Additional file 1: Supplemental Table 1. The ICC values of quantitative parameters between three radiologists.

Strawberry has high nutritional and economic value and is an important horticultural crop widely cultivated worldwide. Strawberry anthracnose is the most harmful disease in strawberry production, which causes huge production losses every year. Mining disease resistance genes and breeding high-quality strawberry varieties are the fundamental way to solve the problem of strawberry anthracnose. In this study, transcriptome analysis and disease resistance-related genes mining were targeted on strawberry anthracnose. The main results are as follows: (1)...

Additional file 5: Fig. S5. Validation of the association between risk score and immune cell infiltration. A Scatter plot showed the positive correlation between the risk score and ImmuneScore (Spearman’s rank correlation coefficient) in the GSE16011 GBM cohort. B Scatter plot showed the positive correlation between the risk score and StromalScore (Spearman’s rank correlation coefficient) in the GSE16011 GBM cohort. C Scatter plot showed the positive correlation between the risk score and ESTAMEScore (Spearman’s rank...

Additional file 9: Table S4. The 21 prognositc DE-MRGs based on the TCGA GBM cohort.

Additional file 1. Supplementary Tables and Figures.

Additional file 10: Table S5. GO enrichment analysis by using DAVID.

Additional file 2: Fig. S2. The predictivity of 12 DE-MRGs model for other cancers. A The univariate cox analysis showed that the risk score, based on our 12 DE-MRGs, significantly associated with other 12 types of TCGA cancers. B Kaplan–Meier curves for OS in the other 12 types of TCGA cancers. The patients were divided in to high- and low-risk groups based on the median value of risk score.

Additional file 1. Animal protocol.

Additional file 1: Table S1. Association of selected risk factors with colorectal neoplasms stratified by sex.

Abstract Background Screening recommendations for colorectal cancer (CRC) are mainly based on family history rather than lifestyle risk factors. We aimed to assess and compare risk factors for colorectal neoplasm (CRN) and evaluate trends in neoplasm detection rates during the three rounds of screening from 2012 to 2020 in Tianjin, China. Methods This study was based on 89,535 first-recorded colonoscopies in Tianjin CRC screening program, 2012–2020. Of these, 45,380 individuals with complete family history and...

Additional file 4. miRNA sequences.

Additional file 6. Identification of hUCMSC, primary astrocyte and hUCMSC-derived exosomes. A hUCMSCs were identified by the stem cell makers (CD73, CD90 and CD105) and nonstem cell markers (CD45, CD34 and HLA-DR) expression using flow cytometry. B Primary astrocytes were characterized by immunostaining of GFAP, S100β, Vimentin and morphology in bright filed. C The morphology of exosomes detected by transmission electron microscopy. Scale bar = 200 nm. D Diameters and concentrations of exosomes analyzed by...

Additional file 8. The other 4 abundant miRNAs mimics’ effects on mRNA expression of neurotoxic astrocyte markers. Quantitative analysis of mRNA expression of C3 and Lcn2 in astrocytes, neurotoxic astrocytes and neurotoxic astrocytes treated with 50 nM mimics of miR-21-5p(a), miR-22-3p(b), miR-24-3p(c), miR-26a-5p(d) by RT-QPCR. Data above are represented as mean ± SD. *, p < 0.05; **, p < 0.01; #, p>0.05. C3 complement c3, Lcn2 lipocalin-2.

Additional file 3. Factors of the combined model predicting OS in patients with local residual tumors after IMRT in the training cohort

Abstract Background To investigate the potential value of the pretreatment MRI-based radiomic model in predicting the overall survival (OS) of nasopharyngeal carcinoma (NPC) patients with local residual tumors after intensity-modulated radiotherapy (IMRT). Methods A total of 218 consecutive nonmetastatic NPC patients with local residual tumors after IMRT [training cohort (n = 173) and validation cohort (n = 45)] were retrospectively included in this study. Clinical and MRI data were obtained. Univariate Cox regression and the...

