29 Works

Visinin-like protein 1 levels in blood and CSF as emerging markers for Alzheimer’s and other neurodegenerative diseases

Steffen Halbgebauer, Petra Steinacker, Daniel Riedel, Patrick Oeckl, Sarah Anderl-Straub, Jolina Lombardi, Christine A. F. von Arnim, Magdalena Nagl, Armin Giese, Albert C. Ludolph & Markus Otto
Abstract Background Visinin-like protein 1 (VILIP-1) belongs to the group of emerging biomarkers with the potential to support the early diagnosis of Alzheimer’s disease (AD). However, studies investigating the differential diagnostic potential in cerebrospinal fluid (CSF) are rare and are not available for blood. Methods We set up a novel, sensitive single molecule array (Simoa) assay for the detection of VILIP-1 in CSF and serum. In total, paired CSF and serum samples from 234 patients...

Significant performance enhancement of UV-Vis self-powered CsPbBr3 quantum dot-based photodetectors induced by ligand modification and P3HT embedding

Meng Wang, Dehai Liang, Wen Ma, Qionghua Mo, Zhigang Zang, Qingkai Qian & wensi cai
In this work, we report a novel strategy in improving the performance of UV-Vis self-powered CsPbBr₃ quantum dots (QDs) photodetectors (PDs) by ligand modification and P3HT embedding. Compared with those based on pure QDs, modified PDs show a shorten of response time by nearly 10 times, and an increase of maximum responsivity and specific detectivity for nearly 45 and 97 times, respectively. Such PDs also show a high stability with 90% of the initial photocurrent...

Tissue dissociation for single-cell and single-nuclei RNA sequencing for low amounts of input material

Gordon Wiegleb, Susanne Reinhardt, Andreas Dahl & Nico Posnien
Abstract Background Recent technological advances opened the opportunity to simultaneously study gene expression for thousands of individual cells on a genome-wide scale. The experimental accessibility of such single-cell RNA sequencing (scRNAseq) approaches allowed gaining insights into the cell type composition of heterogeneous tissue samples of animal model systems and emerging models alike. A major prerequisite for a successful application of the method is the dissociation of complex tissues into individual cells, which often requires large...

Supplementary material from \"The road to recovery: a synthesis of outcomes from ecosystem restoration in tropical and sub-tropical Asian forests\"

Lindsay F. Banin, Elizabeth H. Raine, Lucy M. Rowland, Robin L. Chazdon, Stuart W. Smith, Nur Estya Binte Rahman, Adam Butler, Christopher Philipson, Grahame G. Applegate, E. Petter Axelsson, Sugeng Budiharta, Siew Chin Chua, Mark E. J. Cutler, Stephen Elliott, Elva Gemita, Elia Godoong, Laura L. B. Graham, Robin M. Hayward, Andy Hector, Ulrik Ilstedt, Joel Jensen, Srinivasan Kasinathan, Christopher J. Kettle, Daniel Lussetti, Benjapan Manohan … & David F. R. P. Burslem
Current policy is driving renewed impetus to restore forests to return ecological function, protect species, sequester carbon and secure livelihoods. Here we assess the contribution of tree planting to ecosystem restoration in tropical and sub-tropical Asia; we synthesize evidence on mortality and growth of planted trees at 176 sites and assess structural and biodiversity recovery of co-located actively restored and naturally regenerating forest plots. Mean mortality of planted trees was 18% 1 year after planting,...

Regularization approaches in clinical biostatistics: A review of methods and their applications

Sarah Friedrich, Andreas Groll, Katja Ickstadt, Thomas Kneib, Markus Pauly, Jörg Rahnenführer & Tim Friede
A range of regularization approaches have been proposed in the data sciences to overcome overfitting, to exploit sparsity or to improve prediction. Using a broad definition of regularization, namely controlling model complexity by adding information in order to solve ill-posed problems or to prevent overfitting, we review a range of approaches within this framework including penalization, early stopping, ensembling and model averaging. Aspects of their practical implementation are discussed including available R-packages and examples are...

