2,342 Works

Traditional Chinese medicine Lingguizhugan decoction treating non-alcoholic fatty liver disease with spleen-yang deficiency pattern: Study protocol for a multicenter randomized controlled trial

Jingjuan Xu, Ruirui Wang, Shengfu You, Lei Zhang, Peiyong Zheng, Guang Ji & Baocheng Liu
Abstract Background Non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease characterized by excessive fat accumulation in the liver. One of the underlying pathophysiological mechanisms is insulin resistance (IR). Traditional Chinese medicine (TCM) has showed potential benefits in the management of NAFLD. Lingguizhugan decoction (LGZG) is a representative Chinese herbal formula; however, there is still no rigorous clinical trial supporting its application. Methods/design This study will be a three-arm, dose-optimization, randomized, double-blinded, placebo-controlled...

Low intratumor heterogeneity correlates with increased response to PD-1 blockade in renal cell carcinoma

Xia Ran, Jinyuan Xiao, Yi Zhang, Huajing Teng, Fang Cheng, Huiqian Chen, Kaifan Zhang & Zhongsheng Sun
Background:Intratumor heterogeneity (ITH) has been shown to be inversely associated with immune infiltration in several cancers including clear cell renal cell carcinoma (ccRCC), but it remains unclear whether ITH is associated with response to immunotherapy (e.g. PD-1 blockade) in ccRCC.Methods:We quantified ITH using mutant-allele tumor heterogeneity, investigated the association of ITH with immune parameters in patients with ccRCC (n = 336) as well as those with papillary RCC (pRCC, n = 280) from The Cancer...

Transcriptional dynamics of transposable elements when converting fibroblast cells of Macaca mulatta to neuroepithelial stem cells

Dahai Liu, Li Liu, Kui Duan, Junqiang Guo, Shipeng Li, Zhigang Zhao, Xiaotuo Zhang, Nan Zhou & Yun Zheng
Abstract Background Transposable elements (TE) account for more than 50% of human genome. It has been reported that some types of TEs are dynamically regulated in the reprogramming of human cell lines. However, it is largely unknown whether some TEs in Macaca mulatta are also regulated during the reprogramming of cell lines of monkey. Results Here, we systematically examined the transcriptional activities of TEs during the conversion of Macaca mulatta fibroblast cells to neuroepithelial stem...

Effect of preoperative CT angiography examination on the clinical outcome of patients with BMI ≥ 25.0 kg/m2 undergoing laparoscopic gastrectomy: study protocol for a multicentre randomized controlled trial

Cheng Meng, Shougen Cao, Xiaodong Liu, Leping Li, Qingsi He, Lijian Xia, Lixin Jiang, Xianqun Chu, Xinjian Wang, Hao Wang, Xizeng Hui, Zuocheng Sun, Shusheng Huang, Quanhong Duan, Daogui Yang, Huanhu Zhang, Yulong Tian, Zequn Li & Yanbing Zhou
Abstract Background Gastric cancer, which is the fifth most common malignancy and the third most common cause of cancer-related death, is particularly predominant in East Asian countries, such as China, Japan and Korea. It is a serious global health issue that causes a heavy financial burden for the government and family. To our knowledge, there are few reports of multicentre randomized controlled trials on the utilization of CT angiography (CTA) for patients who are histologically...

Effect of preoperative CT angiography examination on the clinical outcome of patients with BMI ≥ 25.0 kg/m2 undergoing laparoscopic gastrectomy: study protocol for a multicentre randomized controlled trial

Cheng Meng, Shougen Cao, Xiaodong Liu, Leping Li, Qingsi He, Lijian Xia, Lixin Jiang, Xianqun Chu, Xinjian Wang, Hao Wang, Xizeng Hui, Zuocheng Sun, Shusheng Huang, Quanhong Duan, Daogui Yang, Huanhu Zhang, Yulong Tian, Zequn Li & Yanbing Zhou
Abstract Background Gastric cancer, which is the fifth most common malignancy and the third most common cause of cancer-related death, is particularly predominant in East Asian countries, such as China, Japan and Korea. It is a serious global health issue that causes a heavy financial burden for the government and family. To our knowledge, there are few reports of multicentre randomized controlled trials on the utilization of CT angiography (CTA) for patients who are histologically...

