Long non-coding RNAs (lncRNAs) are involved in developing hepatocellular carcinoma (HCC). The present study explored the role of lncRNA LINC01194, which is upregulated in HCC tissues and might be a vital regulator in HCC progression. Levels of LINC01194, microRNA (miR)-655-3p, and SMAD family member 5 (SMAD5) were assessed using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The bioactivity of Huh-7 cells was assessed using cell counting kit-8 and transwell assays and flow cytometry. Western...

To investigate the functions of hexokinase 1 (HK1) in the formation of goose fatty liver, a total of 40 healthy 63-day-old male Landes geese were selected and randomly assigned to a control group and an overfeeding treatment. In addition, the overexpression or RNA interference assay of HK1, and transcriptome analysis after HK1 overexpression were performed in the goose primary hepatocytes. Data showed that the mRNA expression of hepatic HK1 was upregulated in overfed treatment compared...

To investigate the functions of hexokinase 1 (HK1) in the formation of goose fatty liver, a total of 40 healthy 63-day-old male Landes geese were selected and randomly assigned to a control group and an overfeeding treatment. In addition, the overexpression or RNA interference assay of HK1, and transcriptome analysis after HK1 overexpression were performed in the goose primary hepatocytes. Data showed that the mRNA expression of hepatic HK1 was upregulated in overfed treatment compared...

Transition metal oxide was considered as an effective cathode additive to depress ‘shuttle effect’ in lithium-sulfur (Li-S) batteries via forming a strong polar chemical bond with polysulfides. Here, we firstly synthesized unique S@Ni/NiO/ZnSnO3 microcubes as composite cathodes for Li-S batteries, in which Ni/NiO films were deposited onto surface of as-prepared ZnSnO3 microcubes via a hydrothermal reaction and later calcinations process. The S@Ni/NiO/ZnSnO3 composite showed attractive electrochemical performances with a high initial discharge-specific capacity of 1070...

Accumulating evidence indicates that N6-methyladenosine (m6A) and long noncoding RNAs (lncRNAs) play crucial roles in cancer development. However, the biological roles of m6A and lncRNAs in lung cancer tumorigenesis are largely unknown. In this study, SVIL antisense RNA 1 (SVIL-AS1) was downregulated in lung adenocarcinoma (LUAD) tissues and was associated with a favorable prognosis in patients with LUAD. SVIL-AS1 overexpression suppressed LUAD cell proliferation and blocked cell cycle arrest. Mechanistically, METTL3 increased the m6A modification...

SOCS3 is low-expressed in breast cancer and may be a potential target. Ultrasound targeted microbubble destruction (UTMD) improved the efficiency of gene transfection. We explored the effects of UTMD-mediated transfection of SOCS3 on the biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells (BCSCs). The expression of SOCS3 in breast cancer (BC) and its association with prognosis were evaluated by GEPIA and The Cancer Genome Atlas (TCGA) websites. BCSCs were sorted by flow...

In the present study, we aimed to investigate the role of long non-coding RNA terminal differentiation-induced non-coding RNA (TINCR) in cisplatin (DDP) resistance of choroidal melanoma (CM) and the potential molecular mechanisms. CM and non-CM tissues were collected from 60 CM patients. DDP-resistant CM cells were obtained by selection with linearly increased DDP treatment. The expression levels of TINCR, microR-19b-3p (miR-19b-3p), and extracellular signal-regulated kinase 2 (ERK-2) were detected by quantitative real-time PCR. Cholecystokinin octapeptide...

Endometrial carcinoma (EC) originates from the endometrium and is one of the most common tumors in female patients, and its incidence has continued to increase in recent decades. LncRNAs are involved in the pathogenesis and metastasis of a variety of malignant tumors, which indicates that lncRNAs can be used as tumor diagnostic markers and potential therapeutic targets. In this study, we analyzed the RNA transcripts of EC cells from The Cancer Genome Atlas (TCGA) and...

Endometrial carcinoma (EC) originates from the endometrium and is one of the most common tumors in female patients, and its incidence has continued to increase in recent decades. LncRNAs are involved in the pathogenesis and metastasis of a variety of malignant tumors, which indicates that lncRNAs can be used as tumor diagnostic markers and potential therapeutic targets. In this study, we analyzed the RNA transcripts of EC cells from The Cancer Genome Atlas (TCGA) and...

The nutritional components and protein quality of genetically modified maize expressing phytase gene (GM) were analyzed and evaluated in this study. The nutritional components were analyzed by Chinese national standard methods. The ileostomy Bama miniature pigs were utilized to analyze the true digestibility of protein and amino acids. The digestible indispensable amino acid score (DIAAS) was adopted to evaluate the protein quality of GM, its parental maize (PM) and commercial available maize Zhengdan 958 (ZD)....

Sustained cardiac hypertrophy (CH) contributes to many heart diseases. Long noncoding RNAs (lncRNAs) collectively play critical roles in cardiovascular diseases (CVDs). However, the roles of lncRNA H19 in CH are still unclear. A CH model was constructed utilizing isoproterenol (ISO). We demonstrated H19 could participate in regulating ISO-induced CH development both in vivo and in vitro. The online databases DIANA and TargetScan were used to predict the targets of H19 and MicroRNA-145-3p (miR-145-3p), respectively. Luciferase...

Diabetic kidney disease (DKD) is a serious diabetes complication. Sodium-glucose cotransporter 2 inhibitors (SGLT2i) are novel anti-diabetes drugs that confer clinical renal protection. However, the molecular mechanisms involved remain unclear. Here, human proximal tubular epithelial cells (PTECs) were treated with normal glucose, high glucose, and anti-diabetes agents, including SGLT2i (dapagliflozin), metformin, and dipeptidyl peptidase-4 inhibitor (DPP-4i, vildagliptin) and microarray analysis was performed. Firstly, a total of 2,710 differentially expressed circular RNAs (circRNAs) were identified. Secondly,...

