383 Works

Application of exome sequencing for prenatal diagnosis of fetal structural anomalies: clinical experience and lessons learned from a cohort of 1618 fetuses

Fang Fu, Ru Li, Qiuxia Yu, Dan Wang, Qiong Deng, Lushan Li, Tingying Lei, Guilan Chen, Zhiqiang Nie, Xin Yang, Jin Han, Min Pan, Li Zhen, Yongling Zhang, Xiangyi Jing, Fucheng Li, Fatao Li, Lina Zhang, Cuixing Yi, Yingsi Li, Yan Lu, Hang Zhou, Ken Cheng, Jian Li, Lina Xiang … & Can Liao
Abstract Background Exome sequencing (ES) is becoming more widely available in prenatal diagnosis. However, data on its clinical utility and integration into clinical management remain limited in practice. Herein, we report our experience implementing prenatal ES (pES) in a large cohort of fetuses with anomalies detected by ultrasonography using a hospital-based in-house multidisciplinary team (MDT) facilitated by a three-step genotype-driven followed by phenotype-driven analysis framework. Methods We performed pES in 1618 fetal cases with positive...

Vascular endothelial growth factor and the risk of venous thromboembolism: a genetic correlation and two-sample Mendelian randomization study

Qiaoyun Zhang, Xiaoyu Zhang, Jie Zhang, Biyan Wang, Qiuyue Tian, Xiaoni Meng, Jinxia Zhang, Mengyang Jiang, Yiqiang Zhang, Deqiang Zheng, Lijuan Wu, Wei Wang, Baoguo Wang & Youxin Wang
Abstract Background The relationship between vascular endothelial growth factor (VEGF) and the risk of venous thromboembolism (VTE) has always been one of the concerns in the medical field. However, the causal inferences from published observational studies on this issue may be affected by confounders or reverse causality. We performed a two-sample bidirectional Mendelian randomization (MR) to infer the associations between VEGF and VTE. Methods Summary statistics from genome-wide association studies (GWAS) for VEGF and VTE...

Risk factors and protective factors for nonsuicidal self-injury in adolescents: A hospital- and school-based case–control study

Shuo Geng, Lin Wang, Jinping Sun, Mingdong Xu, Li Zhang, Zhi Yi, Jun Ji & Xu Zhang
ObjectiveNonsuicidal Self-Injury (NSSI) in China has increasingly interested clinicians, although few studies have investigated its risk and protective factors. This study examined the risk factors of NSSI among Chinese adolescents.MethodsThe researchers recruited adolescent participants with NSSI from a hospital outpatient clinic to form a case group and recruited adolescents without NSSI who provided informed consent from the school to form a control group. Participants completed a questionnaire, and data were analyzed using logistic regression.ResultsA total...

Supplementary material from \"Non-canonical role of the sympathetic nervous system in the day-night rhythm in heart rate\"

Cali Anderson, Gabriella Forte, Wei Hu, Henggui Zhang, Mark R. Boyett & Alicia D'Souza
Although for many decades, the day-night rhythm in resting heart rate has been attributed to the parasympathetic branch of the autonomic nervous system (high vagal tone during sleep), recently we have shown that there is a circadian clock in the cardiac pacemaker, the sinus node, and the day-night rhythm in heart rate involves an intrinsic rhythmic transcriptional remodelling of pacemaker ion channels, particularly Hcn4. We have now investigated the role of the sympathetic branch of...

Supplementary material from \"Non-canonical role of the sympathetic nervous system in the day-night rhythm in heart rate\"

Cali Anderson, Gabriella Forte, Wei Hu, Henggui Zhang, Mark R. Boyett & Alicia D'Souza
Although for many decades, the day-night rhythm in resting heart rate has been attributed to the parasympathetic branch of the autonomic nervous system (high vagal tone during sleep), recently we have shown that there is a circadian clock in the cardiac pacemaker, the sinus node, and the day-night rhythm in heart rate involves an intrinsic rhythmic transcriptional remodelling of pacemaker ion channels, particularly Hcn4. We have now investigated the role of the sympathetic branch of...

