4 Works

Deeper genomic insights into tomato CLE genes repertoire identify new active peptides

Samy Carbonnel, Laurent Falquet & Ora Hazak
Abstract Background In eukaryotes, cell-to-cell communication relies on the activity of small signaling peptides. In plant genomes, many hundreds of genes encode for such short peptide signals. However, only few of them are functionally characterized and due to the small gene size and high sequence variability, the comprehensive identification of such peptide-encoded genes is challenging. The CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION-RELATED (CLE) gene family encodes for short peptides that have a role in plant meristem maintenance,...

Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

Aura Maria Moreno-Echeverri, Eva Susnik, Dimitri Vanhecke, Patricia Taladriz-Blanco, Sandor Balog, Alke Petri-Fink & Barbara Rothen-Rutishauser
Abstract Background In the field of nanoscience there is an increasing interest to follow dynamics of nanoparticles (NP) in cells with an emphasis on endo-lysosomal pathways and long-term NP fate. During our research on this topic, we encountered several pitfalls, which can bias the experimental outcome. We address some of these pitfalls and suggest possible solutions. The accuracy of fluorescence microscopy methods has an important role in obtaining insights into NP interactions with lysosomes at...

Deeper genomic insights into tomato CLE genes repertoire identify new active peptides

Samy Carbonnel, Laurent Falquet & Ora Hazak
Abstract Background In eukaryotes, cell-to-cell communication relies on the activity of small signaling peptides. In plant genomes, many hundreds of genes encode for such short peptide signals. However, only few of them are functionally characterized and due to the small gene size and high sequence variability, the comprehensive identification of such peptide-encoded genes is challenging. The CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION-RELATED (CLE) gene family encodes for short peptides that have a role in plant meristem maintenance,...

Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

Aura Maria Moreno-Echeverri, Eva Susnik, Dimitri Vanhecke, Patricia Taladriz-Blanco, Sandor Balog, Alke Petri-Fink & Barbara Rothen-Rutishauser
Abstract Background In the field of nanoscience there is an increasing interest to follow dynamics of nanoparticles (NP) in cells with an emphasis on endo-lysosomal pathways and long-term NP fate. During our research on this topic, we encountered several pitfalls, which can bias the experimental outcome. We address some of these pitfalls and suggest possible solutions. The accuracy of fluorescence microscopy methods has an important role in obtaining insights into NP interactions with lysosomes at...

Registration Year

  • 2022
    4

Resource Types

  • Collection
    4

Affiliations

  • University of Fribourg
    4
  • Swiss Institute of Bioinformatics
    2
  • Adolphe Merkle Institute
    2
  • International Iberian Nanotechnology Laboratory
    2