1,570 Works

Network pharmacology and in vitro experimental verification to explore the mechanism of Sanhua decoction in the treatment of ischaemic stroke

Wei Zhang, Li Zhang, Wen jun Wang, Shanbo Ma, Mingming Wang, Minna Yao, Ruili Li, Wei wei Li, Xian Zhao, Dongmei Hu, Yi Ding & Jingwen Wang
Stroke is an illness with high morbidity, disability and mortality that presents a major clinical challenge. Sanhua decoction (SHD) has been widely used to treat ischaemic stroke in the clinic. However, the potential mechanism of SHD remains unknown. To elucidate the multitarget mechanism of SHD in ischaemic stroke through network pharmacology and bioinformatics analyses. Network pharmacology and experimental validation approach was used to investigate the bioactive ingredients, critical targets and potential mechanisms of SHD against...

Bone marrow mesenchymal stem cells-derived exosomal microRNA-124-3p attenuates hypoxic-ischemic brain damage through depressing tumor necrosis factor receptor associated factor 6 in newborn rats

Weijie Min, Yina Wu, Yibin Fang, Bo Hong, Dongwei Dai, Yu Zhou, Jianmin Liu & Qiang Li
Mesenchymal stem cells (MSCs)-derived exosomes (Exo) are beneficial in the use of brain damages. Restrictively, the mechanism of Exo expressing miR-124-3p in hypoxic-ischemic brain damage (HIBD) is not completely comprehended. Thereupon, this work was put forward to reveal the action of bone marrow MSCs-derived Exo (BMSCs-Exo) expressing miR-124-3p in the illness. BMSCs were isolated and transfected with miR-124-3p agomir. Then, BMSCs-Exo were extracted and identified. The newborn HIBD rats were injected with miR-124-3p-modified BMSCs-Exo or...

Bone marrow mesenchymal stem cells-derived exosomal microRNA-124-3p attenuates hypoxic-ischemic brain damage through depressing tumor necrosis factor receptor associated factor 6 in newborn rats

Weijie Min, Yina Wu, Yibin Fang, Bo Hong, Dongwei Dai, Yu Zhou, Jianmin Liu & Qiang Li
Mesenchymal stem cells (MSCs)-derived exosomes (Exo) are beneficial in the use of brain damages. Restrictively, the mechanism of Exo expressing miR-124-3p in hypoxic-ischemic brain damage (HIBD) is not completely comprehended. Thereupon, this work was put forward to reveal the action of bone marrow MSCs-derived Exo (BMSCs-Exo) expressing miR-124-3p in the illness. BMSCs were isolated and transfected with miR-124-3p agomir. Then, BMSCs-Exo were extracted and identified. The newborn HIBD rats were injected with miR-124-3p-modified BMSCs-Exo or...

Network pharmacology and in vitro experimental verification to explore the mechanism of Sanhua decoction in the treatment of ischaemic stroke

Wei Zhang, Li Zhang, Wen jun Wang, Shanbo Ma, Mingming Wang, Minna Yao, Ruili Li, Wei wei Li, Xian Zhao, Dongmei Hu, Yi Ding & Jingwen Wang
Stroke is an illness with high morbidity, disability and mortality that presents a major clinical challenge. Sanhua decoction (SHD) has been widely used to treat ischaemic stroke in the clinic. However, the potential mechanism of SHD remains unknown. To elucidate the multitarget mechanism of SHD in ischaemic stroke through network pharmacology and bioinformatics analyses. Network pharmacology and experimental validation approach was used to investigate the bioactive ingredients, critical targets and potential mechanisms of SHD against...

Ultrasound targeted microbubble destruction-mediated SOCS3 attenuates biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells

Xiaojiang Tang, Na Hao, Yuhui Zhou & Yang Liu
SOCS3 is low-expressed in breast cancer and may be a potential target. Ultrasound targeted microbubble destruction (UTMD) improved the efficiency of gene transfection. We explored the effects of UTMD-mediated transfection of SOCS3 on the biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells (BCSCs). The expression of SOCS3 in breast cancer (BC) and its association with prognosis were evaluated by GEPIA and The Cancer Genome Atlas (TCGA) websites. BCSCs were sorted by flow...

Risk factors influencing cure of ultrasound-guided microwave ablation for primary hyperparathyroidism

Fangyi Liu, Li Zang, Yang Liu, Xiaoling Yu, Zhigang Cheng, Zhiyu Han, Jie Yu & Ping Liang
To evaluate the potential risk factors influencing cure rate of ultrasound-guided microwave ablation (MWA) for primary hyperparathyroidism (PHPT). Seventy five patients (25 males and 50 females; mean age, 56.80 ± 12.34; age range, 26–85) with PHPT undergoing MWA under ultrasound guidance were enrolled between May 2017 and December 2020. The cure rate and complications were evaluated after treatment. The potential factors influencing cure rate of ultrasound-guided MWA for PHPT were analyzed by univariate and multivariate...

