266 Works

Mucosal-associated invariant T cells predict increased acute graft-versus-host-disease incidence in patients receiving allogeneic hematopoietic stem cell transplantation

Zhao Wang, Sudong Zhang, Xiaoyu Zhang, Li Liu, Lukun Zhou, Yuyan Shen, Rongli Zhang, Yi He, Donglin Yang, Erlie Jiang, Xiaoming Feng, Jiaxi Zhou, Tao Cheng, Mingzhe Han & Sizhou Feng
Abstract Background Mucosal-associated invariant T (MAIT) cells are innate-like T cells, some studies have reported that the number of circulating MAIT cells reduced in patients with acute graft-versus-host-disease (aGVHD) development. However, the role of donor MAIT cells on aGVHD development and subsequent functional change still remain unclear. Methods The study recruited 86 patients with hematological malignancies who underwent allogeneic hematopoietic cell transplantation (HCT) from May 1, 2018 to June 30, 2019. MAIT cells, their subset,...

Influence of the graft-copolymerized hydrophilic lignin content on the structure and mechanical properties of gel-spun poly(vinyl alcohol) composite fibers

Yu Cheng, Jiaxian Lin, Xiaorui Sun, Lianjie Duan, Yuanyuan Zheng, Xiaoxia Sun, Xian Li & Chunhong Lu
Lignin is a cost-effective, biobased filler for the fabrication of high-performance poly(vinyl alcohol) (PVA) composite fibers that increases fiber performance and sustainability. However, amorphous and hydrophobic lignin often have poor compatibility with semi-crystalline and hydrophilic PVA matrixes. Moreover, aggregation of the filler at high content could occur to impede the effectiveness of lignin as a reinforcing filler. To address these issues, modified organosolv lignin (OL24) with hydrophilicity was obtained from the graft copolymerization of organosolv...

Supplementary material from \"Hoxa9/meis1-transgenic zebrafish develops acute myeloid leukaemia-like disease with rapid onset and high penetrance\"

Wei Wang, Hongji Li, Mengling Huang, Xue Wang, Wei Li, Xiaoqing Qian & Lili Jing
HOXA9 and MEIS1 are co-expressed in over 50% of acute myeloid leukaemia (AML) and play essential roles in leukemogenesis, but the mechanisms involved are poorly understood. Diverse animal models offer valuable tools to recapitulate different aspects of AML and link in vitro studies to clinical trials. We generated a double transgenic zebrafish that enables hoxa9 overexpression in blood cells under the draculin (drl) regulatory element and an inducible expression of meis1 through a heat shock...

Failure pattern and suggestions for target volume delineation of carcinoma showing thymus-like differentiation treated with intensity-modulated radiotherapy

Fang-Fang Kong, Guang-Sen Pan, Rui-Ping Zhai, Cheng-Run Du, Xia-Yun He, Chun-Ying Shen, Xue-Guan Lu, Tuan-Qi Sun, Yu Wang, Qing-Hai Ji, Chao-Su Hu & Hong-Mei Ying
Abstract Background To review our long-term clinical experience, analyze the failure patterns, and give suggestions for target volume delineation of carcinoma showing thymus-like differentiation (CASTLE) treated with intensity-modulated radiotherapy (IMRT). Methods From April 2008 to May 2019, 30 patients with CASTLE treated by postoperative or radical IMRT in our center were retrospectively reviewed. A total dose of 56-60 Gy in 28–30 fractions was prescribed to patients without residual disease and 66 Gy in 33 fractions...

Implantation of adipose-derived mesenchymal stem cell sheets promotes axonal regeneration and restores bladder function after spinal cord injury

Jiasheng Chen, Lin Wang, Meng Liu, Guo Gao, Weixin Zhao, Qiang Fu & Ying Wang
Abstract Background Cell-based therapy using adipose-derived mesenchymal stem cells (ADSCs) is a promising treatment strategy for neurogenic bladder (NB) associated with spinal cord injury (SCI). However, therapeutic efficacy is low because of inefficient cell delivery. Cell sheets improve the efficacy of cell transplantation. Therefore, this study was conducted to investigate the therapeutic efficacy of transplanting ADSC sheets into an SCI rat model and focused on the function and pathological changes of the bladder. Methods ADSC...

