12 Works

Data from: Symbiont-mediated RNA interference in insects

Miranda M. A. Whitten, Paul D. Facey, Ricardo Del Sol, Lorena T. Fernandez, Meirwyn C. Evans, Jacob J. Mitchell, Owen G. Bodger & Paul J. Dyson
RNA interference (RNAi) methods for insects are often limited by problems with double-stranded (ds) RNA delivery, which restricts reverse genetics studies and the development of RNAi-based biocides. We therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, Rhodnius prolixus, and a short-lived globally invasive polyphagous agricultural pest, Western...

Data from: Combined analysis of variation in core, accessory and regulatory genome regions provides a super-resolution view into the evolution of bacterial populations

Alan McNally, Yaara Oren, Darren Kelly, Ben Pascoe, Steven Dunn, Tristan Seecharan, Minna Vehkala, Niko Välimäki, Michael B. Prentice, Amgad Ashour, Oren Avram, Tal Pupko, Ulrich Dobrindt, Ivan Literak, Sebastian Guenther, Katharina Schauffler, Lothar H. Wieler, Zong Zhiyong, Samuel K. Sheppard, James O. McInerney, Jukka Corander & Tristan Sreecharan
The use of whole-genome phylogenetic analysis has revolutionized our understanding of the evolution and spread of many important bacterial pathogens due to the high resolution view it provides. However, the majority of such analyses do not consider the potential role of accessory genes when inferring evolutionary trajectories. Moreover, the recently discovered importance of the switching of gene regulatory elements suggests that an exhaustive analysis, combining information from core and accessory genes with regulatory elements could...

Data from: A keystone mutualism underpins resilience of a coastal ecosystem to drought

Christine Angelini, John N. Griffin, Johan Van De Koppel, Leon P. M. Lamers, Alfons J. P. Smolders, Marlous Derksen-Hooijberg, Tjisse Van Der Heide & Brian R. Silliman
Droughts are increasing in severity and frequency, yet the mechanisms that strengthen ecosystem resilience to this stress remain poorly understood. Here, we test whether positive interactions in the form of a mutualism between mussels and dominant cordgrass in salt marshes enhance ecosystem resistance to and recovery from drought. Surveys spanning 250 km of southeastern US coastline reveal spatially dispersed mussel mounds increased cordgrass survival during severe drought by 5- to 25-times. Surveys and mussel addition...

Data from: Dynamic NF-κB and E2F interactions control the priority and timing of inflammatory signalling and cell proliferation

John M. Ankers, Raheela Awais, Nicholas A. Jones, James Boyd, Sheila Ryan, Anotony D. Adamson, Claire V. Harper, Lloyd Bridge, David G. Spiller, Dean A. Jackson, Pawel Paszek, Violaine Sée & Micheal R. H. White
Dynamic cellular systems reprogram gene expression to ensure appropriate cellular fate responses to specific extracellular cues. Here we demonstrate that the dynamics of Nuclear Factor kappa B (NF-κB) signalling and the cell cycle are prioritised differently depending on the timing of an inflammatory signal. Using iterative experimental and computational analyses, we show physical and functional interactions between NF-κB and the E2 Factor 1 (E2F-1) and E2 Factor 4 (E2F-4) cell cycle regulators. These interactions modulate...

Data from: Trait-matching and mass effect determine the functional response of herbivore communities to land use intensification

Gaëtane Le Provost, Nicolas Gross, Luca Börger, Hélène Deraison, Marilyn Roncoroni & Isabelle Badenhausser
1. Trait-based approaches represent a promising way to understand how trophic interactions shape animal communities. The approach relies on the identification of the traits that mediate the linkages between adjacent trophic levels, i.e. “trait-matching”. Yet, how trait-matching explains the abundance and diversity of animal communities has been barely explored. This question may be particularly critical in the context of land use intensification, currently threatening biodiversity and associated ecosystem services. 2. We collected a large dataset...

Data from: European sea bass show behavioural resilience to near-future ocean acidification

Mathieu Duteil, Edward C. Pope, Alfonso Perez-Escudero, Gonzalo G. De Polavieja, Ines Fürtbauer, M. R. Brown & Andrew J. King
Ocean acidification (OA)—caused by rising concentrations of carbon dioxide (CO2)—is thought to be a major threat to marine ecosystems and has been shown to induce behavioural alterations in fish. Here we show behavioural resilience to near-future OA in a commercially important and migratory marine finfish, the Sea bass (Dicentrarchus labrax). Sea bass were raised from eggs at 19°C in ambient or near-future OA (1000 µatm pCO2) conditions and n = 270 fish were observed 59–68...

