3,039 Works

Role of lncRNA LINC01194 in hepatocellular carcinoma via the miR-655-3p/SMAD family member 5 axis

Yang Liu, Jie Liu, Junkai Cui, Ruolei Zhong & Guoyang Sun
Long non-coding RNAs (lncRNAs) are involved in developing hepatocellular carcinoma (HCC). The present study explored the role of lncRNA LINC01194, which is upregulated in HCC tissues and might be a vital regulator in HCC progression. Levels of LINC01194, microRNA (miR)-655-3p, and SMAD family member 5 (SMAD5) were assessed using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The bioactivity of Huh-7 cells was assessed using cell counting kit-8 and transwell assays and flow cytometry. Western...

Long non-coding RNA terminal differentiation-induced non-coding RNA regulates cisplatin resistance of choroidal melanoma by positively modulating extracellular signal-regulated kinase 2 via sponging microRNA-19b-3p

Wei Li, Aiqin Nie, Longyu Jin, Yubo Cui, Ning Xie & Gaohua Liang
In the present study, we aimed to investigate the role of long non-coding RNA terminal differentiation-induced non-coding RNA (TINCR) in cisplatin (DDP) resistance of choroidal melanoma (CM) and the potential molecular mechanisms. CM and non-CM tissues were collected from 60 CM patients. DDP-resistant CM cells were obtained by selection with linearly increased DDP treatment. The expression levels of TINCR, microR-19b-3p (miR-19b-3p), and extracellular signal-regulated kinase 2 (ERK-2) were detected by quantitative real-time PCR. Cholecystokinin octapeptide...

Metabolomics research on the effect of dexamethasone‐induced chronic stress in serum of rabbits

Hongli Liu, Bin Zhang, Lei Liu & Fuchang Li
Stress is a common factor that reduces production performance. However, the physiology of stress is not fully understood. In this study, 40-day-old rabbits were injected 1.0 mg/kg BW Dexamethasone to induce stress. A metabolomic study on serum of stress-induced rabbits was performed to research the metabolism mechanism of stress by using ultra-performance liquid chromatography coupled to mass spectrometry (UPLC–MS). Clear separations between stress rabbits and control rabbits were observed by principal component analysis (PCA) based...

Metabolomics research on the effect of dexamethasone‐induced chronic stress in serum of rabbits

Hongli Liu, Bin Zhang, Lei Liu & Fuchang Li
Stress is a common factor that reduces production performance. However, the physiology of stress is not fully understood. In this study, 40-day-old rabbits were injected 1.0 mg/kg BW Dexamethasone to induce stress. A metabolomic study on serum of stress-induced rabbits was performed to research the metabolism mechanism of stress by using ultra-performance liquid chromatography coupled to mass spectrometry (UPLC–MS). Clear separations between stress rabbits and control rabbits were observed by principal component analysis (PCA) based...

Nutritional components and protein quality analysis of genetically modified phytase maize

Yichun Hu, Liqin Linghu, Min Li, Deqian Mao, Yu Zhang, Xiaoguang Yang & Lichen Yang
The nutritional components and protein quality of genetically modified maize expressing phytase gene (GM) were analyzed and evaluated in this study. The nutritional components were analyzed by Chinese national standard methods. The ileostomy Bama miniature pigs were utilized to analyze the true digestibility of protein and amino acids. The digestible indispensable amino acid score (DIAAS) was adopted to evaluate the protein quality of GM, its parental maize (PM) and commercial available maize Zhengdan 958 (ZD)....

Ultrasound targeted microbubble destruction-mediated SOCS3 attenuates biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells

Xiaojiang Tang, Na Hao, Yuhui Zhou & Yang Liu
SOCS3 is low-expressed in breast cancer and may be a potential target. Ultrasound targeted microbubble destruction (UTMD) improved the efficiency of gene transfection. We explored the effects of UTMD-mediated transfection of SOCS3 on the biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells (BCSCs). The expression of SOCS3 in breast cancer (BC) and its association with prognosis were evaluated by GEPIA and The Cancer Genome Atlas (TCGA) websites. BCSCs were sorted by flow...

