5,150 Works

Transcriptome analysis revealing molecular mechanisms of enhanced pigment yield by succinic acid and fluconazole

Jie Qiao, Xuanxuan He, Chengtao Wang, Huilin Yang, Zeng Xin, Binyue Xin, Junnan Wang, Ruoyun Dong, Huawei Zeng & Feng Li
This study aimed to elucidate the molecular mechanisms through which succinic acid and fluconazole stimulate Monascus pigment biosynthesis under liquid fermentation conditions. The pigment yield was significantly improved by adding 0.35 g·L−1 succinic acid or 1.5 g·L−1 fluconazole. Transcriptome sequencing and RT-qPCR confirmation were performed to reveal transcriptome changes. The results indicated that the addition of succinic acid significantly decreased mRNA expression of genes involved in fatty acid biosynthesis while increasing expression of genes involved...

Polygonum cuspidatum inhibits the growth of osteosarcoma cells via impeding Akt/ERK/EGFR signaling pathways

Jun Zhao, Boyu Pan, Xinglu Zhou, Chunnuan Wu, Fengcheng Hao, Jie Zhang & Liren Liu
Osteosarcoma (OS), the most prevalent bone malignancy, mainly affects children and adolescents. Despite recent advances in multimodal therapy, the overall survival rate for OS patients remains poor. Chinese herb medicine (CHM) is an alternative therapeutic option for multifaceted diseases such as malignant tumors. For centuries in China, polygonum cuspidatum, a classic CHM, has been used to treat several diseases, nevertheless, the mechanisms underlying its therapeutic effects have not been fully elucidated. Through network pharmacology and...

miR-669a-5p promotes adipogenic differentiation and induces browning in preadipocytes

Xiaoqiong Tan, Tingting Zhu, Linqiang Zhang, Lin Fu, Ying Hu, Huiqin Li, Chengbin Li, Jingjing Zhang, Bin Liang & Jing Liu
Obesity is a major global health issue that contributes to the occurrence of metabolic disorders. Based on this fact, understanding the underlying mechanisms and to uncover promising therapeutic approaches for obesity have attracted intense investigation. Brown adipose tissue (BAT) can help burns excess calories. Therefore, promoting White adipose tissue (WAT) browning and BAT activation is an attractive strategy for obesity treatment. MicroRNAs (miRNAs) are small, non-coding RNAs, which are involved in regulation of adipogenic processes...

Safety, mucosal and systemic immunopotency of an aerosolized adenovirus-vectored vaccine against SARS-CoV-2 in rhesus macaques

Fang Xu, Shipo Wu, Linan Yi, Shaodan Peng, Fan Wang, Weixue Si, Lihua Hou & Tao Zhu
Mucosal immunity provides a potential for preventing initial infection and stopping subsequent transmission of SARS-CoV-2. Here, we examined the safety and immunogenicity of a replication-defective adenovirus type-5 vectored vaccine (Ad5-nCov) encoding SARS-CoV-2 spike protein delivered by nebulization inhalation in rhesus macaques. The vaccine-associated clinical pathology and toxicity were not observed in the NHP model. The extensive safety study indicated that Ad5-nCoV was mainly confined to the organs related to respiratory system and was rapidly cleared...

Safety, mucosal and systemic immunopotency of an aerosolized adenovirus-vectored vaccine against SARS-CoV-2 in rhesus macaques

Fang Xu, Shipo Wu, Linan Yi, Shaodan Peng, Fan Wang, Weixue Si, Lihua Hou & Tao Zhu
Mucosal immunity provides a potential for preventing initial infection and stopping subsequent transmission of SARS-CoV-2. Here, we examined the safety and immunogenicity of a replication-defective adenovirus type-5 vectored vaccine (Ad5-nCov) encoding SARS-CoV-2 spike protein delivered by nebulization inhalation in rhesus macaques. The vaccine-associated clinical pathology and toxicity were not observed in the NHP model. The extensive safety study indicated that Ad5-nCoV was mainly confined to the organs related to respiratory system and was rapidly cleared...

A metal tolerance protein, MTP10, is required for the calcium and magnesium homeostasis in Arabidopsis

Haiman Ge, Qiaolin Shao, Jinlin Chen, Jiahong Chen, Xueqin Li, Yu Tan, Wenzhi Lan, Lei Yang & Yuan Wang
Nutrient antagonism typically refers to the fact that too high a concentration of one nutrient inhibits the absorption of another nutrient. In plants, Ca2+ (Calcium) and Mg2+ (Magnesium) are the two most abundant divalent cations, which are known to have antagonistic interactions. Hence, maintaining their homeostasis is crucial for plant growth and development. In this study, we showed that MTP10 (Metal Tolerance Protein 10) is an important regulator for maintaining homeostasis of Mg and Ca...

