132 Works

EVI1 expression in early-stage breast cancer patients treated with neoadjuvant chemotherapy

Jonas Leichsenring, Valentina Vladimirova, Christine Solbach, Thomas Karn, Beyhan Ataseven, Bruno Valentin Sinn, Jana Barinoff, Volkmar Müller, Jens-Uwe Blohmer, Christian Schem, Knut Engels, Frederik Marmé, Annette Fisseler-Eckhoff, Peter A. Fasching, Elmar Stickeler, Marion van Mackelenbergh, Carsten Denkert, Albrecht Stenzinger, Sibylle Loibl & Stefan Gröschel
Abstract Background Overexpression of the EVI1 (ecotropic viral integration site 1) oncogene has recently been implicated as a prognostic factor in breast cancer (BC), particularly in triple-negative BC (TNBC). In this study we aimed to investigate frequency and clinical relevance of EVI1 expression in newly diagnosed BC treated with neoadjuvant chemotherapy. Methods EVI1 expression was determined by immunohistochemistry using H-score as a cumulative measurement of protein expression in pretherapeutic biopsies of BC patients treated with...

Risk factors and outcomes of postoperative stroke in surgical treatment for giant intracranial aneurysms

Hao Wang, Junlin Lu, Xin Chen & Qiang Hao
Abstract Background Giant intracranial aneurysms (GIAs) are challenges for surgical treatment. Risk factors of postoperative stroke remain unclear. This study aims to investigate the predictors of postoperative stroke in GIAs and the impact of stroke on outcomes. Methods We performed a retrospective medical record review of patients with GIAs who received microsurgery at our institution between 2011 and 2018. Multivariate logistic regression analyses were carried out to identify risk factors for postoperative stroke. The clinical...

The dynamic changes and sex differences of 147 immune-related proteins during acute COVID-19 in 580 individuals

Guillaume Butler-Laporte, Edgar Gonzalez-Kozlova, Chen-Yang Su, Sirui Zhou, Tomoko Nakanishi, Elsa Brunet-Ratnasingham, David Morrison, Laetitia Laurent, Jonathan Afilalo, Marc Afilalo, Danielle Henry, Yiheng Chen, Julia Carrasco-Zanini, Yossi Farjoun, Maik Pietzner, Nofar Kimchi, Zaman Afrasiabi, Nardin Rezk, Meriem Bouab, Louis Petitjean, Charlotte Guzman, Xiaoqing Xue, Chris Tselios, Branka Vulesevic, Olumide Adeleye … & J Brent Richards
Abstract Introduction Severe COVID-19 leads to important changes in circulating immune-related proteins. To date it has been difficult to understand their temporal relationship and identify cytokines that are drivers of severe COVID-19 outcomes and underlie differences in outcomes between sexes. Here, we measured 147 immune-related proteins during acute COVID-19 to investigate these questions. Methods We measured circulating protein abundances using the SOMAscan nucleic acid aptamer panel in two large independent hospital-based COVID-19 cohorts in Canada...

Mid-infrared Nb₄N₃-based superconducting nanowire single photon detectors for wavelengths up to 10 μm

Yiming Pan, Hui Zhou, XINGYU ZHANG, Huiqin Yu, Lu Zhang, Mengting Si, Hao Li, Lixing YOU & Zhen Wang
Mid-infrared (MIR) single-photon detection is emerging as an important technology for various applications. Superconducting nanowire single photon detectors (SNSPDs) fabricated with superconducting films with energy gaps of a few meV are natural broadband single-photon detectors. Recently, extending SNSPDs’ operation wavelengths into the MIR region is highly attractive. γ-Nb₄N₃ has a reduced N content and lower energy gap than the commonly used δ-NbN, making SNSPDs based on γ-Nb₄N₃ film more sensitive to low energy photons. We...

Antibiotic prescribing patterns at children’s outpatient departments of primary care institutions in Southwest China

Wenju Wang, Shitao Yu, Xunrong Zhou, Lei Wang, Xun He, Hanni Zhou & Yue Chang
Abstract Background Inappropriate use of antibiotics in children is common in many countries. The purpose of the study was to explore patterns of antibiotic prescribing in children’s outpatient clinics in primary care institutions in a province of southwest China. Methods We obtained electronic prescription data from 75 primary care institutions in Guizhou province in 2020. The classification of incorrect spectrum of antibiotics, unnecessary use and combined use of antibiotics was based on the Guiding Principle...

