Additional file 1: Figure S1. Full-length blots/gels of ABCA1, MMP9, AQP4, CYP46, and beta-actin are presented. Figure S2. Full-length blots/gels of phospho-PI3K, PI3K, AKT, phospho-AKT, eNOS, phospho-eNOS and beta-actin are presented. Figure S3. Full-length blots/gels of ABCA1 and beta-actin are presented. Figure S4. Full-length blots/gels of MMP9, AQP4, CYP46 and β-actin are presented. Figure S5. Full-length blots/gels of phospho-PI3K, PI3K, AKT, phospho-AKT, eNOS, phospho-eNOS and β-actin are presented.

Additional file 1: Table S1. Treatment information of overall patients.

Abstract Background Pancreatic signet ring cell carcinoma (PSRCC) is a rare tumour subtype with poorly understood epidemiological characteristics and prognosis. We attempted to comprehensively characterise the epidemiology and survival outcomes of PSRCC. Methods Patients diagnosed with PSRCC between 2000 and 2018 were identified using Surveillance, Epidemiology and End Results Stat 8.3.9.2 software. Age-adjusted incidence and survival were calculated. Survival curves were plotted using the Kaplan–Meier method, and the differences between survival curves were compared using...

Additional file 3. Fig. S2. Depletion of Airn resulted in the decline of IMP2 at the protein level but not mRNA level.

Abstract Background Cardiac fibrosis is a leading cause of cardiac dysfunction in patients with diabetes. However, the underlying mechanisms of cardiac fibrosis remain unclear. This study aimed to investigate the role of the long non-coding RNA (LncRNA) Airn in the pathogenesis of cardiac fibrosis in diabetic cardiomyopathy (DCM) and its underlying mechanism. Methods Diabetes mellitus (DM) was induced in mice by streptozotocin injection. An intramyocardial adeno-associated virus (AAV) was used to manipulate Airn expression. The...

Additional file 3: Fig. S3. qRT-PCR analysis of FZD2, FZD3, FZD4 and FZD7 in NSCLC cells and HBE cells. All data were normalized to the expression level in HBE cells. *, P < 0.05, **, P < 0.01***, P < 0.001.

Additional file 1. A Body weight of CIA rats. B Arthritis index of CIA rats (* P-value < 0.05).

Abstract Backgrounds Rheumatoid arthritis (RA) is a chronic inflammatory and autoimmune disease. Current studies suggest that long noncoding RNAs (lncRNAs) may be key regulators in pathogenesis. Methods Analyzed lncRNAs and mRNAs using microarrays to find key differentially expressed lncRNAs in RA. GO and KEGG enrichment analysis together with coding non-coding co-expression (CNC) network was used for comprehensive analysis. Verify that their expression levels are consistent with the chip results by qRT-PCR. Results There are 268...

Additional file 1: Figure S1. Design the articles based on Haddon model and beliefs the parents needed.

Additional file 5: Table S2. The details of questionnaire.

Additional file 7: Table S4. Theeffectiveness of primary and secondary outcome within each group.

Abstract Background It has not yet been determined whether gastroscopy and colonoscopy screening help patients with gallbladder diseases. We aim to retrospectively investigate the relationship between gallbladder diseases and gastrointestinal polyps in order to provide a theoretical basis for the early screening of gastrointestinal polyps in patients with gallbladder disease. Methods This is a retrospective cross-sectional study involving 1662 patients who underwent gastroscopy, colonoscopy, and abdominal ultrasound as part of their health check-up from January...

Fasting glucose variability (FGV) extensively promotes the onset and development of diabetic complications. This study aimed to evaluate the FGV in type 2 diabetes mellitus (T2DM) patients administered basal insulin using a needle-free insulin injector (NFII). This was a prospective randomized multicenter open-label crossover study. We randomly assigned 48 T2DM patients to receive basal insulin by NFII or conventional insulin pen (CIP) for 7–14 days and were then crossed over after washout. We conducted continuous...

Additional file 1: Figure S1. Correlation between SLIT2 methylation density detected by BSP and SLIT2 methylation level detected by RT-qMSP.

Additional file 1: Figure S1. Correlation between SLIT2 methylation density detected by BSP and SLIT2 methylation level detected by RT-qMSP.

Additional file 1.

Primula odontocalyx (Franch.) Pax (1905) is a perennial herb of the genus Primula in Primulaceae with heterostyly and ornamental value. Here, the chloroplast genome of P. odontocalyx was sequenced, assembled, and annotated. The complete chloroplast genome was a closed-circular molecule of 151,738 bp in length, containing a large single-copy region (LSC) of 83,817 bp, a small single-copy region (SSC) of 17,529 bp, and two inverted repeat (IR) regions of 25,196 bp. A total of 115...

Additional file 2: Table S2. List of total circRNAs detected in the DRG of NC and CCI rats. RNA-seq was performed to profile circRNA expression in four paired DRG samples. A total of 13,172 circRNAs were detected in the L4–L5 DRG samples from the CCI and NC groups (4 mice per group).

Additional file 1. Supplementary description of cell lines. In this additional file, the characteristics and culture methods of bicalutamide sensitive cells and resistant cells, as well androgen-dependent and independent cells were detailly described.

Additional file 2: Fig. S1. JAK1 overexpression suppresses EC cell growth and migration.

Additional file 3: Fig. S2. JAK1 knockdown elevates the expression of HIF downstream genes in SPEC-2 cells.

Additional file 4: Fig. S3. JAK1 overexpression downregulates the expression of HIF downstream genes in Ishikawa cells.

Abstract Background Near-miss organizational learning is important for perspective and proactive risk management. Although nursing organizations are the largest component of the healthcare system and act as the final safety barrier, there is little research about the current status of near-miss organizational learning. Thus, we conducted this study to explore near-miss organizational learning in a Chinese nursing organization and offer suggestions for future improvement. Methods This was a mixed methods study with an explanatory sequence....

Abstract Background Pregnant women with pulmonary hypertension (PH) have higher mortality rates and poor foetal/neonatal outcomes. Tools to assess these risk factors are not well established. Methods Predictive and prognostic nomograms were constructed using data from a “Development” cohort of 420 pregnant patients with PH, recorded between January 2009 and December 2018. Logistic regression analysis established models to predict the probability of adverse maternal and foetal/neonatal events and overall survival by Cox analysis. An independent...

Additional file 1: Figure S1. The expression conditions between tumor and paired normal pancreas among 13 genes in pancreatic cancer patients. A-M Compared to the paired normal pancreas, tumor tissue of most pancreatic cancer patients in GSE15471 has lower CHRNB2 expression. (**: P < 0.01; ***: P < 0.001).