Genome-wide screening for differentially methylated long noncoding RNAs identifies LIFR-AS1 as an epigenetically regulated lncRNA that inhibits the progression of colorectal cancer

Peng Song, Ying Li, Feng Wang, Lingxiao Pu, Linsen Bao, Hengfei Gao, Chuandong Zhu, Meng Wang & Liang Tao
Abstract Background Aberrant DNA methylation is an epigenetic marker that has been linked to the pathogenesis of colorectal cancer (CRC). Long noncoding RNAs (lncRNAs) have been increasingly identified to be associated with tumorigenic processes of CRC. Identifying epigenetically dysregulated lncRNAs and characterizing their effects during carcinogenesis are focuses of cancer research. Methods Differentially methylated loci and expressed lncRNAs were identified by integrating DNA methylome and transcriptome analyses using The Cancer Genome Atlas database. Bisulfite sequencing...

Genome-wide screening for differentially methylated long noncoding RNAs identifies LIFR-AS1 as an epigenetically regulated lncRNA that inhibits the progression of colorectal cancer

Peng Song, Ying Li, Feng Wang, Lingxiao Pu, Linsen Bao, Hengfei Gao, Chuandong Zhu, Meng Wang & Liang Tao
Abstract Background Aberrant DNA methylation is an epigenetic marker that has been linked to the pathogenesis of colorectal cancer (CRC). Long noncoding RNAs (lncRNAs) have been increasingly identified to be associated with tumorigenic processes of CRC. Identifying epigenetically dysregulated lncRNAs and characterizing their effects during carcinogenesis are focuses of cancer research. Methods Differentially methylated loci and expressed lncRNAs were identified by integrating DNA methylome and transcriptome analyses using The Cancer Genome Atlas database. Bisulfite sequencing...

Monitoring and management of chronic kidney disease in ambulatory care – analysis of clinical and claims data from a population-based study

Gesine Weckmann, Janine Wirkner, Elisa Kasbohm, Carolin Zimak, Annekathrin Haase, Jean-François Chenot, Carsten Oliver Schmidt & Sylvia Stracke
Abstract Background Although chronic kidney disease (CKD) is highly prevalent in the general population, little research has been conducted on CKD management in ambulatory care. Objective was to assess management and quality of care by evaluating CKD coding in ambulatory care, patient diagnosis awareness, frequency of monitoring and whether appropriate patients are referred to nephrology. Methods Clinical data from the population-based cohort Study of Health in Pomerania (SHIP-START) were matched with claims data of the...

Attention to visual motion suppresses neuronal and behavioral sensitivity in nearby feature space

Sang-Ah Yoo, Julio C. Martinez-Trujillo, Stefan Treue, John K. Tsotsos & Mazyar Fallah
Abstract Background Feature-based attention prioritizes the processing of the attended feature while strongly suppressing the processing of nearby ones. This creates a non-linearity or “attentional suppressive surround” predicted by the Selective Tuning model of visual attention. However, previously reported effects of feature-based attention on neuronal responses are linear, e.g., feature-similarity gain. Here, we investigated this apparent contradiction by neurophysiological and psychophysical approaches. Results Responses of motion direction-selective neurons in area MT/MST of monkeys were recorded...

Regularization approaches in clinical biostatistics: A review of methods and their applications

Sarah Friedrich, Andreas Groll, Katja Ickstadt, Thomas Kneib, Markus Pauly, Jörg Rahnenführer & Tim Friede
A range of regularization approaches have been proposed in the data sciences to overcome overfitting, to exploit sparsity or to improve prediction. Using a broad definition of regularization, namely controlling model complexity by adding information in order to solve ill-posed problems or to prevent overfitting, we review a range of approaches within this framework including penalization, early stopping, ensembling and model averaging. Aspects of their practical implementation are discussed including available R-packages and examples are...