Multiomics analyses reveals Anaplasma phagocytophilum Ats-1 induces anti-apoptosis and energy metabolism by upregulating the respiratory chain-mPTP axis in eukaryotic mitochondria

Ruirui Li, Zhongchen Ma, Wei Zheng, Zhen Wang, Jihai Yi, Yangyang Xiao, Yong Wang & Chuangfu Chen
Abstract Background Anaplasma translocated substrate 1 (Ats-1) is an effector of type 4 secretory systems (T4SS) and the main virulence factor of Anaplasma phagocytophilum. Ats-1 is involved in the regulation of host cell biological processes, but the specific molecular mechanism of its action is unclear. Results In this study, we identified Ats-1 as involved in mitochondrial respiratory regulation of HEK293T cells by multi-omics analysis. After intracellular expression of Ats-1, adenosine triphosphate levels and the proliferation...

Educating physicians on strong opioids by descriptive versus simulated-experience formats: a randomized controlled trial

Odette Wegwarth, Claudia Spies, Wolf-Dieter Ludwig, Norbert Donner-Banzhoff, Günther Jonitz & Ralph Hertwig
Abstract Background Long-term prescriptions of strong opioids for chronic noncancer pain—which are not supported by scientific evidence—suggest miscalibrated risk perceptions among those who prescribe, dispense, and take opioids. Because risk perceptions and behaviors can differ depending on whether people learn about risks through description or experience, we investigated the effects of descriptive versus simulated-experience educative formats on physicians’ risk perceptions of strong opioids and their prescription behavior for managing chronic noncancer pain. Methods Three hundred...

Digitally-supported patient-centered asynchronous outpatient follow-up in rheumatoid arthritis - an explorative qualitative study

Ramona Stenzel, Katharina Hadaschik, Susann May, Manuel Grahammer, Hannah Labinsky, Martin Welcker, Johannes Hornig, Gerlinde Bendzuck, Corinna Elling-Audersch, Ulrike Erstling, Patricia Steffens Korbanka, Nicolas Vuillerme, Martin Heinze, Gerhard Krönke, Georg Schett, Ann-Christin Pecher, Martin Krusche, Johanna Mucke, Johannes Knitza & Felix Muehlensiepen
Abstract Objective A steadily increasing demand and decreasing number of rheumatologists push current rheumatology care to its limits. Long travel times and poor accessibility of rheumatologists present particular challenges for patients. Need-adapted, digitally supported, patient-centered and flexible models of care could contribute to maintaining high-quality patient care. This qualitative study was embedded in a randomized controlled trial (TELERA) investigating a new model of care consisting of the use of a medical app for ePRO (electronic...

Unambiguous spectral characterization on triplet energy transfer from quantum dots mediated by hole transfer competing with other carrier dynamics

Lei Wang, Gaoyuan Yang, Boyu Zhang, Xin Zhang, Dezheng Liu, Ying Liang & Guijie Liang
Triplet generation by QDs-sensitized molecules emerges great potential in many applications. However, the mechanism of triplet energy transfer (TET) is still fuzzy especially due to complicated energy level alignment of QDs and molecules or trap states in QDs. Here, CdSe QDs and 5-tetracene carboxylic acid (TCA) molecules are selected as the triplet donor and acceptor, respectively, to form a TET system. By tuning the band gap of CdSe, the CdSe-TCA complex is exactly designed to...

Impaired response of blood neutrophils to cell-death stimulus differentiates AQP4-IgG-seropositive NMOSD from MOGAD

Maria Schroeder-Castagno, Alba Del Rio-Serrato, Andreas Wilhelm, Silvina Romero-Suárez, Patrick Schindler, Cesar Alvarez-González, Ankelien-Solveig Duchow, Judith Bellmann-Strobl, Klemens Ruprecht, Maria Hastermann, Gerald Grütz, Brigitte Wildemann, Sven Jarius, Tanja Schmitz-Hübsch, Friedemann Paul & Carmen Infante-Duarte
Abstract Background In neuromyelitis optica spectrum disorders (NMOSD) and myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD), neutrophils are found in CNS lesions. We previously demonstrated that NMOSD neutrophils show functional deficiencies. Thus, we hypothesized that neutrophil accumulation in the CNS may be facilitated by impairments affecting mechanisms of neutrophil death. Objective To evaluate cell death in blood neutrophils from aquaporin-4 (AQP4)-IgG-seropositive NMOSD and MOGAD patients as well as matched healthy controls (HC) using in vitro assays....

