Additional file 7: Figure S7. Validation of the CREB inhibitor 666-15 in TREM2−/− mice following LPS treatment. A–C Western blot analysis (A) and quantification of p-CREB and CREB (B, C) in TREM2−/− mice after treatments with LPS and 666-15, a potent and selective CREB inhibitor. Data are presented as mean ± SEM and analyzed by unpaired two-tailed Student’s t test (compared to LPS, ****p < 0.001). n = 4 mice in each group.

Additional file 8: Figure S8. Validation of TPM1 and TREM2 in BV2 cells after transfection with control plasmid, TPM1 plasmid, or TPM1 and Trem2 plasmids. A mRNA level of TPM1 in BV2 cells after transfection with control plasmid, TPM1 plasmid, or TPM1 and Trem2 plasmids. Data are presented as mean ± SEM and analyzed by one-way ANOVA with Tukey’s multiple comparison test (compared to TPM1 plasmid, **p < 0.01). Five independent experiments were performed. B–D...

Additional file 9: Figure S9. DNA sequencing for detecting Rd8-associated single nucleotide deletion. F: Forward; R: Reverse.

Additional file 2: Table S2. Exonic mutation profile of 53 pGI-DLBCL patients.

Additional file 4: Table S4. Summary of the statistically significant correlations in the matrix.

Additional file 5: Table S5. Summary of IGLL5 and LRP1B mutations in pGI-DLBCL.

Abstract Background Diffuse large B-cell lymphoma (DLBCL) is the most common aggressive non-Hodgkin lymphoma, and about 10% of DLBCL cases primarily occur in the gastrointestinal tract. Previous reports have revealed that primary gastrointestinal-DLBCL (pGI-DLBCL) harbors different genetic mutations from other nodal or extranodal DLBCL. However, the exonic mutation profile of pGI-DLBCL has not been fully addressed. Methods We performed whole-exome sequencing of matched tumor tissues and blood samples from 53 pGI-DLBCL patients. The exonic mutation...

Abstract Background Flavonoid C-glycosides have many beneficial effects and are widely used in food and medicine. However, plants contain a limited number of flavonoid C-glycosides, and it is challenging to create these substances chemically. Results To screen more robust C-glycosyltransferases (CGTs) for the biosynthesis of flavonoid C-glycosides, one CGT enzyme from Stenoloma chusanum (ScCGT1) was characterized. Biochemical analyses revealed that ScCGT1 showed the C-glycosylation activity for phloretin, 2-hydroxynaringenin, and 2-hydroxyeriodictyol. Structure modeling and mutagenesis experiments...

Additional file 3. Behavioral bouts analysis. For each behavior scored, the duration of each bout was summarized by mean or median across the entire parental behavior test for each animal. Each data point represents a distinct animal, and the boxplots depict the median (thick horizontal line) and the 25th and 75th percentiles.

Advanced oxidation processes (AOPs) based on ozone are gaining continuously growing popularity in wastewater treatment. This study explored the treatment of coking wastewater using a combination of ozonation (O3), ultraviolet (UV), and hydrogen peroxide (H2O2) process expressed by % chemical oxygen demand (COD) removal, % total organic carbon (TOC), % UV254, % fluorescence intensity removal and its electrical energy consumption. The obtained results demonstrated that, the combination of O3, UV, and H2O2 which is denoted...

Background:Although the Maastricht VI/Florence consensus report recommended high-dose proton pump inhibitor–amoxicillin dual therapy as possible rescue therapy for Helicobacter pylori infection, clinical evidence of its efficacy was lacking.Objectives:To compare the efficacy, safety, patient compliance, and cost between high-dose dual therapy (HDDT) and culture-based susceptibility-guided therapy (CB-SGT) as a rescue regimen for H. pylori infection.Design:A single-center, open-label, randomized controlled clinical trial.Methods:In all, 146 patients with a history of eradication failure were enrolled and randomly assigned to...

Supplemental material, sj-doc-1-tag-10.1177_17562848221142925 for Efficacy and safety of high-dose esomeprazole and amoxicillin dual therapy versus bismuth-containing quadruple therapy for Helicobacter pylori infection: a multicenter, randomized controlled clinical trial by Hao Mei, Yan Guo, Jing-Tao Zhao, Jun Yang, Wen-jing Sun, De-kui Zhang, Ping He, Gang Shi, Na-yun Su, Ran Han and Chun-Hui Lan in Therapeutic Advances in Gastroenterology

Background:A high-dose proton pump inhibitor (PPI)-amoxicillin dual therapy has been investigated for treatment of patients with Helicobacter pylori (H. pylori) infection. Currently, the efficacy of this dual therapy remains inconclusive, with controversial findings from various single-center clinical trials.Objectives:To assess the efficacy and safety of high-dose dual therapy (HDDT) compared with the bismuth-containing quadruple therapy (BQT) in treatment-naive patients with H. pylori infection.Design:A multicenter, open-label, randomized controlled clinical trial.Methods:Three hundred and forty treatment-naïve patients with H....

