268 Works

Identification of microRNA hsa-miR-30c-5p as an inhibitory factor in the progression of hepatocellular carcinoma and investigation of its regulatory network via comprehensive analysis

Shangshang Hu, Jinyan Zhang, Xiaoyu Fang, Guoqing Guo, Jing Dai, Zhiyong Sheng, Dongdong Li, Jiasheng Chen, Li Zhang, Chuanmiao Liu & Yu Gao
Hepatocellular carcinoma (HCC) is a primary liver cancer with high morbidity and mortality. An increasing number of abnormal gene expressions were identified to be associated with the progression of HCC. Previous studies showed that the hsa-miR-30 c-5p (miR-30 c), one of the miR-30 family members, might play a role in suppressing tumor progression in a variety of tumors. The present study aims to examine miR-30 c effects in the development of HCC. The role of...

Comprehensive Analysis of Monocarboxylate Transporter 4 (MCT4) expression in breast cancer prognosis and immune infiltration via integrated bioinformatics analysis

Chen Yuan, Jie Zhang, Jianjuan Lou, Siqi Wang, Yanni Jiang, Feiyun Wu & Shouju Wang
Lactate blunts the anticancer immune response in breast cancer (BC). However, little is known about the exact effect of lactate transporters such as monocarboxylate transporter 4 (MCT4) on immunotherapy. In this study, we investigated the expression status and prognostic value of MCT4 in BC through large-scale transcriptome data. Our results showed that MCT4 was overexpressed in BC, particularly in the basal-like molecular subtype. Overexpression of MCT4 was significantly correlated with high BC lesion grade and...

Prediction of clinical prognosis in cutaneous melanoma using an immune-related gene pair signature

Yu Yang, Xuan Long, Guiyun Li, Xiaohong Yu, Yu Liu, Kun Li & Xiaobin Tian
Cutaneous melanoma (CM) is a malignant and aggressive skin cancer that is the leading cause of skin cancer-related deaths. Increasing evidence shows that immunity plays a vital role in the prognosis of CM. In this study, we developed an immune-related gene pair (IRGP) signature to predict the clinical prognosis of patients with CM. Immune-related genes from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases were selected to construct the IRGPs, and...

Special electromagnetic field-treated water and far-infrared radiation alleviates lipopolysaccharide-induced acute respiratory distress syndrome in rats by regulating haptoglobin

Changyong Luo, Yan Li, Xu Liang, Yifan Chen, Qiao Zou, Yurong Kong, Zhengguang Guo, Wei Sun & Xin Wang
Special electromagnetic field-treated water (SEW) and far-infrared radiation (FIR) can reduce acute respiratory distress syndrome (ARDS) in rats inflicted by lipopolysaccharides (LPSs). However, little is known about its underlying molecular mechanism. Differentially expressed proteins (DEPs) of SEW and FIR interventions were obtained from a proteomics database. A total of 89 DEPs were identified. Enrichment analysis of DEPs was performed using the Database for Annotation, Visualization, and Integrated Discovery. These DEPs were associated with the responses...

The effect of amiloride in decreasing albuminuria in patients with diabetic kidney diseases: a prospective, crossover, open-label study

Ruizhao Li, Zhiyong Xie, Li Zhang, Ying Huang, Jianchao Ma, Wei Dong, Zhilian Li, Yuanhan Chen, Huaban Liang, Yanhua Wu, Xingchen Zhao, Wenjian Wang, Zhiming Ye, Shuangxin Liu, Wei Shi & Xinling Liang
Diabetic kidney diseases (DKD) were the leading cause of End-stage renal diseases worldwide. Albuminuria was a target for treatment in DKD and decreasing albuminuria was particularly important for improving its prognosis. However, there is still a lack of specific treatment for DKD. We conducted a prospective, crossover, open-label study to investigate the effect of amiloride in patients with DKD. Safety and efficacy were assessed by monitoring urine protein creatinine ratio(uPCR), urinary albumin creatinine ratio (uACR),...