The use of video consultations to support orthopedic patients' treatment at the interface of a clinic and general practitioners

Estel K, Richter L, Weber G, Fellmer F, Märdian S, Willy C & Back DA
Abstract Introduction Video consultations have proven to be a powerful support tool for patient–doctor interactions in general, not only during the COVID-19 pandemic. This study analyzed the feasibility and usefulness of orthopedic telemedical consultations (OTCs) for orthopedic patients at the interface of a clinic and general practitioners. Methods The study was carried out at an orthopedic department of a German hospital between April 2020 and October 2020. After written informed consent was obtained, general practitioners...

The use of video consultations to support orthopedic patients' treatment at the interface of a clinic and general practitioners

Estel K, Richter L, Weber G, Fellmer F, Märdian S, Willy C & Back DA
Abstract Introduction Video consultations have proven to be a powerful support tool for patient–doctor interactions in general, not only during the COVID-19 pandemic. This study analyzed the feasibility and usefulness of orthopedic telemedical consultations (OTCs) for orthopedic patients at the interface of a clinic and general practitioners. Methods The study was carried out at an orthopedic department of a German hospital between April 2020 and October 2020. After written informed consent was obtained, general practitioners...

The neuropeptide PACAP alleviates T. gondii infection-induced neuroinflammation and neuronal impairment

Caio Andreeta Figueiredo, Henning Peter Düsedau, Johannes Steffen, Stefanie Ehrentraut, Miklos P. Dunay, Gabor Toth, Dora Reglödi, Markus M. Heimesaat & Ildiko Rita Dunay
Abstract Background Cerebral infection with the protozoan Toxoplasma gondii (T. gondii) is responsible for inflammation of the central nervous system (CNS) contributing to subtle neuronal alterations. Albeit essential for brain parasite control, continuous microglia activation and recruitment of peripheral immune cells entail distinct neuronal impairment upon infection-induced neuroinflammation. PACAP is an endogenous neuropeptide known to inhibit inflammation and promote neuronal survival. Since PACAP is actively transported into the CNS, we aimed to assess the impact...

MiRNA/mRNA network topology in hepatitis virus B-related liver cirrhosis reveals miR-20a-5p/340-5p as hubs initiating fibrosis

Heng Yao, Peng Li, Jiaojiao Xin, Xi Liang, Jing Jiang, Dongyan Shi, Jiang Li, Hozeifa Mohamed Hassan, Xin Chen & Jun Li
Abstract Background The pathophysiology of hepatitis B-related liver cirrhosis (HBV-LC) remains unclear. This study aimed to explore the disease mechanisms using topological analysis of the miRNA/mRNA network. Methods Paired miRNA/mRNA sequencing was performed with thirty-three peripheral blood mononuclear cell samples (LC, n = 9; chronic hepatitis B, n = 12; normal controls, n = 12) collected from a prospective cohort to identify the miRNA/mRNA network. Topological features and functional implications of the network were analyzed...

Effect of Liuzijue on pulmonary rehabilitation in patients with chronic obstructive pulmonary disease: study protocol for a multicenter, non-randomized, prospective study

Jiaming Hu, Rundi Gao, Yiting Wang, Yan Li, Yaqin Wang, Zhen Wang & Junchao Yang
Abstract Background Traditional Chinese exercise as a new pulmonary rehabilitation technique has been increasingly used and achieved good results in pulmonary rehabilitation of chronic obstructive pulmonary disease (COPD). The aim of this study is to investigate the protective effects of Liuzijue on exercise tolerance, lung function, and quality of life in patients with COPD. Methods This study is a multicenter, non-randomized, prospective study. Patients will be divided into a control group (CG) and a Liuzijue...

