Additional file 1. Supplemental Figures.

Abstract Background The role of the sputum microbiome in chronic obstructive pulmonary disease (COPD) progression remains elusive. As the advent of the new culture-independent microbial sequencing technique makes it possible to disclose the complex microbiome community of the respiratory tract. The aim of this study was to use metagenomic next-generation sequencing (mNGS) to confirm whether there are differences in sputum microbiome of COPD between different exacerbation frequencies and lung function. Methods Thirty-nine COPD patients were...

Additional file 10: Fig. S2. The location of the next ovulated egg in the oviduct at different time points following oviposition.

Additional file 2: Table S1. Differentially upregulated genes in DBS group at 16 h compared with 4 h following oviposition.

Additional file 3: Table S2. Differentially upregulated genes in DBS group at 22 h compared with 4 h following oviposition.

Additional file 4: Table S3. Differentially upregulated genes in DBS group compared with WS group at 16 h following oviposition.

Additional file 1. RHC and BPA procedure.

Additional file 2: Figure S1. The baseline echocardiographic and hemodynamic characteristics stratified by the modified COMERA 2.0 risk score. Figure S2. The baseline echocardiographic and hemodynamic characteristics stratified by the hybrid COMERA 2.0 risk score.

Additional file 2: Figure S2. SOX21-AS1 silence suppressed the progression of PC. A-G Loss-of-function assays were performed in another two PC cell lines (CFPAC-1 and BxPc3) to further verify the malignant cell behaviors including proliferation, migration, EMT as well as apoptosis upon SOX21 silence treatment. **P < 0.01.

Additional file 3: Figure S3. SOX21-AS1 affected PC cell proliferation, apoptosis, migration, stemness and EMT via modulating SOX21 expression. Rescue experiments in PC cells transfected with shRNA, sh-SOX21-AS1#1 and sh-SOX21-AS1#1 + pcDNA-SOX21, respectively. A-B Cell proliferation detection. C Cell apoptosis detection. D Transwell assays detected the migration ability. E Sphere formation assays detected the stemness. F Western blot analyzed the protein levels of EMT markers and transcription factors. **P < 0.01.

Abstract Background Pancreatic cancer (PC) is a highly malignant tumor which threatens human’s health. Long non-coding RNAs (lncRNAs) are implicated in many cancers, including PC, but their mechanisms in PC have not yet been entirely clarified. We focused on revealing the potential function of lncRNA SOX21-AS1 in PC. Methods Functional assays assessed SOX21-AS1 function on PC progression. Bioinformatics analysis, along with mechanism assays were taken to unmask the regulatory mechanism SOX21-AS1 may exert in PC...

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Abstract Background Dendrobium officinale Kimura et Migo, which contains rich polysaccharides, flavonoids and alkaloids, is a Traditional Chinese Medicine (TCM) with important economic benefits, while various pathogens have brought huge losses to its industrialization. NBS gene family is the largest class of plant disease resistance (R) genes, proteins of which are widely distributed in the upstream and downstream of the plant immune systems and are responsible for receiving infection signals and regulating gene expression respectively....

Additional file 9. Verification reports of astrin-KO cell line.

Abstract Purpose The mixed Candida/bacterial bloodstream infections (mixed C/B-BSIs) is worthy of particular attention recently, and we analyzed the incidence, co-pathogens, clinical characteristics, risk factors, and outcomes of mixed C/B-BSIs compared with monomicrobial candidemia (mono-candidemia) in adult patients in China. Methods All hospitalized adults with candidemia were recruited for this retrospective observational study from January 1, 2013, to December 31, 2019. Results Of the 296 patients with candidemia, 78 cases (26.3%) were mixed C/B-BSIs. Candida...

Abstract Purpose The mixed Candida/bacterial bloodstream infections (mixed C/B-BSIs) is worthy of particular attention recently, and we analyzed the incidence, co-pathogens, clinical characteristics, risk factors, and outcomes of mixed C/B-BSIs compared with monomicrobial candidemia (mono-candidemia) in adult patients in China. Methods All hospitalized adults with candidemia were recruited for this retrospective observational study from January 1, 2013, to December 31, 2019. Results Of the 296 patients with candidemia, 78 cases (26.3%) were mixed C/B-BSIs. Candida...

Abstract Background The present study aimed to evaluate the effects of hepatitis B virus (HBV) or hepatitis C virus (HCV) infection on the risk of cervical cancer. Methods We conducted a case–control study including 838 cervical cancer cases and 838 benign disease controls matched for age, ethnicity, and place of birth. Venous blood was tested for HBV and HCV serological markers. Multiple odds ratios (OR) and corresponding 95% confidence intervals (CI) for cervical cancer were...

Purpose: Previous studies have demonstrated that tone identification can be facilitated when auditory tones are integrated with visual information that depicts the pitch contours of the auditory tones (hereafter, visual effect). This study investigates this visual effect in combined visual–auditory integration with high- and low-variability speech and examines whether one’s prior tonal-language learning experience shapes the strength of this visual effect. Method: Thirty Mandarin-naïve listeners, 25 Mandarin second language learners, and 30 native Mandarin listeners...

Additional file 1: Table S1. Inclusionand exclusion criteria for subjects enrollment. Table S2. Characteristics of the validationcohort. Table S3. Relative abundance of 23 altered genera identified in SPgroup. Table S4. Relative abundance of 50 altered genera identified in AEgroup. Table S5. Significantly variant metabolicpathways identified in each group using LEfSe analyses. TableS6. LDA score values of 23 differential genera. Table S7. Eighthundred and thirty-five metabolites identified both from HC and SP group. Fig. S1. Correlationsbetween...

Abstract Background Emerging experimental and epidemiological evidence highlights a crucial cross-talk between the intestinal flora and the lungs, termed the “gut-lung axis”. However, the function of the gut microbiota in bronchiectasis remains undefined. In this study, we aimed to perform a multi-omics-based approach to identify the gut microbiome and metabolic profiles in patients with bronchiectasis. Methods Fecal samples collected from non-CF bronchiectasis patients (BE group, n = 61) and healthy volunteers (HC group, n =...

The burden of stomach cancer remains high in Hong Kong. We sought to evaluate the associations of age, period, and birth cohort with the changing trend in the incidence of stomach cancer and to provide projections through 2030. We performed an age-period-cohort analysis and projections up to 2030 using data from the Hong Kong Cancer Registry. Additionally, we used a population decomposition algorithm to assess the drivers in the number of incident cases of stomach...

Additional file 1. Supplement figures (Figs. S1–S15), materials and methods.