1,257,673 Works

Diagnostics of organizational structure efficiency of manufacturing enterprise management and liability for labour law violation

Ruslana Kramar & Ruslan Skrynkovskyy
The article reveals the essence of diagnostics of organizational structure efficiency of manufacturing enterprise management. It is been determined that the key business indices of the diagnostics system of organizational structure efficiency of manufacturing enterprise management are: the efficiency level of the managerial apparatus activity; the performance level of the enterprise managerial apparatus; the level of provision of an enterprise management organizational structure with necessary resources; the level of responsibility of the management organizational structure...

CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Abstract Background Genome editing tools are important for functional genomics research and biotechnology applications. Recently, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 (Cas9) system for gene knockout has emerged as the most effective genome-editing tool. It has previously been reported that, in rice plants, knockdown of the Os8N3 gene resulted in enhanced resistance to Xanthomonas oryzae pv. oryzae (Xoo), while displaying abnormal pollen development. Results The CRISPR/Cas9 system was employed to knockout rice...

CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Abstract Background Genome editing tools are important for functional genomics research and biotechnology applications. Recently, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 (Cas9) system for gene knockout has emerged as the most effective genome-editing tool. It has previously been reported that, in rice plants, knockdown of the Os8N3 gene resulted in enhanced resistance to Xanthomonas oryzae pv. oryzae (Xoo), while displaying abnormal pollen development. Results The CRISPR/Cas9 system was employed to knockout rice...

Additional file 6 of CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Figure S6. Gross morphology of Kitaake and two homozygous Os8N3 mutant lines, T3 progeny of 3A-6-1 and 4A-1-7. (PDF 72 kb)

Additional file 7: of CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Figure S7. Seed-setting rates of homozygous xa13 mutants. a Representative panicles from Kitaake, homozygous mutant (T3, 3A-6-1-2), and homozygous mutant (T3, 4A-1-7-4). b Seed-setting rates of Kitaake, homozygous mutant (progeny of 3A-6-1), and homozygous mutant (progeny of 4A-1-7). c Mature caryopses of Kitaake, homozygous mutant (T3, 3A-6-1-1), and homozygous mutant (T3, 4A-1-7-1). Scale bars, 2.5â mm. (PDF 78 kb)

Additional file 6 of CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Figure S6. Gross morphology of Kitaake and two homozygous Os8N3 mutant lines, T3 progeny of 3A-6-1 and 4A-1-7. (PDF 72 kb)

Additional file 7: of CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Figure S7. Seed-setting rates of homozygous xa13 mutants. a Representative panicles from Kitaake, homozygous mutant (T3, 3A-6-1-2), and homozygous mutant (T3, 4A-1-7-4). b Seed-setting rates of Kitaake, homozygous mutant (progeny of 3A-6-1), and homozygous mutant (progeny of 4A-1-7). c Mature caryopses of Kitaake, homozygous mutant (T3, 3A-6-1-1), and homozygous mutant (T3, 4A-1-7-1). Scale bars, 2.5â mm. (PDF 78 kb)

Additional file 3: of CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Figure S3. Sequencing chromatogram at the target site of Os8N3 in the CRISPR/Cas9-induced plants (OsU6a xa13m/Kit T1). The vertical arrowhead indicates an expected cleavage site. (PDF 232 kb)

Additional file 5: of CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Figure S5. Sequencing chromatogram at the target site of Os8N3 in the CRISPR/Cas9-induced plants (OsU6a xa13m/Kit T3). The vertical arrowhead indicates an expected cleavage site. (PDF 196 kb)

Additional file 5: of CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Figure S5. Sequencing chromatogram at the target site of Os8N3 in the CRISPR/Cas9-induced plants (OsU6a xa13m/Kit T3). The vertical arrowhead indicates an expected cleavage site. (PDF 196 kb)

Additional file 1: of CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Figure S1. Sequence comparison of OsU6a promoters from Japonica cultivar Kitaake and Indica cultivar 93â 11. (PDF 72 kb)

Additional file 1: of CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Figure S1. Sequence comparison of OsU6a promoters from Japonica cultivar Kitaake and Indica cultivar 93â 11. (PDF 72 kb)

Additional file 2: of CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Figure S2. Sequencing chromatogram at the target site of Os8N3 in the CRISPR/Cas9-induced plants (OsU6a xa13m/Kit T0). The vertical arrowhead indicates an expected cleavage site. (PDF 105 kb)

Additional file 4: of CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Figure S4. Sequencing chromatogram at the target site of Os8N3 in the CRISPR/Cas9-induced plants (OsU6a xa13m/Kit T2). The vertical arrowhead indicates an expected cleavage site. (PDF 188 kb)