MYB proto-oncogene like 2 promotes hepatocellular carcinoma growth and glycolysis via binding to the Optic atrophy 3 promoter and activating its expression

Miao Liu, Qiang Du, Gang Mao, Ning Dai & Fan Zhang
Optic atrophy 3 (OPA3) is an integral protein of the mitochondrial outer membrane. The current study explored the expression of OPA3 in hepatocellular carcinoma (HCC), its association with the prognosis and its involvement in HCC cell proliferation and aerobic glycolysis. In addition, the transcription factors that activate its expression were screened and validated. Gene expression data in normal liver and liver cancer were acquired from the Genotype-Tissue Expression Project (GTEx) and The Cancer Genome Atlas...

MYB proto-oncogene like 2 promotes hepatocellular carcinoma growth and glycolysis via binding to the Optic atrophy 3 promoter and activating its expression

Miao Liu, Qiang Du, Gang Mao, Ning Dai & Fan Zhang
Optic atrophy 3 (OPA3) is an integral protein of the mitochondrial outer membrane. The current study explored the expression of OPA3 in hepatocellular carcinoma (HCC), its association with the prognosis and its involvement in HCC cell proliferation and aerobic glycolysis. In addition, the transcription factors that activate its expression were screened and validated. Gene expression data in normal liver and liver cancer were acquired from the Genotype-Tissue Expression Project (GTEx) and The Cancer Genome Atlas...

Discovery of novel paeonol-based derivatives against skin inflammation in vitro and in vivo

Jing Wu, Ren De Zhu, Guo Min Cao, Jun Cheng Du, Xin Liu, Liang Zhuo Diao, Zhao Yan Zhang, Yang Sheng Hu, Xin Hua Liu & Jing Bo Shi
T-LAK-cell-originated protein kinase (TOPK), a novel member of the mitogen-activated protein kinase family, is considered an effective therapeutic target for skin inflammation. In this study, a series (A − D) of paeonol derivatives was designed and synthesised using a fragment growing approach, and their anti-inflammatory activities against lipopolysaccharide (LPS)-induced nitric oxide production in RAW264.7 cells were tested. Among them, compound B12 yielded the best results (IC50 = 2.14 μM) with low toxicity (IC50 > 50...

Controlled synthesis of cube-like ZnSnO3 decorated by nickel-based films and electrochemical applications on lithium-sulfur batteries

Mianjiao Jiang, Jun Wu, Hongmei Guo, Hongfang Zhang, Manli Zhu & Xiaobang Xu
Transition metal oxide was considered as an effective cathode additive to depress ‘shuttle effect’ in lithium-sulfur (Li-S) batteries via forming a strong polar chemical bond with polysulfides. Here, we firstly synthesized unique S@Ni/NiO/ZnSnO3 microcubes as composite cathodes for Li-S batteries, in which Ni/NiO films were deposited onto surface of as-prepared ZnSnO3 microcubes via a hydrothermal reaction and later calcinations process. The S@Ni/NiO/ZnSnO3 composite showed attractive electrochemical performances with a high initial discharge-specific capacity of 1070...

Injection of YiQiFuMai powder protects against heart failure via inhibiting p38 and ERK1/2 MAPKs activation

Yongwei Nie, Yanxin Zhang, Zhi Li, Meixu Wan & Dekun Li
Injection of YiQiFuMai (YQFM) powder, a modern Chinese plant-derived medical preparation, has a therapeutic effect in heart failure (HF). However, its therapeutic mechanism remains largely unknown. To investigate the molecular mechanisms of YQFM in HF. Kinase inhibition profiling assays with 2 mg/mL YQFM were performed against a series of 408 kinases. In addition, the effects of kinase inhibition were validated in cardiomyocyte cell line H9c2. In vivo, HF with reduced ejection fraction (HFrEF) was induced...

Long non-coding RNA TRPM2 antisense RNA as a potential therapeutic target promotes tumorigenesis and metastasis in esophageal cancer

Wei Wang, Yukai Dai, Xin Yang & Xinming Xiong
Esophageal cancer (EC) is one type of aggressive gastrointestinal cancers. The treatment of EC is challenging. Effective therapeutic targets require development. Long non-coding RNA TRPM2 antisense RNA (LncRNA TRPM2-AS) is considering a novel biomarker and therapeutic target for various types of cancer. However, the role of lncRNA TRPM2-AS in EC remains unknown. This study aimed to illustrate effects of LncRNA TRPM2-AS on EC growth and metastasis and potential underlying molecular mechanisms. LncRNA TRPM2-AS expression was...

