10 Works

Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia

Ursula Panzner, Ondari Daniel Mogeni, Yaw Adu-Sarkodie, Trevor Toy, Hyon Jin Jeon, Gi Deok Pak, Se Eun Park, Yeetey Enuameh, Ellis Owusu-Dabo, Trinh Van Tan, Abraham Aseffa, Mekonnen Teferi, Biruk Yeshitela, Stephen Baker, Raphael Rakotozandrindrainy & Florian Marks
Abstract Background We aimed to assess the prevalence of Salmonella Typhi through DNA and IgM-antibody detection methods as a prelude to extended surveillance activities at sites in Ghana, Madagascar, and Ethiopia. Methods We performed species-specific real-time polymerase reaction (RT-PCR) to identify bacterial nucleic acid, and enzyme-linked immunosorbent assay (ELISA) for detecting HlyE/STY1498-, CdtB/STY1886-, pilL/STY4539- and Vi-antigens in blood and biopsy specimens of febrile and non-febrile subjects. We generated antigen-specific ELISA proxy cut-offs by change-point analyses,...

Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia

Ursula Panzner, Ondari Daniel Mogeni, Yaw Adu-Sarkodie, Trevor Toy, Hyon Jin Jeon, Gi Deok Pak, Se Eun Park, Yeetey Enuameh, Ellis Owusu-Dabo, Trinh Van Tan, Abraham Aseffa, Mekonnen Teferi, Biruk Yeshitela, Stephen Baker, Raphael Rakotozandrindrainy & Florian Marks
Abstract Background We aimed to assess the prevalence of Salmonella Typhi through DNA and IgM-antibody detection methods as a prelude to extended surveillance activities at sites in Ghana, Madagascar, and Ethiopia. Methods We performed species-specific real-time polymerase reaction (RT-PCR) to identify bacterial nucleic acid, and enzyme-linked immunosorbent assay (ELISA) for detecting HlyE/STY1498-, CdtB/STY1886-, pilL/STY4539- and Vi-antigens in blood and biopsy specimens of febrile and non-febrile subjects. We generated antigen-specific ELISA proxy cut-offs by change-point analyses,...

Effectiveness of school-based interventions for preventing tobacco smoking initiation among young people in low- and middle-income countries: a systematic review protocol

Divine Darlington Logo, Yeetey Enuameh, George Adjei, Arti Singh, Emmanuel Nakua, Edward Dassah, Felix Boakye Oppong & Ellis Owusu-Dabo
Abstract Background Despite the commendable progress made globally in tobacco control, the world is falling short of achieving a 30% relative reduction in current tobacco use by 2025. The African region remains the least in the efforts in fighting the tobacco epidemic and is most exploited by the tobacco industry. Schools have been continuously used for over three decades as a setting for delivering youth smoking prevention programmes; however, the evidence of the effectiveness of...

Damaging Mutations in AFDN Contribute to Risk of Nonsyndromic Cleft Lip With or Without Cleft Palate

Waheed Awotoye, Peter A Mossey, Jacqueline B Hetmanski, Lord J J Gowans, Mekonen A Eshete, Wasiu L Adeyemo, Azeez Alade, Erliang Zeng, Olawale Adamson, Olutayo James, Azeez Fashina, Modupe O Ogunlewe, Thirona Naicker, Chinyere Adeleke, Tamara Busch, Mary Li, Aline Petrin, Abimbola Oladayo, Sami Kayali, Joy Olotu, Veronica Sule, Mohaned Hassan, John Pape, Emmanuel T Aladenika, Peter Donkor … & Azeez Butali
ObjectivesNovel or rare damaging mutations have been implicated in the developmental pathogenesis of nonsyndromic cleft lip with or without cleft palate (nsCL ± P). Thus, we investigated the human genome for high-impact mutations that could explain the risk of nsCL ± P in our cohorts.Materials and Methods:We conducted next-generation sequencing (NGS) analysis of 130 nsCL ± P case-parent African trios to identify pathogenic variants that contribute to the risk of clefting. We replicated this analysis...

Damaging Mutations in AFDN Contribute to Risk of Nonsyndromic Cleft Lip With or Without Cleft Palate

Waheed Awotoye, Peter A Mossey, Jacqueline B Hetmanski, Lord J J Gowans, Mekonen A Eshete, Wasiu L Adeyemo, Azeez Alade, Erliang Zeng, Olawale Adamson, Olutayo James, Azeez Fashina, Modupe O Ogunlewe, Thirona Naicker, Chinyere Adeleke, Tamara Busch, Mary Li, Aline Petrin, Abimbola Oladayo, Sami Kayali, Joy Olotu, Veronica Sule, Mohaned Hassan, John Pape, Emmanuel T Aladenika, Peter Donkor … & Azeez Butali
ObjectivesNovel or rare damaging mutations have been implicated in the developmental pathogenesis of nonsyndromic cleft lip with or without cleft palate (nsCL ± P). Thus, we investigated the human genome for high-impact mutations that could explain the risk of nsCL ± P in our cohorts.Materials and Methods:We conducted next-generation sequencing (NGS) analysis of 130 nsCL ± P case-parent African trios to identify pathogenic variants that contribute to the risk of clefting. We replicated this analysis...