Toward efficient and high-fidelity metagenomic data from sub-nanogram DNA: evaluation of library preparation and decontamination methods

Chun Wang, Li Zhang, Xuan Jiang, Wentai Ma, Hui Geng, Xue Wang & Mingkun Li
Abstract Background Shotgun metagenomic sequencing has greatly expanded the understanding of microbial communities in various biological niches. However, it is still challenging to efficiently convert sub-nanogram DNA to high-quality metagenomic libraries and obtain high-fidelity data, hindering the exploration of niches with low microbial biomass. Results To cope with this challenge comprehensively, we evaluated the performance of various library preparation methods on 0.5 pg–5 ng synthetic microbial community DNA, characterized contaminants, and further applied different in...

MICALL2 as a substrate of ubiquitinase TRIM21 regulates tumorigenesis of colorectal cancer

Pushuai Wen, Huade Wang, Yi Li, Xinyao Sui, Zhijuan Hou, Xiaoyan Guo, Wanying Xue, Dahua Liu, Yu Wang & Jing Gao
Abstract Background Molecule interacting with CasL-like protein 2 (MICALL2) is believed to regulate cytoskeleton dynamics, tight junction formation, and neurite outgrowth. However, its biological role and the underlying mechanism in colorectal cancer (CRC) remain largely elusive. Methods qRT-PCR, Western blotting and immunohistochemistry assays were used to detect the expression levels of different genes. Next, mass spectrometry, co-immunoprecipitation and immunofluorescence staining were used to detect the interactions of proteins. Furthermore, MTT assay, colony formation assay, wound-healing...

Nasopharyngeal aspirates in children with severe community-acquired pneumonia collected within 3 days before bronchoscopy can partially reflect the pathogens in bronchoalveolar lavage fluids

Qiguo Zhu, Junli Zhou, Fei Li, Peng Shi, Yi Lu, Xiaoliang Lin, Lin Yuan, Zhiqiang Zhuo & Jun Shen
Abstract Background There is little evidence about consistency between nasopharyngeal and pulmonary pathogens in children with severe pneumonia. This study aims to compare the difference of pathogens between nasopharyngeal aspirates (NPAs) collected before bronchoscopy and bronchoalveolar lavage fluids (BALFs) in children with severe community-acquired pneumonia (SCAP). Methods NPAs and BALFs were collected form pediatric SCAP cases hospitalized from January 2018 to March 2019. NPAs were colleced within 3 days before bronchoscopy. Samples were detected by...

Gender-specific relationship between frequency of food-away-from-home with serum lipid levels and dyslipidemia in chinese rural adults

Yuyang Wang, Xiaotian Liu, Xiaokang Dong, Beibei Liu, Ning Kang, Wenqian Huo, Zhenxing Mao, Jian Hou & Chongjian Wang
Abstract Objective Food-away-from-home (FAFH) is one of the leading dietary patterns in Chinese families. However, the relationship between FAFH and dyslipidemia remains unclear, especially in the rural adult population. This study explored the relationship of FAFH frequency with serum lipid levels and dyslipidemia in rural Chinese adults. Methods A total of 12,002 men and 17,477 women aged 18–79 were included from the Henan rural cohort. Serum lipid levels were measured by enzyme colorimetry. Information on...

A machine learning-based data mining in medical examination data: a biological features-based biological age prediction model

Qing Yang, Sunan Gao, Junfen Lin, Ke Lyu, Zexu Wu, Yuhao Chen, Yinwei Qiu, Yanrong Zhao, Wei Wang, Tianxiang Lin, Huiyun Pan & Ming Chen
Abstract Background Biological age (BA) has been recognized as a more accurate indicator of aging than chronological age (CA). However, the current limitations include: insufficient attention to the incompleteness of medical data for constructing BA; Lack of machine learning-based BA (ML-BA) on the Chinese population; Neglect of the influence of model overfitting degree on the stability of the association results. Methods and results Based on the medical examination data of the Chinese population (45–90 years),...

