1,883 Works

LPAR5 confers radioresistance to cancer cells associated with EMT activation via the ERK/Snail pathway

Xiao-Ya Sun, Hao-Zheng Li, Da-Fei Xie, Shan-Shan Gao, Xin Huang, Hua Guan, Chen-Jun Bai & Ping-Kun Zhou
Abstract Background Epithelial-to-mesenchymal transition (EMT) is a critical event contributing to more aggressive phenotypes in cancer cells. EMT is frequently activated in radiation-targeted cells during the course of radiotherapy, which often endows cancers with acquired radioresistance. However, the upstream molecules driving the signaling pathways of radiation-induced EMT have not been fully delineated. Methods In this study, RNA-seq-based transcriptome analysis was performed to identify the early responsive genes of HeLa cells to γ-ray irradiation. EMT-associated genes...

Breaking the bandwidth limit of a high-quality-factor ring modulator based on thin-film lithium niobate

Yu Xue, RanFeng Gan, kaixuan chen, gengxin chen, ziliang ruan, Junwei Zhang, Jie Liu, Daoxin Dai, Changjian Guo & Liu Liu
Growing global data traffic requires high-performance modulators with a compact size, a large bandwidth, a low optical loss, and a small power consumption. A careful trade-off among these parameters usually has to be made when designing such a device. Here, we propose and demonstrate an electro-optic ring modulator on the thin-film lithium niobate platform without compromising between any performances. The device exhibits a low on-chip loss of about -0.15dB with a high intrinsic Q-factor of...

Histological and molecular responses of Vigna angularis to Uromyces vignae infection

Xiwang Ke, Jie Wang, Xiaodan Xu, Yongxia Guo, Yuhu Zuo & Lihua Yin
Abstract Background To advance the understanding of adzuki bean (Vigna angularis) resistance to infection with the rust-causing fungus Uromyces vignae (Uv), we comprehensively analyzed histological events and the transcriptome of Uv-infected adzuki bean. Results Compared with the susceptible cv. Baoqinghong (BQH), the resistant cv. QH1 showed inhibition of uredospore germination and substomatal vesicle development, intense autofluorescence of cells around the infection site, and cell wall deposit formation in response to Uv infection. In cv. QH1,...

Prevalence of reproductive tract infections among women preparing to conceive in Chongqing, China: trends and risk factors

Jun Liu, Mengyao Zeng, Liu Yang, Yanyan Mao, Yang He, Min Li, Qing Chen, Weijin Zhou, Liang Chen & Qianxi Zhu
Abstract Background Reproductive tract infection has become a major public health issue all over the world for its high and growing prevalence. It can cause adverse pregnancy outcomes in pregnant women and their foetuses. This study aimed to investigate the trends and risk factors of the prevalence of reproductive tract infections among women who prepared to conceive in the Chongqing Municipality (China) from 2012 to 2016. Methods A multi-center cross-sectional study was conducted between January...

Identification of DNA methylation-regulated genes as potential biomarkers for coronary heart disease via machine learning in the Framingham Heart Study

Xiaokang Zhang, Chen Wang, Dingdong He, Yating Cheng, Li Yu, Daoxi Qi, Boyu Li & Fang Zheng
Abstract Background DNA methylation-regulated genes have been demonstrated as the crucial participants in the occurrence of coronary heart disease (CHD). The machine learning based on DNA methylation-regulated genes has tremendous potential for mining non-invasive predictive biomarkers and exploring underlying new mechanisms of CHD. Results First, the 2085 age-gender-matched individuals in Framingham Heart Study (FHS) were randomly divided into training set and validation set. We then integrated methylome and transcriptome data of peripheral blood leukocytes (PBLs)...

