260,427 Works

Malaria tropica infection circumvents immunity by hybrid ABO blood group phenotype formation

Peter Arend
Although the process of pathogen transmission across species barriers has not yet been completely elucidated, species barriers are most likely overcome in malaria tropica infection. The abundant serine syntheses of the protozoan parasite Plasmodium falciparum, which lacks the ability to perform O-GalNAc glycosylation and does not survive outside its metazoan host, hypothetically obtain access to the metazoan host’s evolutionarily first protein glycosylation (or serologically A-like, O-GalNAcα1-Ser/Thr-R, “T nouvelle” (Tn) antigen formation). In humans, this genetically...

Two new 12-membered macrolides from the endophytic fungal strain Cladosprium colocasiae A801 of Callistemon viminalis

Hong-Xin Liu, Hai-Bo Tan, Sai-Ni Li, Yu-Chan Chen, Hao-Hua Li, Sheng-Xiang Qiu & Wei-Min Zhang
Two new polyketide metabolites, the 12-membered macrolides 4-hydroxy-12-methyloxacyclododecane-2,5,6-trione (1) and 12-methyloxacyclododecane-2,5,6-trione (2), were isolated from the endophytic fungal strain Cladosprium colocasiae A801 of the plant Callistemon viminalis, together with five known derivatives. Their structures were fully characterized by means of detailed spectroscopic analysis for new structures, and in comparison with published data for known compounds. The antibacterial, cytotoxic, and α-glucosidase inhibitory activities of the new compounds 1 and 2 were evaluated.

Potent leishmanicidal and antibacterial metabolites from Olea ferruginea

Salman Zafar, Faiz -Ur-Rehman, Zafar Ali Shah, Abdur Rauf, Aziz Khan, Muhammad Humayun Khan, Khaliq Ur Rahman, Serab Khan, Abid Ullah & Farzana Shaheen
Inspired from the leishmanicidal and antibacterial potential of the fractions obtained from the crude extract of Olea ferruginea stem, the anti-leishmanial ethyl acetate fraction was subjected to chromatographic separation, leading to the isolation of a new compound ferruginan (1) and a known compound (+)- cycloolivil (2). The structures of 1 and 2 were determined by various spectroscopic techniques and were assayed for their in vitro antibacterial and leishmanicidal potential. Compound 1 showed 75% inhibition after...

Sesquiterpenoids from the roots of Croton crassifolius

Jin-Long Tian, Chen-Xi Li, Xin-Yue Shang, Xue-Wen Hou, Yan Zhang, Ling-Zhi Li, Xiao-Xiao Huang & Shao-Jiang Song
Phytochemical investigation of Croton crassifolius roots afforded five sesquiterpenes (1–5), including two new sesquiterpenes 6S-hydroxy-cyperenoic acid (1) and crassifterpenoid A (5), together with three known compounds (2–4). The structures of the new compounds were determined by comprehensive spectroscopic methods, and their absolute configurations were determined by quantum chemical ECD calculation. Crassifterpenoid A (5) is the first germacrane-type sesquiterpene isolated from C. crassifolius, which enriched the diversity of chemical constituents in Croton crassifolius. In addition, the...

Potent leishmanicidal and antibacterial metabolites from Olea ferruginea

Salman Zafar, Faiz -Ur-Rehman, Zafar Ali Shah, Abdur Rauf, Aziz Khan, Muhammad Humayun Khan, Khaliq Ur Rahman, Serab Khan, Abid Ullah & Farzana Shaheen
Inspired from the leishmanicidal and antibacterial potential of the fractions obtained from the crude extract of Olea ferruginea stem, the anti-leishmanial ethyl acetate fraction was subjected to chromatographic separation, leading to the isolation of a new compound ferruginan (1) and a known compound (+)- cycloolivil (2). The structures of 1 and 2 were determined by various spectroscopic techniques and were assayed for their in vitro antibacterial and leishmanicidal potential. Compound 1 showed 75% inhibition after...

