Objectives: To determine the frequency and correlates of apathy in amyotrophic lateral sclerosis (ALS) and its influence on the prognosis of the disease. Methods: Three databases were searched: MEDLINE, PubMed, and Google Scholar. Quantitative synthesis of the frequency of apathy in ALS was conducted using random effects in Stata (College Station, TX). Meta-regression and subgroup analyses were conducted to investigate the association between frequency of apathy in ALS and different covariates. Results: Fifty-two studies (51...

Atg11 is an adaptor protein required for the induction of selective autophagy via receptor binding. However, our understanding of the molecular mechanisms by which it regulates selective autophagy remains incomplete. Here, we show that Atg11 is phosphorylated by Atg1. Rapamycin treatment or starvation conditions induced slower electrophoretic mobility of Atg11 in an Atg1 kinase activity-dependent manner. Through in vitro kinase assays combined with mutagenesis, we determined that Atg1 phosphorylates S949, S1057, and S1064 residues in...

Atg11 is an adaptor protein required for the induction of selective autophagy via receptor binding. However, our understanding of the molecular mechanisms by which it regulates selective autophagy remains incomplete. Here, we show that Atg11 is phosphorylated by Atg1. Rapamycin treatment or starvation conditions induced slower electrophoretic mobility of Atg11 in an Atg1 kinase activity-dependent manner. Through in vitro kinase assays combined with mutagenesis, we determined that Atg1 phosphorylates S949, S1057, and S1064 residues in...

The antimicrobial activity and preservative effect of Ceylon spinach (Basella alba L.) seed protein extract on sturgeon fillets stored at 4°C were investigated in this study. The results showed that Ceylon spinach seed protein extract (CSSPE) had inhibitory effect on 33 microorganisms, including 1 fungus and 32 bacteria (25 gram-negative bacteria and 7 gram-positive bacteria). In addition, CSSPE inhibited bacterial growth (total viable counts, Pseudomonas, Aeromonas and psychrotrophs), suppressed ATP degradation and retained good-quality characteristic...

The DEAD-box family has been shown to play an important role in a variety of abiotic stresses, but little is known in studies of mangrove plants. Here, the effects of cold stress on various physiological changes and the role of the DEAD-box RNA helicase family in response to cold stress were determined. First, we identified 73 DEAD-box RNA helicase family members in L. littorea. Second, the evolutionary relationships between the DEAD family in L. littorea...

Additional file 1. Supplementary Material for a Successful Hybrid Deep Learning Model aiming at Promoter Identification.

The DEAD-box family has been shown to play an important role in a variety of abiotic stresses, but little is known in studies of mangrove plants. Here, the effects of cold stress on various physiological changes and the role of the DEAD-box RNA helicase family in response to cold stress were determined. First, we identified 73 DEAD-box RNA helicase family members in L. littorea. Second, the evolutionary relationships between the DEAD family in L. littorea...

Resembling acute promyelocytic leukemia (APL) is a unique subtype of APL who sharing clinical, morphological, and immunophenotypic features with typical APL, but lacking evidence of PML-RARA fusion gene and usually insensitive to arsenic trioxide (ATO) and all-trans retinoic acid (ATRA). For years, RARA, RARB and RARG rearrangement were found in resembling APL continually. The confirmed partner genes of RARG rearrangement included CPSF6, NUP98, NPM1, PML, and HNRNPC. These patients were a group of resembling APL...

Additional file 11: Figure S2. circPVT1 promotes the migration and invasion of NPC in vitroand metastasis of NPCin vivo. A-B. The expression of circPVT1 were measured in CNE2 and 5-8F cells after transfection with the circPVT1 overexpression vector or circPVT1 siRNA. PVT1 expression was not affected in CNE2 and 5-8F cells after overexpresion or knockdown of circPVT. All experiments were repeated at least three times. Data were represented as mean ± SD. ***, p <...

Additional file 11: Figure S2. circPVT1 promotes the migration and invasion of NPC in vitroand metastasis of NPCin vivo. A-B. The expression of circPVT1 were measured in CNE2 and 5-8F cells after transfection with the circPVT1 overexpression vector or circPVT1 siRNA. PVT1 expression was not affected in CNE2 and 5-8F cells after overexpresion or knockdown of circPVT. All experiments were repeated at least three times. Data were represented as mean ± SD. ***, p <...

Additional file 12: Figure S3. circPVT1 promotes the migration and invasion of NPC cells through binding to β-TrCP. A. The secondary ion flow diagram of β-TrCP identified by mass spectrometry according to the peptide sequence (DFITALPAR) of β -TrCP.B. Schematic diagram of binding sites betweent circPVT1 and β-TrCP using the catRAPID website.The 230-280 nt of circPVT1 was predicted to bind to β-TrCP protein with high interaction score.C. The secondary structure of circPVT1 changed when 230-280...

Additional file 13: Figure S4. The WD40 repeat domain of β-TrCP interacts with circPVT1. A. The functional domain of the of β-TrCP proteins are illustrated (top), including the F-box domain and the WD40 domain. The expressing plasmids for full-length β-TrCP or truncated fragments (F-box and WD40) were constructed and confirmed using anti-Flag antibody. B. Wound healing assay showed that overexpression of β-TrCP inhibited NPC cells migration. Data were represented as mean ± SD. ***, p...

