Data from: Does hybridization with a widespread congener threaten the long-term persistence of the Eastern Alpine rare local endemic Knautia carinthiaca?Martin Čertner, Filip Kolář, Peter Schönswetter & Božo Frajman
Interspecific hybridization, especially when regularly followed by backcrossing (i.e., introgressive hybridization), conveys a substantial risk for many endangered organisms. This is particularly true for narrow endemics occurring within distributional ranges of widespread congeners. An excellent example is provided by the plant genus Knautia (Caprifoliaceae): Locally endemic K. carinthiaca is reported from two isolated populations in southern Austria situated within an area predominantly occupied by widespread K. arvensis. While K. carinthiaca usually inhabits low-competition communities on...
Diet analysis is an important aspect when investigating the ecology of fish-eating animals and essential for assessing their functional role in food webs across aquatic and terrestrial ecosystems. The identification of fish remains in dietary samples, however, can be time-consuming and unsatisfying using conventional morphological analysis of prey remains. Here, we present a two-step multiplex PCR system, comprised of six assays, allowing for rapid, sensitive and specific detection of fish DNA in dietary samples. This...
Data from: \"You are not what you eat: massive parallel sequencing reveals that gut microbiome is not diet-related in larval Dilophus febrilis (Diptera: Bibionidae)\" in Genomic Resources Notes Accepted 1 June 2015 to 31 July 2015Alexander Rief, Wolfgang Arthofer, Birgit C. Schlick-Steiner, Florian M. Steiner & Julia Seeber
This article documents the public availability of metagenome sequence data from 454 amplicon sequencing of larval dipteran gut (Dilophus febrilis) and their potential food sources dwarf shrub litter (Vaccinium gaultheroides), grass litter (Dactylis glomerata), and cow dung (Bos primigenius taurus).
Data from: Ecological differentiation, lack of hybrids involving diploids, and asymmetric gene flow between polyploids in narrow contact zones of Senecio carniolicus (syn. Jacobaea carniolica, Asteraceae)Karl Hülber, Michaela Sonnleitner, Jan Suda, Jana Krejčíková, Peter Schönswetter, Gerald M. Schneeweiss & Manuela Winkler
Areas of immediate contact of different cytotypes offer a unique opportunity to study evolutionary dynamics within heteroploid species and to assess isolation mechanisms governing coexistence of cytotypes of different ploidy. The degree of reproductive isolation of cytotypes, i.e., the frequency of heteroploid crosses and subsequent formation of viable and (partly) fertile hybrids, plays a crucial role for the long-term integrity of lineages in contact zones. Here, we assessed fine-scale distribution, spatial clustering and ecological niches...
Data from: \"Discovery and characterization of 80 SNPs and 1,624 SSRs in the transcriptome of Atlantic mackerel (Scomber scombrus, L)\" in Genomic Resources Notes Accepted 1 June 2015 to 31 July 2015Iratxe Montes, Jorge Langa, César Vilas, Sarah J. Helyar, Paula Álvarez, Darrell Conklin & Andone Estonba
This paper reports on SNP discovery in the Atlantic mackerel transcriptome, using next generation sequencing technologies and applying developed methodology already proven successful for the European anchovy. A total of 9,966 high quality transcriptome contigs were assembled, from which 951 putative SNPs were discovered. In all, 479 putative SNPs and 1,624 simple sequence repeats (SSRs) suitable for genotyping were identified. A subset of 96 was selected for genotyping; from these, 80 SNPs were considered polymorphic...
Data from: Larval UV exposure impairs adult immune function through a trade-off with larval investment in cuticular melaninSara Debecker, Ruben Sommaruga, Tim Maes & Robby Stoks
Despite the strong impact of ultraviolet (UV) radiation on invertebrates, it is unknown whether it affects immune function across metamorphosis. More generally, the mechanisms on how larval stressors bridge metamorphosis and shape adult fitness in animals with a complex life cycle remain poorly understood. We studied whether cuticular melanin content is upregulated under UV exposure in the larval stage of the damselfly Coenagrion puella and whether this is traded off across metamorphosis against a key...
Data from: \"Development of the draft genome sequence of the marbled flounder Pseudopleuronectes yokohamae as a reference of population genomic analysis\" in Genomic Resources Notes accepted 1 February 2015 – 31 March 2015Yuki Minegishi, Minoru Ikeda, Lynda F. Delph, Laura A. Weingartner, Peter D. Fields, Laura Bertini, Carla Caruso, Silvia Proietti, Wolfgang Arthofer, Francesco Cicconardi, Heike Ritthammer, Birgit C. Schlick-Steiner, Florian M. Steiner, Gregor A. Wachter & Herbert C. Wagner
This article documents the public availability of the draft genome sequence data (raw reads, assembled contigs and unassembled reads) and RAD-tag read data of the marbled flounder Pseudopleuronectes yokohamae (Pleuronectiformes; Pleuronectidae).