Structural transformation and performance analysis of PPS-based bag filters in coal-fired power plants

Weilong Cai, Wei Wang, Zhihong Zheng, Jingyun Zhang, Hong Cao, Jianying Huang, Bing Zhang & Yuekun Lai
Owing to the complicated environments, the service life of bag-filter or electrostatic-bag composite precipitators with polyphenylene sulfide (PPS) is greatly deviated from the ideal time. In this paper, the structural transformation of PPS-based bag filter materials collected from the coal-fired power plants with different loading units were investigated systematically. As the SO2 content increases, the surface evolution of PPS fibers from smoothness to crack occurs. An opposite trend is observed for melting point and cross...

Forsythiaside A ameliorates sepsis-induced acute kidney injury via anti-inflammation and antiapoptotic effects by regulating endoplasmic reticulum stress

Yi Chen, Wei Wei, Jingnan Fu, Teng Zhang, Jie Zhao & Tao Ma
Abstract Ethnopharmacological relevance Sepsis is a systemic inflammatory response syndrome caused by an infection in the body, and accompanying acute kidney injury (AKI) is a common complication of sepsis. It is associated with increased mortality and morbidity. Forsythia Fructus, the dried fruit of Forsythia suspensa (Thunb.) Vahl, is a commonly used traditional Chinese medicine. Aims of the study This study aimed to elucidate the protective effect of Forsythiaside A (FTA) on sepsis-induced AKI by downregulating...

A comparison of clinical characteristics of psychiatric inpatients in three hospitals from Western China and America

Min Jia, Bang An, Bin Yan, Qingyan Ma, Binglong Wen, Shanshan Zhao, Chengge Gao, Xiancang Ma, Lili Zhang, Bin Li, Ping Zhang, Jian Wang, Hong Yu & Wei Wang
Abstract Background Different countries have differences in social and cultural context and health system, which may affect the clinical characteristics of psychiatric inpatients. This study was the first to compare cross-cultural differences in the clinical characteristics of psychiatric inpatients in three hospitals from Western China and America. Methods Overall, 905 and 1318 patients from three hospitals, one in America and two in Western China, respectively, were included. We used a standardised protocol and data collection...

Hetrombopag plus porcine ATG and cyclosporine for the treatment of aplastic anaemia: early outcomes of a prospective pilot study

Wenrui Yang, Xin Zhao, Xu Liu, Youzhen Xiong, Huihui Fan, Li Zhang, Jianping Li, Lei Ye, Kang Zhou, Yuan Li, Yang Yang, Guangxin Peng, Liping Jing & Fengkui Zhang
Abstract Hetrombopag, a small molecular thrombopoietin-receptor agonist, has shown encouraging efficiency in immunosuppressive therapy refractory or relapsed severe aplastic anaemia. To investigate the response rate of hetrombopag combined with IST as first-line treatment, we designed a prospective pilot study including 32 patients with SAA treated with anti-human T lymphocyte porcine immunoglobulin (p-ATG), cyclosporine, and hetrombopag. In addition, 96 patients with SAA treated with p-ATG and cyclosporine alone were matched as controls. In total, 21.9% of...

Metabolomics to identify fingerprints of carotid atherosclerosis in nonobese metabolic dysfunction-associated fatty liver disease

Congxiang Shao, Lishu Xu, Pingguang Lei, Wei Wang, Shiting Feng, Junzhao Ye & Bihui Zhong
Abstract Background/aims Nonobese metabolic dysfunction-associated fatty liver disease (MAFLD) is paradoxically associated with improved metabolic and pathological features at diagnosis but similar cardiovascular diseases (CVD) prognosis to obese MAFLD. We aimed to utilize the metabolomics to identify the potential metabolite profiles accounting for this phenomenon. Methods This prospective multicenter cross-sectional study was conducted in China enrolling derivation and validation cohorts. Liquid chromatography coupled with mass spectrometry and gas chromatography-mass spectrometry were applied to perform a...