Additional file 14: Figure S5. c-Myc is ubiquitinated substrate of β-TrCP. A. The expression of β-TrCP was examined in CNE2 and 5-8F cells after overexpression or knockdown of circPVT1 using western blotting.B. The β-TrCP binding proteins were identified in CNE2 cells by immunoprecipitation followed by LC-MS/MS.C. The interaction of c-Myc and β-TrCP was predicted by Molecular Docking ( http://hdock.phys.hust.edu.cn/ ). The blue part is the WD40 repeat domain of β-TrCP.D. The expression of c-Myc was...

Additional file 1: Supplemental Table 1. Clinicopathological data of 60 NPC and 26 NPE tissues measured by qRT-PCR.

Additional file 2: Figure S2. CISD2 expression endows the cellular resistance to ferroptosis inducer. (A–D) Analysis of the CISD2 expression by western blot in HT-1080 (A, C) or HL60 (B, D) cells, GAPDH was used as loading control; (E, F) Cell survival analysis in CISD2-modified HT-1080 cells after the treatment of Erastin (0–10 µM); (G, H) Cell survival analysis in CISD2-modified HL60 cells after the treatment of Erastin (0–15 µM); Cell survival in control cells...

Additional file 2: Figure S2. CISD2 expression endows the cellular resistance to ferroptosis inducer. (A–D) Analysis of the CISD2 expression by western blot in HT-1080 (A, C) or HL60 (B, D) cells, GAPDH was used as loading control; (E, F) Cell survival analysis in CISD2-modified HT-1080 cells after the treatment of Erastin (0–10 µM); (G, H) Cell survival analysis in CISD2-modified HL60 cells after the treatment of Erastin (0–15 µM); Cell survival in control cells...

Abstract Background Glioblastoma (GBM) is the most common primary malignant brain tumor that leads to lethality. Several studies have demonstrated that mitochondria play an important role in GBM and that mitochondria-related genes (MRGs) are potential therapeutic targets. However, the role of MRGs in GBM remains unclear. Methods Differential expression and univariate Cox regression analyses were combined to screen for prognostic differentially-expressed (DE)-MRGs in GBM. Based on LASSO Cox analysis, 12 DE-MRGs were selected to construct...

Additional file 7: Table S2. The primer sequences used in the study.

Additional file 1: Fig. S1. Validation of DE-MRGs related prognostic risk model for GBM. A, E Time dependent ROC curves for 12 DE-MRGS prognostic model in the GSE147352 and GSE16011 GBM cohort. B, F The distribution of risk scores, survival time, and status of GBM patients in the GSE147352 and GSE16011 GBM cohort. C, GThe heatmap of the 12 model DE-MRGs in the GSE147352 and GSE16011 GBM cohort. D, H Kaplan–Meier curves for OS in...

Additional file 3: Fig. S3. The time dependent ROC curves for other three prognostic models. A Time dependent ROC curves for immune-related gene signature in the TCGA GBM cohort. B Time dependent ROC curves for pyroptosis-related gene signature in the TCGA GBM cohort. C Time dependent ROC curves for autophagy-related gene signature in the TCGA GBM cohort.

Additional file 4: Fig. S4. Validation of independence of our risk score as a prognostic factor. A The forest plot showed the univariate cox analysis using risk score, age, radiotherapy, TMZ chemotherapy, and gender as variates in the GSE16011 GBM cohort. B The forest plot showed the multivariate cox analysis using risk score, age, radiotherapy, and TMZ chemotherapy as variates in the GSE16011 GBM cohort. C Stratified OS analysis in GSE16011 GBM patients with different...

Additional file 3: Figure S3. The effect of Serpine1 overexpression in lung epithelial cells. A Serpine1 expression analysed using RT-qPCR in Serpine1-overexpressing MLE-12 cells. B–D Serpine1 expression analysed using RT-qPCR, western blot and Immunofluorescence in Serpine1-overexpressing A549 cells. E, F Apoptosis rate analysed using flow cytometry in Serpine1-overexpressing A549 cells. G p16 expressions analysed using western blot in Serpine1-overexpressing BEAS-2B cells. Data show mean ± SEM; Data was analysed using unpaired t-tests.

Additional file 4: Figure S4. Enrichment of differentially expressed genes in Serpine1-overexpressing MLE-12 cells. A, B GO and KEGG enrichment of the upregulated genes. C, D GO and KEGG enrichment of the downregulated genes. Q-value ≤ 0.05 and fold-change ≥ 2 were used as the thresholds for screening the differentially expressed genes.

Additional file 2: Figure S2. Genes expression levels in lung tissues of neonatal mice (A) The mRNA expression of differentially expressed genes analysed using RT-qPCR in lung tissues of neonatal mice. B, C Nr4a1 expression analysed using western blot in lung tissues of neonatal mice. D Serpine1 expression in lung tissues of neonatal mice detected using RT-qPCR on P1, P7, and P21. E, F Serpine1 expression analysed using RT-qPCR and western blot in siRNA-treated neonatal...

Additional file 3: Figure S3. The effect of Serpine1 overexpression in lung epithelial cells. A Serpine1 expression analysed using RT-qPCR in Serpine1-overexpressing MLE-12 cells. B–D Serpine1 expression analysed using RT-qPCR, western blot and Immunofluorescence in Serpine1-overexpressing A549 cells. E, F Apoptosis rate analysed using flow cytometry in Serpine1-overexpressing A549 cells. G p16 expressions analysed using western blot in Serpine1-overexpressing BEAS-2B cells. Data show mean ± SEM; Data was analysed using unpaired t-tests.