauswahlgesetz (StandAG) gibt der Gesetzgeber die Rahmenbedingungen für das derzeit laufende Verfahren zur Standortauswahl für ein tiefengeologisches Lager in Deutschland vor. Ziel des Verfahrens ist es, für einen Zeitraum von einer Million Jahre bestmögliche Sicherheit zu gewährleisten. Der Zeithorizont bis zu einem Verschluss...

Abstract Background Arbuscular mycorrhizal fungi (AMF) are key soil organisms and their extensive hyphae create a unique hyphosphere associated with microbes actively involved in N cycling. However, the underlying mechanisms how AMF and hyphae-associated microbes may cooperate to influence N2O emissions from “hot spot” residue patches remain unclear. Here we explored the key microbes in the hyphosphere involved in N2O production and consumption using amplicon and shotgun metagenomic sequencing. Chemotaxis, growth and N2O emissions of...

Additional file 3: Fig. S3. Structure of microbial communities harbouring nirK, nirS and clade I nosZ in pot expt 2. A, B, The relative abundance of major taxonomic groups of nirK, nirS and clade I nosZ communities in the absence or presence of AMF at both harvests based on gene (A) and transcript (B) levels (n = 5). T1 and T2, the first (day 24) and second (day 34) harvests, respectively; asterisks, significant differences between...

Additional file 3: Fig. S3. Structure of microbial communities harbouring nirK, nirS and clade I nosZ in pot expt 2. A, B, The relative abundance of major taxonomic groups of nirK, nirS and clade I nosZ communities in the absence or presence of AMF at both harvests based on gene (A) and transcript (B) levels (n = 5). T1 and T2, the first (day 24) and second (day 34) harvests, respectively; asterisks, significant differences between...

There is a wealth of data indicating human bone marrow contains skeletal stem cells (SSC) with the capacity for osteogenic, chondrogenic and adipogenic differentiation. However, current methods to isolate SSCs are restricted by the lack of a defined marker, limiting understanding of SSC fate, immunophenotype, function and clinical application. The current study applied single-cell RNA-sequencing to profile human adult bone marrow populations from 11 donors and identified novel targets for SSC enrichment. Spherical nucleic acids...

Autonome Autos, die den Straßen- und Personenverkehr im Blick haben müssen, oder  Stromsysteme, in denen sich Tausende von Solaranlagen, Windrädern und Elektroautos miteinander abstimmen – die technische Welt, die uns umgibt, wird immer komplexer und vernetzter. Möglich wird das durch immer leistungsfähigere mechanische Komponenten, Mikroprozessoren und intelligente Software, die über das Internet miteinander und mit Steuerzentralen verbunden sind. Für die Entwicklung derart komplexer Systeme werden künftig immer mehr Expertinnen und Experten aus verschiedenen Disziplinen zusammenarbeiten...

Climate, energy transition, raw material supply, underground storage are socially relevant topics that require geoscientific expertise but are not associated with the geosciences. How do you bring topics from the geosciences into society? Geohazards such as earthquakes, landslides, tsunamis, floods and volcanic eruptions are high-profile issues that geoscience experts contribute to in the media. Geoscientists in geoparks also develop active communication strategies to present geoscientific phenomena to society. The session will highlight ways in which...

With its successful application in diverse disciplines, Artificial Intelligence (AI), especially Machine Learning, has recently also become more and more popular in geosciences, particularly in the advent of increasingly affordable high performance computational power and the open source mentality of the AI community. The applications range from spatial classification and regression tasks, time-series forecasting, image/signal processing, anomaly detection, event classification, bias correction, and many others, and include for example artificial neural networks, support vector machines,...

Additional file 7: Fig. S7. Structure of nirK and nirS communities in the absence or presence of AMF. Pot expt 1. Relative abundance of major taxonomic groups of nirK and nirS communities under the −AMF and +AMF treatments in different patches (n = 8). Control, soil patch; NSfaba and Sfaba, patches with unsterilized (NS) or sterilized (S) faba bean residues, respectively; asterisks, significant differences between the −AMF and +AMF treatments in each patch type according...

Am 24. November 2022 veranstaltete das Helmholtz Forum Forschungssoftware eine Informationsveranstaltung zu aktuellen Entwicklungen im Bereich Forschungssoftware in Helmholtz. Das Helmholtz Forum Forschungssoftware wird gemeinsam von der Task Group Forschungssoftware des AK Open Science und dem HIFIS Software Cluster getragen. Die Veranstaltung wurde unter dem Titel „3. Helmholtz Open Science Forum Forschungssoftware“ vom Helmholtz Open Science Office organisiert. Eine erste Veranstaltung des Helmholtz Forum Forschungssoftware fand im Mai 2021 und eine zweite im April 2022...

Graphical program for display time dependent data on 3D finite elment meshes

While the use of nuclear energy is set to be phased out in Germany by mid-April 2023, the management of high-level radioactive material remains a long-term task that will also concern future generations. With the Site Selection Act (StandAG), legislators have defined the regulatory framework for the site selection procedure currently under way for a deep geological repository in Germany. The aim of the procedure is to ensure the greatest possible safety for a period...

This session thematizes the formation, evolution and economic importance of carbonatites and alkaline rocks. These play an important role for our society, as they contain exceptional high levels of critical raw materials, such as REE, Nb, P and F, to name a few. However, to understand how such deposits form, we need to understand how their rocks and mineral assemblages formed in space and time. Although our knowledge about the formation and mineralization as well...