Exosomes from miR-374a-5p-modified mesenchymal stem cells inhibit the progression of renal fibrosis by regulating MAPK6/MK5/YAP axis

Mingzhu Liang, Di Zhang, Danna Zheng, Wenfang He & Juan Jin
Chronic kidney disease (CKD) in clinical is defined as a gradual loss of kidney function for more than 3 months. The pathologic course of CKD is characterized by extensive renal fibrosis; thus, preventing renal fibrosis is vital for the treatment of CKD. It has been reported that microRNA (miR)-374a-5p was under-expressed in renal venous blood samples from patients with CKD. In addition, it exhibited anti-apoptotic effects in renal tissues suggesting that miR-374a-5p may play an...

Long noncoding RNA H19 suppresses cardiac hypertrophy through the MicroRNA-145-3p/SMAD4 axis

Hao Wang, Xiaoqing Lian, Wei Gao, Jie Gu, Haojie Shi, Yao Ma, Yafei Li, Yi Fan, Qiming Wang & Liansheng Wang
Sustained cardiac hypertrophy (CH) contributes to many heart diseases. Long noncoding RNAs (lncRNAs) collectively play critical roles in cardiovascular diseases (CVDs). However, the roles of lncRNA H19 in CH are still unclear. A CH model was constructed utilizing isoproterenol (ISO). We demonstrated H19 could participate in regulating ISO-induced CH development both in vivo and in vitro. The online databases DIANA and TargetScan were used to predict the targets of H19 and MicroRNA-145-3p (miR-145-3p), respectively. Luciferase...

Upstream stimulatory factor 2 (USF2) induced upregulation of triggering receptor expressed on myeloid cells 1 (TREM1) promotes endometritis by regulating toll-like receptor (TLR) 2/4-nuclear factor-kappaB (NF-κB) signaling pathway

Miao Zhang, Chengkun Yin, Yan Chen, Juan Wang & Jing Jiang
Triggering receptor expressed on myeloid cells 1 (TREM1) participates in the development of endometritis. This study aims at identifying the effects and interaction of TREM1 and upstream stimulatory factor 2 (USF2) in endometritis by using a model of lipopolysaccharide (LPS)-induced human endometrial epithelial cells (HEnEpCs). ELISA was performed to determine the levels of interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF-α) after LPS stimulation. TREM1 and USF2 expression was examined with RT-qPCR and Western blot....

Numerical simulation of ball bearing flow field using the moving particle semi-implicit method

Wei Wu, Chunhui Wei & Shihua Yuan
The distribution of lubricating oil in the bearing cavity is of great significance to bearing lubrication and cooling. A new idea is provided to further study the ball bearings lubrication, to achieve effective lubrication of bearings. The flow field of oil injection lubrication ball bearings is studied by the moving particle semi-implicit (MPS) method. The accuracy of the numerical calculation method is verified by experiments. The oil distribution of the bearing lubrication flow field and...

The Effect of the Acidic Gas Pollutant on Wool Keratin Fiber Structure

Zhe Jiang, Rubing Bai, Jinfang Zhang, Ende Li, Yifan Cui, Ning Zhang, Nan Zhang & Qiang Wang
Increase in air pollution caused by acidic gases, such as nitrogen dioxide, call for the need to study potential effects on the structure of textile materials. Wool is a natural protein fiber which is easily damaged due to rich reaction positions and cross-linked bonds in the fiber. This study reports, for the first time, effect of nitrogen dioxide, as a model acid gas pollutant, on the structure of wool. On the macro level, wool fabric...

Protein disulfide isomerases (PDIs) negatively regulate ebolavirus structural glycoprotein expression in the endoplasmic reticulum (ER) via the autophagy-lysosomal pathway

Bin Wang, Jing Zhang, Xin Liu, Qingqing Chai, Xiaoran Lu, Xiaoyu Yao, Zhichang Yang, Liangliang Sun, Silas F. Johnson, Richard C Schwartz & Yong-Hui Zheng
Zaire ebolavirus (EBOV) causes a severe hemorrhagic fever in humans and non-human primates with high morbidity and mortality. EBOV infection is dependent on its structural glycoprotein (GP), but high levels of GP expression also trigger cell rounding, detachment, and downregulation of many surface molecules that is thought to contribute to its high pathogenicity. Thus, EBOV has evolved an RNA editing mechanism to reduce its GP expression and increase its fitness. We now report that the...