Mucosal-associated invariant T cells predict increased acute graft-versus-host-disease incidence in patients receiving allogeneic hematopoietic stem cell transplantation

Zhao Wang, Sudong Zhang, Xiaoyu Zhang, Li Liu, Lukun Zhou, Yuyan Shen, Rongli Zhang, Yi He, Donglin Yang, Erlie Jiang, Xiaoming Feng, Jiaxi Zhou, Tao Cheng, Mingzhe Han & Sizhou Feng
Abstract Background Mucosal-associated invariant T (MAIT) cells are innate-like T cells, some studies have reported that the number of circulating MAIT cells reduced in patients with acute graft-versus-host-disease (aGVHD) development. However, the role of donor MAIT cells on aGVHD development and subsequent functional change still remain unclear. Methods The study recruited 86 patients with hematological malignancies who underwent allogeneic hematopoietic cell transplantation (HCT) from May 1, 2018 to June 30, 2019. MAIT cells, their subset,...

Efficacy of neoadjuvant docetaxel + cisplatin chemo-hormonal therapy versus docetaxel chemo-hormonal therapy in patients with locally advanced prostate cancer with germline DNA damage repair gene alterations

Chenfei Chi, Jiazhou Liu, Liancheng Fan, Yinjie Zhu, Yanqing Wang, Jianjun Sha, Yiran Huang, Baijun Dong, Jiahua Pan & Wei Xue
Purpose:To assess the efficacy and safety of neoadjuvant docetaxel + cisplatin chemotherapy with androgen deprivation therapy for the treatment of locally advanced prostate cancer (PCa) in patients harboring germline DNA damage repair genes (gDDR) defects.Methods:We conducted a prospective observational study in patients with locally advanced PCa confirmed with gDDR defects through next-generation sequencing. All patients received either docetaxel + cisplatin (platinum-group) or docetaxel chemo-hormonal therapy (docetaxel group) followed by radical prostatectomy with extended lymphadenectomy. The...

Associations between inflammatory bowel disease, social isolation, and mortality: evidence from a longitudinal cohort study

Jie Chen, Jiawei Geng, Jiayi Wang, Zhenhua Wu, Tian Fu, Yuhao Sun, Xuejie Chen, Xiaoyan Wang & Therese Hesketh
Background:Social well-being of patients with inflammatory bowel disease (IBD) is garnering increased attention; however, the impact of social isolation remained poorly understood.Objectives:We investigated the joint association of social isolation and IBD with premature deaths to articulate the profound impact of social isolation in IBD prognosis.Design:Longitudinal cohort study.Methods:We leveraged data of 486,014 participants from UK Biobank (including 5791 with IBD), the mean follow-up was 11.84 years. Diagnoses of IBD and its subtypes of Crohn’s disease (CD)...

High-energy Q switched Yb-doped fiber laser based on a ternary layered structured Ti2AlC saturable absorber

KANG ZHANG, Ming Feng, Jinyue Xie, Xu Sang, Guoqing Sun, Ye Tian, Feng Song & Wei Huang
Ti₂AlC is a kind of ternary layered structured ceramic metal compound, combing advantages of both ceramic and metal. Herein, the saturable absorption performance of Ti₂AlC at 1 μm waveband is investigated for the first time. The Ti₂AlC behaves with excellent saturable absorption, which has a modulation depth of 14.53% and a saturable intensity of 13.27 MW/cm². An all-normal dispersion fiber laser based on the Ti₂AlC saturable absorber (SA) is constructed. The repetition frequency of the...

Cardiac-targeted PIASy gene silencing mediates deSUMOylation of caveolin-3 and prevents ischemia/reperfusion-induced Nav1.5 downregulation and ventricular arrhythmias

Chen-Chen Hu, Xin Wei, Jin-Min Liu, Lin-Lin Han, Cheng-Kun Xia, Jing Wu, Tao You, A.-Fang Zhu, Shang-Long Yao, Shi-Ying Yuan, Hao-Dong Xu, Zheng-Yuan Xia, Ting-Ting Wang & Wei-Ke Mao
Abstract Background Abnormal myocardial Nav1.5 expression and function cause lethal ventricular arrhythmias during myocardial ischemia–reperfusion (I/R). Protein inhibitor of activated STAT Y (PIASy)-mediated caveolin-3 (Cav-3) SUMO modification affects Cav-3 binding to the voltage-gated sodium channel 1.5 (Nav1.5). PIASy activity is increased after myocardial I/R, but it is unclear whether this is attributable to plasma membrane Nav1.5 downregulation and ventricular arrhythmias. Methods Using recombinant adeno-associated virus subtype 9 (AAV9), rat cardiac PIASy was silenced using intraventricular...

