Data from: Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods

Josep Piñol, Gisela Mir, Priscila Gomez-Polo & Nuria Agusti
The quantification of the biological diversity in environmental samples using high-throughput DNA sequencing is hindered by the PCR bias caused by variable primer–template mismatches of the individual species. In some dietary studies, there is the added problem that samples are enriched with predator DNA, so often a predator-specific blocking oligonucleotide is used to alleviate the problem. However, specific blocking oligonucleotides could coblock nontarget species to some degree. Here, we accurately estimate the extent of the...

Registration Year

  • 2014
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Affiliations

  • Institute for Research and Technology in Food and Agriculture
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  • Centre for Research on Ecology and Forestry Applications
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  • Autonomous University of Barcelona
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