Linc00152 promotes malignant progression of glioma stem cells by regulating miR-103a-3p/FEZF1/CDC25A pathway

Mingjun Yu, Yixue Xue, Jian Zheng, Xiaobai Liu, Hai Yu, Libo Liu, Zhen Li & Yunhui Liu
Abstract Background Glioma is one of the most frequent intracranial malignant tumors. LncRNAs have been identified as new modulators in the origination and progression of glioma. Methods Quantitative real-time PCR were conducted to evaluate the expression of linc00152 and miRNA-103a-3p in glioma tissues and cells. Western blot were used to determine the expression of FEZF1 and CDC25A in glioma tissues and cells. Stable knockdown of linc00152 or over-expression of miR-103a-3p in glioma stem cells (GSCs)...

A successful hybrid deep learning model aiming at promoter identification

Ying Wang, Qinke Peng, Xu Mou, Xinyuan Wang, Haozhou Li, Tian Han, Zhao Sun & Xiao Wang
Abstract Background The zone adjacent to a transcription start site (TSS), namely, the promoter, is primarily involved in the process of DNA transcription initiation and regulation. As a result, proper promoter identification is critical for further understanding the mechanism of the networks controlling genomic regulation. A number of methodologies for the identification of promoters have been proposed. Nonetheless, due to the great heterogeneity existing in promoters, the results of these procedures are still unsatisfactory. In...

Ultraviolet-to-Infrared Broadband Photodetector and Imaging Application based on Perovskite Single Crystal

Meng Xu, Xinyue Wang, Jiexin Weng, Jingling Shen, Yanbing Hou & Bo Zhang
The organic-inorganic hybrid perovskite CH3NH3PbBr3(MAPbBr3) has been well developed in the X-ray to visible light band due to its superior optoelectronic properties, but this material is rarely studied in the infrared band. In this paper, a UV-NIR broadband optical detector based on MAPbBr3 single crystal is studied, and the response range can reach the near-infrared region. In the visible light band, the optical response of the device is mainly caused by the photoelectric effect; in...

Ultraviolet-to-Infrared Broadband Photodetector and Imaging Application based on Perovskite Single Crystal

Meng Xu, Xinyue Wang, Jiexin Weng, Jingling Shen, Yanbing Hou & Bo Zhang
The organic-inorganic hybrid perovskite CH3NH3PbBr3(MAPbBr3) has been well developed in the X-ray to visible light band due to its superior optoelectronic properties, but this material is rarely studied in the infrared band. In this paper, a UV-NIR broadband optical detector based on MAPbBr3 single crystal is studied, and the response range can reach the near-infrared region. In the visible light band, the optical response of the device is mainly caused by the photoelectric effect; in...

Study on the prognosis, immune and drug resistance of m6A-related genes in lung cancer

Yang Yang, Zhouyao Qian, Mingyang Feng, Weiting Liao, Qiuji Wu, Feng Wen & Qiu Li
Abstract Background Few studies have demonstrated that the relationship between m6A-related genes and the prognosis, tumor microenvironment and drug resistance of LC. Methods The main results were analyzed with bioinformatics methods. Results Hence, we found 10 m6A-related genes expressed less in tumor samples in comparison with normal ones. Using consensus clustering, all LC patients were grouped into 2 subgroups according to the overall expression of 10 differential expressed m6A-related genes. In two clusters, the OS...