Diagnostic significance of microRNA-1255b-5p in prostate cancer patients and its effect on cancer cell function

Yuling Zhao, Xiaochun Tang, Yifan Zhao, Yan Yu & Shuzhen Liu
Discerning between indolent and aggressive types is a big challenge of prostate cancer clinically to guide the adequate therapeutic regimen. We aimed to examine the relationship between miR-1255b-p expression and prostate cancer and elucidate the function of miR-1255b-5p in prostate cancer. miR-1255b-5p were measured using Quantitative Real-Time PCR from the blood 103 benign prostate hyperplasia (BPH) and 153 prostate cancer patients (117 indolent cases and 36 upgrading cases). Using receiver operating characteristic (ROC) curve analysis,...

Tumor-targeted Gd-doped mesoporous Fe3O4 nanoparticles for T1/T2 MR imaging guided synergistic cancer therapy

Shaohui Zheng, Shang Jin, Min Jiao, Wenjun Wang, Xiaoyu Zhou, Jie Xu, Yong Wang, Peipei Dou, Zhen Jin, Changyu Wu, Jingjing Li, Xinting Ge & Kai Xu
In this study, a novel intelligent nanoplatform to integrate multiple imaging and therapeutic functions for targeted cancer theranostics. The nanoplatform, DOX@Gd-MFe3O4 NPs, was constructed Gd-doped mesoporous Fe3O4 nanoparticles following with the doxorubicin (DOX) loading in the mesopores of the NPs. The DOX@Gd-MFe3O4 NPs exhibited good properties in colloidal dispersity, photothermal conversion, NIR triggered drug release, and high T1/T2 relaxicity rate (r1=9.64 mM−1s−1, r2= 177.71 mM−1s−1). Benefiting from the high MR contrast, DOX@Gd-MFe3O4 NPs enabled simultaneous...

Folic acid-modified ROS-responsive nanoparticles encapsulating luteolin for targeted breast cancer treatment

Yu Wang, Qianmei Wang, Wei Feng, Qian Yuan, Xiaowei Qi, Sheng Chen, Pu Yao, Qing Dai, Peiyuan Xia, Dinglin Zhang & Fengjun Sun
Luteolin (Lut) is a natural flavonoid polyphenolic compound with multiple pharmacological activities, such as anti-oxidant, anti-inflammatory, and anti-tumor effects. However, the poor aqueous solubility and low bioactivity of Lut restrict its clinical translation. Herein, we developed a reactive oxygen species (ROS)-responsive nanoplatforms to improve the bioactivity of Lut. Folic acid (FA) was employed to decorate the nanoparticles (NPs) to enhance its targeting ability. The size of Lut-loaded ROS-responsive nanoparticles (Lut/Oxi-αCD NPs) and FA-modified Lut/Oxi-αCD NPs...

Silmitasertib-induced macropinocytosis promoting DDP intracellular uptake to enhance cell apoptosis in oral squamous cell carcinoma

Shaojuan Song, Xin Xia, Jiajia Qi, Xiaopei Hu, Qian Chen, Jiang Liu, Ning Ji & Hang Zhao
Cisplatin (DDP) is a first-line chemotherapeutic drug applied for the treatment of oral squamous cell carcinoma (OSCC). The anticancer activity of DDP is tightly linked to its intracellular uptake. It is unwise to increase the DDP intake by increasing the dose or shortening the dosing interval because of the severe systemic toxicity (nephrotoxicity, ototoxicity and neurotoxicity) in DDP application. The main uptake pathways of DDP include passive diffusion and active transporter transport. Therefore, finding additional...

Taberdines L and M, two new alkaloids from Tabernaemontana divaricata

Lingling Han, Kepu Huang, Chen Chen, Wentao Zhu, Yehan Ma, Xiaojiang Hao, Hongping He & Yu Zhang
Two new alkaloids, taberdines L (1) and M (2), together with six known alkaloids, were isolated from the twigs and leaves of Tabernaemontana divaricata. Taberdine L (1) represents the first optically active natural member of the allo iboga class of alkaloids with the ethyl side chain in a bridgehead position. Their structures including absolute configuration were elucidated by a combination of MS, NMR, and ECD calculation. The in vitro cytotoxic activities of 1 and 2...