The role of proteoglycan form of DMP1 in cranial repair

Yang Liu, Pingping Niu, Mengqi Zhou & Hui Xue
Abstract Background The cranial region is a complex set of blood vessels, cartilage, nerves and soft tissues. The reconstruction of cranial defects caused by trauma, congenital defects and surgical procedures presents clinical challenges. Our previous data showed that deficiency of the proteoglycan (PG) form of dentin matrix protein 1 (DMP1-PG) could lead to abnormal cranial development. In addition, DMP1-PG was highly expressed in the cranial defect areas. The present study aimed to investigate the potential...

Human umbilical cord mesenchymal stem cells combined with pirfenidone upregulates the expression of RGS2 in the pulmonary fibrosis in mice

Xian Wu, Hao Gou, Ou Zhou, Huijun Qiu, Hanmin Liu, Zhou Fu & Lina Chen
Abstract Objective The therapeutic effect of umbilical cord-derived mesenchymal stem cells (hUC-MSCs) in combination with pirfenidone (PFD) on pulmonary fibrosis in mice and its possible mechanism were investigated. Methods C57BL/6 mice were randomly divided into six groups: control group, model group, P10 group, P30 group, P100 group, and P300 group. Modeled by tracheal intubation with 3 mg/kg bleomycin drip, each dose of PFD was administered daily by gavage from day 7 onwards. The mice were...

Fibroblast growth factor 10 attenuates chronic obstructive pulmonary disease by protecting against glycocalyx impairment and endothelial apoptosis

Tian Jiang, Weiping Hu, Shaoyuan Zhang, Changhao Ren, Siyun Lin, Zhenyu Zhou, Hao Wu, Jun Yin & Lijie Tan
Abstract Background The defects and imbalance in lung repair and structural maintenance contribute to the pathogenesis of chronic obstructive pulmonary diseases (COPD), yet the molecular mechanisms that regulate lung repair process are so far incompletely understood. We hypothesized that cigarette smoking causes glycocalyx impairment and endothelial apoptosis in COPD, which could be repaired by the stimulation of fibroblast growth factor 10 (FGF10)/FGF receptor 1 (FGFR1) signaling. Methods We used immunostaining (immunohistochemical [IHC] and immunofluorescence [IF])...

The FUS/circEZH2/KLF5/ feedback loop contributes to CXCR4-induced liver metastasis of breast cancer by enhancing epithelial-mesenchymal transition

Peng Liu, Zehao Wang, Xueqi Ou, Peng Wu, Yue Zhang, Song Wu, Xiangsheng Xiao, Yuehua Li, Feng Ye & Hailin Tang
Abstract Background Metastasis of breast cancer have caused the majority of cancer-related death worldwide. The circRNAs are associated with tumorigenesis and metastasis in breast cancer according to recent research. However, the biological mechanism of circRNAs in liver metastatic breast cancer remains ambiguous yet. Methods Microarray analysis of three pairs of primary BC tissues and matched hepatic metastatic specimens identified circEZH2. We used RT-qPCR and FISH assays to confirm circEZH2 existence, characteristics, and expression. Both in...

Exosome-like nanovesicles derived from Phellinus linteus inhibit Mical2 expression through cross-kingdom regulation and inhibit ultraviolet-induced skin aging

Jingxia Han, Ting Wu, Jing Jin, Zhiyang Li, Wenjun Cheng, Xintong Dai, Kai Yang, Heng Zhang, Zhiyuan Zhang, Haohao Zhang, Rong Fan, Shaoting Zheng, Haoyang Liu, Yinan Li, Huan Zhao, Cheng Yao, Tingting Lin, Caibin Zhu & Huijuan Liu
Abstract Background Phellinus linteus (PL), which is a typical medicinal fungus, has been shown to have antitumor and anti-inflammatory activities. However, studies on the effect of anti-photoaging are limited. Studies have shown that exosome-like nanovesicles are functional components of many medicinal plants, and miRNAs in exosome-like nanovesicles play a cross-kingdom regulatory role. At present, research on fungi exosome-like nanovesicles (FELNVs) is few. Results We systematically evaluated the anti-aging effects of PL. FELNVs of PL were...