Identification of critical prognosis signature associated with lymph node metastasis of stomach adenocarcinomas

Xiaohui Wang, Wei Zhang, Yulin Guo, Yifei Zhang, Xiaofeng Bai & Yibin Xie
Abstract Lymph node metastasis (LNM) is an important factor affecting the prognosis of patients with gastric adenocarcinoma (STAD), which is the most common malignancy of the human digestive system. Current detection techniques have limited sensitivity and specificity, and there is a lack of effective biomarkers to screen for LNM. Therefore, it is critical to screen for biomarkers that predict LNM in STAD. Gene expression differential analysis (false discovery rate < 0.05, |log2Fold change| ≥1.5) was...

Metastasis of breast cancer to bones alters the tumor immune microenvironment

Xue Chao, Ying Zhang, Chengyou Zheng, Qitao Huang, Jiabin Lu, Emilia M. Pulver, Julia Houthuijzen, Stefan Hutten, Rongzhen Luo, Jiehua He & Peng Sun
Abstract Background Bone is one of the most frequent sites for breast cancer metastasis. Breast cancer bone metastasis (BCBM) leads to skeletal morbidities including pain, fractures, and spinal compression, all of which severely impact quality of life. Immunotherapy is a promising therapy for patients with advanced cancer, but whether it may provide benefit to metastatic bone cancer is currently unknown. Thus, a better understanding of the immune landscape of bone-disseminated breast cancers may reveal new...

Diagnostic value of combining preoperative inflammatory markers ratios with CA199 for patients with early-stage pancreatic cancer

Yuanlong Gu, Qianjin Hua, Zhipeng Li, Xingli Zhang, Changjie Lou, Yangfen Zhang, Wei Wang, Peiyuan Cai & Juan Zhao
Abstract Background An early diagnosis of pancreatic cancer (PC) is extremely difficult because of the lack of sensitive liquid biopsy methods and effective biomarkers. We attempted to evaluate whether circulating inflammatory marker could complement CA199 for the detection of early-stage PC. Methods We enrolled 430 patients with early-stage PC, 287 patients with other pancreatic tumors (OPT), and 401 healthy controls (HC). The patients and HC were randomly divided into a training set (n = 872)...

Protocatechuic aldehyde acts synergistically with dacarbazine to augment DNA double-strand breaks and promote apoptosis in cutaneous melanoma cells

Junxia Pei, Zhou Su, Xin Zeng, Ya Zhong, Yamei Zhang, Yixi Yang, Qiuxia Lu, Jian Li & Yu Deng
Abstract Background Despite rapid developments in immunotherapy and targeted therapy, dacarbazine (DTIC)-based chemotherapy still has been placed at the first-line for advanced melanoma patients who are after failure of immunotherapy or targeted therapy. However, the limited response rate and survival benefit challenge the DTIC-based chemotherapy for advanced melanoma patients. Methods Two melanoma cell lines, A375 and SK-MEL-28 were cultured with PA and DTIC over a range of concentrations for 72 h and the cell viabilities...

The role of medication adherence in the association between depressive symptoms and quality of life in older adults with type 2 diabetes mellitus

Hao Yang, Fangtuan Wu, Mingdong Gui, Yuwei Cheng & Li Zhang
Abstract Background At present, the role of medication adherence in the association between depressive symptoms and quality of life (QOL) in older adults with type 2 diabetes mellitus (T2DM) was unclear. The purpose of this study was to explore the associations among depressive symptoms, medication adherence and QOL in older adults with T2DM. Methods In this cross-sectional study, 300 older adults with T2DM from the First Affiliated Hospital of Anhui Medical University were enrolled. Among...