Additional file 3: Fig. S1. Analysis of simple sequence repeats (SSRs) in the cp genomes of three Amomum species. Fig. S2. Frequency of identified SSR motifs in different repeat class types. Fig. S3. Repeat sequences of three Amomum species. F, P, R, and C indicate the repeat types F (forward), P (palindrome), R (reverse), and C (complement), respectively. Fig. S4. ML tree based on ccsA sequences of Amomum species. This Bootstrap consensus tree was constructed...

Abstract Background Species in genus Amomum always have important medicinal and economic values. Classification of Amomum using morphological characters has long been a challenge because they exhibit high similarity. The main goals of this study were to mine genetic markers from cp genomes for Amomum species identification and discover their evolutionary history through comparative analysis. Results Three species Amomum villosum, Amomum maximum and Amomum longipetiolatum were sequenced and annotated for the complete chloroplast (cp) genomes,...

Middle cerebral artery occlusion(MCAO) is chemia-reperfusion in rats is one of the commonly used in vivo models in stroke research,but the problems of long time-consuming, unstable neurological injury and high mortality are not conducive to an experimental animal study.In this study, the success rate of the MCAO model in rats was improved by changing the surgical approach and giving preoperative as well as postoperative care support. Methods: Place A-wire below the common carotid artery(CCA)to ligate...

The database includes major ion (Ca, Mg, Na, K, HCO3, SO4, and Cl) data spanning 1940-2019, with 9487 groups of chemical results from 1434 water samples collected mainly in six large river basins in eastern Siberia.

Supplementary Information

About how to get the criterion to judge the maximum limitation of the dispersed fringe

Child abuse is considered to be an essential factor in the development of aggressive behavior. The intensity of the positive relations between child abuse and aggressive behavior differed considerably among researches despite the fact that abundant studies have observed this relation. According to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) approach, a three-level meta-analysis was employed to obtain reliable estimates for the sizes of effects and investigate some potential moderators of the...

Additional file 3: Fig. S1. Analysis of simple sequence repeats (SSRs) in the cp genomes of three Amomum species. Fig. S2. Frequency of identified SSR motifs in different repeat class types. Fig. S3. Repeat sequences of three Amomum species. F, P, R, and C indicate the repeat types F (forward), P (palindrome), R (reverse), and C (complement), respectively. Fig. S4. ML tree based on ccsA sequences of Amomum species. This Bootstrap consensus tree was constructed...

Additional file 1: Supplementary figure 1. Functional enrichment analyses of differentially expressed genes (DEGs) in chronic lymphocytic leukemia (CLL) cell lines. (A) GO analysis results showed that changes in MF, CC and BP of DEGs between CLL cell lines and control were mainly enriched in sequence−specific DNA binding, Golgi membrane and cation transmembrane transport. (B) KEGG enrichment analysis of DEGs in CLL cell lines and control were mainly enriched in mTOR signaling pathway, NF-kappaB signaling...

Additional file 1: Supplementary figure 1. Functional enrichment analyses of differentially expressed genes (DEGs) in chronic lymphocytic leukemia (CLL) cell lines. (A) GO analysis results showed that changes in MF, CC and BP of DEGs between CLL cell lines and control were mainly enriched in sequence−specific DNA binding, Golgi membrane and cation transmembrane transport. (B) KEGG enrichment analysis of DEGs in CLL cell lines and control were mainly enriched in mTOR signaling pathway, NF-kappaB signaling...

Additional file 3: Supplementary figure 3. The expression of TRIM34, SLC30A10, HOXD4 and circ_0007675 in CLL patients and normal B cells.(A) The expression of TRIM34 in patient specimens were significantly increased. (B) The expression of SLC30A10 in patient specimens were significantly increased. (C) The expression of HOXD4 in patient specimens were significantly increased. (D) The expression of circ_0007675 in patient specimens were significantly increased. All results are expressed as mean ± SEM.

Additional file 4: Supplementary figure 4. Efficiency of miR-185-3p transfection and functional enrichment analyses of target genes of circ_0002078. (A) Efficiency verification of miR-185-3p overexpression in MEC-1 cells by qRT-PCR. (B) GO analysis results demonstrated that the target genes of circ_0002078 were enriched in positive regulation of gene expression, lymphocyte proliferation, and regulation of B cell apoptotic process. (C) KEGG pathway analysis showed that the target genes were primarily relevant to JAK-STAT signaling pathway and...