Electroacupuncture alleviates cognitive dysfunction and neuronal pyroptosis in septic mice

Yan Li, Zhaoying Li, Fujuan He, Chenguang Qin, Rui Fan, Fangxiang Zhang & Bin Wang
Background:Sepsis is defined as organ dysfunction caused by an uncontrolled response to infection and is followed by a high incidence of cognitive dysfunction, which can severely affect patients’ quality of life. Previous studies have suggested that electroacupuncture (EA) is protective against sepsis-associated cognitive dysfunction and that pyroptosis plays a vital role in cognitive function. The aim of this study was to investigate the effect of EA on cognition and neuronal pyroptosis in a mouse model...

An area under the concentration–time curve threshold as a predictor of efficacy and nephrotoxicity for individualizing polymyxin B dosing in patients with carbapenem-resistant gram-negative bacteria

Jing Yang, Shaohua Liu, Jingli Lu, Tongwen Sun, Peile Wang & Xiaojian Zhang
Abstract Background Evidence supports therapeutic drug monitoring of polymyxin B, but clinical data for establishing an area under the concentration–time curve across 24 h at steady state (AUCss,24 h) threshold are still limited. This study aimed to examine exposure–response/toxicity relationship for polymyxin B to establish an AUCss,24 h threshold in a real-world cohort of patients. Methods Using a validated Bayesian approach to estimate AUCss,24 h from two samples, AUCss,24 h threshold that impacted the risk...

Zinc oxide nanoparticle regulates the ferroptosis, proliferation, invasion and steaminess of cervical cancer by miR-506-3p/CD164 signaling

Jie-Yun Lei, Shuang-Xue Li, Feng Li, Hui Li & Yuan-Sheng Lei
Abstract Background Cancer stem cell (CSC) and ferroptosis play critical roles in cancer development, but the underlying mechanisms remain unclear. Cervical cancer induces a great mortality and an increased incidence globally. Zinc oxide nanoparticle is the nanomaterial that has been applied in industrial products and targets multiple cancer cell types and cancer stem cells. Here, we aimed to explore the effect of ZON on CSC and ferroptosis of cervical cancer. Methods In the present study,...

Zinc oxide nanoparticle regulates the ferroptosis, proliferation, invasion and steaminess of cervical cancer by miR-506-3p/CD164 signaling

Jie-Yun Lei, Shuang-Xue Li, Feng Li, Hui Li & Yuan-Sheng Lei
Abstract Background Cancer stem cell (CSC) and ferroptosis play critical roles in cancer development, but the underlying mechanisms remain unclear. Cervical cancer induces a great mortality and an increased incidence globally. Zinc oxide nanoparticle is the nanomaterial that has been applied in industrial products and targets multiple cancer cell types and cancer stem cells. Here, we aimed to explore the effect of ZON on CSC and ferroptosis of cervical cancer. Methods In the present study,...

The presence of ovarian endometrioma adversely affect ovarian reserve and response to stimulation but not oocyte quality or IVF/ICSI outcomes: a retrospective cohort study

Cheng Zeng, Ruihui Lu, Xin Li, Yanrong Kuai, Sheng Wang & Qing Xue
Abstract Background The possible impact of ovarian endometriomas (OMAs) on in vitro fertilization (IVF) outcomes remains controversial. Therefore, this study aimed to assess the impact of OMAs on IVF cycle parameters, including ovarian reserve and response to stimulation, embryo quality and pregnancy outcomes. Methods This retrospective cohort study included 2067 patients undergoing their first IVF/ICSI cycles between January 2018 and December 2020. The study group included 154 infertile women who had OMAs. The control group...