Genome-wide association study uncovers major genetic loci associated with flowering time in response to active accumulated temperature in wild soybean population

Guang Yang, Wei Li, Chao Fan, Miao Liu, Jianxin Liu, Wenwei Liang, Ling Wang, Shufeng Di, Chao Fang, Haiyang Li, Guohua Ding, Yingdong Bi & Yongcai Lai
Abstract Flowering time and active accumulated temperature (AAT) are two key factors that limit the expanded production especially for soybean across different regions. Wild soybean provides an important germplasm for functional genomics study in cultivar soybean. However, the studies on genetic basis underlying flowering time in response to AAT especially in wild soybean were rarely reported. In this study, we used 294 wild soybean accessions derived from major soybean production region characterized by different AAT...

Quantitative assessment of the effects of massive nucleic acid testing in controlling a COVID-19 outbreak

Wenlong Zhu, Yue Zhu, Zexuan Wen, Bo Zheng, Ao Xu, Ye Yao & Weibing Wang
Abstract Background From 20 July to 26 August 2021, local outbreaks of COVID-19 occurred in Nanjing City and Yangzhou City (Jiangsu Province, China). We analyzed the characteristics of these outbreaks in an effort to develop specific and effective intervention strategies. Methods Publicly available data on the characteristics of the COVID-19 outbreaks in Jiangsu Province were collected. Logistic regression was used to assess the association of age and sex with clinical severity. Analysis of onset dates,...

Transcriptomic and metabolomic analyses for providing insights into the influence of polylysine synthetase on the metabolism of Streptomyces albulus

Congcong Lian, Min Zhang, Jiaqi Mao, Yuanyu Liu, Xiuwen Wang, Linghui Kong, Qingshou Yao & Jiayang Qin
Abstract ε-poly-l-lysine (ε-PL) is the main secondary metabolite of Streptomyces albulus, and it is widely used in the food industry. Polylysine synthetase (Pls) is the last enzyme in the ε-PL biosynthetic pathway. Our previous study revealed that Pls overexpressed in S. albulus CICC11022 result in the efficient production of ε-PL. In this study, a Pls gene knockout strain was initially constructed. Then, genomic, transcriptomic and metabolomic approaches were integrated to study the effects of the...

Diagnostic models for fever of unknown origin based on 18F-FDG PET/CT: a prospective study in China

Jia Chen, MingYou Xing, Dong Xu, NaNa Xie, WenCong Zhang, QiuRong Ruan & JianXin Song
Abstract Background This study aims to analyze the 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) characteristics of different causes of fever of unknown origin (FUO) and identify independent predictors to develop a suitable diagnostic model for distinguishing between these causes. A total of 524 patients with classical FUO who underwent standard diagnostic procedures and PET/CT were prospectively studied. The diagnostic performance of PET/CT imaging was analyzed, and relevant clinical parameters that could improve diagnostic efficacy...

Analysis of different adipose depot gene expression in cachectic patients with gastric cancer

Jun Han, Zuoyou Ding, Qiulin Zhuang, Lei Shen, Fan Yang, Szechun Sah & Guohao Wu
Abstract Purpose This study aimed to identify the differentially expressed genes (DEGs) that contributed to the different amount of fat loss between subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) among cachectic patients. Methods RNA sequencing was performed and bioinformatic tools were utilized to analyze the biological functions and construct regulation networks of DEGs. We presumed that iroquois homeobox 1 (IRX1) to be a hub gene and analyzed its clinical significance. Mouse model of...

Analysis of different adipose depot gene expression in cachectic patients with gastric cancer

Jun Han, Zuoyou Ding, Qiulin Zhuang, Lei Shen, Fan Yang, Szechun Sah & Guohao Wu
Abstract Purpose This study aimed to identify the differentially expressed genes (DEGs) that contributed to the different amount of fat loss between subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) among cachectic patients. Methods RNA sequencing was performed and bioinformatic tools were utilized to analyze the biological functions and construct regulation networks of DEGs. We presumed that iroquois homeobox 1 (IRX1) to be a hub gene and analyzed its clinical significance. Mouse model of...

