BoxCARS and pump-probe geometries are common implementations of two dimensional infrared (2D IR) spectroscopy. BoxCARS is background-free, generally offering greater signal to noise ratio, which enables measuring weak vibrational echo signals. Pulse shapers have been implemented in the pump probe geometry to accelerate data collection and suppress scatter and other unwanted signals by precise control of the pump pulse delay and carrier phase. Here, we introduce a 2D IR optical setup in the BoxCARS geometry...

Rapid coherent Raman hyperspectral imaging shows great promise for applications in sensing, medical diagnostics and metabolism dynamic monitoring. However, the spectral acquisition speed of current multiplex CARS microscopy is generally limited by the spectrometer integration time, making it difficult to achieve the video frame rate imaging. On the other hand, as the detection speed increases, the signal-to-noise ratio (SNR) of single spectrum will decrease leading to a terrible imaging quality. In this letter, we report...

Rapid coherent Raman hyperspectral imaging shows great promise for applications in sensing, medical diagnostics and metabolism dynamic monitoring. However, the spectral acquisition speed of current multiplex CARS microscopy is generally limited by the spectrometer integration time, making it difficult to achieve the video frame rate imaging. On the other hand, as the detection speed increases, the signal-to-noise ratio (SNR) of single spectrum will decrease leading to a terrible imaging quality. In this letter, we report...

Optical pulling is an attractive concept due to the counterintuitive feature, the profound mechanism underneath and promising applications. In recent ten years, optical pulling of micro-nano objects have been fully demonstrated. However, optical pulling of a macroscopic object is challenging. Herein, laser pulling of a macroscopic object is presented in rarefied gas. The pulling force is originated from the Kundsen force when a gauss laser beam irradiates a macroscopic structure composed of the absorptive bulk...

Optical waveguide amplifiers are essential devices in integrated optical systems. Their gain bandwidths directly determine the operating wavelength of optical circuits. Due to the difficulty of developing wideband gain media, it has been a challenge to fabricate devices with broadband amplification capability, resulting in few reports on multi-band polymer waveguide amplifiers. Here, a polymer waveguide amplifier is demonstrated, which achieves loss compensation covering the whole (S+C)-band by using NaYF4:Tm,Yb@NaYF4@NaYF4:Er nanoparticles-doped SU-8 as the gain medium....

Multimode fibers (MMFs) are emerging as promising transmission media for delivering images. However, strong mode coupling inherent in MMFs induces difficulties in directly projecting two-dimensional images through MMFs. By training two subnetworks named Actor-net and Model-net synergetically, Ref. [1] alleviated this issue and demonstrated projecting images through MMFs with high fidelity. In this work, we make a step further by improving the generalization ability to greyscale images. The modified projector network contains three subnetworks, namely...

Abstract Background Elucidating the phylogenetic relationships within species-rich genera is essential but challenging, especially when lineages are assumed to have been going through radiation events. Mahonia Nutt. (Berberidaceae) is a genus with cosmopolitan distribution, comprising approximately 100 species, two of which are known as Caulis Mahoniae (M. bealei and M. fortunei) with crucial pharmacological significance in Chinese herbal medicine. Mahonia is a taxonomically challenging genus, and intrageneric phylogenetic relationships still need to be explored using...

Abstract Clear cell renal cell carcinoma (ccRCC) is a common malignant tumor of the urinary system characterized by poor prognosis and difficult treatment. It has been reported that iron metabolism dysregulation is a common phenomenon in ccRCC and is closely related to the process of ccRCC. But still now, the exact function and underlying mechanisms of iron metabolism dysregulation in ccRCC have not been fully elucidated. In this study, we comprehensively investigated the prognostic value...

Abstract Background There is a raising concern of a higher infectious Omicron BA.2 variant and the latest BA.4, BA.5 variant, made it more difficult in the mitigation process against COVID-19 pandemic. Our study aimed to find optimal control strategies by transmission of dynamic model from novel invasion theory. Methods Based on the public data sources from January 31 to May 31, 2022, in four cities (Nanjing, Shanghai, Shenzhen and Suzhou) of China. We segmented the...

Abstract Background There is a raising concern of a higher infectious Omicron BA.2 variant and the latest BA.4, BA.5 variant, made it more difficult in the mitigation process against COVID-19 pandemic. Our study aimed to find optimal control strategies by transmission of dynamic model from novel invasion theory. Methods Based on the public data sources from January 31 to May 31, 2022, in four cities (Nanjing, Shanghai, Shenzhen and Suzhou) of China. We segmented the...

Abstract Introduction Accumulating evidence highlights the critical roles of circular RNAs (circRNAs) in the malignant progression of cancers. In this study, we investigated the expression pattern of a newly identified circRNA (hsa_circ_0017109) in non–small cell lung cancer (NSCLC), and examined its downstream molecular targets. Methods Quantitative real-time PCR (qRT-PCR) and Western blotting (WB) were conducted to quantify gene and protein expression. In vitro functional assays such as colony formation assay, cell counting kit-8 (CCK-8) and...

In this work, we proposed an optical trapping and manipulation technology based on spatial diffraction of 45° tilted fiber Bragg grating (TFBG). The length of the line-shape-facula of the TFBG diffraction light can be as large as tens of millimeters, which enables the TFBG trapping system control massive dielectric particles. We analyze the light distribution of the spatial diffraction by using the volume current method (VCM) and established a theoretical model to analyze the optical...

Abstract Background Hepatocellular carcinoma (HCC) is one of the deadliest cancers and is mainly developed from chronic liver diseases such as hepatitis-B infection-associated liver cirrhosis (LC). The progression from LC to HCC makes the detection of diagnostic biomarkers to be challenging. Hence, there have been constant efforts to improve on identifying the critical and predictive changes accompanying the disease progression. Methods In this study, we looked to using the mass spectrometry mediated spatial metabolomics technique...