Penalized Spline-Involved Tree-based (PenSIT) Learning for estimating an optimal dynamic treatment regime using observational data

Kelly A Speth, Michael R Elliott, Juan L Marquez & Lu Wang
Dynamic treatment regimes are a set of time-adaptive decision rules that can be used to personalize treatment across multiple stages of care. Grounded in causal inference methods, dynamic treatment regimes identify variables that differentiate the treatment effect and may be used to tailor treatments across individuals based on the patient’s own characteristics – thereby representing an important step toward personalized medicine. In this manuscript we introduce Penalized Spline-Involved Tree-based Learning, which seeks to improve upon...

Emergency department-based, nurse-initiated, serious illness conversation intervention for older adults: a protocol for a randomized controlled trial

Thidathit Prachanukool, Susan D. Block, Donna Berry, Rachel S. Lee, Sarah Rossmassler, Mohammad A. Hasdianda, Wei Wang, Rebecca Sudore, Mara A. Schonberg, James A. Tulsky & Kei Ouchi
Abstract Background Visits to the emergency department (ED) are inflection points in patients’ illness trajectories and are an underutilized setting to engage seriously ill patients in conversations about their goals of care. We developed an intervention (ED GOAL) that primes seriously ill patients to discuss their goals of care with their outpatient clinicians after leaving the ED. The aims of this study are (i) to test the impact of ED GOAL administered by trained nurses...

The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes

Yang Yang, Yiqi Wu, Mengting Ji, Xiaoyin Rong, Yanwei Zhang, Shuai Yang, Chang Lu, Chunbo Cai, Pengfei Gao, Xiaohong Guo, Bugao Li & Guoqing Cao
Abstract Long non-coding RNAs (lncRNAs) play a vital role in regulating adipogenesis. However, the associated regulatory mechanisms have yet to be described in detail in pig. In this study, we demonstrate a critical role for lncMYOZ2 in adipogenesis from porcine preadipocytes. Specifically, lncMYOZ2 was more abundant in the adipose tissue of Mashen (fat-type) pigs than for Large White (lean-type) pigs, and knockdown of this lncRNA significantly inhibited the differentiation of porcine preadipocytes into adipocytes. Mechanistically,...

3D-printed hemipelvic prosthesis combined with a dual mobility bearing in patients with primary malignant neoplasm involving the acetabulum: clinical outcomes and finite element analysis

Miao Wang, Tianze Liu, Changli Xu, Chang Liu, Bo Li, Qiujian Lian, Tongjiang Chen, Suchi Qiao & Zhiwei Wang
Abstract Background Limb salvage reconstruction for pelvic tumors, especially periacetabular tumors, is challenging. We combined the use of dual mobility bearing and 3D-printed hemipelvic prosthesis to improve function and reduce the probability of complications after hemi-pelvic resection in patients with primary acetabular malignancy. The purpose of this study was to evaluate the efficacy and safety of this combination. Methods Between October 2011 and May 2021, 11 patients with malignancies involving the acetabulum received hemipelvic replacement...

Using a mixed method to identify communication skills training priorities for Chinese general practitioners in diabetes care

Mi Yao, Gang Yuan, Kai Lin, Lijuan Liu, Hao Tang, Jieying Xie, Xinxin Ji, Rongxin Wang, Binkai Li, Jiajia Hao, Huichang Qiu, Dongying Zhang, Hai Li, Shamil Haroon, Dawn Jackson, Wei Chen, Kar Keung Cheng & Richard Lehman
Abstract Background In China diabetes care is gradually shifting from secondary to primary care with great infrastructure investment and GP training. However, most GPs in China lack communication skills training, which is a huge obstacle in communication with their patients in primary care. In this study we seek to identify training priorities that is evidence-based, appropriate for the context of primary care in China, and that meet the real needs of both GPs and people...

Registration Year

  • 2022

Resource Types

  • Collection


  • Shanghai Jiao Tong University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Zhejiang University
  • Fudan University
  • Sichuan University
  • Capital Medical University
  • Huazhong University of Science and Technology
  • Sun Yat-sen University
  • Chinese Academy of Sciences
  • Nanjing Medical University