24 Works

Multiple abiotic and biotic drivers of long-term wood decomposition within and among species in semiarid inland dunes: a dual role for stem diameter

Enkhmaa Erdenebileg, Wang Congwen, Xuehua Ye, Qingguo Cui, Juan Du, Zhenying Huang, Guofang Liu & Johannes H. C. Cornelissen
This is wood decomposition data conducted in an semiarid inland dune including mass loss, initial wood traits and k values of combinations between five diameter classes and four shrub species under different treatments including UV and litter position. We found that after 34 months of in situ incubation, the mass loss of buried woody litters was three times faster than those of suspended and surface woody litters (53.5 ± 2.7 %, 17.0 ± 1.0 %...

Kinetics of SARS-CoV-2 specific IgM and IgG responses in COVID-19 patients

Baoqing Sun, Ying Feng, Xiaoneng Mo, Peiyan Zheng, Qian Wang, Pingchao Li, Ping Peng, Xiaoqing Liu, Zhilong Chen, Huimin Huang, Fan Zhang, Wenting Luo, Xuefeng Niu, Peiyu Hu, Longyu Wang, Hui Peng, Zhifeng Huang, Liqiang Feng, Feng Li, Fuchun Zhang, Fang Li, Nanshan Zhong & Ling Chen
The emerging COVID-19 caused by SARS-CoV-2 infection poses severe challenges to global public health. Serum antibody testing is becoming one of the critical methods for the diagnosis of COVID-19 patients. We investigated IgM and IgG responses against SARS-CoV-2 nucleocapsid (N) and spike (S) protein after symptom onset in the intensive care unit (ICU) and non-ICU patients. 130 blood samples from 38 COVID-19 patients were collected. The levels of IgM and IgG specific to N and...

Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells

Hua-Long Xiong, Yang-Tao Wu, Jia-Li Cao, Ren Yang, Ying-Xia Liu, Jian Ma, Xiao-Yang Qiao, Xiang-Yang Yao, Bao-Hui Zhang, Ya-Li Zhang, Wang-Heng Hou, Yang Shi, Jing-Jing Xu, Liang Zhang, Shao-Juan Wang, Bao-Rong Fu, Ting Yang, Sheng-Xiang Ge, Jun Zhang, Quan Yuan, Bao-Ying Huang, Zhi-Yong Li, Tian-Ying Zhang & Ning-Shao Xia
The global pandemic of coronavirus disease 2019 (COVID-19) is a disaster for human society. A convenient and reliable neutralization assay is very important for the development of vaccines and novel drugs. In this study, a G protein-deficient vesicular stomatitis virus (VSVdG) bearing a truncated spike protein (S with C-terminal 18 amino acid truncation) was compared to that bearing the full-length spike protein of SARS-CoV-2 and showed much higher efficiency. A neutralization assay was established based...

A dataset of plant and microbial community structure after long-term grazing and mowing in a semiarid steppe

Wenhuai Li, Jasna Hodzic, Jishuai Su, Shuxia Zheng & Yongfei Bai
Grazing and mowing are two dominant management regimes used in grasslands. Although many studies have focused on the effects of grazing intensity on plant community structure, far fewer test how grazing impacts the soil microbial community. Furthermore, the effects of long-term grazing and mowing on plant and microbial community structure are poorly understood. To elucidate how these management regimes affect plant and microbial communities, we collected data from 280 quadrats in a semiarid steppe after...

Longitudinal analysis of the antibody repertoire of a Zika virus-infected patient revealed dynamic changes in antibody response

Xuefeng Niu, Qihong Yan, Zhipeng Yao, Fan Zhang, Linbing Qu, Chunlin Wang, Chengrui Wang, Hui Lei, Chaoming Chen, Renshan Liang, Jia Luo, Qian Wang, Lingzhai Zhao, Yudi Zhang, Kun Luo, Longyu Wang, Hongkai Wu, Tingting Liu, Pingchao Li, Zhiqiang Zheng, Yee Joo Tan, Liqiang Feng, Zhenhai Zhang, Jian Han, Fuchun Zhang … & Ling Chen
The Zika virus (ZIKV) is a mosquito-borne flavivirus that causes neonatal abnormalities and other disorders. Antibodies to the ZIKV envelope (E) protein can block infection. In this study, next-generation sequencing (NGS) of immunoglobulin heavy chain (IgH) mRNA transcripts was combined with single-cell PCR cloning of E-binding monoclonal antibodies for analysing antibody response in a patient from the early stages of infection to more than one year after the clearance of the virus. The patient's IgH...