Aetiology of severe community acquired pneumonia in adults identified by combined detection methods: a multi-centre prospective study in China

Jieming Qu, Jing Zhang, Yu Chen, Yi Huang, Yusang Xie, Min Zhou, Yuping Li, Dongwei Shi, Jinfu Xu, Qiuyue Wang, Bei He, Ning Shen, Bin Cao, Danyang She, Yi Shi, Xin Su, Hua Zhou, Hong Fan, Feng Ye, Qiao Zhang, Xinlun Tian & Guoxiang Lai
Severe Community Acquired Pneumonia (SCAP) challenges public health globally. Considerable improvements in molecular pathogen testing emerged in the last few years. Our prospective study combinedly used traditional culture, antigen tests, PCR and mNGS in SCAP pathogen identification with clinical outcomes. From June 2018 to December 2019, we conducted a multi-centre prospective study in 17 hospitals of SCAP patients within 48 hours of emergency room stay or hospitalization in China. All clinical data were uploaded into...

Aetiology of severe community acquired pneumonia in adults identified by combined detection methods: a multi-centre prospective study in China

Jieming Qu, Jing Zhang, Yu Chen, Yi Huang, Yusang Xie, Min Zhou, Yuping Li, Dongwei Shi, Jinfu Xu, Qiuyue Wang, Bei He, Ning Shen, Bin Cao, Danyang She, Yi Shi, Xin Su, Hua Zhou, Hong Fan, Feng Ye, Qiao Zhang, Xinlun Tian & Guoxiang Lai
Severe Community Acquired Pneumonia (SCAP) challenges public health globally. Considerable improvements in molecular pathogen testing emerged in the last few years. Our prospective study combinedly used traditional culture, antigen tests, PCR and mNGS in SCAP pathogen identification with clinical outcomes. From June 2018 to December 2019, we conducted a multi-centre prospective study in 17 hospitals of SCAP patients within 48 hours of emergency room stay or hospitalization in China. All clinical data were uploaded into...

Morphology and phylogeny of two new Thalassiosiroid taxa (Bacillariophyceae), with two marginal rimoportulae

Biaobiao Niu, Yaqiong Guo, Nina Lundholm & Yang Li
Thalassiosira and related taxa i.e. the taxa within Thalassiosirales are among the most abundant and widespread diatoms. The vast majority have one marginal rimoportula, but very few always have two. The knowledge on Thalassiosiroid taxa in Chinese waters is restricted, and to explore the diversity, monoclonal strains were established from southern Chinese coastal waters. Morphological data were based on light and scanning electron microscopy. The D1‒D3 region of the ribosomal large subunit (LSU) and the...

Short divalent ethacrynic amides as pro-inhibitors of glutathione S-transferase isozyme Mu and potent sensitisers of cisplatin-resistant ovarian cancers

Bangtian Xu, Tingting Tong, Xin Wang, Fang Liu, Xiang Zhang, Xiaolei Hu, Xinpeng Li, Xiaolan Yang & Fei Liao
The linking of ethacrynic acid with ethylenediamine and 1,4-butanediamine gave EDEA and BDEA, respectively, as membrane-permeable divalent pro-inhibitors of glutathione S-transferase (GST). Their divalent glutathione conjugates showed subnanomolar inhibition and divalence-binding to GSTmu (GSTM) (PDB: 5HWL) at ∼0.35 min−1. In cisplatin-resistant SK-OV-3, COC1, SGC7901 and A549 cells, GSTM activities probed by 15 nM BDEA or EDEA revealed 5-fold and 1.0-fold increases in cisplatin-resistant SK-OV-3 and COC1 cells, respectively, in comparison with the susceptible parental cells....