Abstract Objective Hyperhomocysteinemia (HHcy) is considered to increase the risk of sarcopenia (S) and remains controversial. In this study, we aimed to investigate the prevalence of S among older Chinese adults and explore whether homocysteine (Hcy) was independently associated with S. Methods This cross-sectional study was performed among older adults hospitalized in the Geriatric Hospital of Nanjing Medical University between June 2017 and December 2021. We measured all participants’ serum Hcy levels, hand grip strength,...

Abstract Background Previous studies have reported that maternal smoking during pregnancy and breastfeeding may affect the occurrence of hypertension, but whether early life factors modify the impact of the offspring’s genetic risk on hypertension is still unknown. The aim of this study was to investigate the relationships among maternal smoking and breastfeeding with adult-onset hypertension and the modified impact of offspring genetic susceptibility. Methods This study included 437,185 participants from the UK Biobank who were...

Objective: Polyneuropathy, organomegaly, endocrinopathy, M-protein, skin changes (POEMS) syndrome is a rare monoclonal plasma cell proliferation disorder. At present, there is no unified treatment for POEMS syndrome. Here, we describe one case with POEMS syndrome with obvious neurological symptoms diagnosed in September 2020. We made a meta-­analysis to assess the efficacy of treatment strategies in recent years. Methods: We retrospectively analyzed the diagnosis and treatment of this patient, and searched relevant articles in PubMed, Embase,...

Sumatra is located at the western end of the Sunda Arc, which resulted from the subduction of the Indo-Australian Plate beneath the Eurasian Plate. In this study, we report detailed zircon U–Pb and Hf isotope data for Cenozoic igneous rocks from the entire island of Sumatra to better constrain the temporal and spatial distribution of arc magmatism. The new dataset, combined with literature information, identifies the following two magmatic stages: (1) Paleocene to Early Eocene...

Organoids, the 3D culture systems derived from stem cells, are promising models for human organs. But organoid study requires large-volume imaging with single cell resolution, which is beyond the spatial bandwidth limit of conventional optical microscopy. Herein, we propose a lensless holographic microscope empowered with a time and memory-saving algorithm. It solves the tradeoff between the imaging field of view, resolution and processing speed, and provides a practical tools for organoids’ study. We first build...

Learning-based computer-generated holography (CGH) algorithms appear as novel alternatives to generate phase-only holograms. However, most existing learning-based approaches underperform their iterative peers in terms of display quality. Here, we propose an unsupervised Fourier-inspired neural network that could generate phase-only holograms in real time and obtain high-fidelity reconstructed images. Both simulation and experiment were performed to showcase its capability. By explicitly leveraging Fourier transforms within the neural network architecture, global features of the holograms are more...

Abstract Background N6-methyladenosine (m6A) related long noncoding RNAs (lncRNAs) may have prognostic value in bladder cancer for their key role in tumorigenesis and innate immunity. Methods Bladder cancer transcriptome data and the corresponding clinical data were acquired from the Cancer Genome Atlas (TCGA) database. The m6A-immune-related lncRNAs were identified using univariate Cox regression analysis and Pearson correlation analysis. A risk model was established using least absolute shrinkage and selection operator (LASSO) Cox regression analyses, and...

Abstract Background In recent years, it has been proved that necroptosis plays an important role in the occurrence, development, invasion, metastasis and drug resistance of malignant tumors. Hence, further evaluation and targeting of necroptosis may be of clinical benefit for gynecologic cancers (GCs). Methods To compare consistency and difference, we explored the expression pattern and prognostic value of necroptosis-related genes (NRGs) in pan-GC analysis through Linear regression and Empirical Bayesian, Univariate Cox analysis, and public...

Purpose:To investigate the demographics, clinical features, radiologic measurement, treatment, and outcomes of symptomatic spontaneous isolated superior mesenteric artery dissection (SISMAD) according to computed tomography (CT) classification.Methods:This retrospective study included 201 patients diagnosed with symptomatic SISMAD from November 2014 to December 2020. Symptomatic spontaneous isolated superior mesenteric artery dissection was categorized into four types based on CT images by Yun’s angiographic classification. Their clinical characteristics, images features, treatment methods, and radiological outcomes were comparatively analyzed by...

Abstract Background The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the cause of coronavirus disease 2019 (COVID-19), which is currently a worldwide pandemic. There are limited available treatments for severe COVID-19 patients. However, some evidence suggests that intravenous immunoglobulin (IVIg) provides clinical benefits for these patients. Methods We administered IVIg to 23 severe COVID-19 patients, and all of them survived. Four related coronaviruses can cause the common cold. We speculated that cross-reactivity of SARS-CoV-2 and...

Abstract Background The molecular mechanism of laryngeal squamous cell carcinoma (LSCC) is not completely clear, which leads to poor prognosis and treatment difficulties for LSCC patients. To date, no study has reported the exact expression level of zinc finger protein 71 (ZNF71) and its molecular mechanism in LSCC. Methods In-house immunohistochemistry (IHC) staining (33 LSCC samples and 29 non-LSCC samples) was utilized in analyzing the protein expression level of ZNF71 in LSCC. Gene chips and...

Abstract Background MICAL1 is involved in the malignant processes of several types of cancer; however, the role of MICAL1 in pancreatic cancer (PC) has not been well-characterized. This study aimed to investigate the expression and function of MICAL1 in PC. Methods RT-qPCR and immunohistochemistry were used to detect MICAL1 expression in PC and adjacent nontumor tissues. Cell Counting Kit-8, EdU, clone formation, wound healing, and Transwell assays as well as animal models were used to...