Triggering receptor expressed on myeloid cells 1 (TREM1) participates in the development of endometritis. This study aims at identifying the effects and interaction of TREM1 and upstream stimulatory factor 2 (USF2) in endometritis by using a model of lipopolysaccharide (LPS)-induced human endometrial epithelial cells (HEnEpCs). ELISA was performed to determine the levels of interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF-α) after LPS stimulation. TREM1 and USF2 expression was examined with RT-qPCR and Western blot....

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) can target cardiomyocytes (CMs) to directly invade the heart resulting in high mortality. This study aims to explore the biological characteristics of SARS-CoV-2 infected myocardium based on omics by collecting transcriptome data and analyzing them with a series of bioinformatics tools. Totally, 86 differentially expressed genes (DEGs) were discovered in SARS-CoV-2 infected CMs, and 15 miRNAs were discovered to target 60 genes. Functional enrichment analysis indicated that these...

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) can target cardiomyocytes (CMs) to directly invade the heart resulting in high mortality. This study aims to explore the biological characteristics of SARS-CoV-2 infected myocardium based on omics by collecting transcriptome data and analyzing them with a series of bioinformatics tools. Totally, 86 differentially expressed genes (DEGs) were discovered in SARS-CoV-2 infected CMs, and 15 miRNAs were discovered to target 60 genes. Functional enrichment analysis indicated that these...

Abstract Background COVID-19 is a disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Rigorous detection and treatment strategies against SARS-CoV-2 have become very challenging due to continuous evolutions to the viral genome. Therefore, careful genomic analysis is sorely needed to understand transmission, the cellular mechanism of pathogenicity, and the development of vaccines or drugs. Objective In this study, we intended to identify SARS-CoV-2 genome variants that may help understand the cellular and molecular...

Additional file 4.

Abstract Background Autism spectrum disorder (ASD) is often accompanied by intellectual disability (ID). Despite extensive studies, however, the genetic basis for this comorbidity is still not clear. In this study, we tried to develop an analyzing pipeline for de novo mutations and possible pathways related to ID phenotype in ASD. Whole-exome sequencing (WES) was performed to screen de novo mutations and candidate genes in 79 ASD children together with their parents (trios). The de novo...

Abstract Background Prophylactic antibiotic use during delivery is common in routine obstetric practice to prevent infection globally, especially in low- and middle-income countries. In China, however, little is currently known about the national estimates for prophylactic antibiotic use during delivery. Therefore, we aimed to describe the prevalence of prophylactic antibiotic use and guideline adherence using national data in China. Methods This cross-sectional study analyzed a national dataset from the China Labor and Delivery Survey in...

Abstract Background Prophylactic antibiotic use during delivery is common in routine obstetric practice to prevent infection globally, especially in low- and middle-income countries. In China, however, little is currently known about the national estimates for prophylactic antibiotic use during delivery. Therefore, we aimed to describe the prevalence of prophylactic antibiotic use and guideline adherence using national data in China. Methods This cross-sectional study analyzed a national dataset from the China Labor and Delivery Survey in...

It is known that phase conjugation technique is achieved by the nonlinearity of materials, and widely adopted in various applications, such as high-resolution imaging, signal amplification, and target detecting. Here, we have proposed a field-enhancement method for the degenerate four wave mixing (FWM) for phase conjugation purpose. In this method, a thin metasurface with nonlinear property is utilized to confine and guide the pumps, achieving the enhanced FWM within a flexible structure. Compared to existing...

We propose and experimentally demonstrate the generation of a circular polarization detector based on planar polarization holography. The detector is designed by constructing the interference field according to the null reconstruction effect (NRE). We create the multiplexed holograms which feature the combination of two sets of hologram patterns and operate with opposite circular polarization beams. In a few seconds, the exposure operation allows the polarization multiplexed holograms element to be generated, with functionality equivalent to...

Additional file 1: Fig. S1. Enrolment and follow-up of participants. Table S1. Comparison of the baseline characteristics of children followed up and lost to follow-up and those of their mothers. Table S2. Comparison of baseline characteristics of children with different anti-HBs levels at the age of 7 months and those of their mothers. Table S3. Genotype, subtype, homology and S region mutation comparisons of infected mother-child HBV pairs. Table S4. Comparison of the baseline characteristics...

Advanced oxidation processes (AOPs) based on ozone are gaining continuously growing popularity in wastewater treatment. This study explored the treatment of coking wastewater using a combination of ozonation (O3), ultraviolet (UV), and hydrogen peroxide (H2O2) process expressed by % chemical oxygen demand (COD) removal, % total organic carbon (TOC), % UV254, % fluorescence intensity removal and its electrical energy consumption. The obtained results demonstrated that, the combination of O3, UV, and H2O2 which is denoted...