Integrated analysis to identify the AC005154.6/hsa-miR-29c-3p/CCNL2 axis as a novel prognostic biomarker associated with immune infiltration in prostate cancer

Qinyu Li, Bingliang Chen, Guoda Song, Kai Zeng, Xin Chen, Jianping Miao, Xianglin Yuan, Jihong Liu, Zhihua Wang & Bo Liu
Abstract Background Prostate cancer (PCa) is currently the major malignancy in men. It is becoming increasingly clear that competitive endogenous RNA (ceRNA) regulation networks are important in a wide variety of cancers. Nevertheless, there is still much to learn about the biological functions of the ceRNA network in prostate cancer. Methods The ceRNA network was constructed using the "GDCRNATools" package. Based on survival analysis, we obtained AC005154.6/hsa-miR-29c-3p/CCNL2 for further analysis. The prognostic model based on...

Feasibility and outcome of reproducible clinical interpretation of high-dimensional molecular data: a comparison of two molecular tumor boards

Damian T. Rieke, Till de Bortoli, Peter Horak, Mario Lamping, Manuela Benary, Ivan Jelas, Gina Rüter, Johannes Berger, Marit Zettwitz, Niklas Kagelmann, Andreas Kind, Falk Fabian, Dieter Beule, Hanno Glimm, Benedikt Brors, Albrecht Stenzinger, Stefan Fröhling & Ulrich Keilholz
Abstract Background Structured and harmonized implementation of molecular tumor boards (MTB) for the clinical interpretation of molecular data presents a current challenge for precision oncology. Heterogeneity in the interpretation of molecular data was shown for patients even with a limited number of molecular alterations. Integration of high-dimensional molecular data, including RNA- (RNA-Seq) and whole-exome sequencing (WES), is expected to further complicate clinical application. To analyze challenges for MTB harmonization based on complex molecular datasets, we...

Human resource needs and costs for HIV pre-exposure prophylaxis provision in nurse-led primary care in Eswatini and opportunities for task sharing

Stefan Kohler, Shona Dalal, Anita Hettema, Sindy Matse, Till Bärnighausen & Nicolas Paul
Abstract Background The global expansion of HIV pre-exposure prophylaxis (PrEP) includes health systems that face a shortage of skilled health care workers (HCWs). We estimated the human resource needs and costs for providing PrEP in nurse-led primary care clinics in Eswatini. Furthermore, we assessed potential cost savings from task sharing between nurses and other HCW cadres. Methods We conducted a time-and-motion and costing study in a PrEP demonstration project between August 2017 and January 2019....

Participatory disease surveillance for a mass gathering — a prospective cohort study on COVID-19, Germany 2021

Nils Hohmuth, Ifrah Khanyaree, Anna-Lena Lang, Ohad Duering, Stefan Konigorski, Vukašin Višković, Tobias Heising, Friedemann Egender, Cornelius Remschmidt & Rasmus Leistner
Abstract Background Mass gatherings (MGs) such as music festivals and sports events have been associated with a high risk of SARS-CoV-2 transmission. On-site research can foster knowledge of risk factors for infections and improve risk assessments and precautionary measures at future MGs. We tested a web-based participatory disease surveillance tool to detect COVID-19 infections at and after an outdoor MG by collecting self-reported COVID-19 symptoms and tests. Methods We conducted a digital prospective observational cohort...

Mobilisation of critically ill patients receiving norepinephrine: a retrospective cohort study

Maximilian Lindholz, Clara M. Schellenberg, Julius J. Grunow, Simone Kagerbauer, Annette Milnik, Daniel Zickler, Stefan Angermair, Anett Reißhauer, Martin Witzenrath, Mario Menk, Sebastian Boie, Felix Balzer & Stefan J. Schaller
Abstract Background Mobilisation and exercise intervention in general are safe and feasible in critically ill patients. For patients requiring catecholamines, however, doses of norepinephrine safe for mobilisation in the intensive care unit (ICU) are not defined. This study aimed to describe mobilisation practice in our hospital and identify doses of norepinephrine that allowed a safe mobilisation. Methods We conducted a retrospective single-centre cohort study of 16 ICUs at a university hospital in Germany with patients...