Additional file 1. Supplementary Material for a Successful Hybrid Deep Learning Model aiming at Promoter Identification.

Organic contaminants of emerging concern (OCECs) originating from assorted sources have attracted increasing attention latterly as most of them are not under routine surveillance or effective regulation but possess persistent, bioaccumulative, and toxic potential. Massive terrestrial inputs and proximity to intense human activities have elevated OCEC contamination in many estuaries. The present review outlines the current knowledge on the environmental occurrence and fate, as well as bioavailability of nine classes of OCECs, i.e., per- and...

1. Melatonin is an indole hormone that, among its myriad biological functions, regulates circadian and seasonal rhythms in animals. The ASMT gene plays an essential role in melatonin synthesis. However, in chickens, little is known about the regulatory elements governing its transcription. 2. The following study identified the transcription start site of the chicken ASMT gene by 5’-RACE. Then, the proximal minimal promoter was identified using a series of 5′ truncations of the ASMT promoter...

Primary ovarian insufficiency (POI), also known as premature ovarian failure, is an ovarian defect in humans characterized by the premature depletion of ovarian follicles before the age of 40. However, the mechanisms underlying POI remain largely unknown. Here, we show that knockout of Epg5 (ectopic P-granules autophagy protein 5 homolog (C. elegans)) results in subfertility in female mice, which exhibit a POI-like phenotype. Single-cell RNA sequencing analysis revealed that the knockout of Epg5 affected the...

Additional file 1: Supplementary Figure 1. Funnel plot in themeta-analysis on the association of waist circumference between prematureovarian insufficiency and control group. Supplementary Figure 2. Funnel plot in the meta-analysis on the association of systolic blood pressure betweenpremature ovarian insufficiency and control group. Supplementary Figure 3. Funnel plot in the meta-analysis on the association of diastolic blood pressurebetween premature ovarian insufficiency and control group. Supplementary Figure 4. Funnel plot in the meta-analysis on the association...

Additional file 2: Supplementary table 1. PRISMA 2022 checklist.

Abstract Background Oxidative stress is a highly active metabolic process in the liver, that poses great threats to disseminated tumor cells during their colonization. Here, we aimed to investigate how colorectal cancer (CRC) cells overcome lipid peroxidation to sustain their metastatic colonization in the liver. Methods Orthotopic colorectal liver metastasis (CRLM) and CRC liver colonization mouse models were constructed to determine the roles of lipid peroxidation and AADAC in CRC liver colonization. The levels of...

TableS1. Clinical data of ovarian cancer patients from TCGA and ICGC.
TableS2.Identified DNA driver methylation (DNAme) in TCGA, ICGC and GSE146556.
TableS3. The correlation between DNAme and corresponding mRNA expression in TCGA, ICGC and GSE146556. TableS4.Analysis of pathway enrichment (KEGG) and Gene Ontology (GO) of DNAme-associated mRNA in TCGA.
TableS5. Univariate Cox regression analysis of DNAme-associated mRNA. (P < 0.05)
TableS6. Analysis of pathway enrichment (KEGG) and Gene Ontology (GO) of...

Supplemental material, sj-docx-1-tct-10.1177_15330338221124658 for In Vitro and in Vivo Study of the Effect of Osteogenic Pulsed Electromagnetic Fields on Breast and Lung Cancer Cells by Mike Y. Chen, Jing Li, Nianli Zhang and Erik I. Waldorff, James T. Ryaby, Philip Fedor, Yongsheng Jia, Yujun Wang in Technology in Cancer Research & Treatment

Supplemental material, sj-xlsx-5-tct-10.1177_15330338221124658 for In Vitro and in Vivo Study of the Effect of Osteogenic Pulsed Electromagnetic Fields on Breast and Lung Cancer Cells by Mike Y. Chen, Jing Li, Nianli Zhang and Erik I. Waldorff, James T. Ryaby, Philip Fedor, Yongsheng Jia, Yujun Wang in Technology in Cancer Research & Treatment

Additional file 1: Table S1. Baseline characteristics and cardiovascular events in the participants who visited the hospital at least twice. Table S2. Differences in demographic characteristics among the groups stratified by levels of fasting C-peptide for the participants without previous T2DM. Table S3. Sensitivity analyses of the associations stratified by glucose metabolism status and tertiles of HOMA-IR for the participants without previous T2DM. Table S4. Prevalence of cardiovascular events in the participants stratified by quantiles...

Additional file 1: Figure S1. The relationship between a healthy lifestyle and CMM in subgroup analyses. Figure S2. The relationship between a healthy lifestyle and CMM in seven sensitivity analyses. Figure S3. The frequency distribution graph of the weighted healthy lifestyle score for the whole population. Figure S4. The frequency distribution graph of the weighted healthy lifestyle score for 2/3 of the population. Figure S5. The frequency distribution graph of the sum healthy lifestyle score...

Additional file 2. Table S1. Ubiquitination annotation. Table S2. Protein annotation. Table S3. 42 proteins having ten or more than ten ubiquitination modifications. Table S4. 20 ubiquitin-binding motifs. Table S5. 2242 proteins with an upregulated ubiquitination and 1204 proteins with a down-regulated ubiquitination. Table S6. 1172 up-regulated proteins and 1700 down-regulated proteins. Table S7. 646 Secretory proteins. Table S8. 53 proteins having an upregulated expression simultaneously with a down-regulated ubiquitination and 116 proteins having down-regulated...