Has_circ_0008583 modulates hepatocellular carcinoma progression through the miR-1301-3p/METTL3 pathway

Fenfen Wang, Zhengyuan Xie, Nuobei Zhang, Hao Ding, Kai Xiong, Li Guo, Hongyan Huang & Zhili Wen
Has_circ_0008583 is reported to be involved in the progression of hepatocellular carcinoma (HCC), while its biological role in HCC remains unclear. Here, the qRT-PCR was used to detect the expression of has_circ_0008583. The CCK-8 kit was performed to measure cell proliferation. The cell migration and invasion were evaluated by Transwell. A dual-luciferase reporter assay was performed to confirm the target combination between the genes in has_circ_0008583/miR-1301-3p/METTL3 axis. The in vivo role of has_circ_0008583 was verified...

Numerical investigation of asymmetric weld fusion geometry in laser welding of aluminium alloy with beam oscillation

Xi Chen, Nan Jiang, Meng Jiang, Yang Du, Shengchong Ma, Yuan Chen, Caiwang Tan, Zhenglong Lei, Sicong Zhao & Yanbin Chen
In this work, the asymmetric weld fusion geometry in oscillating laser beam welding (OLBW) of aluminium alloy was reported and investigated using a numerical approach. A multi-physics heat transfer and fluid flow model of OLBW was developed and validated with the corresponding experimental results. The weld fusion geometry, temperature fields, and fluid flow behaviours for four commonly used oscillation modes, line, circle, eight, and infinity, were calculated to analyse the origin of the asymmetric weld...

Rapid Qualitative and Quantitative Characterization of Arnebiae Radix by Near-Infrared Spectroscopy (NIRS) with Partial Least Squares—Discriminant Analysis (PLS-DA)

Na Zhao, Zhaoyang Li, Youping Li, Gaixia Liu, Xiling Deng, Qian Ma, Chenglin Hong & Shiguo Sun
Near infrared (NIR) spectroscopy with chemometrics was used for rapid source identification and component quantification of Arnebiae Radix. The modeling parameters were determined using the processing trajectory method. The quantitative model of target components was established using the model fusion method. The root mean square error of calibration (RMSEC), root mean square error of validation (RMSEP), determination coefficient of calibration (Rcal2) and validation (Rpre2), and the ratio of standard error of prediction to standard deviation...

Ultrastructure change and transcriptome analysis of GA3 treatment on seed germination of moso bamboo(Phyllostachys edulis)

Juan Li, Yucong Bai, Yali Xie & Jian Gao
Exploring the mechanism of Gibberellic acid (GA3) treatment on seed germination of moso bamboo can lay a foundation for its future breeding and research. In this study, the germination-related indicators (germination rate, germination potential, vigor index, respiration rate) with different content of GA3 treatment were measured, and the ultrastructure of moso bamboo seeds treated with low and high GA3 concentrations was observed during the germination process. In addition, the transcriptome data of the germination seeds,...

FROM-GLC Plus: toward near real-time and multi-resolution land cover mapping

Le Yu, Zhenrong Du, Runmin Dong, Juepeng Zheng, Ying Tu, Xin Chen, Pengyu Hao, Bo Zhong, Dailiang Peng, Jiyao Zhao, Xiyu Li, Jianyu Yang, Haohuan Fu, Guangwen Yang & Peng Gong
Global land cover has undergone extensive and rapid changes as a result of human activities and climate change. These changes have had a significant impact on biodiversity, the surface energy balance, and sustainable development. Global land cover data underpins research on the development of earth system models, resource management, and evaluation of the ecological environment. However, there are limitations in the classification detail, spatial resolution, and rapid change monitoring capability of global land cover change...

Optimization of the vancomycin administration regimen by clinical pharmacists based on a population pharmacokinetics model: a prospective interventional study

Haodi Lu, Lufen Duan, Yanxia Yu, Jingjing Li, Lu Shi, Sudong Xue, Qian Zhang, Qin Zhou, Chenqi Zhu, Erning Shang, Xinxin Yan & Lian Tang
In vancomycin treatment, the rates of correct blood sampling and initial trough concentrations within the target range are very low. Studies of interventions by clinical pharmacists based on population pharmacokinetics (PPK) models are limited. This study aimed to evaluate the intervention effect of clinical pharmacist-mediated optimization of the vancomycin administration regimen based on a PPK model. Retrospectively enrolled patients constituted the control group, and prospectively enrolled patients constituted the intervention group. The vancomycin administration regimen,...

Ultrastructure change and transcriptome analysis of GA3 treatment on seed germination of moso bamboo(Phyllostachys edulis)

Juan Li, Yucong Bai, Yali Xie & Jian Gao
Exploring the mechanism of Gibberellic acid (GA3) treatment on seed germination of moso bamboo can lay a foundation for its future breeding and research. In this study, the germination-related indicators (germination rate, germination potential, vigor index, respiration rate) with different content of GA3 treatment were measured, and the ultrastructure of moso bamboo seeds treated with low and high GA3 concentrations was observed during the germination process. In addition, the transcriptome data of the germination seeds,...

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