MEDICC2: whole-genome doubling aware copy-number phylogenies for cancer evolution

Tom L. Kaufmann, Marina Petkovic, Thomas B. K. Watkins, Emma C. Colliver, Sofya Laskina, Nisha Thapa, Darlan C. Minussi, Nicholas Navin, Charles Swanton, Peter Van Loo, Kerstin Haase, Maxime Tarabichi & Roland F. Schwarz
Abstract Aneuploidy, chromosomal instability, somatic copy-number alterations, and whole-genome doubling (WGD) play key roles in cancer evolution and provide information for the complex task of phylogenetic inference. We present MEDICC2, a method for inferring evolutionary trees and WGD using haplotype-specific somatic copy-number alterations from single-cell or bulk data. MEDICC2 eschews simplifications such as the infinite sites assumption, allowing multiple mutations and parallel evolution, and does not treat adjacent loci as independent, allowing overlapping copy-number events....

Characterization of key aroma compounds and regulation mechanism of aroma formation in local Binzi (Malus pumila × Malus asiatica) fruit

Qinghua Wang, Fan Gao, Xuexue Chen, Wenjiang Wu, Lei Wang, Jiangli Shi, Yun Huang, Yuanyue Shen, Guoliang Wu & Jiaxuan Guo
Abstract Background Volatile components are important secondary metabolites essential to fruit aroma quality, thus, in the past decades many studies have been extensively performed in clarifying fruit aroma formation. However, aroma components and biosynthesis in the fruit of Binzi (Malus pumila × Malus asiatica), an old local species with attractive aroma remain unknown. Results We investigated two Binzi cultivars, ‘Xiangbinzi’ (here named high-fragrant Binzi, ‘HFBZ’) and ‘Hulabin’ (here named low-fragrant Binzi, ‘LFBZ’) by monitoring the...

Extracellular matrix stiffness mediates radiosensitivity in a 3D nasopharyngeal carcinoma model

Yanhua Fang, Shanshan Liang, Jianong Gao, Zhe Wang, Cheng Li, Ruoyu Wang & Weiting Yu
Abstract Purpose Radiotherapy is one of the essential treatment modalities for nasopharyngeal carcinoma (NPC), however, radioresistance still poses challenges. Three-dimensional (3D) tumor culture models mimic the in vivo growth conditions of cells more accurately than 2D models. This study is to compare the tumor biological behaviors of NPC cells in 2D, On-Surface 3D and Embedded 3D systems, and to investigate the correlation between radioresistance and extracellular matrix (ECM) stiffness. Methods The morphology and radioresistance of...

Ability of patients with acute ischemic stroke to recall given information on intravenous thrombolysis: Results of a prospective multicenter study

Luzie Schuster, Fabian Essig, Naeimeh Daneshkhah, Juliane Herm, Simon Hellwig, Matthias Endres, Ulrich Dirnagl, Frank Hoffmann, Dominik Michalski, Waltraud Pfeilschifter, Christian Urbanek, Gabor C Petzold, Timolaos Rizos, Andrea Kraft & Karl Georg Haeusler
Introduction:Intravenous thrombolysis (IVT) is an on label treatment for selected patients with acute ischemic stroke (AIS). As major bleeding or allergic shock may occur, the need to ensure patients’ informed consent for IVT is a matter of debate.Patients and methods:Prospective investigator-initiated multi-center observational study to assess the ability of AIS patients to recall information, provided by a physician during a standardized educational talk (SET) on IVT use. The recall of 20 pre-defined items was assessed...

Macroscopic laser pulling based on the Knudsen force in rarefied gas

Lei Wang, Shige Wang, Qiuling Zhao & Xia Wang
Optical pulling is an attractive concept due to the counterintuitive feature, the profound mechanism underneath and promising applications. In recent ten years, optical pulling of micro-nano objects have been fully demonstrated. However, optical pulling of a macroscopic object is challenging. Herein, laser pulling of a macroscopic object is presented in rarefied gas. The pulling force is originated from the Kundsen force when a gauss laser beam irradiates a macroscopic structure composed of the absorptive bulk...

Registration Year

  • 2023
  • 2022

Resource Types

  • Collection


  • Charité - University Medicine Berlin
  • Sichuan University
  • Fudan University
  • Shanghai Jiao Tong University
  • Zhejiang University
  • Jiangsu Province Hospital
  • Chinese Academy of Sciences
  • Sun Yat-sen University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Sun Yat-sen University Cancer Center