Additional file 1: Corresponding uncropped full-length gels and blot in the supplementary file.

Abstract Background and aims Human umbilical cord mesenchymal stem cells (hUC-MSCs) are increasingly being studied in clinical trials of end-stage liver disease because of their good tissue repair and anti-inflammatory effects. hUC-MSC exosomes are vesicles with spherical structures secreted by cells that produce them. The diameter of exosomes is much smaller than that of hUC-MSCs, suggesting that exosomes might be a novel and safer therapeutic product of mesenchymal stem cells. As exosomes have been suggested...

High-resolution figures for Fig. 4 (a)-(d), Fig. 5 (b)(e)(h), Fig.11 (a) (b) and Fig.14 (a)(b)(d)(e), respectively.

In this work, we proposed an optical trapping and manipulation technology based on spatial diffraction of 45° tilted fiber Bragg grating (TFBG). The length of the line-shape-facula of the TFBG diffraction light can be as large as tens of millimeters, which enables the TFBG trapping system control massive dielectric particles. We analyze the light distribution of the spatial diffraction by using the volume current method (VCM) and established a theoretical model to analyze the optical...

supporting information

Silicon bowtie structure based all-nonmetal metamaterial (ANM) terahertz device has been developed, which has comparable efficiency to that of their metallic counterparts, and better compatibility with modern semiconductor fabrication processes. Moreover, with the same structure but highly tunable ANM is successfully fabricated through integrating with a flexible substrate, it demonstrates large tunability over a wide frequency range. Such a device can be used in terahertz systems for numerous applications, and potentially be a promising substitute...

Additional file 4: Fig. S3 Effect of adipocyte CM on the expression level of Snail, Slug, ZEB1 and ZEB2 in EOC cells. A SKOV3 cells were serum starved overnight and cultured in SFM or adipocyte CM for 48 h. Expression levels of Snail, Slug, ZEB1 and ZEB2 were determined by Western blot and normalized to GAPDH. Densitometric analyses were shown on the right. Experiments have been carried out with adipocytes from 2 to 3 independent...

Additional file 5: Fig. S4 Inhibition of Twist1 by siRNA suppressed adipocyte-induced metastasis of EOC cells. A SKOV3 and HEY cells were transfected with negative control siRNA (si-Ctrl) or Twist1 siRNA (si-Twist1). 24 h after transfection, mRNA level of Twist1 was determined by qRT-PCR and normalized to GAPDH. Data are mean ± SD (n = 3). **P < 0.01; two-tailed Student’s t-test. B 48 h after transfection, protein level of Twist1 was determined by Western...

Additional file 6: Fig. S5 Inhibition of SphK1 suppressed omental metastasis of EOC in vivo. A Expression of SphK1 in stable transfected SKOV3 cells tested by qRT-PCR and Western blot. GAPDH was used as a loading control. Data are mean ± SD (n = 3). *P < 0.05 versus Blank; ns, no significance versus Blank; two-tailed Student’s t-test. B Representative images of disseminated tumors in intraperitoneal EOC xenograft mouse models injected with Ctrl shRNA (sh-Ctrl)...

Chemotherapy is an important treatment modality for osteosarcoma (OS), but the development of chemoresistance limits the therapeutic efficacy of OS and results in a poor prognosis. Thus, a better understanding of the mechanisms underlying chemoresistance in OS is essential. We previously demonstrated that COPS3/CSN3 (COP9 signalosome subunit 3) functions as an oncogene to promote OS cells lung metastasis, which is closely related to chemoresistance. Here, we showed that COPS3 was significantly upregulated in OS tissues...

Chemotherapy is an important treatment modality for osteosarcoma (OS), but the development of chemoresistance limits the therapeutic efficacy of OS and results in a poor prognosis. Thus, a better understanding of the mechanisms underlying chemoresistance in OS is essential. We previously demonstrated that COPS3/CSN3 (COP9 signalosome subunit 3) functions as an oncogene to promote OS cells lung metastasis, which is closely related to chemoresistance. Here, we showed that COPS3 was significantly upregulated in OS tissues...

Additional file 2. The codes of this article.

Abstract Background Dandelion is an herb with high nutritional and medicinal values, which has been listed in Chinese Pharmacopeia, European Pharmacopoeia and British Pharmacopoeia, gaining increasing acceptance around the world. However, the current quality control of dandelion is lagging behind. Only in Chinese Pharmacopeia, cichoric acid is used as a marker compound for its quality evaluation, whereas, it can not comprehensively reflect the bioactivity of dandelion. Methods This study developed a strategy by integrating chemometrics...