This study aimed to investigate hypoalbuminemia-related factors in patients with stroke in the neurocritical care unit (NCU) and to establish a nomogram model for hypoalbuminemia prediction. Data from 902 patients hospitalized for stroke in the NCU who had normal albumin levels on admission from March 2018 to December 2020 were analyzed. Logistic regression was used to identify the risk factors associated with hypoalbuminemia. A novel nomogram prediction model for hypoalbuminemia was constructed, and it was...

Additional file 2: Figure S1. Depletion of LARRPM promoted proliferation, repressed apoptosis and promoted migration and invasion of LUAD cells. A LARRPM expression in HCC827 cells with LARRPM stable depletion or control was detected by qRT-PCR. B Cell proliferation of HCC827 cells with LARRPM stable depletion or control was detected using CCK-8 assays. C Cell proliferation of HCC827 cells with LARRPM stable overexpression or control was detected using EdU incorporation assays. Scale bar: 100 µm....

Additional file 6: Fig. S5. Anti-CSF1 antibody abolished the effects of LARRPM on M2 macrophage infiltration. A THP-1 cells were subjected to transwell migration assays towards A549 cells with LARRPM overexpression or A549 control cells treated with anti-CSF1. Scale bar: 100 µm. B THP-1 cells were subjected to transwell migration assays towards HCC827 cells with LARRPM depletion or HCC827 control treated with anti-CSF1. Scale bar: 100 µm. C M1 and M2 polarization markers expression in...

Abstract Background Long non-coding RNAs (lncRNAs) are critical regulators in lung adenocarcinoma (LUAD). M2-type tumor-associated macrophages (TAMs) also play oncogenic roles in LUAD. However, the involvement of lncRNAs in TAM activation is still largely unknown. Methods The expressions of LARRPM, LINC00240 and CSF1 were determined by RT-qPCR. The regulation of LINC00240 and CSF1 by LARRPM was investigated by RNA–protein pull-down, RNA immunoprecipitation, chromatin immunoprecipitation and bisulfite DNA sequencing. In vitro and in vivo gain- and...

Abstract Background Long non-coding RNAs (lncRNAs) are critical regulators in lung adenocarcinoma (LUAD). M2-type tumor-associated macrophages (TAMs) also play oncogenic roles in LUAD. However, the involvement of lncRNAs in TAM activation is still largely unknown. Methods The expressions of LARRPM, LINC00240 and CSF1 were determined by RT-qPCR. The regulation of LINC00240 and CSF1 by LARRPM was investigated by RNA–protein pull-down, RNA immunoprecipitation, chromatin immunoprecipitation and bisulfite DNA sequencing. In vitro and in vivo gain- and...

Additional file 6: Figure S6. HDAC2 inhibition promoted the expression of Serpine1. A SDS-PAGE identification of the proteins pulled-down by DNA fragment of Serpine1 promoter. The gel was stained with Coomassie blue. The indicated differential bands were excised with a scalpel and identified by LC-MS. B Total ion chromatogram of proteins. C–E Serpine1 expression analysed using RT-qPCR and western blot in trichostatin A (TSA)-treated MLE-12 cells. Data show mean ± SEM; Data was analysed using...

Additional file 6: Figure S6. HDAC2 inhibition promoted the expression of Serpine1. A SDS-PAGE identification of the proteins pulled-down by DNA fragment of Serpine1 promoter. The gel was stained with Coomassie blue. The indicated differential bands were excised with a scalpel and identified by LC-MS. B Total ion chromatogram of proteins. C–E Serpine1 expression analysed using RT-qPCR and western blot in trichostatin A (TSA)-treated MLE-12 cells. Data show mean ± SEM; Data was analysed using...

Additional file 15: Figure S6. circPVT1 promotes the migration and invasion of NPC cells by regulating cell adhesion andcytoskeleton remodeling. The 231 differentially expressed proteins regulated by c-Myc from LC-MS/MS data using the Ingenuity Pathway Analysis (IPA) software.

Additional file 10: Table S9. The number of different types of hydrogenase and sulfide quinone oxidoreductase (sqr) in microbial genomes.

Additional file 10: Table S9. The number of different types of hydrogenase and sulfide quinone oxidoreductase (sqr) in microbial genomes.