7-Hydroxy questin, a new anthraquinone from a wetland fungus Aspergillus flavipes PJ03-11

Chong Yu, Shuang Lin, Xiaorui Ma, Jie Zhang, Caixia Wu, Ben Ma, Haifeng Wang & Yuehu Pei
One new anthraquinone, questinlin (1), four known anthraquinones (2–5), together with three known benzophenone derivatives (6–8) were isolated from a wetland fungus Aspergillus flavipes PJ03-11. Their structures were elucidated on the basis of detailed spectroscopic analysis, including 1D, 2D NMR (HMQC, HMBC), mass spectrometry, and optical rotation and IR spectra. In addition, the cytotoxic activities against three human cancer cell lines (HepG2, Caco-2 and A549) were evaluated. In this article, Aspergillus flavipes PJ03-11, a wetland...

Alkaloids from endophytic fungus Aspergillus fumigatus HQD24 isolated from the Chinese mangrove plant Rhizophora mucronata

Renjian Zou, Chengwen Wei, Xuexia Zhang, Dongdong Zhou & Jing Xu
Chemical investigation of endophytic fungus Aspergillus fumigatus HQD24, isolated from the flower of Rhizophora mucronata led to the isolation of eight alkaloids, including pyripyropene A (1), 1,11-dideacetyl-pyripyropene A (2), pyripyropene E (3), chaetominine (4), tryptoquivaline J (5), fumitremorgin C (6), 1-acetyl-β-carboline (7), and nicotinic acid (8). Their structures were unambiguously elucidated on the basis of extensive spectroscopic data and comparison with the data of literature. Compound 2 was known as a synthetic product and isolated...

Antibacterial activities of the chemical constituents of Schizophyllum commune MST7-3 collected from coal area

Guang-Gui Chen, Qin-Feng Zhu, Xing-Mei Long, Qian Lu, Kai-Yu Li, Qian Chen, Meng Zhou, Shang-Gao Liao & Guo-Bo Xu
Two fusidane-type active compounds (6 and 7) and five new ones (1–5), along with other nine known compounds (8–16) were isolated from the metabolites of Schizophyllum commune MST7-3. Their structures were elucidated on the basis of spectroscopic analysis. The absolute configurations of compounds 2 and 3 were established by Mosher’s method and optical rotation. Compounds 6 and 7 showed significant antibacterial activities against Stenotrophomonas maltophilia with MIC values of 4 μg/mL and 16 μg/mL, respectively.

Long intergenic non-protein coding RNA 02570 promotes nasopharyngeal carcinoma progression by adsorbing microRNA miR-4649-3p thereby upregulating both sterol regulatory element binding protein 1, and fatty acid synthase

Fei Liu, Jiazhang Wei, Yanrong Hao, Jiao Lan, Wei Li, Jingjin Weng, Min Li, Cheng Su, Bing Li, Mingzheng Mo, Fengzhu Tang, Yongli Wang, Yong Yang, Wei Jiao & Shenhong Qu
Our previous studies have elucidated a possible connection between long intergenic non-protein coding RNA 2570 (LINC02570) and nasopharyngeal carcinoma (NPC). However, the precise mechanism by which LINC02570 promotes NPC remains unknown. We used quantitative polymerase chain reaction (qPCR) to detect LINC02570 expression in nasopharyngeal cell lines, NPC tissues, and chronic rhinitis tissues. Subcellular LINC02570 localization was confirmed by fluorescence in situ hybridization (FISH). The effects of LINC02570 stable knockdown and overexpression on viabillity, proliferation, migration,...

Hypoxia promotes the proliferation of mouse liver sinusoidal endothelial cells: miRNA–mRNA expression analysis

Zhe Qing, Hanfei Huang, Qun Luo, Jie Lin, Shikun Yang, Tao Liu, Zhong Zeng & Tingfeng Ming
During the initial stage of liver regeneration (LR), hepatocytes and liver sinusoidal endothelial cells (LSECs) initiate regeneration in a hypoxic environment. However, the role of LSECs in liver regeneration in hypoxic environments and their specific molecular mechanism is unknown. Therefore, this study aimed to explore the miRNA–mRNA network that regulates the proliferation of LSECs during hypoxia. In this study, first, we found that the proliferation ability of primary LSECs treated with hypoxia was enhanced compared...

