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Additional file 2: of Differential effects of specific cathepsin S inhibition in biocompartments from patients with primary Sjögren syndrome

Patrick Hargreaves, Douglas Daoudlarian, Michel Theron, Fabrice Kolb, Marianne Manchester Young, Bernhard Reis, Andre Tiaden, Bettina Bannert, Diego Kyburz & Tobias Manigold
Figure S2. Antigen-specific T cell responses in healthy controls (HC), assessed by IFN-γ/IL-17 Dual ELISPOT assays for 48 h (A) and BrdU proliferation assays for 72 h (B) following stimulation with 5 μg/ml recombinant SS-A (60kD), SS-B, influenza H3N2 or 2 μg/ml tetanus toxoid or 0.1 mg/ml SEB in the absence or presence of RO5459072 (1–100 μM). For details, see material and methods or legend of Fig. 3. (one tailed t test *, §, π,...

Additional file 3: of Differential effects of specific cathepsin S inhibition in biocompartments from patients with primary Sjögren syndrome

Patrick Hargreaves, Douglas Daoudlarian, Michel Theron, Fabrice Kolb, Marianne Manchester Young, Bernhard Reis, Andre Tiaden, Bettina Bannert, Diego Kyburz & Tobias Manigold
Figure S3. Stability of CatS activity in tear fluid upon storage at − 80 °C. Specifically, tear fluid was stored at − 80 °C on the day of sample acquisition for 7–8 days until the first measurement. After testing, they were re-stored at − 80 °C over a period of 6 (HC1 and pSS2) and 8 (pSS1) weeks, respectively, until the second measurement. As indicated, CatS activity was stable, with a trend towards higher levels...

Additional file 1: of Differential effects of specific cathepsin S inhibition in biocompartments from patients with primary Sjögren syndrome

Patrick Hargreaves, Douglas Daoudlarian, Michel Theron, Fabrice Kolb, Marianne Manchester Young, Bernhard Reis, Andre Tiaden, Bettina Bannert, Diego Kyburz & Tobias Manigold
Figure S1. Ex vivo effects of RO5459072. RO5459072 led to a dose-dependent p10 accumulation in B cells when compared to monocytes (A). In addition, 80 mM of RO5459072 led to significantly decreased CatS ratios in tear fluid, but not other biocompartments, of pSS after an incubation of 180 min (B, two-tailed t test, ** p

Additional file 1: of Differential effects of specific cathepsin S inhibition in biocompartments from patients with primary Sjögren syndrome

Patrick Hargreaves, Douglas Daoudlarian, Michel Theron, Fabrice Kolb, Marianne Manchester Young, Bernhard Reis, Andre Tiaden, Bettina Bannert, Diego Kyburz & Tobias Manigold
Figure S1. Ex vivo effects of RO5459072. RO5459072 led to a dose-dependent p10 accumulation in B cells when compared to monocytes (A). In addition, 80 mM of RO5459072 led to significantly decreased CatS ratios in tear fluid, but not other biocompartments, of pSS after an incubation of 180 min (B, two-tailed t test, ** p

Additional file 2: of Differential effects of specific cathepsin S inhibition in biocompartments from patients with primary Sjögren syndrome

Patrick Hargreaves, Douglas Daoudlarian, Michel Theron, Fabrice Kolb, Marianne Manchester Young, Bernhard Reis, Andre Tiaden, Bettina Bannert, Diego Kyburz & Tobias Manigold
Figure S2. Antigen-specific T cell responses in healthy controls (HC), assessed by IFN-γ/IL-17 Dual ELISPOT assays for 48 h (A) and BrdU proliferation assays for 72 h (B) following stimulation with 5 μg/ml recombinant SS-A (60kD), SS-B, influenza H3N2 or 2 μg/ml tetanus toxoid or 0.1 mg/ml SEB in the absence or presence of RO5459072 (1–100 μM). For details, see material and methods or legend of Fig. 3. (one tailed t test *, §, π,...

