Additional file 8 Table S3. KEGG enrichment analysis of the MAPK8IP2 gene and its partner genes.

Abstract Background MAPK8IP2 is one of the JNK-interacting proteins (JIPs) family members, and is involved in the regulation of the JNK and P38 MAPK signaling pathways. MAPK8IP2 has been reported to be closely associated with several cancers. However, the biological function of MAPK8IP2 in prostate cancer (PCa) remains unclear. Methods MAPK8IP2 expression in PCa and subgroups of PCa was analyzed by public databases. The prognostic role of MAPK8IP2 in prostate cancer was analyzed using the...

Quantum key distribution (QKD) is an effective solution to ensure the secure transmission of information. However, for the large-scale application of QKD, the interoperability and flexibility of transmitter and receiver is an urgent issue to be solved. Here, for the first time, we experimentally verify the feasibility of one continuous-variable (CV) QKD system to achieve multiple protocols and rates. The flexibility of the system comes from the modulator realizing multiple protocols and a broadband coherent...

Quantum key distribution (QKD) is an effective solution to ensure the secure transmission of information. However, for the large-scale application of QKD, the interoperability and flexibility of transmitter and receiver is an urgent issue to be solved. Here, for the first time, we experimentally verify the feasibility of one continuous-variable (CV) QKD system to achieve multiple protocols and rates. The flexibility of the system comes from the modulator realizing multiple protocols and a broadband coherent...

Discrimination, the ability to distinguish sensory stimuli and respond accordingly, is a critical factor underscoring optimal foraging decisions. Nevertheless, little is known about how mammals discriminate between apparently similar foods of different quality. Here, we compared the foraging behavior of Chinese white-bellied rats, Niviventer confucianus, and Edwards’s long-tailed giant rats, Leopoldamys edwardsi, under natural conditions in the field and in a captive enclosure without predation/competition. We examined the behavioral processes involved in discriminating between sound...

This study aims to investigate the potential mechanisms and identify core biomarkers of Hepatocellular carcinoma (HCC). The profile GSE113850 was downloaded to analyze the differentially expressed genes. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein–protein interaction network analysis were used to reveal the main signal pathways of the differentially expressed genes (DEGs) and hub genes. The correlation between core gene expression and pathological stages, the disease-free survival analysis, the overall survival...

Co-occurrence of hypervirulence and KPC-2 carbapenem resistant phenotypes in a highly-transmissible ST11 clone ofKlebsiella pneumoniae has elicited deep concerns from public health stand point. To address this puzzle, we conducted a large-scale epidemiological, clinical and genomic study of K. pneumonia ST11 clones with both hypervirulence and carbapenem resistance in two tertiary hospitals in Zhejiang province. Most of the patients (15/23) were diagnosed with exclusively carbapenem-resistant K. pneumoniae (CRKP) infections. Ten death cases were reported, some...

Biodiversity experiments have shown that species loss reduces ecosystem functioning in grassland. To test whether this result can be extrapolated to forests, the main contributors to terrestrial primary productivity, requires large-scale experiments. We manipulated tree species richness by planting more than 150,000 trees in plots with 1 to 16 species. Simulating multiple extinction scenarios, we found that richness strongly increased stand-level productivity. After 8 years, 16-species mixtures had accumulated over twice the amount of carbon...

With the decreasing cost and availability of many newly developed bioinformatics pipelines, next-generation sequencing (NGS) has revolutionized plant systematics in recent years. Genome skimming has been widely used to obtain high-copy fractions of the genomes, including plastomes, mitochondrial DNA (mtDNA), and nuclear ribosomal DNA (nrDNA). In this study, through simulations, we evaluated the optimal (minimum) sequencing depth and performance for recovering single-copy nuclear genes (SCNs) from genome skimming data, by subsampling genome resequencing data and...

Introduction:Venovenous extracorporeal membrane oxygenation (VV ECMO) is now considered a reasonable option to salvage acute respiratory distress syndrome (ARDS). However, we lack a rodent model for experimental studies. This study was undertaken to establish an animal model of VV ECMO in ARDS rats.Methods:A total of 18 Sprague-Dawley (SD) rats (350 ± 50 g) were used in this study. Using a rat model of oleic acid (OA)-induced ARDS, VV ECMO was established through cavoatrial cannulation of...

Introduction:Venovenous extracorporeal membrane oxygenation (VV ECMO) is now considered a reasonable option to salvage acute respiratory distress syndrome (ARDS). However, we lack a rodent model for experimental studies. This study was undertaken to establish an animal model of VV ECMO in ARDS rats.Methods:A total of 18 Sprague-Dawley (SD) rats (350 ± 50 g) were used in this study. Using a rat model of oleic acid (OA)-induced ARDS, VV ECMO was established through cavoatrial cannulation of...

Single nucleotide polymorphisms (SNPs) include the transition and transversion of a single base. In this study, we found two cSNPs (rs110757796 and rs110652478) in the FABP4 (fatty acid-binding protein 4) gene and tested their population genetic parameters in five Chinese cattle breeds. FABP4 is a key marker molecule for lipid production. It plays a crucial role in the growth and development of animals. Thus, we also analyze the association between the two cSNPs of FABP4...