Short divalent ethacrynic amides as pro-inhibitors of glutathione S-transferase isozyme Mu and potent sensitisers of cisplatin-resistant ovarian cancers

Bangtian Xu, Tingting Tong, Xin Wang, Fang Liu, Xiang Zhang, Xiaolei Hu, Xinpeng Li, Xiaolan Yang & Fei Liao
The linking of ethacrynic acid with ethylenediamine and 1,4-butanediamine gave EDEA and BDEA, respectively, as membrane-permeable divalent pro-inhibitors of glutathione S-transferase (GST). Their divalent glutathione conjugates showed subnanomolar inhibition and divalence-binding to GSTmu (GSTM) (PDB: 5HWL) at ∼0.35 min−1. In cisplatin-resistant SK-OV-3, COC1, SGC7901 and A549 cells, GSTM activities probed by 15 nM BDEA or EDEA revealed 5-fold and 1.0-fold increases in cisplatin-resistant SK-OV-3 and COC1 cells, respectively, in comparison with the susceptible parental cells....

Formation and characterization of Pt-Cu-IPMC with high specific surface area and dendritic electrode

Aifen Tian, Jiahua Li, Xixi Wang, Yue Sun, Xinrong Zhang, Shanshan Yao & Huiling Du
Ionic polymer–metal composite (IPMC) is a kind of electroactive intelligent material. The dendritically interfacial electrodes (DIEs) is very important to the performance of IPMC. Herein, the copper–platinum composite electrode (Pt-Cu-IPMC) with a high specific area DIEs was prepared by combining impregnation-reduction plating platinum (IRP-Pt), autocatalytic plating platinum (ACP-Pt) and chemical reduction plating copper (CRP-Cu). The effects of DIEs and the cycles of ACP-Pt have been systematically investigated on the electrode’s thickness, micro-morphology and electromechanical response...

Knockdown of circ_0001679 alleviates lipopolysaccharide-induced MLE-12 lung cell injury by regulating the miR-338-3p/ mitogen-activated protein kinase 1 axis

Shenggui Lu, Xinmiao Wu, Shuai Xin, Jing Zhang, Hanying Lin, Yu Miao & Yixin Li
The upregulation of circ_0001679 was reported in lipopolysaccharide (LPS)-induced lung injury mouse model, but its functional roles and mechanisms in LPS-induced lung injury remain to be investigated. In this study, we aimed to explore the potential role of circ_0001679 in septic acute lung injury. We initially established an in vitro lung cell injury model using LPS-treated MLE-12 cells. siRNAs targeting circRNA_0001679 were employed to stably knock down circRNA_0001679, followed by functional assays to investigate the...

Utilization of far-red LED to minimize blue light hazard for dynamic semiconductor lighting

Jingxin Nie, Zhizhong Chen, Fei Jiao, Yifan Chen, Jinglin Zhan, Yiyong Chen, Zuojian Pan, Xiangning Kang, Yongzhi Wang, Qi Wang, Weimin Dang, Wentian Dong, Shuzhe Zhou, Xin Yu, Yuzhen Tong, Guoyi Zhang & Bo Shen
The blue light hazard (BLH) and luminous efficacy of a source (ηV) are associated with the photobiological safety and energy saving of light-emitting diodes (LEDs), respectively. In this study, we used genetic algorithm (GA) to optimize the BLH, ηV, and color rendering parameters of five-chip hybrid white LEDs. Based on the optimal results, we used five-chip LEDs to obtain the hybrid white light. Their peak wavelengths were 461.5 (blue), 523.9 (green), 588.2 (orange), 643.3 (red),...