Additional file 3: of Differential effects of specific cathepsin S inhibition in biocompartments from patients with primary Sjögren syndrome

Patrick Hargreaves, Douglas Daoudlarian, Michel Theron, Fabrice Kolb, Marianne Manchester Young, Bernhard Reis, Andre Tiaden, Bettina Bannert, Diego Kyburz & Tobias Manigold
Figure S3. Stability of CatS activity in tear fluid upon storage at − 80 °C. Specifically, tear fluid was stored at − 80 °C on the day of sample acquisition for 7–8 days until the first measurement. After testing, they were re-stored at − 80 °C over a period of 6 (HC1 and pSS2) and 8 (pSS1) weeks, respectively, until the second measurement. As indicated, CatS activity was stable, with a trend towards higher levels...

Additional file 2: of Hemodynamically balanced congenitally corrected transposition of the great arteries with a large ventricular septal defect, and subvalvular pulmonic stenosis: a case report

Sang-Yeong Cho, Yeonyee Yoon, Wonjae Lee, Si-Hyuck Kang, Young Song, Cheong Lim, Goo-Yeong Cho & Jeong-Wook Seo
A three-dimensional model of the vertical view of the congenitally corrected transposition of the great arteries is shown. (MP4 11924 kb)

Additional file 2: of Hemodynamically balanced congenitally corrected transposition of the great arteries with a large ventricular septal defect, and subvalvular pulmonic stenosis: a case report

Sang-Yeong Cho, Yeonyee Yoon, Wonjae Lee, Si-Hyuck Kang, Young Song, Cheong Lim, Goo-Yeong Cho & Jeong-Wook Seo
A three-dimensional model of the vertical view of the congenitally corrected transposition of the great arteries is shown. (MP4 11924 kb)

Additional file 3: of Hemodynamically balanced congenitally corrected transposition of the great arteries with a large ventricular septal defect, and subvalvular pulmonic stenosis: a case report

Sang-Yeong Cho, Yeonyee Yoon, Wonjae Lee, Si-Hyuck Kang, Young Song, Cheong Lim, Goo-Yeong Cho & Jeong-Wook Seo
A three-dimensional model of the detailed anatomy of the sub-pulmonary structure and its relation to the ventricular septum is shown. (MP4 11268 kb)

Additional file 3: of Hemodynamically balanced congenitally corrected transposition of the great arteries with a large ventricular septal defect, and subvalvular pulmonic stenosis: a case report

Sang-Yeong Cho, Yeonyee Yoon, Wonjae Lee, Si-Hyuck Kang, Young Song, Cheong Lim, Goo-Yeong Cho & Jeong-Wook Seo
A three-dimensional model of the detailed anatomy of the sub-pulmonary structure and its relation to the ventricular septum is shown. (MP4 11268 kb)

Additional file 4: of Hemodynamically balanced congenitally corrected transposition of the great arteries with a large ventricular septal defect, and subvalvular pulmonic stenosis: a case report

Sang-Yeong Cho, Yeonyee Yoon, Wonjae Lee, Si-Hyuck Kang, Young Song, Cheong Lim, Goo-Yeong Cho & Jeong-Wook Seo
A three-dimensional model of the widely open aortic outflow tract is shown. The aortic outflow is separated from the stenotic pulmonary outflow tract. (MP4 5819 kb)

Additional file 1: of Hemodynamically balanced congenitally corrected transposition of the great arteries with a large ventricular septal defect, and subvalvular pulmonic stenosis: a case report

Sang-Yeong Cho, Yeonyee Yoon, Wonjae Lee, Si-Hyuck Kang, Young Song, Cheong Lim, Goo-Yeong Cho & Jeong-Wook Seo
A three-dimensional model for the overview of the congenitally corrected transposition of the great arteries is shown. (MP4 13393 kb)

Additional file 4: of Hemodynamically balanced congenitally corrected transposition of the great arteries with a large ventricular septal defect, and subvalvular pulmonic stenosis: a case report