Fecal microbiota dynamics and its relationship to diarrhea and health in dairy calves

Hongwei Chen, Yalu Liu, Kailang Huang, Bin Yang, Yuanyuan Zhang, Zhongtang Yu & Jiakun Wang
Abstract Background Diarrhea is a major cause of morbidity and mortality in young calves, resulting in considerable economic loss for dairy farms. To determine if some gut microbes might have resistance to dysbiotic process with calf diarrhea by dictating the microbial co-occurrence patterns from birth to post-weaning, we examined the dynamic development of the gut microbiota and diarrhea status using two animal trials, with the first trial having 14 Holstein dairy calves whose fecal samples...

Transcriptomic and metabolomic analyses for providing insights into the influence of polylysine synthetase on the metabolism of Streptomyces albulus

Congcong Lian, Min Zhang, Jiaqi Mao, Yuanyu Liu, Xiuwen Wang, Linghui Kong, Qingshou Yao & Jiayang Qin
Abstract ε-poly-l-lysine (ε-PL) is the main secondary metabolite of Streptomyces albulus, and it is widely used in the food industry. Polylysine synthetase (Pls) is the last enzyme in the ε-PL biosynthetic pathway. Our previous study revealed that Pls overexpressed in S. albulus CICC11022 result in the efficient production of ε-PL. In this study, a Pls gene knockout strain was initially constructed. Then, genomic, transcriptomic and metabolomic approaches were integrated to study the effects of the...

Association of physical activity and air pollution exposure with the risk of type 2 diabetes: a large population-based prospective cohort study

Zhi-Hao Li, Wen-Fang Zhong, Xi-Ru Zhang, Vincent CH Chung, Wei-Qi Song, Qing Chen, Xiao-Meng Wang, Qing-Mei Huang, Dong Shen, Pei-Dong Zhang, Dan Liu, Yu-Jie Zhang, Pei-Liang Chen, Xin Cheng, Hai-Lian Yang, Miao-Chun Cai, Xiang Gao, Virginia Byers Kraus & Chen Mao
Abstract Background The interplay between physical activity (PA) and air pollution in relation to type 2 diabetes (T2D) remains largely unknown. Based on a large population-based cohort study, this study aimed to examine whether the benefits of PA with respect to the risk of T2D are moderated by exposure to air pollution. Methods UK Biobank participants (n = 359,153) without diabetes at baseline were included. Information on PA was obtained using the International Physical Activity...

Low barrier medication for opioid use disorder at a federally qualified health center: a retrospective cohort study

Jamie Carter, Zhen Li, Hillary Chen, Melissa Greiner, Christopher Bush, Debanjan Bhattacharya, Stephanie Poley, Nidhi Sachdeva, Jane Carolyn Crowder & Jacob Feigal
Abstract Background Medication for opioid use disorder (MOUD) reduces mortality, but few patients access MOUD. At a Federally Qualified Health Center (FQHC), we implemented a low barrier model of MOUD, including same-day MOUD initiation and a harm reduction philosophy. Objective To investigate whether low barrier MOUD improved retention in care compared to traditional treatment. Design and participants Retrospective cohort study of patients with at least one visit seeking MOUD at the FQHC during a historical...

SIRPα antibody combined with oncolytic virus OH2 protects against tumours by activating innate immunity and reprogramming the tumour immune microenvironment

Defeng Kong, Zhenrong Yang, Guoliang Li, Quanyou Wu, Zhaoru Gu, Duo Wan, Qi Zhang, Xiaoli Zhang, Shujun Cheng, Binlei Liu, Kaitai Zhang & Wen Zhang
Abstract Background The combination of oncolytic viruses (OVs) with immune checkpoint blockades is a research hotspot and has shown good efficacy. Here, we present the first attempt to combine oncolytic herpes simplex virus 2 (OH2) with an anti-SIRPα antibody as an antitumour treatment. Our results provide unique insight into the combination of innate immunity with OV. Methods We verified the polarization and activation of OH2 in RAW264.7 cells in vitro. Subsequently, we evaluated the antitumour...