Utilization of far-red LED to minimize blue light hazard for dynamic semiconductor lighting

Jingxin Nie, Zhizhong Chen, Fei Jiao, Yifan Chen, Jinglin Zhan, Yiyong Chen, Zuojian Pan, Xiangning Kang, Yongzhi Wang, Qi Wang, Weimin Dang, Wentian Dong, Shuzhe Zhou, Xin Yu, Yuzhen Tong, Guoyi Zhang & Bo Shen
The blue light hazard (BLH) and luminous efficacy of a source (ηV) are associated with the photobiological safety and energy saving of light-emitting diodes (LEDs), respectively. In this study, we used genetic algorithm (GA) to optimize the BLH, ηV, and color rendering parameters of five-chip hybrid white LEDs. Based on the optimal results, we used five-chip LEDs to obtain the hybrid white light. Their peak wavelengths were 461.5 (blue), 523.9 (green), 588.2 (orange), 643.3 (red),...

Middle Permian magmatism in the Tangjia-Sumdo region, Tibet: evidence for intra-oceanic subduction

Bin Wang, Chao-Ming Xie, Carlos A. Spier, Yong-Sheng Dong, Yun-Peng Yu, Yu-Hang Song, Meng-Long Duan & Chris Yakymchuk
The late Paleozoic tectonic evolution of the Lhasa terrane and subduction of Sumdo Paleo-Tethys ocean remains controversial due to limited data and variable Permian magmatism in central and southern Lhasa. We report new zircon U–Pb ages and Hf-isotope ratios as well as whole-rock major element, trace element, and Sr–Nd isotope data from middle Permian (266 ~ 257 Ma) arc-related magmatism, including high-Mg quartz diorite and trondhjemite in the Tangjia-Sumdo area. The quartz diorite has high...

Abietane diterpenoids with neuroprotective activities from Phlegmariurus carinatus

Yang Liu, Qiang Wang, Dong-Kun Zheng, De Zhang, Zhen Xie, Jia-Wei Hu, Xin-Hua Xie, Jing Li & Shu-Ping Jiang
Two new abietane diterpenoids, phlecarinatone A (1) and phlecarinatone B (2), along with two known analogues (3 and 4), were isolated from Phlegmariurus carinatus. The structures of 1 − 4 were unambiguously elucidated by comprehensive spectroscopic analyses. Abietane diterpenoids were isolated from the plant for the first time. All isolates were tested for their neuroprotective activities against H2O2-induced SH-SY5Y cells injury, and compound 2 showed moderate effect at the concentrations ranging from 5 ∼ 20...

Two QTL for kernel number per spike identified from durum wheat

Jingtian Yang, Zhengqiao Liao, Baoguo Du, Kuan Zhang & Lei Liu
As one of the three yield components, kernel number per spike (KNPS) plays a key role in determining wheat yield. In this study, in combination with the previously constructed genetic map for a tetraploid wheat recombinant inbred line (RIL) population developed from the cross between a durum wheat and a wild emmer wheat and phenotype data from four environments, two major and stably expressed quantitative trait loci (QTL) for KNPS were identified. The phenotypic variation...

FURIN promoter methylation predicts the risk of incident diabetes: A prospective analysis in the Gusu cohort

Hao Peng
Background: Furin has been associated with diabetes but the underlying mechanisms are unclear. As a mediator linking fixed genome and dynamic environment, DNA methylation of its coding gene FURIN may be involved. Here, we aimed to examine the prospective association between DNA methylation in FURIN promoter and incident diabetes during 4 years of follow-up in Chinese adults. Methods: DNA methylation levels in FURIN promoter were quantified by target bisulfite sequencing using peripheral blood from 1836...

Upstream open reading frames mediate autophagy-related protein translation

Ying Yang, Damián Gatica, Xu Liu, Runliu Wu, Rui Kang, Daolin Tang & Daniel J. Klionsky
Macroautophagy/autophagy, a highly conserved catabolic pathway that maintains proper cellular homeostasis is stringently regulated by numerous autophagy-related (Atg) proteins. Many studies have investigated autophagy regulation at the transcriptional level; however, relatively little is known about translational control. Here, we report the upstream open reading frame (uORF)-mediated translational control of multiple Atg proteins in Saccharomyces cerevisiae and in human cells. The translation of several essential autophagy regulators in yeast, including Atg13, is suppressed by canonical uORFs...

