378 Works

Data for: Tunable self-cleaving ribozymes for modulating gene expression in eukaryotic systems

Thomas Jacobsen, Gloria Yi, Hadel Al Asafen, Ashley Jermusyk, Chase Beisel & Gregory Reeves
Advancements in the field of synthetic biology have been possible due to the development of genetic tools that are able to regulate gene expression. However, the current toolbox of gene regulatory tools for eukaryotic systems have been outpaced by those developed for simple, single-celled systems. Here, we engineered a set of gene regulatory tools by combining self-cleaving ribozymes with various upstream competing sequences that were designed to disrupt ribozyme self-cleavage. As a proof-of-concept, we were...

Genomic population structure of striped bass (Morone saxatilis) from the Gulf of St. Lawrence to Cape Fear River

Nathalie LeBlanc, Benjamin Gahagan, Samuel Andrews, Trevor Avery, Gregory Puncher, Benjamin Reading, Colin Buhariwalla, R Allen Curry, Andrew Whitely & Scott Pavey
Striped Bass, Morone saxatilis (Walbaum, 1792), is an anadromous fish species that supports fisheries throughout North America and is native to the North American Atlantic Coast. Due to long coastal migrations that span multiple jurisdictions, a detailed understanding of population genomics is required to untangle demographic patterns, understand local adaptation, and characterize population movements. This study used 1256 single nucleotide polymorphism (SNP) loci to investigate genetic structure of 477 Striped Bass sampled from 15 locations...

Data from: Bee phenology is predicted by climatic variation and functional traits

Michael Stemkovski, Will Pearse, Sean Griffin, Gabriella Pardee, Jason Gibbs, Terry Griswold, John Neff, Ryan Oram, Molly RightMyer, Cory Sheffield, Karen Wright, Brian Inouye, David Inouye & Rebecca Irwin
Climate change is shifting the environmental cues that determine the phenology of interacting species. Plant-pollinator systems may be susceptible to temporal mismatch if bees and flowering plants differ in their phenological responses to warming temperatures. While the cues that trigger flowering are well-understood, little is known about what determines bee phenology. Using Generalized Additive Models, we analyzed time-series data representing 67 bee species collected over nine years in the Colorado Rocky Mountains to perform the...

Relative reproductive phenology and synchrony affect neonate survival in a nonprecocial ungulate

Eric Michel, Bronson Strickland, Stephen Demarais, Jerrold Belant, Todd Kautz, Jared Duquette, Dean Beyer, Michael Chamberlain, Karl Miller, Rebecca Shuman, John Kilgo, Duane Diefenbach, Bret Wallingford, Justin Vreeland, Steve Ditchkoff, Christopher DePerno, Christopher Moorman, Michael Chitwood & Marcus Lashley
1. Degree of reproductive synchronization in prey is hypothesized as a predator defense strategy reducing prey risk via predator satiation or predator avoidance. Species with precocial young, especially those exposed to specialist predators, should be highly synchronous to satiate predators (predator satiation hypothesis), while prey with nonprecocial (i.e., altricial) young, especially those exposed to generalist predators, should become relatively asynchronous to avoid predator detection (predator avoidance hypothesis). The white-tailed deer (Odocoileus virginianus) in North America...

Compensatory growth and costs of molluscivory in Gambusia holbrooki

Brian Langerhans, Taylor Goins, Kenzi Stemp & Rüdiger Riesch
Some prey are exceptionally difficult to digest, and yet even non-specialized animals may consume them—why? Durophagy, the consumption of hard-shelled prey, is thought to require special adaptations for crushing or digesting the hard shells to avoid the many potential costs of this prey type. But many animals lacking specializations nevertheless include hard-bodied prey in their diets. We describe several non-mutually exclusive adaptive mechanisms that could explain such a pattern, and point to optimal foraging and...