The incidence of TB and MDR-TB in pediatrics and therapeutic options: a systematic review

Sheetal Harichander, Ebenezer Wiafe, Kofi Boamah Mensah, Varsha Bangalee & Frasia Oosthuizen
Abstract Background Tuberculosis (TB) is considered one of the top 10 causes of death worldwide and the leading cause of death from a single infectious agent. Multidrug-resistant (MDR) TB can affect people of all age groups, including children (aged 0–15 years). However, very little is known about the extent of this problem in children. This systematic review aims to investigate the incidence of TB and drug-resistant (DR) TB among the pediatric population. It also reviews...

The incidence of TB and MDR-TB in pediatrics and therapeutic options: a systematic review

Sheetal Harichander, Ebenezer Wiafe, Kofi Boamah Mensah, Varsha Bangalee & Frasia Oosthuizen
Abstract Background Tuberculosis (TB) is considered one of the top 10 causes of death worldwide and the leading cause of death from a single infectious agent. Multidrug-resistant (MDR) TB can affect people of all age groups, including children (aged 0–15 years). However, very little is known about the extent of this problem in children. This systematic review aims to investigate the incidence of TB and drug-resistant (DR) TB among the pediatric population. It also reviews...

Multi-drug resistant bacteria isolates from lymphatic filariasis patients in the Ahanta West District, Ghana

Bill Clinton Aglomasa, Cynthia Kyerewaa Adu-Asiamah, Samuel Opoku Asiedu, Priscilla Kini, Emmanuel Kobla Atsu Amewu, Kennedy Gyau Boahen, Solomon Wireko, Isaac Kingsley Amponsah, Yaw Duah Boakye, Vivian Etsiapa Boamah & Alexander Kwarteng
Abstract Background Antimicrobial resistance is associated with increased morbidity in secondary infections and is a global threat owning to the ubiquitous nature of resistance genes in the environment. Recent estimate put the deaths associated with bacterial antimicrobial resistance in 2019 at 4.95 million worldwide. Lymphatic filariasis (LF), a Neglected Tropical Disease (NTD), is associated with the poor living in the tropical regions of the world. LF patients are prone to developing acute dermatolymphangioadenitis (ADLA), a...

Multi-drug resistant bacteria isolates from lymphatic filariasis patients in the Ahanta West District, Ghana

Bill Clinton Aglomasa, Cynthia Kyerewaa Adu-Asiamah, Samuel Opoku Asiedu, Priscilla Kini, Emmanuel Kobla Atsu Amewu, Kennedy Gyau Boahen, Solomon Wireko, Isaac Kingsley Amponsah, Yaw Duah Boakye, Vivian Etsiapa Boamah & Alexander Kwarteng
Abstract Background Antimicrobial resistance is associated with increased morbidity in secondary infections and is a global threat owning to the ubiquitous nature of resistance genes in the environment. Recent estimate put the deaths associated with bacterial antimicrobial resistance in 2019 at 4.95 million worldwide. Lymphatic filariasis (LF), a Neglected Tropical Disease (NTD), is associated with the poor living in the tropical regions of the world. LF patients are prone to developing acute dermatolymphangioadenitis (ADLA), a...

Effectiveness of school-based interventions for preventing tobacco smoking initiation among young people in low- and middle-income countries: a systematic review protocol

Divine Darlington Logo, Yeetey Enuameh, George Adjei, Arti Singh, Emmanuel Nakua, Edward Dassah, Felix Boakye Oppong & Ellis Owusu-Dabo
Abstract Background Despite the commendable progress made globally in tobacco control, the world is falling short of achieving a 30% relative reduction in current tobacco use by 2025. The African region remains the least in the efforts in fighting the tobacco epidemic and is most exploited by the tobacco industry. Schools have been continuously used for over three decades as a setting for delivering youth smoking prevention programmes; however, the evidence of the effectiveness of...

Registration Year

  • 2022
    10

Resource Types

  • Collection
    10

Affiliations

  • Kwame Nkrumah University of Science and Technology
    10
  • Kintampo Health Research Centre
    4
  • Kumasi Technical University
    2
  • Johns Hopkins University
    2
  • Kangbuk Samsung Hospital
    2
  • University of Cambridge
    2
  • Federal Ministry of Health
    2
  • World Health Organization
    2
  • University of Basel
    2
  • University of Antananarivo
    2