Combined radiofrequency ablation or microwave ablation with transarterial chemoembolization can increase efficiency in intermediate-stage hepatocellular carcinoma without more complication: a systematic review and meta-analysis

Yao Yang, Hongli Yu, Lingyu Qi, Chenxi Liu, Yuemin Feng, Jianni Qi, Jie Li & Qiang Zhu
Radiofrequency ablation (RFA) and microwave ablation (MWA) are widely used in combination with transarterial chemoembolization (TACE) for intermediate-stage hepatocellular carcinoma (HCC) in clinical practice. We aim to compare the efficacy and safety of TACE combined with RFA or MWA versus TACE monotherapy for intermediate-stage HCC. We searched PubMed, Embase, Web of Science, and the Cochrane Library for relevant studies. The outcomes included overall survival (OS), progression-free survival (PFS), local tumor control (LTC) rate, and major...

Effects of glycerol and glucose on docosahexaenoic acid synthesis in Aurantiochyrium limacinum SFD-1502 by transcriptome analysis

Huaqiu Zhang, Xiangying Zhao, Chen Zhao, Jiaxiang Zhang, Yang Liu, Mingjing Yao & Jianjun Liu
Docosahexaenoic acid (DHA) has numerous functions in adjusting the organic health and pragmatic value in medicine and food field. In this study, we compared glycerol and glucose as the only carbon source for DHA production by Aurantiochytrium. When the glycerol concentration was 120 g/L, the maximum DHA yield was 11.08 g/L, and the DHA yield increased significantly, reaching 47.67% of the total lipid content. When the cells grew in glucose, the DHA proportion was 37.39%....

Identification and potential mechanism of different components from the aerial part of Bupleurum chinense DC. for epileptic treatment

Xiaomao Li, Yan Liu, Siyi Wang, Wei Guan, Juan Pan, Haixue Kuang & Bingyou Yang
The present study aimed to assess the effects of epileptic treatment of the aerial part of Bupleurum chinense DC. in kainic acid (KA)-induced epilepsy rats and LPS-induced BV2 cells, as well as to identify the active chemical constituents. The in vivo and vitro results showed that 20% ethanol elution fractions of the aerial part of B. chinense DC. (BCE-20) and 70% ethanol elution fractions of the aerial part of B. chinense DC. (BCE-70) could improve...

UHPLC-QTOF-MS/MS based characterization of anti-tumor constituents in Ceratocarpus arenarius L. and identification of EGFR-TK inhibitors by virtual screening

Le Pan, Li Li, Lu Xu, Jiaojiao Zhang, Jiashan Li, Ming Gao, JinMen Yu, Lu Jin & Dongyu Lei
The constituents of Ceratocarpus arenarius L., as a traditional anticancer medicine of Kazakh, were firstly profiled with UHPLC-QTOF-MS/MS. The potential compounds against EGFR-TK were virtually screened. As a result, forty-four compounds were analyzed, including 18 flavonoids, 8 steroids, 4 phenolic acids, 9 fatty acids, 1 coumarin and 4 other compounds. Among them, 9 flavonoids, N-trans-Feruloyltyramine (5), stigmasterol (11) and carthamone (38) were recognized as potential key anti-tumor constituents of C. arenarius through docking to active...

Genome-wide identification and expression pattern analysis of the TCP transcription factor family in Ginkgo biloba

Li Yu, Qiangwen Chen, Jiarui Zheng, Feng Xu, Jiabao Ye, Weiwei Zhang, Yongling Liao & Xiaoyan Yang
Plant-specific TCP transcription factors play an essential role in plant growth and development. They can regulate leaf curvature, flower symmetry and the synthesis of secondary metabolites. The flavonoids in Ginkgo biloba leaf are one of the main medicinally bioactivate compounds, which have pharmacological and beneficial health effects for humans. In this study, a total of 13 TCP genes were identified in G. biloba, and 5 of them belonged to PCF subclades (GbTCP03, GbTCP07, GbTCP05, GbTCP13,...

An overview of the methods for analyzing the chemical forms of metals in plants

Jiawei Yang, Lin Sun, Xing Shen, Min Dai, Imran Ali, Changsheng Peng & Iffat Naz
Currently, the occurrence of toxic levels of metals in soils is a serious environmental issue worldwide. Phytoremediation is getting much attention to control metals soil pollution because it is economic and environmentally friendly. However, the methods used to detect metals in plants are not uniform and have depicted poor comparability of the research investigations. Therefore, the present overview is designed to discuss the possible chemical forms of metals in various environmental matrixes and the detection...