Age- and sex-related differences in the retinal capillary plexus in healthy Chinese adults

Binbin Su, Xiaoxuan Zhu, Kai Yang, Yunfan Xiao, Chunmei Li, Keai Shi, Jia Qu, Fan Lu, Ming Li & Lele Cui
Abstract Background To assess age- and sex-related changes in the superficial retinal capillary plexus (SCP) and deep retinal capillary plexus (DCP) in healthy Chinese adults. Methods In this cross-sectional study, all data were derived from the community-based Jidong Eye Cohort Study. Participants underwent optical coherence tomography angiography (OCTA) and other ocular and systemic examinations. The vessel densities of the whole measured area, parafovea, and four quadrants in the SCP and DCP were measured. Results We...

YAP1 protects against septic liver injury via ferroptosis resistance

Jin Wang, Qian Zhu, Rui Li, Jing Zhang, Xujun Ye & Xinyi Li
Abstract Background The liver plays crucial roles in sepsis and is one of the major targets for sepsis-related injuries. Ferroptosis, a newly emerged form of lytic cell death, has been implicated in sepsis related organ failure. Yes-associated protein1 (YAP1), a key regulator of the Hippo signaling pathway, may be involved in ferroptosis development. This study aimed to elucidate the role of YAP1 in septic liver injury through regulating ferroptosis, especially ferritinophagy-mediated ferroptosis. Results Cecal ligation...

Cancer-associated fibroblast-derived gene signatures predict radiotherapeutic survival in prostate cancer patients

Ran Zhang & Feng Liu
Abstract Background Cancer-associated fibroblasts (CAFs) play multiple roles in regulating tumor metastasis and treatment response. Current clinical indicators are insufficient to accurately assess disease risk and radiotherapy response, emphasizing the urgent need for additional molecular prognostic markers. Methods In order to investigate CAF-related genes associated with radiotherapy and construct prognostic CAF-related gene signatures for prostate cancer, we firstly established a radio-resistant prostate CAF cell subline (referred to as CAFR) from Mus-CAF (referred to as CAF)...

The Effect of Coumestrol on Hub Genes in Lung Squamous Cell Carcinoma Based on Bioinformatic Strategy

Qingqing Shan, Yifan Zhang, Xu Zhang, Wei Wang & Zongan Liang
Purpose There is limited treatment for lung squamous cell carcinoma (LUSC), so there is an urgent need to find new antitumor drugs. Materials and Methods We downloaded datasets from the Gene Expression Omnibus (GEO) and the Cancer Genome Atlas databases. We used GEO2R and the “limma” package to screen differentially expressed genes. We used the Cytoscape software to screen out hub genes. We screened herbs that act on hub genes on the Chinese medicine website....

Left ventricular non-compaction cardiomyopathy associated with the PRKAG2 mutation

Jing Zhang, Xiu Han, Qun Lu, Yunfei Feng, Aiqun Ma & Tingzhong Wang
Abstract Left ventricular non-compaction cardiomyopathy (LVNC) is one of the most common inherited cardiovascular diseases. The genetic backgrounds of most LVNC patients are not fully understood. We collected clinical data, family histories, and blood samples and performed genetic analysis using next-generation sequencing (NGS) from a Chinese family of 15 subjects. Clinically LVNC affected subjects showed marked cardiac phenotype heterogeneity. We found that these subjects with LVNC carried a missense heterozygous genetic mutation c.905G>A (p.R302Q) in...