Supplemental material, sj-pdf-2-jva-10.1177_11297298221139640 for Risk factors for in-stent restenosis in maintenance hemodialysis patients with central venous occlusive disease and biomechanical assessment of stents by Yang Yu, Yuqin Xiong, Tao Li, Jingyuan Zhou, Weijie Yan, Yan Xiong, Yu Chen and Ping Fu in The Journal of Vascular Access

Supplemental material, sj-pdf-3-jva-10.1177_11297298221139640 for Risk factors for in-stent restenosis in maintenance hemodialysis patients with central venous occlusive disease and biomechanical assessment of stents by Yang Yu, Yuqin Xiong, Tao Li, Jingyuan Zhou, Weijie Yan, Yan Xiong, Yu Chen and Ping Fu in The Journal of Vascular Access

Objectives:To investigate the risk factors and biomechanical mechanisms of in-stent restenosis (ISR) in central venous occlusive disease (CVOD).Patients and methods:This retrospective study consecutively included 77 maintenance hemodialysis (MHD) patients with CVOD who received the first percutaneous transluminal angioplasty with stenting (PTS) due to symptomatic CVOD in a tertiary hospital. The mean age was 59.7 ± 14.0 years, and 51.9% of patients were male. The clinical characteristics, occurrence of ISR and patency rates were recorded. Finite...

The newly identified earliest part of the Sanligang bimodal volcanic succession in the Dahongshan area of the northern Yangtze Block provides insight into the role of this block in the evolution of the Rodinia supercontinent. Field contact relationships and dating indicate that these rocks are contemporaneous and formed at 840–820 Ma. The Sanligang mafic rocks have low SiO2 (40.33–48.17 wt.%) and K2O (0.07–1.65 wt.%), high TiO2 (2.64–3.52 wt.%) contents, and low U/Th (0.33–0.38), Th/Zr (0.001–0.002),...

Heart failure (HF) remains a major cause of mortality, hospitalisations, and poor quality of life. The mortality and readmission rate of HF patients after discharge are still high due to poor self-care and adherence, or inability to detect signs of deterioration. Telehealth programs may benefit a broad range of patients and reduce the suffering from HF. We aimed to investigate the impact of a 12-week telehealth program on outcomes among discharged patients with HF. Study...

Additional file 2. PRECIS-2 scores of chaiqin chengqi decoction for predicted moderately severe and severe acute pancreatitis (CAP trial) domains.

With the development of microfluidics in recent years, micro/nano-scale bubbles have been considered an emerging tool and become a field of growing interest for addressing many challenges in various Lab on a Chip (LOC) applications. Researchers found that when acoustic fields interact with bubbles in microfluidics, it will cause various interesting physical phenomena, which offers promising abilities in the manipulation of cells, fluids and particles and provides effective ways for biological and biomedical applications. The...

Abstract Background MYCN amplification (MNA) has been proved to be related to poor prognosis in neuroblastoma (NBL), but the MYCN-related immune signatures and genes remain unclear. Methods Enrichment analysis was used to identify the significant enrichment pathways of differentially expressed immune-related genes (DEIRGs). Weight gene coexpression network analysis (WGCNA) was applied to reveal the correlation between these DEIRGs and MYCN status. Univariate and multivariate Cox analyses were used to construct risk model. The relevant fractions...

Here we proposed a polarization-dependent gradient phase modulation strategy and fabricated a local polarization matched metasurface to add/drop polarization multiplexed cylindrical vector beams (CVBs). The two orthogonal linear polarization states in CVB multiplexing will represent as radial- and azimuthal-polarized CVBs, which means that we must introduce independent wave vectors to them for adding/dropping the polarization channels. By designing the rotation angle and geometric sizes of meta-atom, a local polarization matched propagation phase plasmonic metasurface was...

Here we proposed a polarization-dependent gradient phase modulation strategy and fabricated a local polarization matched metasurface to add/drop polarization multiplexed cylindrical vector beams (CVBs). The two orthogonal linear polarization states in CVB multiplexing will represent as radial- and azimuthal-polarized CVBs, which means that we must introduce independent wave vectors to them for adding/dropping the polarization channels. By designing the rotation angle and geometric sizes of meta-atom, a local polarization matched propagation phase plasmonic metasurface was...

Background:The medial patellofemoral ligament (MPFL) is the most important stabilizer of lateral dislocation of the patella. Single-limb (SL) and double-limb (DL) graft MPFL reconstructions have been increasingly used to treat recurrent patellar dislocations, but the clinical efficacy of SL versus DL graft MPFL reconstructions remains controversial owing to the inconsistent conclusions of previous studies.Purpose:This study aimed to compare the clinical outcomes of SL and DL graft MPFL reconstructions for patients with recurrent patellar dislocation by...

Time evolution characteristics of spectrum and temperature of lightning discharge plasma: supplemental document