To investigate the differential expression of microRNA-150-5p (miR-150-5p) and early growth response 1 (EGR1) in myocardial fibrosis (MF) cells, and determine the effect between miR-150-5p and EGR1 on MF. Human MF cells were generated via Trypanosoma cruzi (T. cruzi) infection, a mouse model of MF was generated via angiotensin II. The expression levels of miR-150-5p and EGR1 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot assay. The correlation between miR-150-5p and...

To investigate the differential expression of microRNA-150-5p (miR-150-5p) and early growth response 1 (EGR1) in myocardial fibrosis (MF) cells, and determine the effect between miR-150-5p and EGR1 on MF. Human MF cells were generated via Trypanosoma cruzi (T. cruzi) infection, a mouse model of MF was generated via angiotensin II. The expression levels of miR-150-5p and EGR1 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot assay. The correlation between miR-150-5p and...

The precise age of slab rollback of the Paleo-Pacific Plate during Early Cretaceous has been contentious. In this study, we have conducted LA-ICP-MS zircon U-Pb dating, major and trace elements, and Sr-Nd isotopes analyses on the late Early Cretaceous diabase porphyries, andesitic basalts and basanites in the Yanbian area (NE China). Zircon U-Pb dating of basanites, andesitic basalts, and diabase porphyries yielded weighted average ages of 104 ± 1.9 Ma (MSWD = 0.55, n =...

Ramie bast is a kind of multipurpose biological material. Selective bio-degradation of ramie bast compounds is an important step to partial utilization of complex polysaccharides. Bacillus subtilis is a kind of dominant species with its ability to degrade ramie bast. In order to understand how polysaccharides were catalyzed and utilized in the metabolism of the bacteria, the proteome of B. subtilis strain 168 was analyzed in the presence of ramie bast as sole carbon source,...

Four new cembrane diterpenes, 1,13-di-epi-13-acetoxy launine P (1), 13-oxo-thunbergol (2), isocrassumol B (3) and 7α, 8α-sarcophine (4), together with two known launine P (5) and sarcophytonin B (6), were isolated from the extract of the South China Sea soft coral Sarcophyton trocheliophorum. The structures were elucidated by spectroscopic analysis, ECD analysis and comparison with literature data. All compounds were tested for their antimicrobial activity and antiviral activity. Compounds 1, 2 and 5 were found to...

Payments for Ecosystem Services (PES) programs have been implemented globally to protect ecosystems while securing the well-being of affected people. Reasonable payment standards are key to successful PES programs. Although some approaches are available for determining payment standards, few studies have applied them for grassland conservation with location indicators and socioeconomic contexts properly considered. Using China’s first pilot Grassland Ecological Compensation Policy (GECP) as an example, we analyzed the effects of payment levels, other natural...

Additional file 1: Fig. S1. Length distribution of the unigenes. Fig. S2. Expression level distribution of the unigenes. Fig. S3. Correlations between samples. Fig. S4. Clustering of samples. Fig. S5. Venn plot of the expressed genes in each tissues. The overlapping regions represent common expressed genes in the same tissue of different individuals. Fig. S6. Number of DEGs in each COG category. Fig. S7. Number of DEGs in the corresponding GO terms. Fig. S8. Significantly...

Primary ovarian insufficiency (POI), also known as premature ovarian failure, is an ovarian defect in humans characterized by the premature depletion of ovarian follicles before the age of 40. However, the mechanisms underlying POI remain largely unknown. Here, we show that knockout of Epg5 (ectopic P-granules autophagy protein 5 homolog (C. elegans)) results in subfertility in female mice, which exhibit a POI-like phenotype. Single-cell RNA sequencing analysis revealed that the knockout of Epg5 affected the...

Lung cancer has been recognized as the most common malignant neoplasm of the respiratory system with extremely high morbidity, among which non-small cell lung cancer (NSCLC) accounts for the majority. Many published literatures have revealed the roles of GSG2 in the progression of ovarian cancer, bladder cancer and breast cancer. However, there were no reports on the relationship between GSG2 and NSCLC. Herein, GSG2 was identified as a potential tumor promoter in NSCLC development, whose...

Muscle development is a complex process that was regulated by many factors, among which non-coding RNAs (ncRNAs) play a vital role in regulating multiple life activities of muscle cells. Circular RNA (circRNA), a type of non-coding RNA with closed-loop structure, has been reported to affect multiple life processes. However, the roles of circRNAs on muscle development have not been fully elucidated. The present study aimed to determine whether and how circRIMKLB affects muscle development. Our...

Buffalo holds an excellent potential for beef production, and circRNA plays an important role in regulating myogenesis. However, the regulatory mechanism of circRNAs during buffalo skeletal muscle development has not been fully explored. In this study, circRNA expression profiles during the proliferation and differentiation stages of buffalo myoblasts were analysed by RNA-seq. Here, a total of 3,142 circRNAs candidates were identified, and 110 of them were found to be differentially expressed in the proliferation and...

Supplementary Materials for CCT and experimental results

Abstract Chemotherapy is the main treatment option for advanced osteosarcoma, which is the most common type of primary bone malignancy. However, patients develop resistance rapidly and many succumb to the disease. Niclosamide, an anthelmintic drug, has been recently identified to display potent and selective anti-cancer activity. In this work, we show that niclosamide at sub-micromolar concentrations inhibits proliferation and migration, and induces apoptosis in both parental and chemo-resistant osteosarcoma cells, with much less toxicity in...