Profiling serum cytokines in COVID-19 patients reveals IL-6 and IL-10 are disease severity predictors

Huan Han, Qingfeng Ma, Cong Li, Rui Liu, Li Zhao, Wei Wang, Pingan Zhang, Xinghui Liu, Guosheng Gao, Fang Liu, Yingan Jiang, Xiaoming Cheng, Chengliang Zhu & Yuchen Xia
Since the outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, China, it has rapidly spread across many other countries. While the majority of patients were considered mild, critically ill patients involving respiratory failure and multiple organ dysfunction syndrome are not uncommon, which could result death. We hypothesized that cytokine storm is associated with severe outcome. We enrolled 102 COVID-19 patients who were admitted to Renmin Hospital (Wuhan, China). All patients were classified into moderate, severe...

Long non-coding RNA XIST promotes the progression of esophageal squamous cell carcinoma through sponging miR-129-5p and upregulating CCND1 expression

Haoran Wang, Haomiao Li, Yongkui Yu, Qingfeng Jiang, Ruixiang Zhang, Haibo Sun, Wenqun Xing & Yin Li
Long non-coding RNA (lncRNA) X inactive specific transcript (XIST) has been identified as an oncogenic lncRNA in a series of human cancers, including esophageal squamous cell carcinoma (ESCC). In this study, we aimed to further explore the underlying mechanism of XIST on ESCC progression. qRT-PCR assay was used to determine the levels of XIST and miR-129-5p. Western blot analysis was performed to assess cyclin D1 (CCND1) expression. Bioinformatic analysis was performed using starBase v2.0 software....

High anal swab viral load predisposes adverse clinical outcomes in severe COVID-19 patients

Haibo Li, Lili Ren, Lulu Zhang, Yeming Wang, Li Guo, Conghui Wang, Yan Xiao, Ying Wang, Jian Rao, Xinming Wang, Ying Liu, Chaolin Huang, Xiaoying Gu, Guohui Fan, Hui Li, Binghuai Lu, Bin Cao & Jianwei Wang
To identify the association between the kinetics of viral load and clinical outcome in severe coronavirus disease 2019 (COVID-19) patients, a retrospective study was performed by involved 188 hospitalized severe COVID-19 patients in the LOTUS China trial. Among the collected 578 paired throat swab (TS) and anal swab (AS) samples, viral RNA was detected in 193 (33.4%) TS and 121 (20.9%) AS. A higher viral RNA load was found in TS than that of AS,...

Evaluation of an enclosed air-lift photobioreactor (ALPBR) for biomass and lipid biosynthesis of microalgal cells grown under fluid-induced shear stress

Ning Ding, Chao Li, Tao Wang, Meijin Guo, Ali Mohsin & Siliang Zhang
An enclosed air-lift photobioreactor (ALPBR) is considered an efficient lab-scale bioreactor for microalgae cell growth and lipid biosynthesis. However, fluid-induced shear stress and mixing are two main factors that affect physiological metabolism in microalgal cell cultures. Herein, a 50-L ALPBR after being designed and manufactured was evaluated for microalgal suspension culture. Moreover, computational fluid dynamics (CFD) simulation was used to characterize the hydrodynamics of ALPBR. Specifically, two model microalgae species Chlorella vulgaris and Chlorella protothecoides...

Biogeography and ecological niche evolution in Diapensiaceae inferred from phylogenetic analysis

Michelle Gaynor, Chao-Nan Fu, Lian-Ming Gao, Li-Min Lu, Douglas E. Soltis & Pamela S. Soltis
Diapensiaceae (Ericales) are a small family of about 15 species. Within this clade, two species are broadly distributed throughout the Northern Hemisphere, while the remaining species have a disjunct distribution between eastern North America and eastern Asia. To address patterns and processes of diversification in Diapensiaceae, we conducted biogeographic analyses and inferred shifts in ecological niche across the phylogeny of the clade. Although Diapensiaceae have been the focus of multiple phylogenetic and biogeographic studies, previous...

