633,612 Works
Supplementary information for: High-throughput phenotyping of cell-to-cell interactions in gel microdroplet pico-cultures
Juliette Ohan, Benjamin Pellea, , & Shawn R. Starkenburg
Supplementary Table 1. Cell input modifications for GMD capture.
Bacterial and algal concentrations were adjusted to cell concentrations
OD600, OD750 in 1x PBS and 100 µl of the resultant cell suspension (or 50 +
50 µL for abGMDs) was used as cell input for initial capture in the CelGel
Matrix prior to sorting. This ratio of bacteria-to-matrix or algae-to-matrix
(# cells/GMD) was verified by microscopy; data not presented. “aGMD” = algae
only GMDs, “bGMD” =...
Supplementary information for: High-throughput phenotyping of cell-to-cell interactions in gel microdroplet pico-cultures
Juliette Ohan, Benjamin Pellea, , & Shawn R. Starkenburg
Supplementary Table 1. Cell input modifications for GMD capture.
Bacterial and algal concentrations were adjusted to cell concentrations
OD600, OD750 in 1x PBS and 100 µl of the resultant cell suspension (or 50 +
50 µL for abGMDs) was used as cell input for initial capture in the CelGel
Matrix prior to sorting. This ratio of bacteria-to-matrix or algae-to-matrix
(# cells/GMD) was verified by microscopy; data not presented. “aGMD” = algae
only GMDs, “bGMD” =...
Supplementary information for: A mismatch-tolerant RT-quantitative PCR: application to broad-spectrum detection of respiratory syncytial virus
, , , Yi Zhou, Qin Chen &
Supplementary figure 1. Comparison of the new mismatch-tolerant
RT-qPCR with the assays with only Taq (conventional) and high fidelity (Q5)
DNA polymerase. A. Detection of RSV mutants; B. Detection HCoV 229E mutants.
The amplified products were detected using 2.5% agarose gel electrophoresis.
The arrow highlights the specific product. For other details, please see
Figure 3.
Supplementary
figure 2. Maximum likelihood
(ML) tree of sequences from four clinical samples. ML tree of RSV-A and RSV-B
sequences...
Supplementary information for: A mismatch-tolerant RT-quantitative PCR: application to broad-spectrum detection of respiratory syncytial virus
, , , Yi Zhou, Qin Chen &
Supplementary figure 1. Comparison of the new mismatch-tolerant
RT-qPCR with the assays with only Taq (conventional) and high fidelity (Q5)
DNA polymerase. A. Detection of RSV mutants; B. Detection HCoV 229E mutants.
The amplified products were detected using 2.5% agarose gel electrophoresis.
The arrow highlights the specific product. For other details, please see
Figure 3.
Supplementary
figure 2. Maximum likelihood
(ML) tree of sequences from four clinical samples. ML tree of RSV-A and RSV-B
sequences...
Supplementary information for: Fluidic resistance control enables high throughput establishment of mixed species biofilms
Mads Frederik Hansen, Anders Meyer Torp, Jonas Stenløkke Madsen, Henriette Lyng Røder & Mette Burmølle
Supplementary Figure 1. Viability is unaffected by short time
incubation in high viscosity medium. (A) The number of counted CFU/ml of
Pseudomonas putida and Paenibacillus amylolyticus is identical after 45 min. of
incubation in PBS and high viscosity liquid (50% glycerol). (B) Similarly, the
viability of Xanthomonas retroflexus was unaffected by incubation in high
viscosity liquid and did not change over a short time period (N=3, Error bars
represent Standard Error (SE)).
Supplementary information for: Fluidic resistance control enables high throughput establishment of mixed species biofilms
Mads Frederik Hansen, Anders Meyer Torp, Jonas Stenløkke Madsen, Henriette Lyng Røder & Mette Burmølle
Supplementary Figure 1. Viability is unaffected by short time
incubation in high viscosity medium. (A) The number of counted CFU/ml of
Pseudomonas putida and Paenibacillus amylolyticus is identical after 45 min. of
incubation in PBS and high viscosity liquid (50% glycerol). (B) Similarly, the
viability of Xanthomonas retroflexus was unaffected by incubation in high
viscosity liquid and did not change over a short time period (N=3, Error bars
represent Standard Error (SE)).