Targeting IGF1R signaling enhances the sensitivity of cisplatin by inhibiting proline and arginine metabolism in oesophageal squamous cell carcinoma under hypoxia

Kang Fang, Mingchuang Sun, Zhuyun Leng, Yuan Chu, Ziying Zhao, Zhaoxing Li, Yunwei Zhang, Aiping Xu, Zehua Zhang, Li Zhang, Tao Chen & Meidong Xu
Abstract Background Cisplatin (DDP)-based chemotherapy is commonly adopted as the first-line treatment for patients with oesophageal squamous cell carcinoma (OSCC), but the high rate of drug resistance limits its clinical application and the underlying mechanisms at play remain unclear. The aims of this study were to elucidate the role of abnormal signal transmission and metabolism in the chemoresistance of OSCC under hypoxia and to identify targeted drugs that enhance the sensitivity of DDP chemotherapy. Methods...

FGF19 increases mitochondrial biogenesis and fusion in chondrocytes via the AMPKα-p38/MAPK pathway

Shiyi Kan, Caixia Pi, Li Zhang, Daimo Guo, Zhixing Niu, Yang Liu, Mengmeng Duan, Xiahua Pu, Mingru Bai, Chenchen Zhou, Demao Zhang & Jing Xie
Abstract Fibroblast growth factor 19 (FGF19) is recognized to play an essential role in cartilage development and physiology, and has emerged as a potential therapeutic target for skeletal metabolic diseases. However, FGF19-mediated cellular behavior in chondrocytes remains a big challenge. In the current study, we aimed to investigate the role of FGF19 on chondrocytes by characterizing mitochondrial biogenesis and fission–fusion dynamic equilibrium and exploring the underlying mechanism. We first found that FGF19 enhanced mitochondrial biogenesis...

Self-assembled photonic heterostructure for visible and infrared dual-band anti-counterfeiting

Wenxin Li, Maoren Wang, Jiong Wang, Li Zhang, Linbo Zhang, Longjiang Deng, Jianliang Xie & Peiheng Zhou
Self-assembled photonic structures have greatly expanded the paradigm of optical materials due to their ease of access, the richness of results offered and the strong interaction with light. Among them, photonic heterostructure shows unprecedent advances in exploring novel optical responses, that only can be realized by interfaces or multiple components. In this work, we realize visible and infrared dual-band anti-counterfeiting using metamaterial (MM) - photonic crystal (PhC) heterostructures for the first time. Sedimentation of TiO2...

Explosive eye injuries: characteristics, traumatic mechanisms, and prognostic factors for poor visual outcomes

Ying Zhang, Xin Kang, Qiong Wu, Zhong Zheng, Jun Ying & Mao-Nian Zhang
Abstract Background Explosions can produce blast waves, high-speed medium, thermal radiation, and chemical spatter, leading to complex and compound eye injuries. However, few studies have comprehensively investigated the clinical features of different eye injury types or possible risk factors for poor prognosis. Methods We retrospectively reviewed all consecutive records of explosive eye injuries (1449 eyes in 1115 inpatients) in 14 tertiary referral hospitals in China over 12 years (between January 2008 and December 2019). Data...

Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs

Shunlian Hu, Lu Wang, Yahan Xu, Fajiu Li & Tao Wang
Abstract Background Pulmonary hypertension (PH) is characterized by progressive pulmonary arterial remodelling, associated with different severities of inflammation and altered immune processes. Disulfiram eliminates the formation of N-gasdermin D (GSDMD) plasma membrane pores to prevent pyroptosis. Pyroptosis is a form of lytic cell death characterized by inflammasome activation and proinflammatory cytokine release that acts in the development of PH. We sought to investigate whether disulfiram could alleviate hypoxia-induced PH by inhibiting pyroptosis. Methods To investigate...