Sang-Yeong Cho, Yeonyee Yoon, Wonjae Lee, Si-Hyuck Kang, Young Song, Cheong Lim, Goo-Yeong Cho & Jeong-Wook Seo
A three-dimensional model of the widely open aortic outflow tract is shown. The aortic outflow is separated from the stenotic pulmonary outflow tract. (MP4 5819 kb)

Additional file 1: of Hemodynamically balanced congenitally corrected transposition of the great arteries with a large ventricular septal defect, and subvalvular pulmonic stenosis: a case report

Sang-Yeong Cho, Yeonyee Yoon, Wonjae Lee, Si-Hyuck Kang, Young Song, Cheong Lim, Goo-Yeong Cho & Jeong-Wook Seo
A three-dimensional model for the overview of the congenitally corrected transposition of the great arteries is shown. (MP4 13393 kb)

MOESM4 of Automated analysis of evolving interfaces during in situ electron microscopy

Nicholas Schneider, Jeung Park, Michael Norton, Frances Ross & Haim Bau
Additional file 4: Video S4. This Video shows examples of beam-mediated etching of gold nanorods (nominally 50x80 nm). The nanorods are suspended in water (pH ~7) with trace amounts of the surfactant cetrimonium bromide (CTAB) and chloroauric acid. Imaging was carried with a FEI Quanta 600 in STEM mode (30 keV) beam currents of 0.07 nA, beam radius of ~ 1 nm resulting in a time average dose rate of 7.4 x 106 (Gy/s) or...

MOESM5 of Automated analysis of evolving interfaces during in situ electron microscopy

Nicholas Schneider, Jeung Park, Michael Norton, Frances Ross & Haim Bau
Additional file 5: Video S5. Nanobubble formation experiments in an aqueous solution of gold nanorods with a measured pH ~ 7 and trace amounts of the surfactant cetrimonium bromide (CTAB). Imaging was performed with a Hitachi 9000 TEM (300 keV) at beam current ~ 1 nA and beam radius ~ 2 μm resulting in a dose rate of 7.5 x 107 (Gy/s) or 20 (e−/Å2-s). The bubbles are discussed in Grogan et al. [26] and...

MOESM5 of Automated analysis of evolving interfaces during in situ electron microscopy

Nicholas Schneider, Jeung Park, Michael Norton, Frances Ross & Haim Bau
Additional file 5: Video S5. Nanobubble formation experiments in an aqueous solution of gold nanorods with a measured pH ~ 7 and trace amounts of the surfactant cetrimonium bromide (CTAB). Imaging was performed with a Hitachi 9000 TEM (300 keV) at beam current ~ 1 nA and beam radius ~ 2 μm resulting in a dose rate of 7.5 x 107 (Gy/s) or 20 (e−/Å2-s). The bubbles are discussed in Grogan et al. [26] and...

MOESM1 of Automated analysis of evolving interfaces during in situ electron microscopy

Nicholas Schneider, Jeung Park, Michael Norton, Frances Ross & Haim Bau
Additional file 1: Video S1. Code Sample Walkthrough This narrated screencast is a walkthrough of Code S1, which shows the fundamentals of the image processing. Using Video S2 and images from the experiments described in the main text, we recreate the processed images shown in Figs. 1b, 2 of the main text. The video is hosted on the journal website and on YouTube under a Creative Commons CopyRight License [ http://bit.ly/1MWTkge ].

MOESM4 of Automated analysis of evolving interfaces during in situ electron microscopy

Nicholas Schneider, Jeung Park, Michael Norton, Frances Ross & Haim Bau
Additional file 4: Video S4. This Video shows examples of beam-mediated etching of gold nanorods (nominally 50x80 nm). The nanorods are suspended in water (pH ~7) with trace amounts of the surfactant cetrimonium bromide (CTAB) and chloroauric acid. Imaging was carried with a FEI Quanta 600 in STEM mode (30 keV) beam currents of 0.07 nA, beam radius of ~ 1 nm resulting in a time average dose rate of 7.4 x 106 (Gy/s) or...