Myofascial release for the treatment of pain and dysfunction in patients with chronic mechanical neck pain: Systematic review and meta-analysis of randomised controlled trials

Yaorui Guo, Xiuying Lv, Yu Zhou, Zhijun Li, Huiping She, Li Bai & Jing Bao
ObjectiveTo explore the effects of myofascial release (MFR) on pain and dysfunction in individuals with chronic mechanical neck pain (MNP).Data sourcesPubMed, Embase, Medline, Wiley Online Library, Web of Science, CNKI, VIP, WanFang Data, and the Cochrane Library were searched until 12 September 2022.Review methodsThis study was registered in PROSPERO (CRD42022302485). Methodological quality was assessed using Cochrane risk of bias assessment, and the quality of the evidence followed the GRADE recommendation. The outcomes pain, cervical mobility...

Application of exome sequencing for prenatal diagnosis of fetal structural anomalies: clinical experience and lessons learned from a cohort of 1618 fetuses

Fang Fu, Ru Li, Qiuxia Yu, Dan Wang, Qiong Deng, Lushan Li, Tingying Lei, Guilan Chen, Zhiqiang Nie, Xin Yang, Jin Han, Min Pan, Li Zhen, Yongling Zhang, Xiangyi Jing, Fucheng Li, Fatao Li, Lina Zhang, Cuixing Yi, Yingsi Li, Yan Lu, Hang Zhou, Ken Cheng, Jian Li, Lina Xiang … & Can Liao
Abstract Background Exome sequencing (ES) is becoming more widely available in prenatal diagnosis. However, data on its clinical utility and integration into clinical management remain limited in practice. Herein, we report our experience implementing prenatal ES (pES) in a large cohort of fetuses with anomalies detected by ultrasonography using a hospital-based in-house multidisciplinary team (MDT) facilitated by a three-step genotype-driven followed by phenotype-driven analysis framework. Methods We performed pES in 1618 fetal cases with positive...

Genome-wide identification of key enzyme-encoding genes and the catalytic roles of two 2-oxoglutarate-dependent dioxygenase involved in flavonoid biosynthesis in Cannabis sativa L.

Xuewen Zhu, Yaolei Mi, Xiangxiao Meng, Yiming Zhang, Weiqiang Chen, Xue Cao, Huihua Wan, Wei Yang, Jun Li, Sifan Wang, Zhichao Xu, Atia Tul Wahab, Shilin Chen & Wei Sun
Abstract Background Flavonoids are necessary for plant growth and resistance to adversity and stress. They are also an essential nutrient for human diet and health. Among the metabolites produced in Cannabis sativa (C. sativa), phytocannabinoids have undergone extensive research on their structures, biosynthesis, and biological activities. Besides the phytocannabinoids, C. sativa is also rich in terpenes, alkaloids, and flavonoids, although little research has been conducted in this area. Results In this study, we identified 11...

Establishment and evaluation of a CT-based radiomic model for AIDS-associated pulmonary cryptococcosis

Zi-xin Zhang, Xing-yu Mu, Jing Yu, Chun-shuang Guan, Bu-dong Chen & Ru-Ming Xie
Abstract Background Establish a CT-based diagnostic radiomic model for AIDS complicated with pulmonary cryptococcosis and evaluate the diagnostic efficacy of this model. Methods This retrospective study enrolled 98 AIDS patients with pulmonary cryptococcosis and 103 AIDS patients with other infections or neoplastic lesions, comprising a total of 699 lesions. Patients were randomly divided into a training group and test group at a ratio of 2.75:1. Features from all lesions, cavity lesions and solid nodule lesions...

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