Outdoor activity participation improves adolescents’ mental health and well-being during the COVID-19 pandemic

Steven Jackson, Kathryn Stevenson, Lincoln Larson, Nils Peterson & Erin Seekamp
The COVID-19 pandemic is reshaping human interactions with the natural environment, generating profound consequences for health and well-being. To assess the effects of COVID-19 on the outdoor recreation participation and well-being of adolescents we conducted a nationally representative survey of youth ages 10-18 across the United States (n = 624) using a Qualtrics XM panel between April 30 and June 15, 2020. Survey questions focused on frequency of participation in several types of outdoor activities...

Legacy effect of grazing intensity mediates the bottom-up controls of resource addition on soil food webs

Dima Chen, Bing Wang, Ying Wu, Shuijin Hu & Yongfei Bai
1. Large-scale studies have demonstrated that nitrogen (N) and water (W) availability greatly affect terrestrial ecosystems worldwide, and this is especially true for the resource-poor semi-arid grasslands. Yet, experimental evidence is lacking for how N and W availability affect soil food webs across historical grazing intensity-altered environments at a local scale. 2. Here, we included N- and W-addition treatments in an 8-year grazing experiment (with four grazing intensities) to determine how the legacy effects of...

Drought legacy affects microbial community trait distributions related to moisture along a savannah grassland precipitation gradient

Ainara Leizeaga, Lettice C. Hicks, Lokeshwaran Manoharan, Christine V. Hawkes & Johannes Rousk
Ecosystem models commonly use stable-state assumptions to predict responses of soil microbial functions to environmental change. However, past climatic conditions can shape microbial functional responses resulting in a “legacy effect”. For instance, exposure to drier conditions in the field may shape how soil microbial communities respond to subsequent drought and drying and rewetting events. We investigated microbial tolerance to low moisture levels (“resistance”) and ability to recover after a drying and rewetting (DRW) perturbation (“resilience”)...

Deconstructing incubation behaviour in response to ambient temperature over different timescales

David Diez-Méndez, Caren Cooper, Juan José Sanz, Jose Verdejo & Emilio Barba
Avian embryos need a stable thermal environment to develop optimally, while incubating females need to allocate time to self-maintenance off the nest. In species with female-only incubation, eggs are exposed to ambient temperatures that usually cool them down during female absences. The lower the ambient temperature the sooner females should return to re-warm the eggs. When incubation constraints ease at increasing ambient temperatures, females respond by increasing either incubation effort or self-maintenance time. These responses...

National survey of college students' beliefs, attitudes, and behaviors related to hunting, fishing, and wildlife conservation

Lincoln Larson
This dataset contains responses from an online survey of diverse undergraduate students (ages 18-34 years) attending 22 different universities across 22 U.S. states (total n = 17,203). Survey questions focused on students' beliefs, attitudes, and behaviors related to hunting, fishing, and wildlife conservation. The survey was conducted from 2018-2020.

Data for: Many-body thermodynamics on quantum computers via partition function zeros

Akhil Francis, Daiwei Zhu, Cinthia Huerta Alderete, Sonika Johri, Xiao Xiao, James K. Freericks, Christopher Monroe, Norbert M. Linke & Alexander F. Kemper
Partition functions are ubiquitous in physics: they are important in determining the thermodynamic properties of many-body systems, and in understanding their phase transitions. As shown by Lee and Yang, analytically continuing the partition function to the complex plane allows us to obtain its zeros and thus the entire function. Moreover, the scaling and nature of these zeros can elucidate phase transitions. Here we show how to find partition function zeros on noisy intermediate-scale trapped ion...

Schizophrenia and Bartonella spp. infection: A pilot case–control study

Erin Lashnits, Ricardo Maggi, Fredrik Jarskog, Julie Bradley, Edward Breitschwerdt & Flavio Frohlich
Recently, infections with emerging zoonotic bacteria of the genus Bartonella have been reported in association with a range of CNS symptoms. Currently, it remains unknown if Bartonella spp. infection is associated with symptoms of schizophrenia/schizoaffective disorder (SCZ/SAD). The objective of this study was to determine if there is an association between Bartonella species infection and SCZ/SAD. A secondary objective was to determine if SCZ/SAD symptoms were more severe among participants with documented Bartonella spp. infection....