Supplemental materials of: Ignored biodiversity in acid soil islands in karst areas, South China: Impatiens longlinensis (Balsaminaceae), a new critically endangered species

Tian-Tian Xue, Chang-Ying Xia, Magnus Lidén, Wei-Bin Xu, Zhao-Cen Lu, Hai-Ling Chen, Shu-Wan Li & Sheng-Xiang Yu
The karst area in South China is notable for its fragile vegetation and unique flora with many narrow endemics, and is one of the most threatened biodiversity hotspots in the world. However, the biodiversity of acid soil areas scattered in the karst area have previously been underestimated. With a recent increase of new discoveries of plants and animals, the conservation of biodiversity in these acid soil areas has become urgent. This study deals with a...

Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells

Hua-Long Xiong, Yang-Tao Wu, Jia-Li Cao, Ren Yang, Ying-Xia Liu, Jian Ma, Xiao-Yang Qiao, Xiang-Yang Yao, Bao-Hui Zhang, Ya-Li Zhang, Wang-Heng Hou, Yang Shi, Jing-Jing Xu, Liang Zhang, Shao-Juan Wang, Bao-Rong Fu, Ting Yang, Sheng-Xiang Ge, Jun Zhang, Quan Yuan, Bao-Ying Huang, Zhi-Yong Li, Tian-Ying Zhang & Ning-Shao Xia
The global pandemic of coronavirus disease 2019 (COVID-19) is a disaster for human society. A convenient and reliable neutralization assay is very important for the development of vaccines and novel drugs. In this study, a G protein-deficient vesicular stomatitis virus (VSVdG) bearing a truncated spike protein (S with C-terminal 18 amino acid truncation) was compared to that bearing the full-length spike protein of SARS-CoV-2 and showed much higher efficiency. A neutralization assay was established based...

SARS-CoV-2 spike produced in insect cells elicits high neutralization titres in non-human primates

Tingting Li, Qingbing Zheng, Hai Yu, Dinghui Wu, Wenhui Xue, Hualong Xiong, Xiaofen Huang, Meifeng Nie, Mingxi Yue, Rui Rong, Sibo Zhang, Yuyun Zhang, Yangtao Wu, Shaojuan Wang, Zhenghui Zha, Tingting Chen, Tingting Deng, Yingbin Wang, Tianying Zhang, Yixin Chen, Quan Yuan, Qinjian Zhao, Jun Zhang, Ying Gu, Shaowei Li … & Ningshao Xia
The current coronavirus disease 2019 (COVID-19) pandemic was the result of the rapid transmission of a highly pathogenic coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), for which there is no efficacious vaccine or therapeutic. Toward the development of a vaccine, here we expressed and evaluated as potential candidates four versions of the spike (S) protein using an insect cell expression system: receptor binding domain (RBD), S1 subunit, the wild-type S ectodomain (S-WT), and the...

SARS-CoV-2 spike produced in insect cells elicits high neutralization titres in non-human primates

Tingting Li, Qingbing Zheng, Hai Yu, Dinghui Wu, Wenhui Xue, Hualong Xiong, Xiaofen Huang, Meifeng Nie, Mingxi Yue, Rui Rong, Sibo Zhang, Yuyun Zhang, Yangtao Wu, Shaojuan Wang, Zhenghui Zha, Tingting Chen, Tingting Deng, Yingbin Wang, Tianying Zhang, Yixin Chen, Quan Yuan, Qinjian Zhao, Jun Zhang, Ying Gu, Shaowei Li … & Ningshao Xia
The current coronavirus disease 2019 (COVID-19) pandemic was the result of the rapid transmission of a highly pathogenic coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), for which there is no efficacious vaccine or therapeutic. Toward the development of a vaccine, here we expressed and evaluated as potential candidates four versions of the spike (S) protein using an insect cell expression system: receptor binding domain (RBD), S1 subunit, the wild-type S ectodomain (S-WT), and the...

