Introduction: Prompted by recent amendments of Yellow Fever (YF) vaccination guidelines from boost to single vaccination strategy and the paucity of clinical data to support this adjustment, we used the profile of the YF-specific CD8+ T-cell subset profiles after primary vaccination and neutralizing antibodies as a proxy for potentially longer lasting immunity. Methods and Findings: PBMCs and serum were collected in six individuals on days 0, 3, 5, 12, 28 and 180, and in 99...

Objectives: To develop, demonstrate and evaluate an automated deep learning method for multiple cardiovascular structure segmentation. Background: Segmentation of cardiovascular images is resource-intensive. We design an automated deep learning method for the segmentation of multiple structures from Coronary Computed Tomography Angiography (CCTA) images. Methods: Images from a multicenter registry of patients that underwent clinically-indicated CCTA were used. The proximal ascending and descending aorta (PAA, DA), superior and inferior vena cavae (SVC, IVC), pulmonary artery (PA),...

Abstract Background For mechanically ventilated critically ill COVID-19 patients, prone positioning has quickly become an important treatment strategy, however, prone positioning is labor intensive and comes with potential adverse effects. Therefore, identifying which critically ill intubated COVID-19 patients will benefit may help allocate labor resources. Methods From the multi-center Dutch Data Warehouse of COVID-19 ICU patients from 25 hospitals, we selected all 3619 episodes of prone positioning in 1142 invasively mechanically ventilated patients. We excluded...

Extraction protocol for the research study 'Low awareness of the transitivity assumption in complex networks of interventions: empirical evidence from 356 network meta-analyses'.

Additional file 2: Supplementary figure 2. BNRF1 is undetectable in SF antigen-specific ACPA-expressing B cells. Left: B2M copies in DNA of SF IgG memory B cells and ACPA-expressing B cells as detected by qPCR. Right: no detection of BNRF1 by qPCR in DNA of SF IgG memory B cells, ACPA-expressing B cells and CD40L feeder cells only. Each dot represents a pool of 20 cells isolated directly into lysis buffer, from one donor. qPCR experiments...

Abstract Objective Rheumatoid arthritis (RA) is characterized by the presence of disease-specific autoreactive B cell responses, in particular those generating anti-citrullinated protein antibodies (ACPA). For many years, Epstein-Barr virus (EBV) has been implicated in disease pathogenesis, possibly by facilitating the development and persistence of autoreactive B cells. To test this hypothesis, the presence of EBV episomes in ACPA-expressing B cells was analyzed. Methods ACPA-expressing B cells derived from peripheral blood (PB) of seven EBV-seropositive RA...

Additional file 3: Propagation walkscores of 127 proxy protein-coding genes in the TCGA-BRCA cohort.

Additional file 4: Canonical pathways, diseases and functions associated with the 127 proxy protein-coding genes.

Additional file 2: Scores on the explanatory variables in lower, middle and higher SES-groups.

Additional file 6: Prognostic value of ICR classifier across solid cancers. Forest plot showing HRs for death (overall survival) and corresponding 95%-confidence interval for the continuous ICR score and number of patients for each TCGA cancer cohort and RAQA breast cancer cohort. Significant positive HRs are visualized in blue and significant negative HRs are visualized in red. ICR enabled (HR < 1, p-value < 0.05) cancer types are indicated with orange asterisks and ICR disabled...

Abstract Background Advances in our understanding of the tumor microenvironment have radically changed the cancer field, highlighting the emerging need for biomarkers of an active, favorable tumor immune phenotype to aid treatment stratification and clinical prognostication. Numerous immune-related gene signatures have been defined; however, their prognostic value is often limited to one or few cancer types. Moreover, the area of non-coding RNA as biomarkers remains largely unexplored although their number and biological roles are rapidly...

Additional file 7: Akaike information criterion (AIC), delta AIC, and HRs for death (overall survival) and corresponding 95%-confidence interval for ICR and 3 ir-lncRNA signature in 18 solid cancer TCGA datasets and RAQA cohort.

Additional file 2.

Additional file 2.

Additional file 1. Supplementary Methods.

Additional file 2: Supplementary Table 1. Comparison of beta diversity by pairwise PERMANOVA between the sarcoidosis patients and controls for fungal and bacterial taxa sampled by different methods* and assessed by different beta diversity metrices. Supplementary Table 2. Spearman's correlation coefficients (rho) and p-values for differences in alpha diversity correlated with antimicrobial peptides.

Abstract Background The role of the pulmonary microbiome in sarcoidosis is unknown. The objectives of this study were the following: (1) examine whether the pulmonary fungal and bacterial microbiota differed in patients with sarcoidosis compared with controls; (2) examine whether there was an association between the microbiota and levels of the antimicrobial peptides (AMPs) in protected bronchoalveolar lavage (PBAL). Methods Thirty-five sarcoidosis patients and 35 healthy controls underwent bronchoscopy and were sampled with oral wash...

Additional file 3. Examples of internal conflicts in ADHD psychoeducation.

Additional file 1.

Additional file 2.

Additional file 3.

Additional file 5.

Additional file 2: Fig. S2. Jitter and hist plots (triple therapy-based PSM).

Additional file 3: Table. S1. Summary of statin plus evolocumab therapy-based PSM.

Additional file 2.