Data from: Fine-scale flight strategies of gulls in urban airflows indicate risk and reward in city living

Emily L. C. Shepard, Cara Williamson & Shane P. Windsor
Birds modulate their flight paths in relation to regional and global airflows in order to reduce their travel costs. Birds should also respond to fine-scale airflows, although the incidence and value of this remains largely unknown. We resolved the three-dimensional trajectories of gulls flying along a built-up coastline, and used computational fluid dynamic models to examine how gulls reacted to airflows around buildings. Birds systematically altered their flight trajectories with wind conditions to exploit updraughts...

Data from: Fractal measures of spatial pattern as a heuristic for return rate in vegetative systems

Michael A. Irvine, Emma L. Jackson, Emma J. Kenyon, Kevan J. Cook, Matthew J. Keeling & James C. Bull
Measurement of population persistence is a long-standing problem in ecology; in particular, whether it is possible to gain insights into persistence without long time-series. Fractal measurements of spatial patterns, such as the Korcak exponent or boundary dimension, have been proposed as indicators of the persistence of underlying dynamics. Here we explore under what conditions a predictive relationship between fractal measures and persistence exists. We combine theoretical arguments with an aerial snapshot and time series from...

Data from: Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 response

William D. K. Reid, Andrew J. Close, Suzanne Humphrey, Gemma Chaloner, Lizeth Lacharme-Lora, Lisa Rothwell, Pete Kaiser, Nicola J. Williams, Thomas J. Humprey, Paul Wigley, Stephen P. Rushton & Tom J. Humphrey
Development of process orientated understanding of cytokine interactions within the gastrointestinal tract during an immune response to pathogens requires experimentation and statistical modelling. The immune response against pathogen challenge depends on the specific threat to the host. Here, we show that broiler chickens mount a breed-dependent immune response to Campylobacter jejuni infection in the caeca by analysing experimental data using frequentist and Bayesian structural equation models (SEM). SEM provides a framework by which cytokine interdependencies,...

Data from: The male handicap: male-biased mortality explains skewed sex ratios in brown trout embryos

Paloma Morán, Laurent Labbé & Carlos Garcia De Leaniz
Juvenile sex ratios are often assumed to be equal for many species with genetic sex determination, but this has rarely been tested in fish embryos due to their small size and absence of sex-specific markers. We artificially crossed three populations of brown trout and used a recently developed genetic marker for sexing the offspring of both pure and hybrid crosses. Sex ratios (SR = proportion of males) varied widely one month after hatching ranging from...

Data from: Fast life history traits promote invasion success in amphibians and reptiles

William L. Allen, Sally E. Street & Isabella Capellini
Competing theoretical models make different predictions on which life history strategies facilitate growth of small populations. While ‘fast’ strategies allow for rapid increase in population size and limit vulnerability to stochastic events, ‘slow’ strategies and bet-hedging may reduce variance in vital rates in response to stochasticity. We test these predictions using biological invasions since founder alien populations start small, compiling the largest dataset yet of global herpetological introductions and life history traits. Using state-of-the-art phylogenetic...

Data from: Biofilm morphotypes and population structure among Staphylococcus epidermidis from commensal and clinical samples

Llinos Harris, Susan Murray, Ben Pascoe, James Bray, Guillaume Meric, Leonardos Magerios, Thomas S. Wilkinson, Rose E. Jeeves, Holger Rohde, Stefan Schwarz, Herminia De Lencastre, Maria Miragaia, Joana Rolo, Rory Bowden, Keith A. Jolley, Martin C. J. Maiden, Dietrich Mack, Samuel Sheppard, Llinos G. Harris, Rose Jeeves & Samuel K. Sheppard
Bacterial species comprise related genotypes that can display divergent phenotypes with important clinical implications. Staphylococcus epidermidis is a common cause of nosocomial infections and, critical to its pathogenesis, is its ability to adhere and form biofilms on surfaces, thereby moderating the effect of the host’s immune response and antibiotics. Commensal S. epidermidis populations are thought to differ from those associated with disease in factors involved in adhesion and biofilm accumulation. We quantified the differences in...

Registration Year

  • 2016

Resource Types

  • Dataset


  • Swansea University
  • French National Institute for Agricultural Research
  • West China Hospital of Sichuan University
  • University of Sussex
  • University of Newcastle Australia
  • University of Hamburg
  • Duke University
  • Natural England
  • Cajal Institute
  • Radboud University Nijmegen