MOESM2 of Automated analysis of evolving interfaces during in situ electron microscopy

Nicholas Schneider, Jeung Park, Michael Norton, Frances Ross & Haim Bau
Additional file 2: Video S2. This Video shows gold crystal growth in the nanoaquarium [41] with transmitted beam current of 7.8 nA spread over 2 μm diameter resulting in a dose rate of 5.9x108 (Gy/s) or 160 (e−/Å2-s). Stock solutions of chloroauric acid (Sigma-Aldrich) were made by dissolving the salt in deionized (DI) water at concentration of 20 mM. The liquid thickness within the imaged region is 50-100 nm. Irradiation by the electron beam creates...

MOESM2 of Automated analysis of evolving interfaces during in situ electron microscopy

Nicholas Schneider, Jeung Park, Michael Norton, Frances Ross & Haim Bau
Additional file 2: Video S2. This Video shows gold crystal growth in the nanoaquarium [41] with transmitted beam current of 7.8 nA spread over 2 μm diameter resulting in a dose rate of 5.9x108 (Gy/s) or 160 (e−/Å2-s). Stock solutions of chloroauric acid (Sigma-Aldrich) were made by dissolving the salt in deionized (DI) water at concentration of 20 mM. The liquid thickness within the imaged region is 50-100 nm. Irradiation by the electron beam creates...

MOESM1 of Automated analysis of evolving interfaces during in situ electron microscopy

Nicholas Schneider, Jeung Park, Michael Norton, Frances Ross & Haim Bau
Additional file 1: Video S1. Code Sample Walkthrough This narrated screencast is a walkthrough of Code S1, which shows the fundamentals of the image processing. Using Video S2 and images from the experiments described in the main text, we recreate the processed images shown in Figs. 1b, 2 of the main text. The video is hosted on the journal website and on YouTube under a Creative Commons CopyRight License [ http://bit.ly/1MWTkge ].

MOESM3 of Automated analysis of evolving interfaces during in situ electron microscopy

Nicholas Schneider, Jeung Park, Michael Norton, Frances Ross & Haim Bau
Additional file 3: Video S3. This Video shows copper electrodeposition in the nanoaquarium [41], operating in a three-terminal configuration with Pt electrodes controlled by a Gamry potentiostat. Aqueous solutions of 0.1 M copper sulfate with 0.18 M sulfuric acid solutions were prepared in doubly deionized water and were not deaerated before use. The interface morphology evolution was imaged at video rate (30 frames per second) during galvanostatic deposition with a total current of 400 nA...

MOESM3 of Automated analysis of evolving interfaces during in situ electron microscopy

Nicholas Schneider, Jeung Park, Michael Norton, Frances Ross & Haim Bau
Additional file 3: Video S3. This Video shows copper electrodeposition in the nanoaquarium [41], operating in a three-terminal configuration with Pt electrodes controlled by a Gamry potentiostat. Aqueous solutions of 0.1 M copper sulfate with 0.18 M sulfuric acid solutions were prepared in doubly deionized water and were not deaerated before use. The interface morphology evolution was imaged at video rate (30 frames per second) during galvanostatic deposition with a total current of 400 nA...

Additional file 1: of Co-morbidity of progressive supranuclear palsy and amyotrophic lateral sclerosis: a clinical-pathological case report

Koji Fujita, Tomoyasu Matsubara, Ryosuke Miyamoto, Hiroyuki Sumikura, Toshiaki Takeuchi, Keiko Maruyama Saladini, Toshitaka Kawarai, Hiroyuki Nodera, Fukashi Udaka, Kodai Kume, Hiroyuki Morino, Hideshi Kawakami, Masato Hasegawa, Ryuji Kaji, Shigeo Murayama & Yuishin Izumi
Brain computed tomography. (PPTX 3124 kb)

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