Ferritinophagy is involved in the zinc oxide nanoparticles-induced ferroptosis of vascular endothelial cells

Xia Qin, Jun Zhang, Bin Wang, Ge Xu, Xi Yang, Zhen Zou & Chao Yu
Zinc oxide nanoparticles (ZnONPs) hold great promise for biomedical applications. Previous studies have revealed that ZnONPs exposure can induce toxicity in endothelial cells, but the underlying mechanisms have not been fully elucidated. In this study, we report that ZnONPs can induce ferroptosis of both HUVECs and EA.hy926 cells, as evidenced by the elevation of intracellular iron levels, lipid peroxidation and cell death in a dose- and time-dependent manner. In addition, both the lipid reactive oxygen...

Folate-functionalized SMMC-7721 liver cancer cell membrane-cloaked paclitaxel nanocrystals for targeted chemotherapy of hepatoma

Wenwen Shen, Shuke Ge, Xiaoyao Liu, Qi Yu, Xue Jiang, Qian Wu, YuChen Tian, Yu Gao, Ying Liu & Chao Wu
In this study, we prepared a folic acid-functionalized SMMC-7721 liver cancer cell membrane (CM)-encapsulated paclitaxel nanocrystals system (FCPN) for hepatoma treatment. Transmission electron microscopy (TEM) characterization showed that FCPN was irregular spherical shapes with a particle size larger than 200 nm and a coated thickness of approximately 20 nm. In an in vitro release experiment, FCPN indicated a slowly release effect of paclitaxel (PTX). Cell experiments demonstrated that FCPN was taken up by SMMC-7721 cells...

A new cyclopentenone derivative from Trichoderma atroviride HH-01

Li Zhu, Jia-Peng Wang, Feng Hao, Dong Gan, Xiao-Ran Zhang, Chen-Zhe Li, Cheng-Yao Wang, Liang Zhang & Le Cai
A new cyclopentenone derivative, atrovinol (1), together with ten known compounds (2-11) were isolated from Trichoderma atroviride HH-01, an endophytic fungus from Illigera rhodantha (Hernandiaceae). Their structures were identified by HRESIMS, 1 D/2D NMR, and electronic circular dichroism (ECD) spectra. Compound 1 exhibited moderate inhibitory activity against Staphylococcus aureus and Bacillus subtilis with MIC values of 8.0 µg/mL and 16.0 µg/mL, respectively.

Long non-coding RNA RP11-283G6.5 confines breast cancer development through modulating miR-188-3p/TMED3/Wnt/β-catenin signaling

Jing Pei, Shengquan Zhang, Xiaowei Yang, Chunguang Han, Yubo Pan, Jun Li, Zhaorui Wang, Chenyu Sun & Jing Zhang
The contributions of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) to breast cancer are critical areas of investigation. In this study, we identified a novel lncRNA RP11-283G6.5 which was lowly expressed in breast cancer and whose low expression was correlated with poor overall survival and disease-free survival of breast cancer patients. Functional experiments revealed that ectopic expression of RP11-283G6.5 confined breast cancer cellular growth, migration, and invasion, and promoted cellular apoptosis. Conversely, RP11-283G6.5 silencing facilitated...

Perchlorate bioreduction in UASB reactor: S2--autotrophic granular sludge formation and sulphate generation control

Rui Zhao, Huaqiang Tao, Yuanyuan Song, Jianbo Guo, Zhi Chen, Caicai Lu, Yi Han, Haibo Li & Yanan Hou
Perchlorate (ClO4−) industrial wastewater requires efficient removal to prevent adverse environmental impacts, however, high concentration and low biodegradability give rise to poor ClO4− bioreduction performance. S2−-autotrophic granular sludge (S2−-AuGS) was firstly cultivated for high concentration perchlorate (ClO4−) removal in the upflow anaerobic sludge blanket (UASB) reactor (ClO4−: 150 mg L−1). Simultaneously, the S2− was utilized to control the SO42− generation as electron donor, the effluent SO42− concentration (190 mg L−1) was satisfied with drinking water...