Antibiofilm activity of ultra-small gold nanoclusters against Fusobacterium nucleatum in dental plaque biofilms

Yangheng Zhang, Rixin Chen, Yuxian Wang, Peng Wang, Jiajie Pu, Xiaoqiang Xu, Faming Chen, Ling Jiang, Qing Jiang & Fuhua Yan
Abstract Pathogenic dental plaque biofilms are universal and harmful, which can result in oral infections and systemic diseases. Many conventional therapeutic methods have proven insufficient or ineffective against plaque biofilms. Therefore, new strategies are urgently needed. Fusobacterium nucleatum (F. nucleatum), a periodontal pathogen associated with a variety of oral and systemic diseases, is thought to be central to the development and structure of dental plaques. Here, ultra-small gold nanoclusters (AuNCs) were prepared. They exhibited potent...

Genomic landscape, immune characteristics and prognostic mutation signature of cervical cancer in China

Jing Liu, Zirong Li, Ting Lu, Junping Pan, Li Li, Yanwen Song, Dan Hu, Yanhong Zhuo, Ying Chen & Qin Xu
Abstract Purpose This study aimed to analyse the genomic alteration profiles and immune characteristics of a cohort of Chinese cervical cancer patients to understand why certain patients benefited from molecular targeted therapies and immunotherapy as well as their prognostic significance. Methods PD-L1 expression and clinicopathological information were obtained from 98 cervical cancer patients. Differences in PD-L1 expression and gene mutations between squamous cell carcinoma (SCC) and adenocarcinoma (AC) were analysed by the chi-square test or...

Aligned nanofibrous collagen membranes from fish swim bladder as a tough and acid-resistant suture for pH-regulated stomach perforation and tendon rupture

Zhaohui Luan, Shuang Liu, Wei Wang, Kaige Xu, Shaosong Ye, Ruijue Dan, Hong Zhang, Zhenzhen Shu, Tongchuan Wang, Chaoqiang Fan, Malcolm Xing & Shiming Yang
Abstract Background Wound closure in the complex body environment places higher requirements on suture’s mechanical and biological performance. In the scenario of frequent mechanical gastric motility and extremely low pH, single functional sutures have limitations in dealing with stomach bleeding trauma where the normal healing will get deteriorated in acid. It necessitates to advance suture, which can regulate wounds, resist acid and intelligently sense stomach pH. Methods Based on fish swim bladder, a double-stranded drug-loaded...

Dementia risk prediction in individuals with mild cognitive impairment: a comparison of Cox regression and machine learning models

Meng Wang, Matthew Greenberg, Nils D. Forkert, Thierry Chekouo, Gabriel Afriyie, Zahinoor Ismail, Eric E. Smith & Tolulope T. Sajobi
Abstract Background Cox proportional hazards regression models and machine learning models are widely used for predicting the risk of dementia. Existing comparisons of these models have mostly been based on empirical datasets and have yielded mixed results. This study examines the accuracy of various machine learning and of the Cox regression models for predicting time-to-event outcomes using Monte Carlo simulation in people with mild cognitive impairment (MCI). Methods The predictive accuracy of nine time-to-event regression...

Mining and characterization of the PKS–NRPS hybrid for epicoccamide A: a mannosylated tetramate derivative from Epicoccum sp. CPCC 400996

Tao Zhang, Guowei Cai, Xiaoting Rong, Jingwen Xu, Bingya Jiang, Hao Wang, Xinxin Li, Lu Wang, Ran Zhang, Wenni He & Liyan Yu
Abstract Background Genomic analysis indicated that the genomes of ascomycetes might carry dozens of biosynthetic gene clusters (BGCs), yet many clusters have remained enigmatic. The ascomycete genus Epicoccum, belonging to the family Didymellaceae, is ubiquitous that colonizes different types of substrates and is associated with phyllosphere or decaying vegetation. Species of this genus are prolific producers of bioactive substances. The epicoccamides, as biosynthetically distinct mannosylated tetramate, were first isolated in 2003 from Epicoccum sp. In...