Differential in vitro effects of targeted therapeutics in primary human liver cancer: importance for combined liver cancer

Ihtzaz Ahmed Malik, Mansi Rajput, Rieke Werner, Dorothea Fey, Niloofar Salehzadeh, Christine A. F. von Arnim & Jörg Wilting
Abstract The incidence of primary liver tumors, hepatocellular carcinoma (HCC), intrahepatic cholangiocellular carcinoma (ICC), and combined HCC/ICC (cHCC/CC) is increasing. For ICC, targeted therapy exists only for a small subpopulation of patients, while for HCC, Sorafenib and Lenvatinib are in use. Diagnosis of cHCC/CC is a great challenge and its incidence is underestimated, bearing the risk of unintended non-treatment of ICC. Here, we investigated effects of targeted inhibitors on human ICC cell lines (HUH28, RBE,...

Validation of the Self-Rating of Biological Rhythm Disorder for Adolescents (SBRDA) Scale by Dim Light Melatonin Onset in Healthy Young Adults

Yang Xie, Xiaoyan Wu, Xingyue Mou, Meng Wang, Shuman Tao, Yuhui Wan & Fangbiao Tao
Understanding the biological rhythms that influence young adult health is vital because the combination of biological changes and a circadian phase delay lead to young adults being at high risk of circadian misalignment. We have previously established a self-rating of biological rhythm disorder for adolescents (SBRDA). However, we did not externally validate the SBRDA against objective measures of biological rhythms such as dim light melatonin onset (DLMO)—the gold standard of the endogenous circadian phase. The...

Comparison of Quality of Life Between Breast Cancer Patients Treated With and Without Adjunctive Traditional Chinese Medicine in Taiwan

Yeong-Ruey Chu, Pei-Tseng Kung, Liang-Chih Liu, Chin-Yao Lin, Fu Ou-Yang, Chia-Herng Yue, Shan-Yu Su, Ying-Yu Chen, Wen-Ching Wang, Hui-Fen Kao, Wen-Yu Chou & Wen-Chen Tsai
In Taiwan, breast cancer has the highest incidence among all cancers. Although adjunctive traditional Chinese medicine treatment (TCM) have been used to ameliorate the side effects or discomfort caused by cancer treatments, no study has focused on the assessment of the quality of life of patients undergoing adjunctive TCM treatments. This study compared the quality of life between breast cancer patients treated with and without adjunctive TCM. Questionnaires were collected from 7 hospitals with a...

Accuracy of intraoral real-time navigation versus static, CAD/CAM-manufactured pilot drilling guides in dental implant surgery: an in vitro study

Robert Stünkel, Alexander-Nicolai Zeller, Thomas Bohne, Florian Böhrnsen, Edris Wedi, David Raschke & Philipp Kauffmann
Abstract Background Nowadays, 3D planning and static for dynamic aids play an increasing role in oral rehabilitation of the masticatory apparatus with dental implants. The aim of this study is to compare the accuracy of implant placement using a 3D-printed drilling guide and an intraoral real-time dynamic navigation system. Methods A total of 60 implants were placed on 12 partially edentulous lower jaw models. 30 were placed with pilot drilling guides, the other half with...

Monitoring and management of chronic kidney disease in ambulatory care – analysis of clinical and claims data from a population-based study

Gesine Weckmann, Janine Wirkner, Elisa Kasbohm, Carolin Zimak, Annekathrin Haase, Jean-François Chenot, Carsten Oliver Schmidt & Sylvia Stracke
Abstract Background Although chronic kidney disease (CKD) is highly prevalent in the general population, little research has been conducted on CKD management in ambulatory care. Objective was to assess management and quality of care by evaluating CKD coding in ambulatory care, patient diagnosis awareness, frequency of monitoring and whether appropriate patients are referred to nephrology. Methods Clinical data from the population-based cohort Study of Health in Pomerania (SHIP-START) were matched with claims data of the...