Two new 12-membered macrolides from the endophytic fungal strain Cladosprium colocasiae A801 of Callistemon viminalis

Hong-Xin Liu, Hai-Bo Tan, Sai-Ni Li, Yu-Chan Chen, Hao-Hua Li, Sheng-Xiang Qiu & Wei-Min Zhang
Two new polyketide metabolites, the 12-membered macrolides 4-hydroxy-12-methyloxacyclododecane-2,5,6-trione (1) and 12-methyloxacyclododecane-2,5,6-trione (2), were isolated from the endophytic fungal strain Cladosprium colocasiae A801 of the plant Callistemon viminalis, together with five known derivatives. Their structures were fully characterized by means of detailed spectroscopic analysis for new structures, and in comparison with published data for known compounds. The antibacterial, cytotoxic, and α-glucosidase inhibitory activities of the new compounds 1 and 2 were evaluated.

Four new C20-diterpenoid alkaloids from Aconitum rotundifolium

Ji-Fa Zhang, Yu Li, Feng Gao, Lian-Hai Shan & Xian-Li Zhou
Four new C20-diterpenoid alkaloids, rotundifosines D-G (1–4), along with eight known ones (5–12) were isolated from the whole plant of Aconitum rotundifolium Kar. & Kir. The structures of the compounds were elucidated on the basis of spectroscopic analyses, including HR-ESI-MS and 1D, 2D NMR. Rotundifosine F (3) is a rare C20-diterpenoid alkaloid with quaternary ammonium salt. Alkaloids 1–4, 5, 6, 9, and 12 were evaluated for cytotoxicity against MCF-7, HCT-116 and HepG2 human cancer cell...

A new ionone derivative from the leaves of Picrasma quassioides

Chang Liu, Rong-Rong Cheng, Zhu-Zhen Han, Ying-Bo Yang, Yue Zhou, Li Yang & Zheng-Tao Wang
Nigakialcohol A (1), as unusual cyclization ionone derivative, together with eight known ones (2–9), were isolated from the leaves of Picrasma quassioides (D. Don) Benn (Simaroubaceae). Their structures were elucidated by extensive spectroscopic analyses and comparison with literature data. Compound 2 showed a weak inhibitory effect on NO production at non-cytotoxic concentration (100 μM) with inhibitory rate of 59%, and thus it should be regarded as potential anti-inflammatory agents.

A new ionone derivative from the leaves of Picrasma quassioides

Chang Liu, Rong-Rong Cheng, Zhu-Zhen Han, Ying-Bo Yang, Yue Zhou, Li Yang & Zheng-Tao Wang
Nigakialcohol A (1), as unusual cyclization ionone derivative, together with eight known ones (2–9), were isolated from the leaves of Picrasma quassioides (D. Don) Benn (Simaroubaceae). Their structures were elucidated by extensive spectroscopic analyses and comparison with literature data. Compound 2 showed a weak inhibitory effect on NO production at non-cytotoxic concentration (100 μM) with inhibitory rate of 59%, and thus it should be regarded as potential anti-inflammatory agents.

Four new C20-diterpenoid alkaloids from Aconitum rotundifolium

Ji-Fa Zhang, Yu Li, Feng Gao, Lian-Hai Shan & Xian-Li Zhou
Four new C20-diterpenoid alkaloids, rotundifosines D-G (1–4), along with eight known ones (5–12) were isolated from the whole plant of Aconitum rotundifolium Kar. & Kir. The structures of the compounds were elucidated on the basis of spectroscopic analyses, including HR-ESI-MS and 1D, 2D NMR. Rotundifosine F (3) is a rare C20-diterpenoid alkaloid with quaternary ammonium salt. Alkaloids 1–4, 5, 6, 9, and 12 were evaluated for cytotoxicity against MCF-7, HCT-116 and HepG2 human cancer cell...

A new menthane-type monoterpenoid from fermented Illigera aromatica with Clonostachys rogersoniana 828H2

Jian-Wei Dong, Le Cai, Xue-Jiao Li, Ya-Xian Shi, Jia-Peng Wang, Rui-Feng Mei & Zhong-Tao Ding
A new menthane-type monoterpenoid, illigerate E (1), as well as two known ones, (1R*,3R*,4S*,6R*)-6,8-dihydroxymenthol (2) and cis-4-hydroxy-5-(1-hydroxy-1-methylethyl)-2-methyl-2-cyclohexene-1-one (3), were isolated from fermented Illigera aromatica with Clonostachys rogersoniana 828H2. Their structures were identified by HRESIMS and 1D/2D NMR spectra. Their inhibitory effects of NO production in RAW 264.7 macrophages were estimated.