Additional file 4: of CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Figure S4. Sequencing chromatogram at the target site of Os8N3 in the CRISPR/Cas9-induced plants (OsU6a xa13m/Kit T2). The vertical arrowhead indicates an expected cleavage site. (PDF 188 kb)

Additional file 2: of CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Figure S2. Sequencing chromatogram at the target site of Os8N3 in the CRISPR/Cas9-induced plants (OsU6a xa13m/Kit T0). The vertical arrowhead indicates an expected cleavage site. (PDF 105 kb)

Additional file 3: of CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

Young-Ah Kim, Hyeran Moon & Chang-Jin Park
Figure S3. Sequencing chromatogram at the target site of Os8N3 in the CRISPR/Cas9-induced plants (OsU6a xa13m/Kit T1). The vertical arrowhead indicates an expected cleavage site. (PDF 232 kb)

Metabolomics for predicting hyperglycemia in pregnancy: a protocol for a systematic review and potential meta-analysis

Bianca Nicolosi, Debora Leite, Jussara Mayrink, Renato Souza, José Cecatti & Iracema Calderon
Abstract Background Hyperglycemia in pregnancy (HIP) has been recently differentiated between diabetes in pregnancy (DIP) and gestational diabetes mellitus (GDM). The proposed protocol is relevant, and clinical concern is due to the higher risk of adverse pregnancy outcomes (APO) and long-term effects on both the mother and the fetus. Fasting plasma glucose level (FPG) and oral glucose tolerance test (OGTT) are current diagnostic tools. However, controversy persists concerning diagnostic criteria, cut-off points, and even selective...

Metabolomics for predicting hyperglycemia in pregnancy: a protocol for a systematic review and potential meta-analysis

Bianca Nicolosi, Debora Leite, Jussara Mayrink, Renato Souza, José Cecatti & Iracema Calderon
Abstract Background Hyperglycemia in pregnancy (HIP) has been recently differentiated between diabetes in pregnancy (DIP) and gestational diabetes mellitus (GDM). The proposed protocol is relevant, and clinical concern is due to the higher risk of adverse pregnancy outcomes (APO) and long-term effects on both the mother and the fetus. Fasting plasma glucose level (FPG) and oral glucose tolerance test (OGTT) are current diagnostic tools. However, controversy persists concerning diagnostic criteria, cut-off points, and even selective...

Additional file 1: of Metabolomics for predicting hyperglycemia in pregnancy: a protocol for a systematic review and potential meta-analysis

Bianca Nicolosi, Debora Leite, Jussara Mayrink, Renato Souza, JosĂŠ Cecatti & Iracema Calderon
Metabolomics for predicting gestational diabetes mellitus: protocol for a systematic review and meta-analysis. (DOC 60 kb)

Additional file 1: of Metabolomics for predicting hyperglycemia in pregnancy: a protocol for a systematic review and potential meta-analysis

Bianca Nicolosi, Debora Leite, Jussara Mayrink, Renato Souza, JosĂŠ Cecatti & Iracema Calderon
Metabolomics for predicting gestational diabetes mellitus: protocol for a systematic review and meta-analysis. (DOC 60 kb)

Noninvasive determination of toxic stress biomarkers by high-throughput screening of photoautotrophic cell suspension cultures with multicolor fluorescence imaging

Anna Segečová, María Pérez-Bueno, Matilde Barón, Jan Červený & Thomas Roitsch
Abstract Background With increasing pollution, herbicide application and interest in plant phenotyping, sensors capturing early responses to toxic stress are demanded for screening susceptible or resistant plant varieties. Standard toxicity tests on plants are laborious, demanding in terms of space and material, and the measurement of growth-inhibition based endpoints takes relatively long time. The aim of this work was to explore the potential of photoautotrophic cell suspension cultures for high-throughput early toxicity screening based on...

MOESM1 of Noninvasive determination of toxic stress biomarkers by high-throughput screening of photoautotrophic cell suspension cultures with multicolor fluorescence imaging

Anna Segečová, María Pérez-Bueno, Matilde Barón, Jan Červený & Thomas Roitsch
Additional file 1. Additional line graphs, spider graphs, PCA biplots and table of PCA loadings for all tested concentrations and time points, visual effects on representative samples, flowchart of experimental design and scheme of data organization and inputs used for analyses.

MOESM1 of Noninvasive determination of toxic stress biomarkers by high-throughput screening of photoautotrophic cell suspension cultures with multicolor fluorescence imaging

Anna Segečová, María Pérez-Bueno, Matilde Barón, Jan Červený & Thomas Roitsch
Additional file 1. Additional line graphs, spider graphs, PCA biplots and table of PCA loadings for all tested concentrations and time points, visual effects on representative samples, flowchart of experimental design and scheme of data organization and inputs used for analyses.

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