Convergence of carbapenem resistance and hypervirulence in a highly-transmissible ST11 clone of K. pneumoniae: An epidemiological, genomic and functional study

Ping Li, Qiqiang Liang, Wugao Liu, Beiwen Zheng, Lizhang Liu, Wei Wang, Zhijiang Xu, Man Huang & Youjun Feng
Co-occurrence of hypervirulence and KPC-2 carbapenem resistant phenotypes in a highly-transmissible ST11 clone ofKlebsiella pneumoniae has elicited deep concerns from public health stand point. To address this puzzle, we conducted a large-scale epidemiological, clinical and genomic study of K. pneumonia ST11 clones with both hypervirulence and carbapenem resistance in two tertiary hospitals in Zhejiang province. Most of the patients (15/23) were diagnosed with exclusively carbapenem-resistant K. pneumoniae (CRKP) infections. Ten death cases were reported, some...

Long non-coding RNA XIST promotes the progression of esophageal squamous cell carcinoma through sponging miR-129-5p and upregulating CCND1 expression

Haoran Wang, Haomiao Li, Yongkui Yu, Qingfeng Jiang, Ruixiang Zhang, Haibo Sun, Wenqun Xing & Yin Li
Long non-coding RNA (lncRNA) X inactive specific transcript (XIST) has been identified as an oncogenic lncRNA in a series of human cancers, including esophageal squamous cell carcinoma (ESCC). In this study, we aimed to further explore the underlying mechanism of XIST on ESCC progression. qRT-PCR assay was used to determine the levels of XIST and miR-129-5p. Western blot analysis was performed to assess cyclin D1 (CCND1) expression. Bioinformatic analysis was performed using starBase v2.0 software....

Evaluation of an enclosed air-lift photobioreactor (ALPBR) for biomass and lipid biosynthesis of microalgal cells grown under fluid-induced shear stress

Ning Ding, Chao Li, Tao Wang, Meijin Guo, Ali Mohsin & Siliang Zhang
An enclosed air-lift photobioreactor (ALPBR) is considered an efficient lab-scale bioreactor for microalgae cell growth and lipid biosynthesis. However, fluid-induced shear stress and mixing are two main factors that affect physiological metabolism in microalgal cell cultures. Herein, a 50-L ALPBR after being designed and manufactured was evaluated for microalgal suspension culture. Moreover, computational fluid dynamics (CFD) simulation was used to characterize the hydrodynamics of ALPBR. Specifically, two model microalgae species Chlorella vulgaris and Chlorella protothecoides...

CDK1-PLK1/SGOL2/ANLN pathway mediating abnormal cell division in cell cycle may be a critical process in hepatocellular carcinoma

Ling Li, Kang Huang, Huijia Zhao, Binyao Chen, Qifa Ye & Jiang Yue
This study aims to investigate the potential mechanisms and identify core biomarkers of Hepatocellular carcinoma (HCC). The profile GSE113850 was downloaded to analyze the differentially expressed genes. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein–protein interaction network analysis were used to reveal the main signal pathways of the differentially expressed genes (DEGs) and hub genes. The correlation between core gene expression and pathological stages, the disease-free survival analysis, the overall survival...

Convergence of carbapenem resistance and hypervirulence in a highly-transmissible ST11 clone of K. pneumoniae: An epidemiological, genomic and functional study

Ping Li, Qiqiang Liang, Wugao Liu, Beiwen Zheng, Lizhang Liu, Wei Wang, Zhijiang Xu, Man Huang & Youjun Feng
Co-occurrence of hypervirulence and KPC-2 carbapenem resistant phenotypes in a highly-transmissible ST11 clone ofKlebsiella pneumoniae has elicited deep concerns from public health stand point. To address this puzzle, we conducted a large-scale epidemiological, clinical and genomic study of K. pneumonia ST11 clones with both hypervirulence and carbapenem resistance in two tertiary hospitals in Zhejiang province. Most of the patients (15/23) were diagnosed with exclusively carbapenem-resistant K. pneumoniae (CRKP) infections. Ten death cases were reported, some...