A new menthane-type monoterpenoid from fermented Illigera aromatica with Clonostachys rogersoniana 828H2

Jian-Wei Dong, Le Cai, Xue-Jiao Li, Ya-Xian Shi, Jia-Peng Wang, Rui-Feng Mei & Zhong-Tao Ding
A new menthane-type monoterpenoid, illigerate E (1), as well as two known ones, (1R*,3R*,4S*,6R*)-6,8-dihydroxymenthol (2) and cis-4-hydroxy-5-(1-hydroxy-1-methylethyl)-2-methyl-2-cyclohexene-1-one (3), were isolated from fermented Illigera aromatica with Clonostachys rogersoniana 828H2. Their structures were identified by HRESIMS and 1D/2D NMR spectra. Their inhibitory effects of NO production in RAW 264.7 macrophages were estimated.

Sesquiterpenoids from the roots of Croton crassifolius

Jin-Long Tian, Chen-Xi Li, Xin-Yue Shang, Xue-Wen Hou, Yan Zhang, Ling-Zhi Li, Xiao-Xiao Huang & Shao-Jiang Song
Phytochemical investigation of Croton crassifolius roots afforded five sesquiterpenes (1–5), including two new sesquiterpenes 6S-hydroxy-cyperenoic acid (1) and crassifterpenoid A (5), together with three known compounds (2–4). The structures of the new compounds were determined by comprehensive spectroscopic methods, and their absolute configurations were determined by quantum chemical ECD calculation. Crassifterpenoid A (5) is the first germacrane-type sesquiterpene isolated from C. crassifolius, which enriched the diversity of chemical constituents in Croton crassifolius. In addition, the...

A new labdane-type diterpenoid from Leonurus japonicus

Min-Hong Jiang, Yue Hu, Lin Jiao, Pan Dong, Sheng Yin, Zhi-Min Zhao, De-Po Yang & Dong-Mei Wang
Five fractions prepared from the crude extract of Leonurus japonicus were examined in order to determine their cytotoxic potential. Under the bioassay guidance, a new labdane-type diterpenoid (1), and nine known ones (2–10) along with a seco-labdane (11) were isolated from the aerial parts of Leonurus japonicus. The structure elucidation was primarily based on comprehensive spectroscopic analyses, including HRESIMS, IR, and 1D and 2D NMR methods. Compound 4 (6β-hydroxy-15,16-epoxylabda-8,13(16),14-trien-7-one) exhibited potential cytotoxicity against HeLa cell...

Three new polyketides from Ascotricha sp. ZJ-M-5 by the OSMAC strategy

Ya-Jing Dong, Guo-Mei Hou, Bin Lin, Dan-Yi Li & Zhan-Lin Li
Three new compounds, ascotrichols A-B (1–2) and ascotrichrone A (3), along with two known isocoumarin derivatives (4–5), were isolated from the solid culture of a sea mud-derived fungus, Ascotricha sp. ZJ-M-5 on rice media. The structures were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR data, and the absolute configurations were determined by electronic circular dichroism and biogenetic correlation.

A new labdane-type diterpenoid from Leonurus japonicus

Min-Hong Jiang, Yue Hu, Lin Jiao, Pan Dong, Sheng Yin, Zhi-Min Zhao, De-Po Yang & Dong-Mei Wang
Five fractions prepared from the crude extract of Leonurus japonicus were examined in order to determine their cytotoxic potential. Under the bioassay guidance, a new labdane-type diterpenoid (1), and nine known ones (2–10) along with a seco-labdane (11) were isolated from the aerial parts of Leonurus japonicus. The structure elucidation was primarily based on comprehensive spectroscopic analyses, including HRESIMS, IR, and 1D and 2D NMR methods. Compound 4 (6β-hydroxy-15,16-epoxylabda-8,13(16),14-trien-7-one) exhibited potential cytotoxicity against HeLa cell...

