Additional file 6: Fig. S6 Concordance of ctDNA response and radiographic response.

Additional file 7: Fig. S7 Mutational profiles of CSF and plasma samples at baseline.

Additional file 8: Fig. S8 The proportion of clonal and subclonal mutations in CSF and plasma samples at baseline and after 8 weeks of treatment.

Additional file 1: Fig. S1. Study participant flow diagram.

Supplementary Material 1

Additional file 1: Figure S1. General comparison of immune markers between primary tumors and liver metastases (A: CD8, B: CD20, C: CD68, D: CD11c, E: VEGFR-2, F: PD-L1, G: Ki67) (p ≥ 0.05, not significant).

Additional file 7: Figure S4. Comparison of the three immunotypes according to the degree of CD8 infiltration. A: Comparison of the three immunotypes at the front of the tumor (CD68, Foxp3, PD-L1 and Ki67). C: Comparison of the three immunotypes at the center of the tumor (CD68, Foxp3, PD-L1 and Ki67). (*p< 0.05, p ≥ 0.05, not significant).

Abstract Background Liver metastases are a major contributor to the poor immunotherapy response in colorectal cancer patients. However, the distinctions in the immune microenvironment between primary tumors and liver metastases are poorly characterized. The goal of this study was to compare the expression profile of multiple immune cells to further analyze the similarities and differences between the microenvironments of liver metastases and the primary tumor. Methods Tissues from 17 patients with colorectal cancer who underwent...

Abstract In oral and maxillofacial surgery, flap repair is essential to the quality of postoperative life. Still, thrombosis is fatal for the survival of the flaps. Besides, some postoperative thrombotic diseases, such as pulmonary embolism, also intimidate patients’ life. The traditional diagnostic methods are still limited by a large amount of hardware and suffer from inconvenience, delay, and subjectivity. Moreover, the treatments mainly rely upon thrombolytics, such as urokinase (UK) plasminogen activator, which may cause...

Phytochemical investigation of the seeds of Capsella bursa-pastoris led to the isolation of four organosulfur compounds. There were two new compounds, 10-methylsulfinyl-decanamide (1) and 11-methylsulfinyl-undecanamide (2), along with two known compounds (3 − 4), which all have a sulfoxide group and an amide or a nitrile group. Their chemical structures were elucidated by analysing UV, IR, ESI-MS and NMR spectroscopy. In addition, compounds 1 − 4 were evaluated for their anti-inflammatory activities by using LPS-induced...

Additional file 1: Figure S1. Vessel densities of the whole measured area, parafovea, and four quadrants (temporal, superior, nasal, and inferior) in the (a, c) SCP and (b, d) DCP measured with optical coherence tomography angiography stratified by age in males and females. Table S1. Baseline characteristics of male participants in the study. Table S2. Baseline characteristics of female participants in the study. Table S3. Vessel densities of the whole measured area, parafovea, and four...

Nowadays, early defect detection plays a significant role for the railway safety warning. However, the existing methods cannot satisfy the requirements of real-time and high-precision detection. Here, a high-precision, distributed and on-line method for detecting rail defect is proposed and demonstrated. When a train goes through defects, the instantaneous elastic waves will be excited by the wheel-rail interaction, which will further propagate along railway tracks bidirectionally. Through mounting the backscattering enhanced optical fiber on the...

Supplementary Material 2

supplemental document of the manuscript

Additional file 16. Representation of the number of genes differentially expressed in the medial preoptic area (MPOA, top), nucleus accumbens (NAc, middle), and lateral septum (LS, bottom) across all chromosomes and linkage groups in the prairie vole assembly. Only cases with at least one differentially expressed gene are depicted.

Additional file 17. Functional enrichment of genes differentially expressed in the nucleus accumbens (NAc). For each pairwise comparison, the functional enrichments in gene ontologies of the biological processes (A), cellular components (B), and molecular functions (C) categories as well as in pathways from the Kyoto Encyclopedia of Genes and Genomes (KEGG, D) were tested for the differentially expressed genes up- or down-regulated (UP, and DOWN, respectively). The labels of pairwise comparisons were coded in two...

Additional file 22. Structure-specific associations of gene coexpression modules with parental behaviors. The correlation of each co-expression module from the weighted gene coexpression network analysis with behavioral traits (behaviors scored during parental behavior test and phenotype status) is depicted for each structure. The correlation value is detailed alongside its corresponding p-value in parentheses. Note that within each structure, only modules with at least one significant association are depicted. MPOA: medial preoptic area, NAc: nucleus accumbens,...

Additional file 24. Functional enrichment of gene ontologies of the biological processes category in gene co-expression modules derived from the weighted gene coexpression network analysis (WGCNA).

Additional file 2: Clinical stage-specific disability weights and distribution of breast cancer in females in China.

Additional file 4: Age-specific number of females and standardized life expectancy

Abstract Objectives ST11 KPC-producing Klebsiella pneumoniae (Kp) is highly prevalent in China. We investigated the inter- and intra- host transmission and evolution characteristics of ST11 KPC-producing Kp. Methods A retrospective study was conducted in a hospital. The clinical data and antimicrobial resistance (AMR) phenotypes were collected. Whole genome sequencing was performed. The transmission route was reconstructed by combining single nucleotide polymorphisms (SNPs) with the clinical information. Hypervirulent Kp (HvKp) was defined as the presence of...

Additional file 1: Figure S1. Collection date of these ST11-Kp strains. Figure S2. Specimen type of these ST11-Kp strains. Table S1. Antimicrobial resistance profile of these ST11-Kp strains.

Supplementary Material 1: Table S1 Genome sequence table

Abstract Background Many clinical studies have reported the high success rate of the All-on-4 concept. In the present study, we aimed to compare the stress distribution with different tilted distal implants and cantilever lengths in an All-on-4 system using the two-dimensional photoelastic method and to establish the All-on-4 implant photoelastic model by computer-aided design (CAD) and rapid prototyping (RP). Methods The data of the human edentulous mandible were acquired by computed tomography (CT). Three human...

Identifying the latent cluster structure based on model heterogeneity is a fundamental but challenging task arises in many machine learning applications. In this article, we study the clustered coefficient regression problem in the distributed network systems, where the data are locally collected and held by nodes. Our work aims to improve the regression estimation efficiency by aggregating the neighbors’ information while also identifying the cluster membership for nodes. To achieve efficient estimation and clustering, we...