Regulation of microRNA miR-197-3p/CDC28 protein kinase regulatory subunit 1B (CKS1B) axis by Circular RNA hsa_circ_0000285 promotes glioma progression

Tao Deng, Yang Liu, Yanlong Yang, Leyong Yuan, Fangfang Liu, Xiaobo Wang, Qiuying Zhang & Mingshui Xie
Circular RNA circ_0000285 is differentially expressed in several malignancies; however, its role in gliomas is under investigation. Reverse transcription quantitative polymerase chain reaction was conducted to evaluate the expression of circ_0000285, miR-197-3p, and CDC28 protein kinase regulatory subunit 1B (CKS1B) in glioma tissues and cells. Cell Counting Kit-8 and Transwell invasion assays coupled with Western blotting analysis using anti-Bax and anti-Bcl-2 antibodies were performed to evaluate cell proliferation, invasion, and apoptosis. Luciferase reporter and AGO2...

Stocking density affects transcriptome changes in the hypothalamic-pituitary-gonadal axis and reproductive performance in ducks

Hehe Liu, Xia Xiong, Fajun Pu, Jianmei Wang, Yanying Li, Yang Xi, Shengchao Ma, Lili Bai, Rongping Zhang, Li Liang & Chaowu Yang
The hypothalamic-pituitary-adrenal (HPA) axis and hypothalamic-pituitary-gonadal (HPG) axis plays a central role in mediating physiological responses related to the reproductive system under any stressful condition. However, the molecular mechanism underlying the effects of stress on physiology still needs to be elucidated. This study demonstrated that increasing the stocking density from 4 to 8 birds/m2 during the laying period decreased the egg production rate of laying ducks by 13.04 − 63.55% and feed intake by 7.40...

Determination of Glucose by the Catalysis of Luminol Chemiluminescence Using One-Step Synthesized Platinum/Silver Nanoparticles as a Peroxidase Mimetic

Miaomiao Tian, Liping Zhao, Ya Wang, Gang Liu & Peng Zhang
Bimetallic Pt/Ag nanoparticles (Pt/Ag NPs) were synthesized using 3-hydroxyphenyl-boronic acid as the reducing and stabilizing agent by a simple, one-step hydrothermal method. The bimetallic Pt/Ag NPs were demonstrated to possess intrinsic peroxidase mimetic catalytic activity for the luminol/H2O2 reaction to generate chemiluminescence (CL). The catalytic properties of the bimetallic Pt/Ag NPs were thoroughly investigated. The results showed that the combination of Pt and Ag greatly enhanced the chemiluminescence which is attributed to their synergy. Moreover,...

Determination of Glucose by the Catalysis of Luminol Chemiluminescence Using One-Step Synthesized Platinum/Silver Nanoparticles as a Peroxidase Mimetic

Miaomiao Tian, Liping Zhao, Ya Wang, Gang Liu & Peng Zhang
Bimetallic Pt/Ag nanoparticles (Pt/Ag NPs) were synthesized using 3-hydroxyphenyl-boronic acid as the reducing and stabilizing agent by a simple, one-step hydrothermal method. The bimetallic Pt/Ag NPs were demonstrated to possess intrinsic peroxidase mimetic catalytic activity for the luminol/H2O2 reaction to generate chemiluminescence (CL). The catalytic properties of the bimetallic Pt/Ag NPs were thoroughly investigated. The results showed that the combination of Pt and Ag greatly enhanced the chemiluminescence which is attributed to their synergy. Moreover,...

NLRC5 restricts dengue virus infection by promoting the autophagic degradation of viral NS3 through E3 ligase CUL2 (cullin 2)

Jiawei Hao, Jinqian Li, Zhenzhen Zhang, Yang Yang, Qing Zhou, Tiantian Wu, Tongling Chen, Zhongdao Wu, Ping Zhang, Jun Cui & Yi-Ping Li
NLRC5 has been reported to be involved in antiviral immunity; however, the underlying mechanism remains poorly understood. Here, we investigated the functional role of NLRC5 in the infection of a flavivirus, dengue virus (DENV). We found that the expression of NLRC5 was strongly induced by virus infection and IFNB or IFNG stimulation in different cell lines. Overexpression of NLRC5 remarkably suppressed DENV infection, whereas knockout of NLRC5 led to a significant increase in DENV infection....

Research strategy for constructing a green infrastructure network based on spatial prioritization

Dongmeng Wang, Chang Liu, Yue Guo, Puxia Tang, Jie Jiao, Weihan Kong, Ling Zhang, Yiping Liu & Dezheng Kong
The destruction of the ecological system caused by urban expansion has led to the environmental deterioration, cities have become increasingly vulnerable. In this study, six districts and counties along the Yellow River in Zhengzhou were selected as the study area. First, green infrastructure elements were extracted by morphological spatial pattern analysis. Then, outside the urban areas, we used connectivity analysis to evaluate the importance of core areas, adopted minimum cumulative resistance model to extract potential...