Data from: Mammal-bearing gastric pellets potentially attributable to Troodon formosus at the Cretaceous Egg Mountain locality, Two Medicine Formation, Montana, U.S.A.

William Freimuth, David Varricchio, Alexandria Brannick, Lucas Weaver & Gregory Wilson Mantilla
Fossil gastric pellets (regurgitalites) have distinct taphonomic characteristics that facilitate inferences of behavioural ecology in deep time, despite their rarity in the fossil record. Using the taphonomic patterns of both extant and fossil small mammals from more recent geologic deposits as a guide, we assess the taphonomy of three unusual multi-individual aggregates of mammal skeletons from paleosols at Egg Mountain, a dinosaur nesting locality from the Upper Cretaceous Two Medicine Formation, Montana, USA. One aggregate...

A greenhouse experiment partially supports inferences of ecogeographic isolation from niche models of Clarkia sister species

Kathleen Kay, Kaleb Goff & Cormac Martinez Del Rio
Premise: Ecogeographic isolation, or geographic isolation caused by ecological divergence, is thought to be of primary importance in speciation, yet is difficult to demonstrate and quantify. To determine whether distributions are limited by divergent adaptation or historical contingency, the gold standard is to reciprocally transplant taxa between their geographic ranges. Alternatively, ecogeographic isolation is inferred from species distribution models and niche divergence tests based on widely available environmental and occurrence data. Methods: We test for...

Resonance Raman confirms partial hemoglobin preservation in dinosaur remains

Brandon Long
Still-soft, hollow, and flexible structures that are morphologically consistent with blood vessels were recovered from demineralized dinosaur bone and studied with resonance Raman techniques to test the hypothesis that these vessel-like structures are original to the dinosaur, and that they maintain endogenous molecular characteristics. We probed these ancient samples using resonance Raman at two different wavelengths, and the existence of a stronger resonance Raman signal level in the green compared to blue excitation is consistent...

R/QTL datasets for fusiform rust resistance QTL mapping

Fikret Isik & Edwin Lauer
Fusiform rust disease, caused by the endemic fungus Cronartium quercuum f. sp. fusiforme, is the most damaging disease affecting economically important pine species in the southeast United States. In this report, we detail the genomic localization and sequence-level discovery of candidate race-nonspecific broad-spectrum fusiform rust resistance genes in Pinus taeda L. Two full-sib families, each with ~1000 progeny, were challenged with a complex inoculum consisting of over 150 pathogen isolates. High-density linkage mapping revealed three...

Searching for deep-seated thrust faults on the moon

Matthew Collins
The lunar maria are large expanses of basalt that infill antecedent impact basins and show evidence for post-emplacement deformation. Landforms within many of these basins suggest a period of compressive tectonics, although the mechanism for their formation remains an open question. Previous work for Mare Crisium demonstrated that basin-circumferential wrinkle ridges, which typically demarcate the inner edge of an annulus of elevated terrain, are the result of deep-seated thrust faults that preferentially form along the...

Data for: Predation risk and the evolution of a vertebrate stress response: parallel evolution of stress reactivity and sexual dimorphism

Jerker Vinterstare, Gustaf MO Ekelund Ugge, Kaj Hulthén, Alexander Hegg, Christer Brönmark, P Anders Nilsson, U Ronja Zellmer, Marcus Lee, Varpu Pärssinen, Yongcui Sha, Caroline Björnerås, Huan Zhang, Raphael Gollnisch, Simon David Herzog, Lars-Anders Hansson, Martin Škerlep, Nan Hu, Emma Johansson & R Brian Langerhans
Predation risk is often invoked to explain variation in stress responses. Yet, the answers to several key questions remain elusive, including: 1) how predation risk influences the evolution of stress phenotypes, 2) the relative importance of environmental versus genetic factors in stress reactivity, and 3) sexual dimorphism in stress physiology. To address these questions, we explored variation in stress reactivity (ventilation frequency) in a post-Pleistocene radiation of live-bearing fish, where Bahamas mosquitofish (Gambusia hubbsi) inhabit...