Additional file1: Fig. S1. OUM1 knockdown impairs UM cell metastasis and proliferation. Fig. S2. GO and pathway function analysis. Fig. S3. Bioinformatics analysis of OUM1 targets. Fig. S4. PTPRZ1 might be a target of OUM1. Fig. S5. Synergistic effect of SOV and OUM1 interference on apoptosis. Fig. S6. In vivo toxicity evaluation. Table S1. Downregulated and Upregulated gene. Table S2. Primers and siRNA used in this study. Table S3. Primers used in RNA-ChIP assay. Details...

Additional file 1.

Additional file 1.

Abstract Acute promyelocytic leukemia (APL) is highly aggressive and is frequently associated with disseminated intravascular coagulation and high early death rates. Although all-trans retinoic acid (RA) induces complete remission in a high proportion of patients with APL, there are limited treatments for APL patients with RA resistance. Here we report an atypical APL patient, with an APL-like disease that developed very slowly without anti-leukemia therapy for nearly 2 years. During that time, the patient only...

Abstract Background Accompanied by the decline of physiological functions, the decrease of physical activity, and comorbidities, older adults are susceptible to sarcopenia because of accelerated loss of muscle mass. Resistance training is recommended by different clinical practice guidelines. However, most older adults have difficulty in taking recommended high-load resistance training programs, and there are limited exercise options form them. Baduanjin, a traditional Chinese mind-body exercise with relatively low intensity and simple movements, has the potential...

Abstract Objective Community-acquired pneumonia (CAP) is associated with elevated morbidity and mortality, and it usually occurs in older adults. Our goal here was to assess the efficacies of muscle mass-related biomarkers, such as, aspartate transaminase/alanine transaminase (AST/ALT) and creatinine/cystatin C*100 (Cr/CysC*100), in predicting 1-, 2-, and 3-year mortalities of older CAP patients. Methods Design: Retrospective cohort study. Setting and Participants: A teaching hospital in western China. Hospitalized CAP patients, aged≥60 years. We separated patients into...

Main Dataset;Subsidiary Data matrix 2;Subsidiary Data matrix 3;Subsidiary Data matrix 4

Additional file 2: Figure S1. Depletion of LARRPM promoted proliferation, repressed apoptosis and promoted migration and invasion of LUAD cells. A LARRPM expression in HCC827 cells with LARRPM stable depletion or control was detected by qRT-PCR. B Cell proliferation of HCC827 cells with LARRPM stable depletion or control was detected using CCK-8 assays. C Cell proliferation of HCC827 cells with LARRPM stable overexpression or control was detected using EdU incorporation assays. Scale bar: 100 µm....

Additional file 6: Fig. S5. Anti-CSF1 antibody abolished the effects of LARRPM on M2 macrophage infiltration. A THP-1 cells were subjected to transwell migration assays towards A549 cells with LARRPM overexpression or A549 control cells treated with anti-CSF1. Scale bar: 100 µm. B THP-1 cells were subjected to transwell migration assays towards HCC827 cells with LARRPM depletion or HCC827 control treated with anti-CSF1. Scale bar: 100 µm. C M1 and M2 polarization markers expression in...

Abstract Background Long non-coding RNAs (lncRNAs) are critical regulators in lung adenocarcinoma (LUAD). M2-type tumor-associated macrophages (TAMs) also play oncogenic roles in LUAD. However, the involvement of lncRNAs in TAM activation is still largely unknown. Methods The expressions of LARRPM, LINC00240 and CSF1 were determined by RT-qPCR. The regulation of LINC00240 and CSF1 by LARRPM was investigated by RNA–protein pull-down, RNA immunoprecipitation, chromatin immunoprecipitation and bisulfite DNA sequencing. In vitro and in vivo gain- and...

Abstract Background Long non-coding RNAs (lncRNAs) are critical regulators in lung adenocarcinoma (LUAD). M2-type tumor-associated macrophages (TAMs) also play oncogenic roles in LUAD. However, the involvement of lncRNAs in TAM activation is still largely unknown. Methods The expressions of LARRPM, LINC00240 and CSF1 were determined by RT-qPCR. The regulation of LINC00240 and CSF1 by LARRPM was investigated by RNA–protein pull-down, RNA immunoprecipitation, chromatin immunoprecipitation and bisulfite DNA sequencing. In vitro and in vivo gain- and...

Additional file 1. Supplementary methods.

Additional file 1. Supplementary methods.