Comparison of Quality of Life Between Breast Cancer Patients Treated With and Without Adjunctive Traditional Chinese Medicine in Taiwan

Yeong-Ruey Chu, Pei-Tseng Kung, Liang-Chih Liu, Chin-Yao Lin, Fu Ou-Yang, Chia-Herng Yue, Shan-Yu Su, Ying-Yu Chen, Wen-Ching Wang, Hui-Fen Kao, Wen-Yu Chou & Wen-Chen Tsai
In Taiwan, breast cancer has the highest incidence among all cancers. Although adjunctive traditional Chinese medicine treatment (TCM) have been used to ameliorate the side effects or discomfort caused by cancer treatments, no study has focused on the assessment of the quality of life of patients undergoing adjunctive TCM treatments. This study compared the quality of life between breast cancer patients treated with and without adjunctive TCM. Questionnaires were collected from 7 hospitals with a...

Dementia risk prediction in individuals with mild cognitive impairment: a comparison of Cox regression and machine learning models

Meng Wang, Matthew Greenberg, Nils D. Forkert, Thierry Chekouo, Gabriel Afriyie, Zahinoor Ismail, Eric E. Smith & Tolulope T. Sajobi
Abstract Background Cox proportional hazards regression models and machine learning models are widely used for predicting the risk of dementia. Existing comparisons of these models have mostly been based on empirical datasets and have yielded mixed results. This study examines the accuracy of various machine learning and of the Cox regression models for predicting time-to-event outcomes using Monte Carlo simulation in people with mild cognitive impairment (MCI). Methods The predictive accuracy of nine time-to-event regression...

Visinin-like protein 1 levels in blood and CSF as emerging markers for Alzheimer’s and other neurodegenerative diseases

Steffen Halbgebauer, Petra Steinacker, Daniel Riedel, Patrick Oeckl, Sarah Anderl-Straub, Jolina Lombardi, Christine A. F. von Arnim, Magdalena Nagl, Armin Giese, Albert C. Ludolph & Markus Otto
Abstract Background Visinin-like protein 1 (VILIP-1) belongs to the group of emerging biomarkers with the potential to support the early diagnosis of Alzheimer’s disease (AD). However, studies investigating the differential diagnostic potential in cerebrospinal fluid (CSF) are rare and are not available for blood. Methods We set up a novel, sensitive single molecule array (Simoa) assay for the detection of VILIP-1 in CSF and serum. In total, paired CSF and serum samples from 234 patients...

Differential in vitro effects of targeted therapeutics in primary human liver cancer: importance for combined liver cancer

Ihtzaz Ahmed Malik, Mansi Rajput, Rieke Werner, Dorothea Fey, Niloofar Salehzadeh, Christine A. F. von Arnim & Jörg Wilting
Abstract The incidence of primary liver tumors, hepatocellular carcinoma (HCC), intrahepatic cholangiocellular carcinoma (ICC), and combined HCC/ICC (cHCC/CC) is increasing. For ICC, targeted therapy exists only for a small subpopulation of patients, while for HCC, Sorafenib and Lenvatinib are in use. Diagnosis of cHCC/CC is a great challenge and its incidence is underestimated, bearing the risk of unintended non-treatment of ICC. Here, we investigated effects of targeted inhibitors on human ICC cell lines (HUH28, RBE,...

Significant performance enhancement of UV-Vis self-powered CsPbBr3 quantum dot-based photodetectors induced by ligand modification and P3HT embedding

Meng Wang, Dehai Liang, Wen Ma, Qionghua Mo, Zhigang Zang, Qingkai Qian & wensi cai
In this work, we report a novel strategy in improving the performance of UV-Vis self-powered CsPbBr₃ quantum dots (QDs) photodetectors (PDs) by ligand modification and P3HT embedding. Compared with those based on pure QDs, modified PDs show a shorten of response time by nearly 10 times, and an increase of maximum responsivity and specific detectivity for nearly 45 and 97 times, respectively. Such PDs also show a high stability with 90% of the initial photocurrent...

Significant performance enhancement of UV-Vis self-powered CsPbBr3 quantum dot-based photodetectors induced by ligand modification and P3HT embedding

Meng Wang, Dehai Liang, Wen Ma, Qionghua Mo, Zhigang Zang, Qingkai Qian & wensi cai
In this work, we report a novel strategy in improving the performance of UV-Vis self-powered CsPbBr₃ quantum dots (QDs) photodetectors (PDs) by ligand modification and P3HT embedding. Compared with those based on pure QDs, modified PDs show a shorten of response time by nearly 10 times, and an increase of maximum responsivity and specific detectivity for nearly 45 and 97 times, respectively. Such PDs also show a high stability with 90% of the initial photocurrent...