Microevolutionary change in mimicry? Erosion of rattling behaviour among nonvenomous snakes on islands lacking rattlesnakes

Bradley Allf, Amanda Sparkman & David Pfennig
Batesian mimics––harmless species that converge on the warning signals of a dangerous species––are spectacular examples of adaptation, but few documented cases involve acoustic signals. Even fewer studies have documented microevolutionary change in mimicry of any kind. Here, we describe potential evolutionary change in acoustic mimicry. Many nonvenomous snakes vibrate their tail tip when threatened, making a sound resembling a venomous rattlesnake. When we compared this behaviour between gopher snakes from mainland California where rattlesnakes are...

Environmental decomposition of olefinic cuticular hydrocarbons of Periplaneta americana generates a volatile pheromone that guides social behaviour

Eduardo Hatano, Ayako Wada-Katsumata & Coby Schal
Once emitted, semiochemicals are exposed to reactive environmental factors that may alter them, thus disrupting chemical communication. Some species, however, might have adapted to detect environmentally mediated breakdown products of their natural chemicals as semiochemicals. We demonstrate that air, water vapour and ultraviolet (UV) radiation break down unsaturated cuticular hydrocarbons (CHCs) of Periplaneta americana (American cockroach), resulting in the emission of volatile organic compounds (VOCs). In behavioural assays, nymphs strongly avoided aggregating in shelters exposed...

The distribution and spread of naturally occurring Medea selfish genetic elements in the United States

Sarah Cash, Fred Gould & Marce Lorenzen
Selfish genetic elements (SGEs) are DNA sequences that are transmitted to viable offspring in greater than Mendelian frequencies. Medea SGEs occur naturally in some populations of red flour beetle (Tribolium castaneum) and are expected to increase in frequency within populations and spread among populations. The large‐scale U.S. distributions of Medea‐4 (M4) had been mapped based on samples from 1993 to 1995. We sampled beetles in 2011–2014 and show that the distribution of M4 in the...

Anonymized Researcher Interview Data - from the Raising the Profile of the NCSU Libraries Research Support Strategies & Engagement project

Hilary Davis & Colin Nickels
The data are from semi-structured qualitative interviews conducted with researchers (all status levels from undergraduate to tenured faculty) at NC State University between August 2018 - January 2019

Code of Best Practices in Fair Use for Open Educational Resources

Meredith Jacob, Peter Jaszi, Prudence S. Adler & William Cross

Modeling Low Carbon Energy Futures for the United States

Joseph DeCarolis

Annotated transcriptome data from: Transcriptome annotation reveals minimal immunogenetic diversity among Wyoming toads, Anaxyrus baxteri

Jeffrey Yoder, Kara Carlson, Dustin Wcisel, Hayley Ackerman, Jessica Romanet, Emily Christiansen, Jennifer Niemuth, Christina Williams, Matthew Breen, Michael Stoskopf & Alex Dornburg
Briefly considered extinct in the wild, the future of the wild population of the Wyoming toad (Anaxyrus baxteri) continues to rely on captive breeding to supplement the wild population. Given its small natural geographic range and history of rapid population decline at least partly due to fungal disease, investigation of the diversity of key receptor families involved in the host immune response represents an important conservation need. Population decline may have reduced immunogenetic diversity sufficiently...

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  • North Carolina State University
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  • Michigan State University
  • University of Massachusetts Amherst