Additional file 1: Supplementary Fig. 1. A circular genomic map displays the distribution of all KRAB-ZFP genes across the human genome. The width of each pink bar positively correlates to the number of KRAB-ZFP genes in a cluster.

Additional file 5 Supplementary Fig. 5. Screening efficient sgRNAs for knocking out KRAB-Zfp cluster(s) by CRISPR-Cas9. (A) The sequences and detailed information of the 11 linker-targeting sgRNAs constructed in the reporter plasmid in Fig. 4A. There are two “NGG” sites within the linker regions and they were used as PAM sites for the two sets of sgRNAs. The nucleotides identical across the whole set of sgRNA targets are labeled in black and the variable nucleotides...

Supplementary Media for Self-Supervised Deep Learning Two-Photon Microscopy

Supplementary Media for Self-Supervised Deep Learning Two-Photon Microscopy

Supplementary Media for Self-Supervised Deep Learning Two-Photon Microscopy

Additional file 4 Supplementary Fig. 4. Sequence conservation analyses of various KRAB-Zfp clusters. The amino acid sequences (top) and the corresponding nucleic acid sequences (bottom) according to the linker and nearby regions are shown in the pale purple box. The related sequences are extracted from all the KRAB-Zfps arranged in the 50 clusters across the mouse genome (a), or the indicated clusters on chromosome 2 (Clusters 1, 2, 3) (b), and on chromosome 4 (Clusters...

fabrication process of the paper based metamaterials

Animation of FIg. 3

Additional file 1: Supplementary Fig. 1. A circular genomic map displays the distribution of all KRAB-ZFP genes across the human genome. The width of each pink bar positively correlates to the number of KRAB-ZFP genes in a cluster.

Additional file 5 Supplementary Fig. 5. Screening efficient sgRNAs for knocking out KRAB-Zfp cluster(s) by CRISPR-Cas9. (A) The sequences and detailed information of the 11 linker-targeting sgRNAs constructed in the reporter plasmid in Fig. 4A. There are two “NGG” sites within the linker regions and they were used as PAM sites for the two sets of sgRNAs. The nucleotides identical across the whole set of sgRNA targets are labeled in black and the variable nucleotides...

Additional file 8 Supplementary Fig. 8. Analyses of KRAB-Zfp related Satellite DNA cluster identified in the Kauzlaric’s paper [32]. (A) Comparison of the cluster distribution of our work and the Kauzlaric’s paper. The clusters defined by both studies are pointed with red arrows; the clusters only defined by our work are pointed with blue arrows; and the clusters only defined by the Kauzlaric’s paper are pointed with green arrows, mainly including the KRAB-Zfp related Satellite...

Supplementary Media for Self-Supervised Deep Learning Two-Photon Microscopy

Additional file 6. The original blots.

Objectives: To determine the frequency and correlates of apathy in amyotrophic lateral sclerosis (ALS) and its influence on the prognosis of the disease. Methods: Three databases were searched: MEDLINE, PubMed, and Google Scholar. Quantitative synthesis of the frequency of apathy in ALS was conducted using random effects in Stata (College Station, TX). Meta-regression and subgroup analyses were conducted to investigate the association between frequency of apathy in ALS and different covariates. Results: Fifty-two studies (51...

Additional file 8 Supplementary Fig. 8. Analyses of KRAB-Zfp related Satellite DNA cluster identified in the Kauzlaric’s paper [32]. (A) Comparison of the cluster distribution of our work and the Kauzlaric’s paper. The clusters defined by both studies are pointed with red arrows; the clusters only defined by our work are pointed with blue arrows; and the clusters only defined by the Kauzlaric’s paper are pointed with green arrows, mainly including the KRAB-Zfp related Satellite...

Supplementary Media for Self-Supervised Deep Learning Two-Photon Microscopy

Supplementary Media for Self-Supervised Deep Learning Two-Photon Microscopy

Animation of FIg. 3

Additional file 4 Supplementary Fig. 4. Sequence conservation analyses of various KRAB-Zfp clusters. The amino acid sequences (top) and the corresponding nucleic acid sequences (bottom) according to the linker and nearby regions are shown in the pale purple box. The related sequences are extracted from all the KRAB-Zfps arranged in the 50 clusters across the mouse genome (a), or the indicated clusters on chromosome 2 (Clusters 1, 2, 3) (b), and on chromosome 4 (Clusters...

Additional file 6 Supplementary Fig. 6. Specificity tests of sgRNAs in qPCR assays. qPCR results for detecting the intergenetic regions between non-target KRAB-Zfps using the genomic DNA templates extracted from the cells expressing the indicated sgRNA before and after gene editing (−Dox and + Dox). The set of data are correlated to Fig. 5B.

Supplementary Media for Self-Supervised Deep Learning Two-Photon Microscopy

fabrication process of the paper based metamaterials

Additional file 1: Supp Fig. 1. A clinico-diagnositicflowchart of the antenatal management in different cases. SuppFig. 2. The differential expression mRNAswere verified by RT-PCR in TOF patients and Controls.

Supplementary Media for Self-Supervised Deep Learning Two-Photon Microscopy

Additional file 6. The original blots.