Single-cell transcriptome analysis reveals stem cell-like subsets in the progression of Waldenström’s macroglobulinemia

Yu Qiu, Xiao-shuang Wang, Yuan Yao, Yan-min Si, Xue-zhu Wang, Ming-nan Jia, Dao-bin Zhou, Jia Yu, Xin-xin Cao & Jian Li
Abstract Waldenström’s macroglobulinemia (WM) is an uncommon lymphoproliferative disorder, and the precise cellular landscape and the mechanisms of progression from IgM monoclonal gammopathy of undetermined significance (MGUS) to WM remain unclear. We performed single-cell RNA sequencing of CD19 + and CD19-CD38 + cells from healthy donors, IgM MGUS and WM patients. We found that samples from IgM MGUS and WM patients were composed of fewer early B-cell subsets and more T cells and NK cells...

Vascular lipidomics analysis reveales increased levels of phosphocholine and lysophosphocholine in atherosclerotic mice

Li Zhang, Liling Xiong, Li Fan, Haoyang Diao, Mi Tang, Erdan Luo, Wenmei Guo, Xiao Yang & Shasha Xing
Abstract Objective Atherosclerosis (AS) is the major cause of cardiovascular disease, and dyslipidemia is a principal determinant of the initiation and progression of AS. Numerous works have analyzed the lipid signature of blood, but scarce information on the lipidome of vascular tissue is available. This study investigated the lipid profile in the aorta of ApoE−/− mice. Method ApoE−/− mice were randomly divided into two groups: (1) the normal diet (ND) group and (2) the high-fat...

Application of opioid-free general anesthesia for gynecological laparoscopic surgery under ERAS protocol: a non-inferiority randomized controlled trial

Liang Chen, Wensheng He, Xue Liu, Fahui Lv & Yuanhai Li
Abstract Background Enhanced recovery after surgery (ERAS) is now widely used in various surgical fields including gynecological laparoscopic surgery, but the advantages of opioid-free anesthesia (OFA) in gynecological laparoscopic surgery under ERAS protocol are inexact. Aims This study aims to assess the effectiveness and feasibility of OFA technique versus traditional opioid-based anesthesia (OA) technique in gynecological laparoscopic surgery under ERAS. Methods Adult female patients aged 18 ~ 65 years old undergoing gynecological laparoscopic surgery were...

Left atrial reservoir strain measurements derived from intracardiac echocardiography in patients with atrial fibrillation: comparison with transthoracic echocardiography

Jingru Lin, Yuqi Cai, Xu Meng, Shangyu Liu, Fengyang Wang, Limin Liu, Zhenhui Zhu, Mengyi Liu, Ligang Ding, Weichun Wu, Hao Wang & Yan Yao
Abstract Background Intracardiac echocardiography (ICE) provides accurate left atrial (LA) anatomical information in the procedure of atrial fibrillation (AF) ablation but lacks LA functional assessment. LA reservoir strain (LASr) is an excellent marker of LA reservoir function. This study aimed to assess the agreement between LASr derived from ICE and transthoracic echocardiography (TTE) in AF patients and analyze the reproducibility of LASr assessed by ICE combined with speckle tracking imaging. Methods This study prospectively enrolled...

Pinpointing the precise stimulation targets for brain rehabilitation in early-stage Parkinson’s disease

Hanna Lu, Jing Li, Li Zhang, Lin Meng, Yuping Ning & Tianzi Jiang
Abstract Background Transcranial magnetic stimulation (TMS) is increasingly used as a promising non-pharmacological treatment for Parkinson’s disease (PD). Scalp-to-cortex distance (SCD), as a key technical parameter of TMS, plays a critical role in determining the locations of treatment targets and corresponding dosage. Due to the discrepancies in TMS protocols, the optimal targets and head models have yet to be established in PD patients. Objective To investigate the SCDs of the most popular used targets in...

Registration Year

  • 2023
  • 2022
  • 2021

Resource Types

  • Collection


  • Inner Mongolia University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Shanghai Jiao Tong University
  • Huazhong University of Science and Technology
  • Zhejiang University
  • Sun Yat-sen University
  • Shandong University
  • Sichuan University
  • Capital Medical University
  • Fudan University