Optimization of the extraction conditions and dermal toxicity of oil body fused with acidic fibroblast growth factor (OLAF)

Yongxin Guo, Yaying Li, Qian Wu, Xinxin Lan, Guodong Chu, Weidong Qiang, Muhammad Noman, Tingting Gao, Jinnan Guo, Long Han, Jing Yang, Xiaokun Li & Linna Du
Oil body (OB), a subcellular organelle that stores oil in plant seeds, is considered a new transdermal drug delivery system. With the increasing understanding of the OB and its main protein (oleosin), numerous studies have been conducted on OB as “carrier” for the expression of exogenous proteins. In our previous study, oil body fused with aFGF (OLAF) was obtained using a plant oil body expression system that had been preliminarily proven to be effective in...

Optimization of the extraction conditions and dermal toxicity of oil body fused with acidic fibroblast growth factor (OLAF)

Yongxin Guo, Yaying Li, Qian Wu, Xinxin Lan, Guodong Chu, Weidong Qiang, Muhammad Noman, Tingting Gao, Jinnan Guo, Long Han, Jing Yang, Xiaokun Li & Linna Du
Oil body (OB), a subcellular organelle that stores oil in plant seeds, is considered a new transdermal drug delivery system. With the increasing understanding of the OB and its main protein (oleosin), numerous studies have been conducted on OB as “carrier” for the expression of exogenous proteins. In our previous study, oil body fused with aFGF (OLAF) was obtained using a plant oil body expression system that had been preliminarily proven to be effective in...

Nickel (ii) effects on Anammox reaction: reactor performance, dehydrogenase, sludge morphology and microbial community changes

Jian Cheng, Liang Qiao, Wen Xu, Yulan Qian, Yiyang Ge, Ting Xia & Yan Li
Nickel (ii) (Ni2+) is considered as one of the necessary trace elements in the process of Anammox culture, but it may have toxic effects at high concentration. This study explored the long-term influence of Ni2+ on the denitrification efficiency of Anammox bioreactors. The results showed that when the concentration of Ni2+ was 0.5 mg/L, the bioreactor had the highest denitrification efficiency, while the removal efficiency of ammonia nitrogen and nitrite nitrogen gradually decreased at concentrations...

Sex difference in lipid levels in first-diagnosed drug-naïve depression patients: A case-control and 12-weeks follow-up study

Rui Yang, Lu Wang, Song Cao, Ming Chen, Chu-Jun Wu, Floyd Silva, Man-Jun Shen, Jin-Dong Chen, Mi-Mi Tang & Bi-Lian Liu
Patients with depression have a high prevalence of developing dyslipidemia. In this study, we aim to investigate the difference of serum lipids, including total cholesterol (TCH), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG), between the depressed patients and healthy controls. Sex differences in lipids and their psychological correlations were also included. The study included 56 healthy controls (males/females = 26/30) and 110 first-diagnosed drug-naïve outpatients (males/females = 35/75). A total of...

Biological mechanisms of growth performance and meat quality in porcine muscle tissue

Chen Fang, Fei Guo, Xiaoqi Zhao, Zining Zhang, Junlan Lu, Hongbin Pan, Taojie Xu, Weizhen Li, Minghua Yang, Ying Huang, Yanguang Zhao & Sumei Zhao
Growth performance and meat quality are important traits for pig production. The aim of the present study was to investigate the molecular mechanisms underlying growth performance and meat quality, and to identify novel target molecules for predicting the growth performance and meat quality. The differentially expressed genes (DEGs) in Diannan small ears pigs (DSP) and Landrace pigs (LP) were assessed by RNA-sequencing analyzing technology. A total of 339 DEGs were obtained between DSP and LP....

Registration Year

  • 2021

Resource Types

  • Text
  • Dataset
  • Image
  • Collection
  • Other
  • Audiovisual


  • Soochow University
  • Fudan University
  • Zhejiang University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Shanghai Jiao Tong University
  • Sun Yat-sen University
  • Capital Medical University
  • Huazhong University of Science and Technology
  • Sichuan University
  • Chinese Academy of Sciences