Transcriptome analysis of thymic tissues from Chinese Partridge Shank chickens with or without Newcastle disease virus LaSota vaccine injection via high-throughput RNA sequencing

Furong Nie, Jingfeng Zhang, Mengyun Li, Xuanniu Chang, Haitao Duan, Haoyan Li, Jia Zhou, Yudan Ji & Liangxing Guo
The LaSota strain of Newcastle disease virus (NDV) is a commonly used vaccine to control Newcastle disease. However, improper immunization is a common reason for vaccine failure. Hence, it is imperative to thoroughly explore innate immunity-related molecular regulatory responses to the LaSota vaccine. In this text, 140 long non-coding RNAs (lncRNAs), 8 microRNAs (miRNAs), and 1514 mRNAs were identified to be differentially expressed by RNA sequencing analysis in the thymic tissues of Chinese Partridge Shank...

Hepatoprotective mechanism of Silybum marianum on nonalcoholic fatty liver disease based on network pharmacology and experimental verification

Guoyan Jiang, Chunhong Sun, Xiaodong Wang, Jie Mei, Chen Li, Honghong Zhan, Yixuan Liao, Yongjun Zhu & Jingxin Mao
The study aimed to identify the key active components in Silybum marianum (S. marianum) and determine how they protect against nonalcoholic fatty liver disease (NAFLD). TCMSP, DisGeNET, UniProt databases, and Venny 2.1 software were used to identify 11 primary active components, 92 candidate gene targets, and 30 core hepatoprotective gene targets in this investigation, respectively. The PPI network was built using a string database and Cytoscape 3.7.2. The KEGG pathway and GO biological process enrichment,...

Identification of AhATL1 interaction proteins participating in drought stress memory in peanut

Xinquan Wu, Xiaoyan Li, Yinglin Huang & Bo Hu
Plants have evolved an adaptive mechanism named drought stress memory to improve resistance and increase survival chances, by means of coordination of physiological responses and changes of gene expression patterns. The ATP-binding cassette (ABC) superfamily G proteins are membrane proteins that are involved in abscisic acid (ABA) transport and the response to drought stress. The ABC protein AhATL1 could play an important role in drought resistance by affecting ABA importation and stomatal closure and could...

A novel genetic variant potentially altering the expression of MANBA in the cerebellum associated with attention deficit hyperactivity disorder in Han Chinese children

Xinzhen Chen, Ting Yao, Jinliang Cai, Qi Zhang, Shanyawen Li, Huiru Li, Xihang Fu & Jing Wu
To obtain additional insight into the genetic factors of attention deficit hyperactivity disorder (ADHD). First, we performed a transcriptome-wide association study (TWAS) integrating human cerebellum-specific variant-expression/splicing correlations to identify ADHD susceptibility genes. Then, the associations between expression/splicing quantitative trait loci (eQTLs/sQTLs) of the transcriptome-wide significant genes and ADHD were observed in a case-control study of Han Chinese children. Furthermore, dual luciferase reporter gene assays were performed to validate the regulatory function of ADHD risk variants....

The response surface optimization of β-mannanase produced by Weissella cibaria F1 and its potential in juice clarification

Hairui Ji, Huiying Cao, Li Zhao, Ruiying Na, Wenxiang Ping, Jingping Ge & Dan Zhao
A β-mannanase-producing lactic acid bacteria (LAB) was identified as Weissella cibaria F1 according to physiological and biochemical properties, morphological observations, partial sequence of 16S rRNA gene and API 50 CHL test. In order to improve the yield of β-mannanase, the response surface methodology (RSM) was originally used to optimize the fermentation conditions. The optimization results showed that when the konjac powder, glucose, and initial pH were 9.46 g/L, 14.47 g/L and 5.67, respectively, the β-mannanase...

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