Previous Archaeological Excavations along the 'Ostlänken' Railway Corridor 1965-2012: District cooling through the saltpetre factory
Archaeology Department Östergötland County Museum
Previous Archaeological Excavations along the 'Ostlänken' Railway Corridor 1965-2012: District cooling through the saltpetre factory
Previous Archaeological Excavations along the 'Ostlänken' Railway Corridor 1965-2012: Skatepark at Himmelstalund sports ground
UV Öst Swedish National Heritage Board
Skatepark at Himmelstalund sports ground
Dissolved and dissolvable iron concentrations and ligand characteristics in samples taken during POLARSTERN expedition ARK-XXII/2 to the Arctic Ocean, supplement to: Thuróczy, Charles-Edouard; Gerringa, Loes J A; Klunder, Maarten B; Laan, Patrick; Le Guitton, M; de Baar, Hein J W (2011): Distinct trends in the speciation of iron between the shallow shelf seas and the deep basins of the Arctic Ocean. Journal of Geophysical Research, 116(C10), C10009
Charles-Edouard Thuróczy, Loes J A Gerringa, Maarten B Klunder, Patrick Laan, M Le Guitton & Hein J W de Baar
The speciation of iron was investigated in three shelf seas and three deep basins of the Arctic Ocean in 2007. The dissolved fraction (<0.2 µm) and a fraction < 1000 kDa were considered here. In addition, unfiltered samples were analyzed. Between 74 and 83% of dissolved iron was present in the fraction < 1000 kDa at all stations and depth, except at the chlorophyll maximum (42-64%). Distinct trends in iron concentrations and ligand characteristics were...
Calcite accumulation in the eastern equatorial Pacific, supplement to: Loubere, Paul; Mekik, Figen; Francois, Roger; Pichat, Sylvain (2004): Export fluxes of calcite in the eastern equatorial Pacific from the Last Glacial Maximum to present. Paleoceanography, 19(2), PA2018
Paul Loubere, Figen Mekik, Roger Francois & Sylvain Pichat
The eastern equatorial Pacific (EEP) is an important center of biological productivity, generating significant organic carbon and calcite fluxes to the deep ocean. We reconstructed paleocalcite flux for the past 30,000 years in four cores collected beneath the equatorial upwelling and the South Equatorial Current (SEC) by measuring ex230Th-normalized calcite accumulation rates corrected for dissolution with a newly developed proxy for "fraction of calcite preserved". This method produced very similar results at the four sites...
Density and biomass of two copepod size fractions and various species obtained during Almirante Irizar cruises to the Drake Passage and Scotia Sea (2000-2003), supplement to: Thompson, Gustavo A; Dinofrio, Estela O; Alder, Viviana A (2011): Interannual fluctuations in copepod abundance and contribution of small forms in the Drake Passage during austral summer. Helgoland Marine Research, 66(2), 127-138
Gustavo A Thompson, Estela O Dinofrio & Viviana A Alder
The relative importance of small forms of copepods has been historically underestimated by the traditional use of 200-300-µm mesh nets. This work quantified the distribution and abundance of copepods, considering two size fractions (<300 µm and >300 µm), in superficial waters (9 m deep) of the Drake Passage and contributed to the knowledge of their interannual fluctuations among three summers. Four types of nauplii and eleven species of copepods at copepodite and adult stages were...
LBA-ECO CD-17 Secondary Forest Survey, Para and Rondonia, Brazil: 2002-2003
W.A. SALAS, D.S. ALVES, M.J. DUCEY, D.J. ZARIN & J. QI
This data set provides measurements for diameter at breast height (DBH), tree height, distance from tree stems to the furthest canopy element, and a species survey of secondary forests in Para and Rondonia, Brazil, from 2002-2003. The forest areas were defined as Type A and Type B stands. Measurements were made in the overstory, understory, and midstory of each stand.
Type A stands were sampled intensively, with the goal of providing high-fidelity spatial information about...
Registration Year
Affiliations
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Imperial College London447
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Cardiff University48
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Ca Foscari University of Venice16
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London School of Hygiene & Tropical Medicine15
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Istituto Nazionale di Geofisica e Vulcanologia (INGV)15
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Vanderbilt University13
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Delft University of Technology12
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Escuela Internacional de Negocios y Desarrollo Empresarial de Colombia10
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University of Beira Interior9
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Polytechnic Institute of Viseu8