Longitudinal analysis of the antibody repertoire of a Zika virus-infected patient revealed dynamic changes in antibody response

Xuefeng Niu, Qihong Yan, Zhipeng Yao, Fan Zhang, Linbing Qu, Chunlin Wang, Chengrui Wang, Hui Lei, Chaoming Chen, Renshan Liang, Jia Luo, Qian Wang, Lingzhai Zhao, Yudi Zhang, Kun Luo, Longyu Wang, Hongkai Wu, Tingting Liu, Pingchao Li, Zhiqiang Zheng, Yee Joo Tan, Liqiang Feng, Zhenhai Zhang, Jian Han, Fuchun Zhang … & Ling Chen
The Zika virus (ZIKV) is a mosquito-borne flavivirus that causes neonatal abnormalities and other disorders. Antibodies to the ZIKV envelope (E) protein can block infection. In this study, next-generation sequencing (NGS) of immunoglobulin heavy chain (IgH) mRNA transcripts was combined with single-cell PCR cloning of E-binding monoclonal antibodies for analysing antibody response in a patient from the early stages of infection to more than one year after the clearance of the virus. The patient's IgH...

CDK1-PLK1/SGOL2/ANLN pathway mediating abnormal cell division in cell cycle may be a critical process in hepatocellular carcinoma

Ling Li, Kang Huang, Huijia Zhao, Binyao Chen, Qifa Ye & Jiang Yue
This study aims to investigate the potential mechanisms and identify core biomarkers of Hepatocellular carcinoma (HCC). The profile GSE113850 was downloaded to analyze the differentially expressed genes. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein–protein interaction network analysis were used to reveal the main signal pathways of the differentially expressed genes (DEGs) and hub genes. The correlation between core gene expression and pathological stages, the disease-free survival analysis, the overall survival...

Profiling serum cytokines in COVID-19 patients reveals IL-6 and IL-10 are disease severity predictors

Huan Han, Qingfeng Ma, Cong Li, Rui Liu, Li Zhao, Wei Wang, Pingan Zhang, Xinghui Liu, Guosheng Gao, Fang Liu, Yingan Jiang, Xiaoming Cheng, Chengliang Zhu & Yuchen Xia
Since the outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, China, it has rapidly spread across many other countries. While the majority of patients were considered mild, critically ill patients involving respiratory failure and multiple organ dysfunction syndrome are not uncommon, which could result death. We hypothesized that cytokine storm is associated with severe outcome. We enrolled 102 COVID-19 patients who were admitted to Renmin Hospital (Wuhan, China). All patients were classified into moderate, severe...

Data from: Plant quantity and quality regulate the diversity of arthropod communities in a semi-arid grassland

Xiaoming Lu, Xuezhen Zhao, Taiki Tachibana, Kei Uchida, Takehiro Sasaki & Yongfei Bai
The quantity (e.g. biomass production) and quality (e.g. leaf nutrient content) of plants can strongly influence arthropod diversity, but few studies have tried to disentangle such effects. In this study, we examined the independent effects of plant productivity and leaf traits on the taxon richness and abundance of entire arthropod communities and multiple arthropod orders in replicated monocultures of 15 herbaceous species in the Inner Mongolian grassland. Total taxon richness of arthropod communities increased with...

High anal swab viral load predisposes adverse clinical outcomes in severe COVID-19 patients

Haibo Li, Lili Ren, Lulu Zhang, Yeming Wang, Li Guo, Conghui Wang, Yan Xiao, Ying Wang, Jian Rao, Xinming Wang, Ying Liu, Chaolin Huang, Xiaoying Gu, Guohui Fan, Hui Li, Binghuai Lu, Bin Cao & Jianwei Wang
To identify the association between the kinetics of viral load and clinical outcome in severe coronavirus disease 2019 (COVID-19) patients, a retrospective study was performed by involved 188 hospitalized severe COVID-19 patients in the LOTUS China trial. Among the collected 578 paired throat swab (TS) and anal swab (AS) samples, viral RNA was detected in 193 (33.4%) TS and 121 (20.9%) AS. A higher viral RNA load was found in TS than that of AS,...

Registration Year

  • 2020
    24

Resource Types

  • Dataset
    10
  • Text
    10
  • Image
    4

Affiliations

  • Institute of Botany
    24
  • Zhejiang University
    17
  • Sichuan University
    17
  • Shanghai Jiao Tong University
    17
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    14
  • Central South University
    13
  • Fudan University
    12
  • West China Hospital of Sichuan University
    11
  • Huazhong University of Science and Technology
    11
  • Ministry of Education of the People's Republic of China
    10