Additional file 5: Fig. S5. A–D. Western blot analysis of Nrf-2 or HO-1 expression after transfected with ML385 or siHO-1, GAPDH were used as the loading control. And bar graphs show the results of analysis (by band density analysis) of these proteins (n = 3). Data are presented as the means ± SD; *P < 0.05 vs. control, #P < 0.05 vs. Glu + siCtrl group, and n.s.: no significant difference

Experimental characterization

Supplementary Material 2

It is important to develop statistical techniques to analyze high-dimensional data in the presence of both complex dependence and possible heavy tails and outliers in real-world applications such as imaging data analyses. We propose a new robust high-dimensional regression with coefficient thresholding, in which an efficient nonconvex estimation procedure is proposed through a thresholding function and the robust Huber loss. The proposed regularization method accounts for complex dependence structures in predictors and is robust against...

Abstract Background To adapt to life on land, Asiatic toads (Bufo gargarizans) must remodel their bodies and refine their chemical defenses in water. The full scope of the mechanisms underlying these processes has yet to be revealed. Bufadienolides (BDs) are chemical defense substances secreted by toads when they are in danger, and they have high medicinal value in treating heart failure, cancer, and hepatitis. However, the artificial breeding of toads to increase BDs has been...

Abstract Background Monte Carlo simulation is considered as the most accurate method for dose calculation in radiotherapy. PRIMO is a Monte-Carlo program with a user-friendly graphical interface. Material and method A VitalBeam with 6MV and 6MV flattening filter free (FFF), equipped with the 120 Millennium multileaf collimator was simulated by PRIMO. We adjusted initial energy, energy full width at half maximum (FWHM), focal spot FWHM, and beam divergence to match the measurements. The water tank...

Additional file 1. The transport parameters configuration in PRIMO and gamma passing rates for PDD and OAR.

Additional file 1. SPIRIT 2013 Checklist.

Additional file 2. Standardized Process and Indicator Definition.

Additional file 1. The detailed primer information of markers used in initial genetic mapping and fine mapping of BhWAX, and primers of qRT-PCR used in the study.

Abstract Background Wax gourd [Benincasa hispida (Thunb) Cogn. (2n = 2x = 24)] is an economically important vegetable crop of genus Benincasa in the Cucurbitaceae family. Fruit is the main consumption organ of wax gourd. The mature fruit cuticular wax (MFCW) is an important trait in breeding programs, which is also of evolutionary significance in wax gourd. However, the genetic architecture of this valuable trait remains unrevealed. Results In this study, genetic analysis revealed that...

Additional file 1: Table S1. Intra- and inter-observer reliability of measurements.

Changes in the activated sludge performance in an anaerobic/aerobic biological treatment system for leachate was discussed under the condition of tetracycline (TC) exposure. The results show that a low concentration of TC did not have an obvious effect on the removal of chemical oxygen demand (COD) while a high concentration of TC had a certain promoting effect. Under the stimulation of TC, the particle size distribution of anaerobic/aerobic sludge tended to be more uniform, the...

Abstract Background Intrahepatic cholangiocarcinoma (ICC), originating from the bile ducts, is the second most common primary liver malignancy, and its incidence has recently increased. H2A.Z, a highly conserved H2A variant, is emerging as a key regulatory molecule in cancer. However, its underlying mechanism of action in ICC cells remains unclear. Methods Here, we examined the expression of H2A.Z and SFRP1 in normal intrahepatic cholangiocytes, ICC cell lines, ICC tissue microarrays, and fresh specimens. The correlations...

Additional file 1: Table S1. Raw data for IHC staining.

Additional file 4: Table S4. Gene list of positively and differentially co-expressed genes of ZNF71.

Additional file 5: Table S5. Pearson correlation analysis between ZNF71 and its putative target genes which relates to tight junction.

Supplementary Material for The association between preoperative blood calcium and postoperative blood loss in patients undergoing heart valve replacement surgery by Mingke Li, Yawei Dai, Shuai Hu, Yansong Li, Dasheng Lu, Rui Zheng, and Kai Wang in Perfusion.

Additional file 5: Figure S2. Subcellular localization motifs of CBL proteins at the N-terminus.

Abstract Background Calcineurin B-like proteins (CBLs) are ubiquitous Ca2+ sensors that mediate plant responses to various stress and developmental processes by interacting with CBL-interacting protein kinases (CIPKs). CBLs and CIPKs play essential roles in acclimatization of crop plants. However, evolution of these two gene families in the genus Medicago is poorly understood. Results A total of 68 CBL and 135 CIPK genes have been identified in five genomes from Medicago. Among these genomes, the gene...

Abstract Background Fetal skeletal dysplasia (SD) is a common congenital disability comprising a complex group of skeletal disorders with substantial clinical and genetic heterogeneity. Many of these defects are detected prenatally using ultrasound (US). However, the diagnostic accuracy of the US is limited. Methods We recruited 55 unrelated fetuses with US-detected skeletal anomalies and performed sequential tests using copy number variation sequencing, targeted skeletal gene panel sequencing, or whole exome sequencing. The detected variants were...

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Performance measurement of the electrical devices

Additional file 1: Figure S1. Pie chart shows the number of differentially expressed lncRNAs in each category.

Additional file 1: Fig. S1. Analysis of the expression data from GEO database. The difference of the expression of H2A.Z and SFRP1 between ICC tissue and normal bile duct was visualized by box plot. (A) GSE32879. (B) GSE76297. **p<0.01, ***p<0.001.