A Meta-Research of Randomized Controlled Trials in the Field of Mental Health: Comparing Pharmacological to Non-Pharmacological Interventions from 1955 to 2020

Sheng Chen, Alina Lee & Wei Wang
ObjectiveThis study aimed to provide a general overview of mental health randomized controlled trials (RCTs) and summarize the temporal trends in terms of the number of studies, median sample sizes, and median effect sizes using data collected from the Cochrane Database of Systematic Reviews (CDSR).MethodsUsing data collected from the CDSR, the temporal trends are compared in terms of the number of studies, median sample sizes, and median effect sizes between two broad categories of interventions:...

Genetic and Clinical Characteristics of Patients with Philadelphia-Negative Myeloproliferative Neoplasm Carrying Concurrent Mutations in JAK2V617F, CALR, and MPL

Yan Wang, Fei Ran, Jin Lin, Jing Zhang & Dan Ma
Simultaneous mutations in Janus kinase 2 (JAK2), calreticulin, and myeloproliferative leukemia (MPL) genes are generally not considered for characterizing Philadelphia-negative myeloproliferative neoplasms (MPNs), leading to misdiagnosis. Sanger sequencing and quantitative polymerase chain reaction were used to detect gene mutations in patients with MPN. We retrospectively screened the data of patients with double mutations in our center and from the PubMed database. Two patients tested positive for both JAK2V617F and CALR mutations (2/352 0.57%) in our...

Supplementary material from \"Spatial transcriptomics and microbiota reveal immune mechanism that response to pathogen infection in the posterior intestine of Sebastes schlegelii\"

Min Cao, Ting Xue, Huijun Huo, Xiaoyan Zhang, Ning ning Wang, Xu Yan & Chao Li
Intestine is a place that a site of immune cells priming at birth. Therefore, spatial transcriptomics was performed to define how the transcriptomic landscape is spatially organized in the posterior intestine of Sebastes schlegelii following Edwardsiella piscicida infection. At the healthy condition, we identified a previously unappreciated molecular regionalization of the posterior intestine. Following bacterial infection, most immune-related genes were identified in mucosa layers. Moreover, investigation of immune-related genes and genes in immune-related KEGG pathways...

Deep brain stimulation of the nucleus basalis of Meynert modulates hippocampal–frontoparietal networks in patients with advanced Alzheimer’s disease

Yin Jiang, Tian-Shuo Yuan, Ying-Chuan Chen, Peng Guo, Teng-Hong Lian, Yu-Ye Liu, Wei Liu, Yu-Tong Bai, Quan Zhang, Wei Zhang & Jian-Guo Zhang
Abstract Background Deep brain stimulation (DBS) of the nucleus basalis of Meynert (NBM) has shown potential for the treatment of mild-to-moderate Alzheimer’s disease (AD). However, there is little evidence of whether NBM-DBS can improve cognitive functioning in patients with advanced AD. In addition, the mechanisms underlying the modulation of brain networks remain unclear. This study was aimed to assess the cognitive function and the resting-state connectivity following NBM-DBS in patients with advanced AD. Methods Eight...

Simultaneous multi-plane imaging light-sheet fluorescence microscopy for simultaneously acquiring neuronal activity at varying depths

Zhenfei Jiao, Zhou Zhou, Zhongyun Chen, Jinze Xie, Yu Mu, Jiulin Du & Ling Fu
The acquisition of neuronal activities across varying depths with high spatiotemporal resolution is essential for understanding the functional neural circuits in living animals. To address this challenge, we developed SImultaneous MultiPLanE imaging light-sheet fluorescence microscopy (SIMPLE-LSFM) by introducing a digital micromirror device into the illumination arm, which generated multiple sheets in the sample space to simultaneously excite multiple axial depths. Combined with the spherical-aberration-assisted point spread function and the encoded depth information of the detection...

Registration Year

  • 2023
  • 2022
  • 2021

Resource Types

  • Collection


  • Xuan Wu Hospital of the Capital Medical University
  • Fudan University
  • Zhejiang University
  • Capital Medical University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Shanghai Jiao Tong University
  • Huazhong University of Science and Technology
  • Sun Yat-sen University
  • Chinese Academy of Sciences
  • Sichuan University