Design, synthesis, and evaluation of novel O-alkyl ferulamide derivatives as multifunctional ligands for treating Alzheimer’s disease

Gaofeng Zhu, Ping Bai, Keren Wang, Jing Mi, Jing Yang, Jiaqi Hu, Yujuan Ban, Ran Xu, Rui Chen, Changning Wang, Lei Tang & Zhipei Sang
Herein, a series of novel O-alkyl ferulamide derivatives were designed and synthesised through the multi-target-directed ligands (MTDLs) strategy. The biological activities in vitro showed that compounds 5a, 5d, 5e, 5f, and 5h indicated significantly selective MAO-B inhibitory potency (IC50 = 0.32, 0.56, 0.54, 0.73, and 0.86 μM, respectively) and moderate antioxidant activity. Moreover, compounds 5a, 5d, 5e, 5f, and 5h showed potent anti-inflammatory properties, remarkable effects on self-induced Aβ1-42 aggregation, and potent neuroprotective effect on...

Additional file 7 of Study on the prognosis, immune and drug resistance of m6A-related genes in lung cancer

Yang Yang, Zhouyao Qian, Mingyang Feng, Weiting Liao, Qiuji Wu, Feng Wen & Qiu Li
Additional file 7. Infiltration of gene expression, overall survival, mutation profile and the comparison of tumor infiltration levels in m6A-related genes with different somatic copy number alterations for LUAD and LUSC in TIMER2.0.

Study on the prognosis, immune and drug resistance of m6A-related genes in lung cancer

Yang Yang, Zhouyao Qian, Mingyang Feng, Weiting Liao, Qiuji Wu, Feng Wen & Qiu Li
Abstract Background Few studies have demonstrated that the relationship between m6A-related genes and the prognosis, tumor microenvironment and drug resistance of LC. Methods The main results were analyzed with bioinformatics methods. Results Hence, we found 10 m6A-related genes expressed less in tumor samples in comparison with normal ones. Using consensus clustering, all LC patients were grouped into 2 subgroups according to the overall expression of 10 differential expressed m6A-related genes. In two clusters, the OS...

Additional file 1 of Application of pedicled greater omentum flap tamponade combined with laparoscopic fenestration in diaphragmatic hepatic cyst

Zujian Wu, Yun Chen, Ye Jin, Changfeng Liu, Yusu Liu & Bing Zhang
Supplementary Material 1: Video

Survival comparison of different histological subtypes of oropharyngeal squamous cell carcinoma: A propensity-matched score analysis based on SEER database

Tao Li, Yi Wang, Xianwang Xiang & Chuanjun Chen
ObjectiveThe purpose of this study was to analyze the difference of survival rates in paitents with oropharyngeal keratinizing squamous cell carcinoma (KSCC), nonkeratinizing squamous cell carcinoma (NKSCC), basaloid squamous cell carcinoma (BSCC), and papillary squamous cell carcinoma (PSCC).Materials and methodsPatients diagnosed with oropharyngeal squamous cell carcinoma between 2004 and 2015 were collected from the SEER database. Cox proportional hazards models and Kaplan–Meier curves were used for survival analysis. Propensity score matching (PSM) was performed to...

Additional file 3 of Intrauterine growth retardation affects liver bile acid metabolism in growing pigs: effects associated with the changes of colonic bile acid derivatives

Yang Liu, Md. Abul Kalam Azad, Wanghong Zhang, Liang Xiong, Francois Blachier, Zugong Yu & Xiangfeng Kong
Additional file 3: Table S3. The composition and nutrient levels of pigs’ diets (as-fed basis).

Long non-coding RNA SNHG16, binding with miR-106b-5p, promoted cell apoptosis and inflammation in allergic rhinitis by up-regulating leukemia inhibitory factor to activate the JAK1/STAT3 signaling pathway

Huajing Li, Fang Quan, Pengfei Zhang & Yuan Shao
Allergic rhinitis (AR) is a type I hypersensitive disease. Long non-coding RNA (lncRNA) SNHG16 acts as an oncogene in a variety of tumors and promotes the occurrence of inflammation in many inflammatory diseases. The study aims to investigate the expression of SNHG16 and its potential biological functions in AR. RT-qPCR results showed that the expression of SNHG16 in AR was up-regulated. The AR cell model was constructed by stimulating primary nasal mucosal epithelial cells from...

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  • Second Xiangya Hospital of Central South University
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  • Chinese Academy of Sciences
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  • Chinese Academy of Medical Sciences & Peking Union Medical College
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