Accuracy of intraoral real-time navigation versus static, CAD/CAM-manufactured pilot drilling guides in dental implant surgery: an in vitro study

Robert Stünkel, Alexander-Nicolai Zeller, Thomas Bohne, Florian Böhrnsen, Edris Wedi, David Raschke & Philipp Kauffmann
Abstract Background Nowadays, 3D planning and static for dynamic aids play an increasing role in oral rehabilitation of the masticatory apparatus with dental implants. The aim of this study is to compare the accuracy of implant placement using a 3D-printed drilling guide and an intraoral real-time dynamic navigation system. Methods A total of 60 implants were placed on 12 partially edentulous lower jaw models. 30 were placed with pilot drilling guides, the other half with...

Attention to visual motion suppresses neuronal and behavioral sensitivity in nearby feature space

Sang-Ah Yoo, Julio C. Martinez-Trujillo, Stefan Treue, John K. Tsotsos & Mazyar Fallah
Abstract Background Feature-based attention prioritizes the processing of the attended feature while strongly suppressing the processing of nearby ones. This creates a non-linearity or “attentional suppressive surround” predicted by the Selective Tuning model of visual attention. However, previously reported effects of feature-based attention on neuronal responses are linear, e.g., feature-similarity gain. Here, we investigated this apparent contradiction by neurophysiological and psychophysical approaches. Results Responses of motion direction-selective neurons in area MT/MST of monkeys were recorded...

Supplementary material from \"The road to recovery: a synthesis of outcomes from ecosystem restoration in tropical and sub-tropical Asian forests\"

Lindsay F. Banin, Elizabeth H. Raine, Lucy M. Rowland, Robin L. Chazdon, Stuart W. Smith, Nur Estya Binte Rahman, Adam Butler, Christopher Philipson, Grahame G. Applegate, E. Petter Axelsson, Sugeng Budiharta, Siew Chin Chua, Mark E. J. Cutler, Stephen Elliott, Elva Gemita, Elia Godoong, Laura L. B. Graham, Robin M. Hayward, Andy Hector, Ulrik Ilstedt, Joel Jensen, Srinivasan Kasinathan, Christopher J. Kettle, Daniel Lussetti, Benjapan Manohan … & David F. R. P. Burslem
Current policy is driving renewed impetus to restore forests to return ecological function, protect species, sequester carbon and secure livelihoods. Here we assess the contribution of tree planting to ecosystem restoration in tropical and sub-tropical Asia; we synthesize evidence on mortality and growth of planted trees at 176 sites and assess structural and biodiversity recovery of co-located actively restored and naturally regenerating forest plots. Mean mortality of planted trees was 18% 1 year after planting,...

Tissue dissociation for single-cell and single-nuclei RNA sequencing for low amounts of input material

Gordon Wiegleb, Susanne Reinhardt, Andreas Dahl & Nico Posnien
Abstract Background Recent technological advances opened the opportunity to simultaneously study gene expression for thousands of individual cells on a genome-wide scale. The experimental accessibility of such single-cell RNA sequencing (scRNAseq) approaches allowed gaining insights into the cell type composition of heterogeneous tissue samples of animal model systems and emerging models alike. A major prerequisite for a successful application of the method is the dissociation of complex tissues into individual cells, which often requires large...

Registration Year

  • 2023
    4
  • 2022
    25

Resource Types

  • Collection
    29

Affiliations

  • University of Göttingen
    29
  • Zhejiang University
    11
  • Southern Medical University
    11
  • Shanghai Jiao Tong University
    11
  • Sixth Affiliated Hospital of Sun Yat-sen University
    9
  • Beijing University of Chemical Technology
    9
  • Lanzhou Veterinary Research Institute
    9
  • Stanford University
    9
  • Xuan Wu Hospital of the Capital Medical University
    9
  • Huazhong University of Science and Technology
    9