Data from: Optimizing techniques to capture and extract environmental DNA for detection and quantification of fish

Jessica J. Eichmiller, Loren M. Miller & Peter W. Sorensen
Few studies have examined capture and extraction methods for environmental DNA (eDNA) to identify techniques optimal for detection and quantification. In this study, precipitation, centrifugation and filtration eDNA capture methods and six commercially available DNA extraction kits were evaluated for their ability to detect and quantify common carp (Cyprinus carpio) mitochondrial DNA using quantitative PCR in a series of laboratory experiments. Filtration methods yielded the most carp eDNA, and a glass fibre (GF) filter performed...

Registration Year

  • 2015
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Resource Types

  • Dataset
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Affiliations

  • University of Minnesota
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  • Minnesota Department of Natural Resources
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