Data from: Polyploidisation and geographic differentiation drive diversification in a European high mountain plant group (Doronicum clusii aggregate, Asteraceae)Clemens Pachschwöll, Pedro Escobar García, Manuela Winkler, Gerald M. Schneeweiss & Peter Schönswetter
Range shifts (especially during the Pleistocene), polyploidisation and hybridization are major factors affecting high-mountain biodiversity. A good system to study their role in the European high mountains is the Doronicum clusii aggregate (Asteraceae), whose four taxa (D. clusii s.s., D. stiriacum, D. glaciale subsp. glaciale and D. glaciale subsp. calcareum) are differentiated geographically, ecologically (basiphilous versus silicicolous) and/or via their ploidy levels (diploid versus tetraploid). Here, we use DNA sequences (three plastid and one nuclear...
Data from: \"De novo assembled transcriptome of organs involved in reproduction in an endangered endemic Iberian cyprinid fish (Squalius pyrenaicus)\" in Genomic Resources Notes Accepted 1 June 2015 to 31 July 2015Miguel P. Machado, Joana Pinho, Ana R. Grosso, Manfred Schartl, Maria M. Coelho, C. Vilas, Wolfgang Arthofer, Alexander Rief, Birgit C. Schlick-Steiner, Julia Seeber, Florian M. Steiner, D. Conklin, A. Estonba, J. Langa & I. Montes
Sex determination systems are diverse, especially among fish, and include genetic and/or environmental components. Unexpectedly for such a basic aspect of development, sex determination systems change rapidly during evolution and gonadal fate is not ultimate, being actively maintained lifelong. Here, sequences of expressed genes involved in maintenance of gonad identity and reproduction processes were obtained through transcriptome assembly of the brain-gonadal axis tissues of a freshwater fish inhabiting highly variable environments, the gonochoristic Iberian fish...
Data from: \"A reduced representation libraries approach for nuclear marker development via 454 sequencing applied on Tetramorium (Hymenoptera: Formicidae)\" in Genomic Resources Notes Accepted 1 February 2015 to 31 March 2015Herbert C. Wagner, Francesco Cicconardi, Gregor A. Wachter, Heike Ritthammer, Florian M. Steiner, Birgit C. Schlick-Steiner & Wolfgang Arthofer
To clarify species boundaries in the cryptic Tetramorium caespitum/impurum ant species complex, a multi-disciplinary approach is necessary. Nuclear DNA markers constitute one important step in species delimitation. We established a reduced representation library (RRL) based on amplified fragment-length polymorphism (AFLP) PCR products of four species. Amplicons were size-selected by gel-electrophoresis, DNA-fragments recovered from the agarose gels, and 454 libraries constructed by a commercial provider. Pyrosequencing yielded 490,155 reads, from which we designed cross-species amplifying primer...
Data from: Taking the discovery approach in integrative taxonomy: decrypting a complex of narrow-endemic Alpine harvestmen (Opiliones: Phalangiidae: Megabunus)Gregor A. Wachter, Christoph Muster, Wolfgang Arthofer, Günther Raspotnig, Petra Föttinger, Christian Komposch, Florian M. Steiner & Birgit C. Schlick-Steiner
Species delimitation is fundamental for biological studies, yet precise delimitation is not an easy task, and every involved approach has an inherent failure rate. Integrative taxonomy, a method that merges multiple lines of evidence, can profoundly contribute to reliable alpha taxonomy and shed light on the processes behind speciation. In this study, we explored and validated species limits in a group of closely related Megabunus harvestmen (Eupnoi, Phalangiidae) endemic to the European Alps. Without a...
Data from: \"Transcriptome sequencing of the Antarctic Colobanthus quitensis (Kunth) Bartl (Caryophillaceae)\" in Genomic Resources Notes Accepted 1 February 2015 – 31 March 2015Laura Bertini, Silvia Proietti & Carla Caruso
This article documents the public availability of raw transcriptome sequence data and assembled contigs of the Antarctic plant Colobanthus quitensis grown in two different climatic conditions. BLAST hits are also provided.
University of Innsbruck12
University of the Basque Country3
Indiana University Bloomington3
Andalusian Institute of Agricultural and Fisheries Research and Training3
University of Würzburg2
University of Lisbon2
University of Vienna2
University of Natural Resources and Life Sciences2