Three new polyketides from Ascotricha sp. ZJ-M-5 by the OSMAC strategy

Ya-Jing Dong, Guo-Mei Hou, Bin Lin, Dan-Yi Li & Zhan-Lin Li
Three new compounds, ascotrichols A-B (1–2) and ascotrichrone A (3), along with two known isocoumarin derivatives (4–5), were isolated from the solid culture of a sea mud-derived fungus, Ascotricha sp. ZJ-M-5 on rice media. The structures were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR data, and the absolute configurations were determined by electronic circular dichroism and biogenetic correlation.

Acylphloroglucinols as kinase inhibitors from Sargassum nigrifoloides

Cheng-Shi Jiang, Yin-Yin Wang, Jin-Tong Song & Jin-Hai Yu
Three new acylphloroglucinols (1–3) and four known biosynthetically related analogs (4–7) were isolated from the ethanol extract of a brown alga Sargassum nigrifoloides. Structures for 1–7 were characterized via detailed spectroscopic analyses especially 2D NMR data. Screening of these compounds in Alzheimer’s diseases-related bioassays revealed moderate inhibitory activities against two therapeutically important kinases, CDK5 and GSK3β. A preliminary structure–activity relationship was also discussed.

Acylphloroglucinols as kinase inhibitors from Sargassum nigrifoloides

Cheng-Shi Jiang, Yin-Yin Wang, Jin-Tong Song & Jin-Hai Yu
Three new acylphloroglucinols (1–3) and four known biosynthetically related analogs (4–7) were isolated from the ethanol extract of a brown alga Sargassum nigrifoloides. Structures for 1–7 were characterized via detailed spectroscopic analyses especially 2D NMR data. Screening of these compounds in Alzheimer’s diseases-related bioassays revealed moderate inhibitory activities against two therapeutically important kinases, CDK5 and GSK3β. A preliminary structure–activity relationship was also discussed.

Merged fungal ITS reads

Max Emil Schön, Kay Nieselt & Sigisfredo Garnica
Reads clipped and paired-end reads merged with Clip&Merge

HKU Repeatability in HK Research 2017

Scott Edmunds, Chui Ling Kee, LI MAN KI, Dongwei Wang, Jiaye Ni, Tianhui Li, Kwan Hung Pong, Yuan Ning, Hao Luo, ZIHUI LI, Yiyun Pan, Ho Wai Lam, Jiwen Fang, Ho Hin Lung, Mei Wa Chan, Yiqin Yu, Yue Lyu, Shiyuan Xu, Hoi Ling Ng, Wentao Gao, Mengyu Fan, Kwok Yung Lam, Zheyu Chen & Jiemin Zhong
Quantifying reproducibility and compliance of HKU and PLOS's open data policies, and also exploring the data availability of Scientific Reports paper, in published papers from HKU. All 2017 papers in HKU scholarhub were assessed by students in the HKU data curation class (MLIM7350).

HKU Repeatability in HK Research 2017

Scott Edmunds, Chui Ling Kee, LI MAN KI, Dongwei Wang, Jiaye Ni, Tianhui Li, Kwan Hung Pong, Yuan Ning, Hao Luo, ZIHUI LI, Yiyun Pan, Ho Wai Lam, Jiwen Fang, Ho Hin Lung, Mei Wa Chan, Yiqin Yu, Yue Lyu, Shiyuan Xu, Hoi Ling Ng, Wentao Gao, Mengyu Fan, Kwok Yung Lam, Zheyu Chen & Jiemin Zhong
Quantifying reproducibility and compliance of HKU and PLOS's open data policies, and also exploring the data availability of Scientific Reports paper, in published papers from HKU. All 2017 papers in HKU scholarhub were assessed by students in the HKU data curation class (MLIM7350).

CHIPSEQ QC

Beisi Xu
Bash script to quality control CHIPSEQ from bam file.Output bed file and cross-correlation plot for review to estimate the fragment size.
A second script will generate bigwig tracks based on estimate fragment size normalized to 15M reads.

CHIPSEQ QC

Beisi Xu
Bash script to quality control CHIPSEQ from bam file.Output bed file and cross-correlation plot for review to estimate the fragment size.
A second script will generate bigwig tracks based on estimate fragment size normalized to 15M reads.

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